Complex Lifestyle Research Articles

Role of the type 6 secretion system on apoptosis and macrophage polarization during Burkholderia pseudomallei infection.

Burkholderia pseudomallei (Bpm) is the causative agent of the disease melioidosis. As a facultative intracellular pathogen, Bpm has a complex lifestyle that culminates in cell-to-cell fusion and multinucleated giant cells (MNGCs) formation. The virulence factor responsible for MNGC formation is the type 6 secretion system (T6SS), a contractile nanomachine. MNGC formation is a cell-to-cell spread strategy that allows the bacteria to avoid the extracellular immune system and our previous data highlighted cell death, apoptosis, and inflammation as pathways significantly impacted by T6SS activity. Thusly, we investigated how the T6SS influences these phenotypes within the macrophage and pulmonary models of infection. Here we report that the T6SS is responsible for exacerbating apoptotic cell death during infection in both macrophages and the lungs of infected mice. We also demonstrate that although the T6SS does not influence differential macrophage polarization, the M2 polarization observed is potentially beneficial for Bpm pathogenesis and replication. Finally, we show that the T6SS contributes to the severity of inflammatory nodule formation in the lungs, which might be potentially connected to the amount of apoptosis that is triggered by the bacteria.

Nutrition and fasting

Patients with rheumatic diseases often enquire about the options for nutritional therapy. Nutritional factors have been empirically described that are associated with the occurrence of inflammatory rheumatic diseases or flare-ups or improved disease states. Agrowing number of epidemiological and clinical studies deal with the evaluation of nutrition and dietary restriction in rheumatology. Narrative presentation of the evidence of nutritional interventions and fasting and its clinical implications. Only limited data from smaller clinical studies are available for evidence assessment. Abenefit in terms of symptoms and quality of life in rheumatoid arthritis was shown for the Mediterranean and plant-based diet as well as the anti-inflammatory diet. The effect sizes are small to moderate and the effectiveness in the context of complex lifestyle programs is probably sustainable. The evidence for elimination diets is weak. Initial clinical studies indicate amoderate benefit of plant-based nutrition for osteoarthritis in the context of the metabolic syndrome. There is moderate evidence for the benefit of dietary weight normalization in psoriasis. There is clear experimental evidence of asignificant anti-inflammatory effect of prolonged fasting. Several clinical studies demonstrated asymptomatic benefit of prolonged modified fasting (therapeutic fasting) in rheumatoid arthritis (RA). If fasting is followed by avegan and vegetarian diet, lasting effects of up to 1year have been documented. Cardiometabolic but not antirheumatic effects have been proven for intermittent fasting. Nutrition and fasting can be classified as apossible useful addition to conventional treatment but are currently only rarely taken into account in practice.

Uncovering the hidden complexity of multicellular magnetotactic bacteria.

Multicellular magnetotactic bacteria (MMB) have a surprisingly complex multicellular lifestyle. A new study in PLOS Biology combines genomics, microscopy, and isotopic labeling to show that MMB form obligately multicellular consortia of genetically diverse cells with rudimentary division of labor.

Mid- and late-life lifestyle activities as main drivers of general and domain-specific cognitive reserve in individuals with Parkinson’s disease: cross-sectional and longitudinal evidence from the LANDSCAPE study

BackgroundCognitive reserve (CR) is considered a protective factor for cognitive function and may explain interindividual differences of cognitive performance given similar levels of neurodegeneration, e.g., in Alzheimer´s disease. Recent evidence suggests that CR is also relevant in Parkinson’s disease (PD).ObjectiveWe aimed to explore the role of life-stage specific CR for overall cognition and specific cognitive domains cross-sectionally and longitudinally in PD.MethodsThe cross-sectional analysis with data from the DEMPARK/LANDSCAPE study included 81 individuals without cognitive impairment (PD-N) and 87 individuals with mild cognitive impairment (PD-MCI). Longitudinal data covered 4 years with over 500 observations. CR was operationalized with the Lifetime of Experiences Questionnaire (LEQ), capturing the complexity of lifestyle activities across distinct life-stages. Cognition was assessed using a comprehensive neuropsychological test battery.ResultsHigher LEQ scores, particularly from mid- and late-life, were observed in PD-N compared to PD-MCI [F(1,153) = 4.609, p = .033, ηp2 = 0.029]. They were significantly associated with better cognitive performance (0.200 ≤ β ≤ 0.292). Longitudinally, linear mixed effect models (0.236 ≤ marginal R2 ≤ 0.441) revealed that LEQ scores were positively related to cognitive performance independent of time. However, the decline in overall cognition and memory over time was slightly more pronounced with higher LEQ scores.ConclusionsThis study emphasizes the association between complex lifestyle activities and cognition in PD. Data indicate that while CR might be related to a delay of cognitive decline, individuals with high CR may experience a more pronounced drop in overall cognition and memory. Future studies will have to replicate these findings, particularly regarding domain-specific effects and considering reverse causal mechanisms.

Toxoplasma and Plasmodium associate with host Arfs during infection.

The parasites Toxoplasma gondii and Plasmodium live complex intracellular lifestyles where they must acquire essential host nutrients while avoiding recognition. Although previous work has sought to identify the specific nutrients scavenged by apicomplexans, the mechanisms by which host materials are transported to and across the parasite vacuole membrane are largely unknown. Here, we examined members of the host vesicular trafficking network to identify specific pathways subverted by T. gondii and Plasmodium berghei. Our results indicate that T. gondii selectively internalizes host Arfs, a class of proteins involved in intracellular trafficking. For P. berghei, host Arfs were restricted by the parasite's vacuole membrane, but proteins involved in vesicular trafficking were identified as essential for liver stage development. A greater exploration into how and why apicomplexans subvert host vesicular trafficking could help identify targets for host-directed therapeutics.

Factors Explaining the Willingness of Small-Scale Private Forest Owners to Engage in Forestry—A German Case Study

In Germany, 24% of the forest area is owned by small-scale private forest owners, whose property is affected by a societal demand for forest-related ecosystem services. In the inhomogeneous group of small-scale private forest owners, different living conditions and lifestyles exist, which are affecting the management of the forest ownership. To support the management activities of small-scale private forest owners, institutional instruments exist, but these are only used by a small proportion of forest owners. In order to gain insights into the accessibility and activation of small-scale private forest owners for management, and to draw conclusions for appropriate support instruments, a large number of studies have analyzed the factors influencing the management activities of forest owners. Some studies identify socio-demographic and socio-economic variables as important influencing factors, but do not take into account their complex interdependencies, which have developed as a result of the pluralization of lifestyles and individualization of forest owners. For this reason, in addition to the influencing factors identified in the literature as relevant for forest management activities of small-scale private forest owners, the lifestyle segmentation approach of Sinus milieus established in German market research was applied in this study. This approach allocates forest owners based on their complex lifestyles into ten different milieu groups. A logistic regression analysis was used to determine the parameters influencing the willingness to engage in eight different forest management activities. The results show that the willingness to engage in forest management activities in the future is higher compared to actions in the past. In addition to timber harvesting-related activities, the small-scale private forest owners show a high action potential for non-commodity-related activities. Non-commodity-related activities can primarily be explained by the Sinus milieu affiliation of the small-scale private forest owners. This result underlines the usefulness of a lifestyle segmentation approach such as the Sinus milieus to address small-scale private forest owners in a target group specific way in line with their willingness to engage in specific forest management activities. Institutional instruments such as the membership in a forest owners association or subsidies do not play a significant role for non-commodity-related activities and seem to be inappropriate for targeting small-scale private forest owners for these activities. Timber harvesting-related activities are primarily influenced by the presence of professional foresters, socio-demographic factors or institutional instruments. Based on these empirical findings, implications can be drawn for the target group-specific addressing of forest owners based on different management activities. In this way, forest owners can be motivated to engage in forest management and the provision of forest-related ecosystem services.

A latent trajectory analysis of young sexual and gender minorities’ adherence to three rectal microbicide placebo formulations (MTN-035; a randomized crossover trial)

BackgroundRectal microbicides (RM) are biomedical HIV prevention products that aim to prevent or reduce the transmission of HIV and other sexually transmitted infections (STIs). RM modalities may be beneficial for populations who have complex lifestyles, difficulties adhering to pre-exposure prophylaxis (PrEP) regimens, and/or have limited access to care. MTN-035 (DESIRE; Developing and Evaluating Short-Acting Innovations for Rectal Use), a randomized crossover trial, aimed to evaluate the safety and acceptability of, and adherence to, three placebo RM modalities (douche, insert, and suppository) prior to receptive anal intercourse.MethodsWe conducted latent trajectory analysis to identify clusters of individuals who shared similar trajectories in acceptability and adherence for each product (douche, insert, and suppository) over time. We analyzed weekly short messaging service (SMS) use reports for each modality as reported by enrolled sexual and gender minority (SGM) participants.ResultsTwo trajectories for each product were identified: a “protocol compliant” trajectory (i.e., at least one product use occasion per week) and “high use” trajectory (i.e., more than three product use occasions per week). Participants with high use were more likely to lack access to PrEP and have higher intentions to utilize RM modalities compared to those who were protocol compliant.ConclusionsThis study highlighted high adherence to RM modalities among SGM. As research into viable HIV prevention modalities continues to evolve, tailored intervention strategies are needed to support the uptake of and adherence to alternative prevention modalities that are behaviorally congruent with targeted users.Trial registrationNCT03671239 (14/09/2018).

UnDerstandIng the cauSes of mediCation errOrs and adVerse drug evEnts for patients with mental illness in community caRe (DISCOVER): a qualitative study.

It is estimated that 237 million medication errors occur in England each year with a significant number occurring in the community. Our understanding of the causes of preventable medication errors and adverse drug events (ADE) affecting patients with mental illness is limited in this setting. Better understanding of the factors that contribute to errors can support the development of theory-driven improvement interventions. Remote qualitative semi-structured interviews with 26 community-based healthcare professionals in England and Wales were undertaken between June-November 2022. Recruitment was undertaken using purposive sampling via professional networks. Interviews were guided by the critical incident technique and analysed using the framework method. Any data that involved speculation was not included in the analysis. Independent analysis was carried out by the research team to extract themes guided by the London Protocol. A total of 43 medication errors and 12 preventable ADEs were discussed, with two ADEs having an unknown error origin. Prescribing errors were discussed most commonly (n = 24), followed by monitoring errors (n = 8). Six contributory factor themes were identified: the individual (staff); the work environment; the teams/interfaces; the organisation and management; the patient; and the task and technology. The individual (staff) factors were involved in just over 80% of all errors discussed. Participants reported a lack of knowledge regarding psychotropic medication and mental illnesses which accompanied diffusion of responsibility. There were difficulties with team communication, particularly across care interfaces, such as ambiguity/brevity of information being communicated and uncertainty concerning roles which created confusion amongst staff. Unique patient social/behavioural contributory factors were identified such as presenting with challenging behaviour and complex lifestyles, which caused difficulties attending appointments as well as affecting overall clinical management. These findings highlight that the causes of errors are multifactorial with some unique to this patient group. Key areas to target for improvement include the education/training of healthcare professionals regarding neuropharmacology/mental illnesses and enhancing communication across care interfaces. Future research should explore patient perspectives regarding this topic to help develop a holistic picture. These findings can be used to guide future intervention research to ameliorate medication safety challenges for this patient group.

Biological control of anthracnose fruit rot disease (Colletotrichum spp.) and fruit yield improvement of jujube (Zizyphus jujuba Miller var. inermis Rehder) using Bacillus velezensis CE 100

Colletotrichum spp. are notorious phytopathogens that cause anthracnose rot disease in jujube fruits, have a complex lifestyle (with necrotrophic, hemibiotrophic, quiescent, and endophytic phases), they spread rapidly mainly through the production of spores and are increasingly becoming resistant to various fungicides which makes them difficult to control. This study investigated the inhibition of spore germination and mycelial growth of Colletotrichum spp. to suppress anthracnose rot disease in jujube fruits through the activity of cell wall degrading enzymes, and improvement of jujube fruit characteristics and yield via the production of indole-3-acetic acid (IAA) and gibberellin acid (GA3) by Bacillus velezensis CE 100. The bacterial culture broth exhibited a strong chitinase, β-1,3-glucanase and protease and the crude fraction suppressed the germination of spores by more than 95.0 % at a concentration of 100 µL/mL, and substantially reduced mycelial growth of Colletotrichum spp. The microscopic analysis revealed that the treatments degraded the cell walls and caused severe structural distortion of the spores and hyphae of Colletotrichum spp. Field application of B. velezensis CE 100 on the jujube tree crown and in the rhizosphere lowered the rate of anthracnose disease incidence by 2.8-fold and disease severity index (DSI) by 4.4-fold, compared to the control. In addition, B. velezensis CE 100 produced IAA (up to 15.6 mg/mL) and GA3 (up to 1.3 mg/mL), and the field application of the bacterial culture on the jujube tree crown and in the rhizosphere consequently improved the average fruit characteristics (fruit length, fruit diameter and fresh weight by 1.2-, 1.4- and 1.5-fold), chlorophyll index (photosynthetic rate by 1.1-fold), and fruit yield (by 2.1-fold) of jujube, compared to the control group. Thus, the inoculation of B. velezensis CE 100 has the potential effectiveness as a bio-fungicide against anthracnose rot disease in jujube fruits, and as a bio-fertilizer to improve fruit characteristics and yield.

The heparin-binding hemagglutinin protein of Mycobacterium tuberculosis is a nucleoid-associated protein

Nucleoid-associated proteins (NAPs) regulate multiple cellular processes such as gene expression, virulence, and dormancy throughout bacterial species. NAPs help in the survival and adaptation of Mycobacterium tuberculosis (Mtb) within the host. Fourteen NAPs have been identified in Escherichia coli; however, only seven NAPs are documented in Mtb. Given its complex lifestyle, it is reasonable to assume that Mtb would encode for more NAPs. Using bioinformatics tools and biochemical experiments, we have identified the heparin-binding hemagglutinin (HbhA) protein of Mtb as a novel sequence-independent DNA-binding protein which has previously been characterized as an adhesion molecule required for extrapulmonary dissemination. Deleting the carboxy-terminal domain of HbhA resulted in a complete loss of its DNA-binding activity. Atomic force microscopy showed HbhA-mediated architectural modulations in the DNA, which may play a regulatory role in transcription and genome organization. Our results showed that HbhA colocalizes with the nucleoid region of Mtb. Transcriptomics analyses of a hbhA KO strain revealed that it regulates the expression of ∼36% of total and ∼29% of essential genes. Deletion of hbhA resulted in the upregulation of ∼73% of all differentially expressed genes, belonging to multiple pathways suggesting it to be a global repressor. The results show that HbhA is a nonessential NAP regulating gene expression globally and acting as a plausible transcriptional repressor.

Detection of bacterial and protozoan pathogens in individual bats and their ectoparasites using high-throughput microfluidic real-time PCR.

Among the most studied mammals in terms of their role in the spread of various pathogens with possible zoonotic effects are bats. These are animals with a very complex lifestyle, diet, and behavior. They are able to fly long distances, thus maintaining and spreading the pathogens they may be carrying. These pathogens also include vector-borne parasites and bacteria that can be spread by ectoparasites such as ticks and bat flies. In the present study, high-throughput screening was performed and we detected three bacterial pathogens: Bartonella spp., Neoehrlichia mikurensis and Mycoplasma spp., and a protozoan parasite: Theileria spp. in paired samples from bats (blood and ectoparasites). In the samples from the bat-arthropod pairs, we were able to detect Bartonella spp. and Mycoplasma spp. which also showed a high phylogenetic diversity, demonstrating the importance of these mammals and the arthropods associated with them in maintaining the spread of pathogens. Previous studies have also reported the presence of these pathogens, with one exception, Neoehrlichia mikurensis, for which phylogenetic analysis revealed less genetic divergence. High-throughput screening can detect more bacteria and parasites at once, reduce screening costs, and improve knowledge of bats as reservoirs of vector-borne pathogens. IMPORTANCE The increasing number of zoonotic pathogens is evident through extensive studies and expanded animal research. Bats, known for their role as reservoirs for various viruses, continue to be significant. However, new findings highlight the emergence of Bartonella spp., such as the human-infecting B. mayotimonensis from bats. Other pathogens like N. mikurensis, Mycoplasma spp., and Theileria spp. found in bat blood and ectoparasites raise concerns, as their impact remains uncertain. These discoveries underscore the urgency for heightened vigilance and proactive measures to understand and monitor zoonotic pathogens. By deepening our knowledge and collaboration, we can mitigate these risks, safeguarding human and animal well-being.

Being alone, together: How does design shape social interactions and relationships of young adults in Singapore

Studying the social interactions and relationships of young adults in Singapore, this paper seeks to understand the issue of social disconnection and how design shapes them. Young adults face the challenge of navigating the complex lifestyle of a smart city while balancing their ever expanding and fluctuating social network. As such, it causes them to feel disconnected in their social interactions and relationships. By considering these questions: What does it mean to be connected? What is the role of product design in people’s relationships? Why do young adults feel disconnected in a technologically connected society? How can design establish a truly connected society? it will evaluate how products could be designed and created for genuine interactions and fulfilling relationships. From the surveys, interviews, observations, case studies and literature readings in this study, it reveals the social disconnection young adults face in a connected city, how they navigate in a technological environment, and how experiences are designed for connection. It also shows that design plays a vital role in tackling the social disconnection young adults face. Designing with a human-centric approach not only creates an opportunity for people to create meaningful relationships using technology, but also maintains the spontaneous and fluid elements of social connections through prompting interactions. Additionally, it has been found that design is only a platform where they can initiate these interactions and relationships, the decision to disconnect remain with the human user as much as devices have been designed to lead to certain decisions being made.

Homelessness: measuring need to design more inclusive services

PurposeThe purpose of this paper – the second of three – is to report the findings from a service user needs assessment in those who have contact with a local homelessness service in the North-West of England.Design/methodology/approachData were collected using a semi-structured questionnaire that included a section exploring Adverse Childhood Experiences. Aggregated data from a total of 100 completed questionnaires were analysed to understand the nature and scope of those accessing the Homelessness and Vulnerable Adults Service (HVAS).FindingsHomeless people accessing HVAS face a number of challenges, which reflect their upbringing and chaotic and complex lifestyles. Reports of multiple disadvantage, social isolation, physical and mental health problems were common among the cohort.Research limitations/implicationsThis was a small cohort study, and the authors accept that this may potentially limit the scope of the findings. Themes identified are, however, reflected in wider research and official data collection sources. Future research may seek to widen the data collection methods to offer a more representative cohort.Practical implicationsThe provision of co-ordinated multi-agency support is essential to tackle health inequalities experienced by those who are homeless.Social implicationsThe complex issues often experienced by those who are homeless can further compound the impact of social exclusion on health and well-being. The reduction of statutory support and increased emphasis on self-reliance can further impact those people on the margins of society.Originality/valueThis study identifies how multiple deprivations and social isolation impacts upon health and well-being, further compounding a person’s ability and willingness to engage with services. It raises the question of the systems failure to respond effectively.

Comparative genomic and phenotypic analyses of pathogenic fungi Neoscytalidium dimidiatum and Bipolaris papendorfii isolated from human skin scraping.

Neoscytalidium dimidiatum and Bipolaris species are fungal plant pathogens that have been reported to cause human diseases. Recently, we have isolated numerous N. dimidiatum and Bipolaris species from the skin scrapings and nails of different patients. In this work, we have sequenced the genome of one strain of N. dimidiatum. The sequenced genome was compared to that of a previously reported Bipolaris papendorfii genome for a better understanding of their complex lifestyle and broad host-range pathogenicity. Both N. dimidiatum UM 880 (~ 43Mb) and B. papendorfii UM 226 (~ 33Mb) genomes include 11,015-12,320 putative coding DNA sequences, of which 0.51-2.49% are predicted transposable elements. Analysis of secondary metabolism gene clusters revealed several genes involved in melanin biosynthesis and iron uptake. The arsenal of CAZymes related to plants pathogenicity is comparable between the species, including genes involved in hemicellulose and pectin decomposition. Several important gene encoding keratinolytic peptidases were identified in N. dimidiatum and B. papendorfii, reflecting their potential pathogenic role in causing skin and nail infections. In this study, additional information on the metabolic features of these two species, such as nutritional profiling, pH tolerance, and osmotolerant, are revealed. The genomic characterization of N. dimidiatum and B. papendorfii provides the basis for the future functional studies to gain further insights as to what makes these fungi persist in plants and why they are pathogenic to humans.

Comparative Genomic Analysis of Cold-Water Coral-Derived Sulfitobacter faviae: Insights into Their Habitat Adaptation and Metabolism

Sulfitobacter is one of the major sulfite-oxidizing alphaproteobacterial groups and is often associated with marine algae and corals. Their association with the eukaryotic host cell may have important ecological contexts due to their complex lifestyle and metabolism. However, the role of Sulfitobacter in cold-water corals remains largely unexplored. In this study, we explored the metabolism and mobile genetic elements (MGEs) in two closely related Sulfitobacter faviae strains isolated from cold-water black corals at a depth of ~1000 m by comparative genomic analysis. The two strains shared high sequence similarity in chromosomes, including two megaplasmids and two prophages, while both contained several distinct MGEs, including prophages and megaplasmids. Additionally, several toxin-antitoxin systems and other types of antiphage elements were also identified in both strains, potentially helping Sulfitobacter faviae overcome the threat of diverse lytic phages. Furthermore, the two strains shared similar secondary metabolite biosynthetic gene clusters and genes involved in dimethylsulfoniopropionate (DMSP) degradation pathways. Our results provide insight into the adaptive strategy of Sulfitobacter strains to thrive in ecological niches such as cold-water corals at the genomic level.

Identifying latent activity behaviors and lifestyles using mobility data to describe urban dynamics

Urbanization and its problems require an in-depth and comprehensive understanding of urban dynamics, especially the complex and diversified lifestyles in modern cities. Digitally acquired data can accurately capture complex human activity, but it lacks the interpretability of demographic data. In this paper, we study a privacy-enhanced dataset of the mobility visitation patterns of 1.2 million people to 1.1 million places in 11 metro areas in the U.S. to detect the latent mobility behaviors and lifestyles in the largest American cities. Despite the considerable complexity of mobility visitations, we found that lifestyles can be automatically decomposed into only 12 latent interpretable activity behaviors on how people combine shopping, eating, working, or using their free time. Rather than describing individuals with a single lifestyle, we find that city dwellers’ behavior is a mixture of those behaviors. Those detected latent activity behaviors are equally present across cities and cannot be fully explained by main demographic features. Finally, we find those latent behaviors are associated with dynamics like experienced income segregation, transportation, or healthy behaviors in cities, even after controlling for demographic features. Our results signal the importance of complementing traditional census data with activity behaviors to understand urban dynamics.

Comparative genomics and transcriptomics insight into myxobacterial metabolism potentials and multiple predatory strategies.

Myxobacteria are part of the phylum Myxococcota, encompassing four orders. Most of them display complex lifestyles and broad predation profiles. However, metabolic potential and predation mechanisms of different myxobacteria remains poorly understood. Herein, we used comparative genomics and transcriptomics to analyze metabolic potentials and differentially expressed gene (DEG) profiles of Myxococcus xanthus monoculture (Mx) compared to coculture with Escherichia coli (MxE) and Micrococcus luteus (MxM) prey. The results showed that myxobacteria had conspicuous metabolic deficiencies, various protein secretion systems (PSSs) and the common type II secretion system (T2SS). RNA-seq data demonstrated that M. xanthus overexpressed the potential predation DEGs, particularly those encoding T2SS, the tight adherence (Tad) pilus, different secondary metabolites (myxochelin A/B, myxoprincomide, myxovirescin A1, geosmin and myxalamide), glycosyl transferases and peptidase during predation. Furthermore, the myxalamide biosynthesis gene clusters, two hypothetical gene clusters and one arginine biosynthesis clusters were highly differential expressed in MxE versus MxM. Additionally, homologue proteins of the Tad (kil) system and five secondary metabolites were in different obligate or facultative predators. Finally, we provided a working model for exhibiting multiple predatory strategies when M. xanthus prey on M. luteus and E. coli. These results might spur application-oriented research on the development of novel antibacterial strategies.

Rapid metabolic reprogramming mediated by the AMP-activated protein kinase during the lytic cycle of Toxoplasma gondii

The ubiquitous pathogen Toxoplasma gondii has a complex lifestyle with different metabolic activities at different stages that are intimately linked to the parasitic environments. Here we identified the eukaryotic regulator of cellular homeostasis AMP-activated protein kinase (AMPK) in Toxoplasma and discovered its role in metabolic programming during parasite’s lytic cycle. The catalytic subunit AMPKα is quickly phosphorylated after the release of intracellular parasites to extracellular environments, driving energy-producing catabolism to power parasite motility and invasion into host cells. Once inside host cells, AMPKα phosphorylation is reduced to basal level to promote a balance between energy production and biomass synthesis, allowing robust parasite replication. AMPKγ depletion abolishes AMPKα phosphorylation and suppresses parasite growth, which can be partially rescued by overexpressing wildtype AMPKα but not the phosphorylation mutants. Thus, through the cyclic reprogramming by AMPK, the parasites’ metabolic needs at each stage are satisfied and the lytic cycle progresses robustly.

Role of loneliness in destination emotional values and travel intentions: analysis of solo women travellers in India

While millennial women are living the fastest and most complex lifestyles, loneliness has emerged as a distinct problem among them. However, travel promotes identity, education and aids in giving meaning and purpose to people's lives. Against this background, the present study aims towards establishing a link between loneliness and travel intentions among millennial women in India. The overall relationship was tested and it was found that the emotional values related to the destination play an important role. The relationship was elicited from the responses of Tier I cities - Delhi, Mumbai, Kolkata and Chennai. In addition, the feeling of loneliness has been found positively influencing the emotional values that tourist seek which further significantly influences their intentions to travel towards a destination.

Stress Responses in Pathogenic Vibrios and Their Role in Host and Environmental Survival.

Vibrio is a genus of bacteria commonly found in estuarine, marine, and freshwater environments. Vibrio species have evolved to occupy diverse niches in the aquatic ecosystem, with some having complex lifestyles. About a dozen of the described Vibrio species have been reported to cause human disease, while many other species cause disease in other organisms. Vibrio cholerae causes epidemic cholera, a severe dehydrating diarrheal disease associated with the consumption of contaminated food or water. The human pathogenic non-cholera Vibrio species, Vibrio parahaemolyticus and Vibrio vulnificus, cause gastroenteritis, septicemia, and other extra-intestinal infections. Infections caused by V. parahaemolyticus and V. vulnificus are normally acquired through exposure to sea water or through consumption of raw or undercooked contaminated seafood. The human pathogenic Vibrios are exposed to numerous different stress-inducing agents and conditions in the aquatic environment and when colonizing a human host. Therefore, they have evolved a variety of mechanisms to survive in the presence of these stressors. Here we discuss what is known about important stress responses in pathogenic Vibrio species and their role in bacterial survival.