Understanding the response of preferential flow paths to water movement is an important topic in soil hydrology. However, quantification of the complicated distribution patterns of preferential flow paths remains poorly understood. Therefore, dye experiments were conducted to investigate preferential flow characteristics under three different precipitation amounts (20, 40 and 60 mm, numbered as the G20, G40 and G60, respectively) in Simian Mountain grassland, Chongqing province, China. O-ring statistics were used to analyze the spatial distribution characteristics and the spatial correlation of preferential flow paths. Results revealed that precipitation could promote dye tracer infiltration into deeper soils, reaching the maximum depth of 55 cm in G60. The number of preferential flow paths in G60 plots was 3.0 and 7.4 times greater than those of G40 and G20, respectively. Structural distribution of the preferential flow paths showed a gradually clumped pattern with the increase of precipitation, which was conducive to enhancing the correlation between preferential flow paths in each pore size range. These results could expand our understanding of the effects of precipitation on the characteristic of preferential flow paths in grassland, which is helpful to evaluate the water movement in the study area.