Complete Replacement Of Soybean Meal Research Articles

Evaluation of four novel non-grain protein sources completely replacing soybean meal on growth performance, serum biochemistry, amino acid transport and intestinal health of grass carp (Ctenopharyngodon idella) at different water temperatures

To investigate the effects of non-grain protein source and water temperature on growth and feed utilization differences of grass carp, the effects of different protein sources on the growth performance, serum biochemistry, digestive enzymes, amino acid transport and intestinal health of grass carp were studied at 24 °C, 28 °C and 32 °C. In this study, a total of 1350 grass carp (Ctenopharyngodon idella) (initial weight 5.00 ± 0.02 g) were selected, and Clostridium autoethanogenum protein (CAP), Tenebrio molitor meal (TMM), cottonseed protein concentrate (CPC) and Chlorella powder (CHP) were used as a single protein source to completely replace soybean meal for 56 days. The results showed that the final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR) and protein efficiency ratio (PER) of grass carp increased significantly with the increasing temperature (P < 0.001). The CHP and SBM groups showed no significant differences in FBW, WGR, SGR and PER (P > 0.05), which were higher than the CAP, TMM and CPC groups (P < 0.001). The alanine transaminase (ALT), aspartate aminotransferase (AST), total protein (TP) and triglyceride (TG) concentrations of grass carp at 32 °C were significantly lower than those at 24 °C and 28 °C (P < 0.001). The acid phosphatase (ACP) activity decreased significantly with the increase of temperature (P = 0.001). The amylase (AMS) activity of the TMM, CPC and CHP groups was significantly lower than that of the SBM and CAP groups (P < 0.001), and the ACP and lipase (LPS) activities in the TMM group were significantly lower than those in the SBM group (P < 0.001). In addition, the interaction between temperatures and protein sources significantly affected the gene expression levels of amino acid transport including solute carrier family 1 member 3 (SLC1A3), solute carrier family 7 member 1 (SLC7A1), solute carrier family 7 member 5 (SLC7A5), solute carrier family 15 member 1b (SLC15A1b), solute carrier family 7 member 7 (SLC7A7), target of rapamycin (TOR), 4E binding protein 1 (4E-BP1) and ribosomal protein S6 kinase 1 (S6K1), intestinal inflammatory including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-8 (IL-8), interleukin-10 (IL-10) and tight junction proteins (occludin, claudin1, claudin3, claudin7 and claudin11) (P ≤ 0.001). Collectively, our results indicated that CHP could be a potential protein source in the case of complete replacement of soybean meal in grass carp.

Dietary vitamin E further enhances the effectiveness of Clostridium autoethanogenum protein as an alternative source of soybean meal in grass carp (Ctenopharygodon idellus) diets based on growth performance, flesh quality and antioxidant status

The effects of replacing soybean meal with Clostridium autoethanogenum protein and supplementing it with different gradients of vitamin E in grass carp diets on growth performance, flesh quality and antioxidant capacity were evaluated in a 69-day feeding trial. Five isonitrogen and isolipid diets were configured with the soybean meal-based diet (SM) served as the basal control diet, and the Clostridium autoethanogenum protein-based diet (CAP) as the treatment control diet. The CAP diet was supplemented with 80 mg/kg, 160 mg/kg and 240 mg/kg vitamin E (namely, 80VE, 160VE and 240VE), respectively. Final body weight (FBW) and specific growth rate (SGR) were significantly better in the CAP group than in the SM group (P < 0.05), and the 160 VE group was significantly better than the CAP group (P < 0.05). In terms of muscle quality, muscle fat content was significantly higher in the CAP group than in the SM group (P < 0.05), and muscle vitamin E content was significantly higher in each vitamin E-supplemented group than in the CAP group (P > 0.05). The total odor activity value in muscle in the CAP group was not significantly different from the SM group (P < 0.05), but the vitamin E-supplemented groups were significantly higher than the CAP group (P < 0.05). Muscle hardness and gumminess were significantly higher in the CAP group than the SM group and muscle shear force and gumminess were significantly higher in the 80VE group than the CAP group (P < 0.05). Muscle development-related genes in the CAP group were not significantly different from those in the SM group (P > 0.05), and Muscle Myf5 mRNA levels in the 160VE and 240VE groups were significantly higher than those in the CAP group, while the expression of MSTN genes was suppressed (P < 0.05). In terms of antioxidant properties, muscle MDA content was significantly higher in the CAP group than in the SM group (P < 0.05), and muscle MDA content was significantly lower in the 160VE and 240VE groups than in the CAP group (P < 0.05), at the same time, CAT and T-SOD activities were significantly higher (P < 0.05). In conclusion, complete replacement of soybean meal by the Clostridium autoethanogenum protein in grass carp diets promoted growth performance and muscle quality, but negatively affected antioxidant capacity, and supplementation of 160 mg/kg vitamin E in the CAP diet could further enhance growth performance and improve antioxidant capacity.

Complete replacement of soybean meal with black soldier fly larvae meal in feeding program for broiler chickens from placement through to 49 days of age: impact on gastrointestinal, breast, skeletal, plasma, and litter attributes

We previously reported that high levels (≥50%) of black soldier fly larvae meal (BSFLM) reduced growth and altered organs morphology in broiler chickens. We further examined gastrointestinal, breast, bone, plasma, and litter attributes in broiler chickens fed partial to complete replacement of soybean meal (SBM) with BSFLM. A total of 1,152 d-old Ross × Ross 708 male chicks (n=8) were allocated to 48 pens and assigned one of six diets: a basal corn-SBM diet (0BSFLM), four diets in which SBM in 0BSFLM was replaced with BSFLM at 12.5, 25, 50, and 100% (12.5BSFLM, 25BSFLM, 50BSFLM, 100BSFLM) and a final diet (0+AGP) in which 0BSFLM was fed with coccidiostat and antibiotic. Birds were bled for plasma and necropsied for samples. Litter samples were collected on d 45 to 47. Breast weight, woody breast, and hardness scores and tibia morphometry reduced linearly in response to BSFLM inclusion (P&lt;0.001). BSFLM linearly increased plasma Lys, Met, Thr, uric acid, creatine kinase and decreased Arg and potassium (P&lt;0.05). Litter ammonium nitrogen and potassium decreased linearly in response to BSFLM (P&lt;0.001). In conclusion, the data suggested physiological and metabolic inefficiencies in broiler chickens fed isocaloric and isonitrogenous diets with ≥50% BSFLM replacement of SBM.

246 Evaluation of the Complete Replacement of Soybean Meal by Rapeseed Meal on Performance and Carcass Composition of Grow-Finishing Pigs

Abstract The aim of this experiment was to evaluate the effects of replacing soybean meal (SBM) by rapeseed meal (RSM) as main protein source in diets for grow-finishing pigs. The trial included a total of 1,248 crossbred boars and gilts [Pietrain x (Landrace x Large White), with an initial body weight (BW) of 38.3 ± 5.29 kg (Mean ± SD)], distributed in 96 pens of 13 pigs. Pens were randomly distributed in 2 dietary treatments, balanced by BW, block and sex within each of the 8 slurry pits. The inclusion of SBM and RSM in the growing diets was 13.8% and 20.0%, whereas in the finishing diets (days 52-91) was 10.5% and 14.4%, respectively. Diets were formulated to have the same net energy (NE) value and crude protein differences were below 0.5%. To achieve isoenergetic diets, the inclusion of animal fat was increased by 2.4% and 1.7% in RSM diets compared with SBM for the growing and finishing phases, respectively. Standardized ileal digestible (SID) Lys was kept at 0.95% and 0.86% in the growing and finishing phases, respectively. Performance variables were analyzed by using a linear mixed model with treatment and sex as fixed effects and initial BW as covariate, while pit was included as a random effect. Individual carcass data were analyzed in a linear mixed model with treatment, sex and marketing group as fixed effects, and hot carcass weight as a covariate, and pen within a pit as random effect. During the growing phase, pigs showed lower ADFI when fed the RSM diet (1.68 vs. 1.62 kg; P = 0.002) that resulted in decreased ADG (0.842 vs. 0.803 kg; P = 0.015), but FCR was not affected (2.00 vs. 2.02; P = 0.459). Contrarily, in the finishing phase, ADFI was not significantly reduced (1.98 vs. 1.95 kg; P = 0.586) but less FCR was observed when feeding RSM (2.59 vs. 2.49; P = 0.007). Final BW was not significantly affected (112.0 vs. 110.7 kg; P = 0.404), but hot carcass weight tended to be less in RSM fed pigs (92.2 vs. 91.3 kg; P = 0.078). No significant differences were reported on carcass yield (P = 0.815), carcass leanness (P = 0.850) or backfat thickness (P = 0.850). In conclusion, feeding growing pigs diets without SBM might negatively affect their performance during the growing phase, but those effects can be partly counteracted in the finishing phase without modifying carcass composition.

Effects of partial or complete replacement of soybean meal with commercial black soldier fly larvae (Hermetia illucens) meal on growth performance, cecal short chain fatty acids, and excreta metabolome of broiler chickens

Black soldier fly larvae meal (BSFLM) is receiving great attention as a rich source of protein and antimicrobials for poultry. Therefore, we evaluated the effects of partially or completely replacing soybean meal (SBM) with commercial BSFLM on growth performance, tibia traits, cecal short chain fatty acid (SCFA) concentrations, and excreta metabolomes in broiler chickens (Gallus gallus domesticus). A total of 480 day-old male Ross×Ross 708 chicks were assigned to 6 diets (8 replicates/diet): a basal corn-SBM diet with in-feed bacitracin methylene disalicylate (BMD), a corn-SBM diet without BMD (0% BSFLM), and four diets in which the SBM was substituted with 12.5, 25, 50, and 100% BSFLM. Body weight (BW), feed intake (FI) and cumulative feed conversion ratio (cFCR) were monitored on days 14, 28, and 35. Cecal SCFA levels were determined on days 14, 28, and 35. Tibia traits and excreta metabolomes were determined on day (d) 35. On d14, birds fed 12.5 and 25% BSFLM had a similar BW, FI, and cFCR as birds fed BMD (P > 0.05). On d 35, birds fed 12.5% BSFLM had a similar BW, FI and cFCR as birds fed BMD or 0% BSFLM (P > 0.05). For each phase, birds fed 100% BSFLM had a lower BW, FI and higher cFCR than birds fed BMD or 0% BSFLM (P < 0.05). On d 35, BW decreased linearly, quadratically, and cubically with increasing levels of BSFLM (P < 0.01). Overall (d 0-35), BSFLM linearly, quadratically, and cubically decreased FI and quadratically and cubically increased cFCR (P < 0.01). Quadratic responses were observed for tibia fresh weight (P=0.049) and ash content (P=0.022). BSFLM did not impact cecal SCFAs levels. The excreta metabolome of birds fed 100% BSFLM clustered independently from all other groups and exhibited greater levels of putatively identified methionine, lysine, valine, glutamine, histidine and lower levels of arginine as compared to all diets. Taken together, substitution of SBM with ≤25% of BSFLM in the starter phase may be used as an alternative to BMD.

Effectiveness of using high-protein sunflower concentrate in pig feeding

The article studied the dependence of productive qualities and feed cost on the growth of pigs during rearing and fattening with partial (50 %) and complete (100 %) replacement of soybean meal with high-protein protein concentrate “Proglot” in the diet. It was established that the use of partial and complete replacement of soybean meal with high-protein sunflower concentrate in the rearing of piglets led to a decrease in the growing intensity of piglets by 0.87 % with partial and 4.95 % with total replacement. And as a result of a decrease in live weight by 0.3 % at the end of the growing period with a partial and by 1.2 % with a complete replacement of soybean products with sunflower in the starter compound feed. At the same time, feed conversion turned out to be the best in the group of animals that partially replaced soybean meal with high-protein sunflower concentrate – 2.30 kg, which is 1.41 % better than in animals that consumed soybean meal in the starter feed and by 5.37 % compared to animals in which this meal was completely replaced by high-protein sunflower concentrate. Replacing soybean meal with high-protein sunflower concentrate made it possible to reduce the cost of 1 kg of feed by 2.24 % when it was partially replaced and by 4.57 % when it was replaced entirely. The feed cost of 1 kg of growth in piglets that consumed feed with a partial replacement of soybean meal with high-protein sunflower concentrate decreased by 3.64 % and by 3.47 % in animals that completely replaced soybean meal with this product. Animals whose diet was replaced entirely with soybean meal by high-protein sunflower concentrate had the lowest feed cost per head. According to this indicator, they outnumbered peers with partial replacement by 3.83 % and 4.33 %, where such replacement was not done. Animals whose diet was replaced entirely with soybean meal by high-protein sunflower concentrate had the lowest feed cost per head for the entire experiment period. According to this indicator, they were 3.14 % better than their peers, partially replaced by 5.25 %. It was not carried out. The index of fattening qualities of pigs was the highest in pigs with partial replacement of soybean meal with high-protein sunflower concentrate, 31.2 points. In contrast, in animals with the complete replacement, it was 9.75 % lower, and by 2.74 %, and in analogs with such replacement was not conducted. A clear tendency to increase the intensity of growth of piglets was established with the combined use (50/50 %) of soybean meal and high-protein sunflower concentrate. However, the use of only high-protein sunflower concentrate probably reduces this indicator. It was determined that replacing soybean meal with high-protein protein concentrate “Proglot” reduces the cost of feed and, as a result, the cost of feed per unit of growth and per head of pigs after fattening.

Complete replacement of soybean meal with black soldier fly larvae meal in feeding program for broiler chickens from placement through to 49 days of age reduced growth performance and altered organs morphology

Black soldier fly larvae meal (BSFLM) is characterized with good nutritional and functional attributes. However, there is limited data on inclusion of BSFLM in broiler chicken rations from placement through to market weight. Therefore, we examined growth and organ responses of partial to complete replacement of soybean meal (SBM) with BSFLM in a practical feeding program. A total of 1,152 d-old male Ross × Ross 708 chicks were allocated to 48 pens and assigned one of six diets (n = 8). The diets were: a basal corn-SBM diet (0%BSFLM), 4 diets in which SBM in 0%BSFLM was replaced with BSFLM at 12.5, 25, 50, and 100% and a final diet (0 + AGP) in which 0%BSFLM was treated with coccidiostat (70 mg Narasin/kg) and antibiotic (55 mg Bacitracin Methylene Disalicylate/kg). For energy fortification, soy oil was used for 0%BSFLM diets and black soldier fly oil in the other diets. Body weight, feed intake (FI), BW gain (BWG), and mortality-corrected feed conversion ratio (FCR) were reported. Organ weights were recorded on d 24 and 49. On d 10, birds fed diets 12.5, 25, and 0 + AGP had higher BWG than birds fed diets 0, 50, and 100 (P < 0.01), and birds fed diet 100 had lower BWG than birds fed diets 0 or 50 (P < 0.01). Birds fed diets 50 and 100 had lower BWG than birds fed all other diets on d 24 and 49 (P < 0.05). Overall (d 0–49), BSFLM linearly (P < 0.01) decreased BW, BWG, and FI and increased FCR and mortality. The overall BWG of 50 and 100% BSFLM birds was 92 and 81% of birds fed 0%BSFLM, repectively and coresponding overall FI was 96 and 90%. An increase in gizzard, small intestine, pancreas, and liver relative weights were observed with increasing BSFLM inclusion (P < 0.01). The data indicated that lower levels of BSFLM could provide some growth-promoting effects commensurate to antibiotics in the starter phase. However, replacing SBM with greater amounts (≥50) of BSFLM reduced growth and increased organ size.

Partial Replacement of Soybean Meal with Canola Meal or Corn DDGS in Low-Protein Diets Supplemented with Crystalline Amino Acids-Effect on Growth Performance, Whole-Body Composition, and Litter Characteristics.

Simple SummaryDietary protein reduction with amino acid supplementation is a nutritional approach to reducing environmental pollution. Soybean meal is nutritionally superior to alternative protein feedstuffs, such as corn distillers dried grain with solubles (cDDGS) and canola meal (CM), because of its relatively good balance of amino acids. This study investigated the usage of CM or cDDGS compared to SBM in low-protein broiler diets and their effects on growth performance, carcass yield, whole-body composition, and litter characteristics. The results demonstrated that replacing soybean meal with cDDGS or CM negatively affected the performance of the birds, whereas reducing dietary protein reduced the litter surface ammonia, irrespective of protein feedstuff. Therefore, complete replacement of SBM with cDDGS or CM in low-protein diets is not feasible for optimum performance in broiler chickens.A 42-day study was conducted to explore the application of supplemental amino acids (AA) in low-protein diets with soybean meal (SBM), canola meal (CM) or corn distillers dried grain with solubles (cDDGS) as the main protein feedstuffs. The responses of interest were growth performance, carcass yield, whole-body composition, litter ammonia and litter N. On d 0, a total of 540 Cobb 500 (off-sex) male broilers were allocated to 36 floor pens. All the birds received one starter diet that met nutrient requirements during the first 10d. Thereafter, six experimental diets were provided in grower and finisher phases. The diets included a positive control (PC): a corn–SBM diet with adequate protein. The protein level of the negative control (NC) was decreased by 45 g/kg relative to the PC. The next two diets had the same protein levels as the NC but with cDDGS added at 50 or 125 g/kg. The last two diets had the same CP as the NC but with CM added at 50 or 100 g/kg. All the low-protein diets had the same level of standardized ileal digestible indispensable AA according to Cobb 500 recommended level. Gly and Ser were added as sources of non-specific N. The dietary protein reduction in corn–SBM diets at both phases decreased (p < 0.05) weight gain and increased (p < 0.05) feed conversion ratio (FCR). Increasing levels of cDDGS or CM, at a constant CP level, linearly decreased (p < 0.05) the weight gain and feed intake, whereas increasing CM level linearly increased (p < 0.05) FCR in the grower and finisher phases. The eviscerated and carcass yields decreased, whereas the fat yield increased (p < 0.05) with reduced protein in corn–SBM diet. Increasing levels of cDDGS and CM at a constant CP level quadratically decreased (p < 0.05) the eviscerated weight, whereas the fat weight linearly decreased (p < 0.05) with increasing levels of cDDGS and CM. The birds receiving the PC diet had a lower (p < 0.05) lean muscle (%) and a higher fat (%) compared to birds receiving the NC diet at d 21. However, on d42, birds receiving the PC diet had decreased (p < 0.05) bone mineral density, bone mineral content and lean weight compared to those receiving the NC diet. The litter ammonia increased (p < 0.05) with the increasing levels of protein in the SBM diets. In conclusion, 50 g/kg inclusion levels of CM and cDDGS at the same low-protein levels as SBM produced a similar growth response to the NC, whereas higher levels were detrimental. Hence under the conditions of the current experiment, complete replacement of SBM with DDGS or CM in low-protein diets was not feasible.

Complete replacement of soybean meal with defatted black soldier fly larvae meal in Shaver White hens feeding program (28–43 wks of age): impact on egg production, egg quality, organ weight, and apparent retention of components

Effects of total replacement of soybean meal (SBM) with defatted black soldier fly larvae meal (BSFLM) on egg production and quality, organ weight, and apparent retention (AR) of components were investigated in Shaver White hens from 28 to 43 wk of age. A total of 108 birds, (6 birds/cage) were assigned to three diets (6 replicates/diet). Diets were control corn–SBM diet and two additional diets made with the addition of either 10 or 15% BSFLM. Diets met or exceeded breeder specifications, contained TiO2 as an indigestible marker, and were prepared in pellet form. Birds had free access to feed and water throughout the experiment. Hen-day egg production (HDEP) was monitored daily. Feed intake (FI) and body weight (BW) were monitored in 4-wk intervals. All eggs laid on the sixth day of wks 31, 35, 39, and 43 were used for egg weight (EW), Haugh units (HU), yolk color (YC), shell breaking strength (SBS), and shell thickness (ST). Excreta samples were collected for 3 consecutive days on wk 33 for AR and two birds/cage were necropsied at the end. There were no (P > 0.05) diet effects on HDEP, FI, and HU. Inclusion of BSFLM linearly decreased (P < 0.05) egg mass and feed conversion ratio (FCR) and quadratically increased (P < 0.05) BW. There was no (P > 0.05) interaction between diet and sampling time point on egg quality parameters. Inclusion of BSFLM increased SBF and YC linearly (P < 0.05) and ST quadratically (P = 0.028). Inclusion of BSFLM quadratically (P ≤ 0.01) reduced empty ceca weight and increased liver weight and had no effect (P > 0.05) on gizzard, small intestine, and pancreas weights. Feeding BSFLM linearly (P = 0.001) and quadratically (P = 0.007) increased apparent metabolizable energy (AME). Data showed that defatted BSFLM resulted in deeper orange yolks and improved eggshell quality; however, unfavorable FCR linked to lighter eggs as well as heavier birds and liver warrants further investigations.

Growth performance and meat composition of rabbits fed diets supplemented with silkworm pupae meal

Aim of study: To determine the effect of different dietary levels of silkworm pupae meal (SWPM) on the growth performance of broiler rabbits and the chemical composition of their meat.Area of study: Southern Poland, Europe.Material and methods: Ninety Termond White rabbits were divided into three feeding groups. Control group rabbits were fed a diet containing 10% soybean meal (SBM). In the first experimental group, rabbits received a diet containing 5% SBM and 5% SWPM. The diet administered to the second experimental group was supplemented with 10% SWPM.Main results: Both partial and complete replacement of SBM with SWPM in diets contributed to a decrease in the final body weights of rabbits (2416.50 and 2390.78 vs. 2616.78 g), average daily gains (30.23 and 30.52 vs. 33.12 g) and feed intake (5.61 and 5.15 vs. 6.51 kg), but it improved the feed conversion ratio (3.28 and 3.05 vs. 3.41 kg/kg). Rabbits fed diets supplemented with SWPM were characterized by lower values of selected carcass parameters such as carcass weight and dressing percentage. Experimental diets had no significant effect on the proximate chemical composition of meat, but they increased the levels of fatty acids C18:3, C22:5 and C22:6.Research highlights: The results of this study can be put into practice to replace conventional protein and fat sources in rabbit diets with insect meals (SWPM) in the production of rabbit meat for human consumption. Our findings can also contribute to the recycling of sericultural waste.

Fermented soybean meal ameliorates Salmonella Typhimurium infection in young broiler chickens

The present study was designed to investigate the effectiveness of dietary fermented soybean meal (FSBM) in comparison with prebiotic (Xylooligosaccharide; XOS) and probiotic (Lactic acid bacteria-based probiotic; LAC) for prevention of Salmonella Typhimurium (ST) infection in young broiler chickens from 1 to 24 d. The in vitro study revealed that soybean meal (SBM) fermentation increased the number of lactic acid bacteria (LAB) and lactic acid content and inhibited the growth of enterobacteria such as coliforms in SBM. A total of 450 day-old Ross 308 broiler chicks were placed in 30 pens (15 birds/pen) and allocated to 5 experimental treatments that consisted an un-supplemented basal diet and not infected (NC) or infected with ST (IC); IC plus 2g XOS/kg; IC plus 0.2g LAC/kg; and IC containing a complete replacement of SBM with FSBM. All birds (except NC) were orally administered with 0.5mL of the ST solution (1 × 106 CFU/mL) at d 3 post-hatch. The ST challenge decreased body weight gain and feed intake (P < 0.05). The impairment of feed conversion ratio was alleviated by the addition of XOS, LAC, and FSBM in broiler diets compared with IC birds (P < 0.05). The ST infection reduced duodenum and jejunum villus height and increased Salmonella colonization throughout the gut as well as internal organ invasion compared with NC birds (P < 0.05). However, ST-infected broilers fed the XOS, LAC, and FSBM-containing diets showed a significant decrease in gut Salmonella colonization, and internal organ invasion, an increase in LAB counts, and improvement in intestinal mucosa morphology (P < 0.05). The tested feed additives or FSBM reduced heterophil to lymphocyte ratio compared with the IC group (P < 0.05). The results suggest that XOS, LAC, and FSBM improve growth performance, lower Salmonella colonization, and improve intestinal characteristics and immune response in ST-challenged broiler chicks. Therefore, fermented feeds due to having functional ingredients can be considered as an effective strategy to lessen the colonization of gut pathogens in broiler chickens.

Nutritional impact of partial or complete replacement of soybean meal by sesame (Sesamum indicum) meal in lambs rations

BackgroundOne of the important by-products is sesame seed meal; it is a by-product of sesame seed pressing. Sesame oil cake or meal is a relatively good source of crude protein which can replace part of basic ingredients in diets such as soybean.MethodFifteen growing male Barki lambs aged 5–6 months (18.50 ± 0.98 kg) were used to investigate the influence of replacing soybean meal (SBM) that incorporated (16% of control ration) sesame meal (SM) at 50% or 100% on feed and water intakes, nutrient digestibility, growth performance, rumen parameters, and economic evaluation. Lambs received one of the three tested complete feed mixtures that contained 16% SBM (R1), replaced 50% of SBM with SM (R2), contained (8% SBM + 8% SM) or completely replaced 100% of SBM with SM (R3) and contained 16% SM.ResultsDietary treatments had no significant effect on all nutrient digestibility and total digestible nutrients value, meanwhile it decreased (P < 0.05) their contents of digestable crud protine when SBM was completely replaced by SM. Average daily gain (ADG) increased (P < 0.05) while increasing the level of replacement SBM by SM. Feed conversion expressed as g. gain improved (P < 0.05) with the increasing level of inclusion SM in the rations. Ruminal pH values increased (P < 0.05), meanwhile, values of NH3-N concentration insignificantly decreased; however, values of total volatile fatty acid concentration insignificantly increased when SBM was replaced at half or completely by SM. Economical efficiency improved by 147.9% and 163.5% for R2 and R3 compared to control (R1).ConclusionIt can be mentioned that SM is a good source of protein and can be successfully used as an unconventional source in growing lamb rations without causing any deleterious effect on their performance, digestibility, and ruminal fermentation while realizing a decrease in feed cost with improving economic efficiency, so it can incorporate SM in sheep rations to improve profitability or net revenue and decrease feed cost/kg gain.

Effect of supplementing canola quality rapeseed mustard cake on the metabolic profile and quality of milk in crossbred cows

This study examined the comparative effect of incorporation of improved canola quality and high glucosinolates rapeseed mustard oil cake (RMC) in the supplement of crossbred cows on metabolic profile, thyroid hormones, milk yield and quality of milk. Lactating crossbred cows (18) were randomly divided into three groups of six each in a completely randomized design and allocated in three dietary supplements, viz. SBM, RMC and CM containing soybean meal (SBM), rapeseed mustard cake (RMC) and canola meal (CM), respectively as a major protein source for a period of 90 days. Daily intake of total dry matter and green fodder was comparable irrespective of dietary treatments. The supplement intake (kg/d) was significantly lower in the cows that were given RMC supplement compared to those given either SBM or CM supplement. Incorporation of CM in the supplement significantly increased daily and total milk yield (kg) as compared to RMC group. The milk thiocyanate level was significantly higher in RMC followed by CM and SBM groups. Blood-biochemical profile except significantly higher lactate dehydrogenase in RMC group remained comparable at different periods. Serum thiocyanate was significantly higher in RMC followed by CM and SBM. Serum T3 and T4 hormone levels were significantly lower in RMC fed group as compared to CM and SBM groups. It can be concluded that feeding of canola quality RMC (GSC5/6) may be used as complete replacement of soybean meal without any adverse effect on metabolic profile, thyroid hormones, milk quality and yield in crossbred cows.

Does the Optimal Dietary Methionine to Cysteine Ratio in Diets for Growing Chickens Respond to High Inclusion Rates of Insect Meal from Hermetia illucens?

Simple SummaryCurrently, several alternative protein sources are under investigation for replacing soybean meal in poultry diets. One alternative is larvae meal of the black soldier fly (Hermetia illucens) with a specific sulfur amino acid composition. The larvae meal is limiting in sulfur amino acids supply and provides a wide methionine:cysteine ratio of 61:39. Currently, it is not known whether the insect meal has an impact on the optimal ratio of methionine to cysteine in broiler chicken diets. The methionine:cysteine ratio significantly influences animal growth and welfare. Both methionine and cysteine excess and deficiency can lead to an impaired feed intake, growth, and feed efficiency. Therefore, the aim of this study was to investigate whether the optimal methionine:cysteine ratio is modulated when a high inclusion rate of partly defatted Hermetia illucens meal is applied. The results show that a methionine:cysteine ratio of 50:50 yields superior growth and dietary protein quality. It can be concluded that the insect meal under study is a promising alternative protein source without modulating the optimal methionine:cysteine ratio in broiler chicken diets.The dietary methionine:cysteine (Met:Cys) ratio (MCR) is an important factor influencing the optimal growth of chickens. Therefore, this study aimed to contribute to the assessment of the optimal dietary MCR in diets with the complete replacement of soybean meal (SBM) by a partly defatted larvae meal of Hermetia illucens (HM). A growth study with 240 male meat-type chickens (Ross 308) was conducted, also assessing the body nutrient deposition both at the end of the starter (day 21) and the grower (day 35) period. Birds were fed experimental diets based on wheat, maize, and insect meal (23%/21% HM in starter/grower diets). Sulfur amino acids were created as the limiting AA in diets with graded MCR (40:60; 45:55; 50:50; 55:45; 60:40). The control diet contained SBM instead of HM with a MCR of 50:50. The current results based on growth parameters, dietary protein quality, and Met efficiency data gave support to the previous assumption of an ideal MCR of 50:50, which was also valid in diets with a high proportion of insect meal. The lowest MCR of 40:60 led to significantly impaired feed intake and growth of the birds, while the response to the highest MCR (60:40) was moderate.

Improving the Dietary Protein Quality by Amino Acid Fortification with a High Inclusion Level of Micro Algae (&amp;lt;i&amp;gt;Spirulina platensis&amp;lt;/i&amp;gt;) or Insect Meal (&amp;lt;i&amp;gt;Hermetia illucens&amp;lt;/i&amp;gt;) in Meat Type Chicken Diets

The objective of this study was to measure protein quality parameters (PPV, NPU and NPUstd) of chicken diets with complete substitution of soybean meal (SBM) by Spirulina meal (SM) or partly defatted Hermetia meal (HM). N balance experiments were based on the quantitative excreta collection method, divided into starter period (10 - 20 d) and grower period (25 - 35 d). The study utilized 70 all male meat type chicken (Ross 308). Data assessment applied the exponential N utilization model of the “Goettingen approach”. The control diet was based on wheat, corn and SBM. In four experimental diets SBM was completely substituted by SM or HM, but fortified with feed amino acids (AA) both on a basic level of supplementation (Lys and Met added equal to the control diet) and on an extended level (Lys, Met, Thr, Arg, Val, Ile, His added). At a basic level of AA supplementation, complete replacement of SBM by SM or HM in chicken diets depressed dietary protein quality significantly (p 0.05). However, the extended level of AA supplementation improved protein quality parameters of the diets with both of the alternative proteins significantly (p

Effect of dietary lupin (Lupinus albus) on the gastrointestinal microbiota composition in broiler chickens and ducks

The purpose of the study was to evaluate the amount of raffinose-series oligosaccharides (RSO) in soybean meal (SBM), whole white lupin seed meal (WLM), sunflower meal (SFM), and rapeseed oil meal (ROM) and to determine whether partial or complete dietary WLM replacement affected the numbers of bacteria in selected groups in the microbiota of broiler chickens and ducks without inducing any weight loss. Total counts of anaerobes, lactobacilli, bifidobacteria, and Escherichia coli in caecal samples from both ducks and broiler chickens, as well as in a crop chyme, in broiler chickens, were determined. Live weights before slaughter were determined. Both broiler chickens and ducks were fed a control diet with SBM (L&lt;sub&gt;0&lt;/sub&gt;) or diet containing 50% or 100% WLM as a substitute for SBM (groups L&lt;sub&gt;50&lt;/sub&gt; and L&lt;sub&gt;100&lt;/sub&gt;, respectively). In comparison with SBM, WLM contained significantly higher amounts of RSO, and the amounts of oligosaccharides in SFM (1.73 ± 0.26 g/100 g) and ROM (1.79 ± 0.14 g/100 g) were negligible compared to those in WLM (8.26 ± 0.14 g/100 g) and SBM (6.96 ± 0.21 g/100 g). The inclusion of lupin in chicken diets did not significantly affect the monitored bacterial groups in crop chyme, but a complete replacement of SBM with WLM (L&lt;sub&gt;100 &lt;/sub&gt;group) in chicken diets significantly (P ≤ 0.05) increased the counts of lactobacilli in caecal samples. Partial (L&lt;sub&gt;50 &lt;/sub&gt;group) and complete (L&lt;sub&gt;100 &lt;/sub&gt;group) lupin supplementation in the duck diet significantly (P ≤ 0.05) increased counts of lactobacilli and bifidobacteria by at least one order of magnitude. E. coli counts in poultry were not affected by changes in diet. The results of our study indicate that partial dietary replacement of SBM with WLM did not significantly affect the live weight of broiler chickens and ducks, but that complete replacement of SBM with WLM may lead to weight loss.

Productive performance and blood profiles of laying hens fed Hermetia illucens larvae meal as total replacement of soybean meal from 24 to 45 weeks of age

The aim of the research was to study the effects of an insect meal from Hermetia illucens larvae (HILM) as complete replacement of soybean meal (SBM) on productive performance and blood profiles of laying hens, from 24 to 45 wk of age. A total of 108 24-week-old Lohmann Brown Classic laying hens was equally divided into 2 groups (54 hens/group, 9 replicates of 6 hens/group). From 24 to 45 wk of age, the groups were fed 2 different isoproteic and isoenergetic diets: the control group (SBM) was fed a corn-soybean meal based diet, while in the HILM group the soybean meal was completely replaced by Hermetia illucens larvae meal. Feed intake, number of eggs produced, and egg weight were recorded weekly along the trial. At 45 wk of age, blood samples were collected from 2 hens per replicate. The use of HIML led to a more favorable (P <0.01) feed conversion ratio in hens but lay percentage, feed intake, average egg weight, and egg mass were higher (P <0.01) in hens fed the SBM diet. Hens fed insect meal produced a higher percentage of eggs from small (S), medium (M), and extra-large (XL) classes (P <0.01) than SBM, while the SBM group had a higher percentage of eggs from the large (L) class (P <0.01). The levels of globulin and albumin to globulin ratio were, respectively, higher and lower (P <0.05) in HILM than the SBM group. Cholesterol and triglycerides were higher (P <0.05 and P <0.01, respectively) in hens from SBM than in the HILM group. Blood levels of Ca were higher (P <0.01) in hens fed insect meal, while creatinine was higher (P <0.01) in blood of hens fed SBM. Hermetia illucens larvae meal can be a suitable alternative protein source for laying hens even if the complete replacement of soybean meal needs further investigation to avoid the negative effects on feed intake.

Effective utilization of distiller's grain soluble-an agro-industrial waste in the feed of cage-reared minor carp Labeo bata in a tropical reservoir, India.

A 60-day feeding trial was conducted to evaluate the efficacy of distiller's grain soluble (brewery waste) as a prospective ingredient to substitute expensive and high demand feed component, soybean meal for farming Labeo bata in cages installed in tropical reservoir. Two isonitrogenous and isocaloric diets comprising brewery waste (49.2% CP) as test diet and soybean meal (44.4% CP) as reference diet were formulated and extruded to obtain 2-mm floating pellets. The efficacy of the diets was tested in terms of survival (%), live weight gain (%), SGR (%/day), FCR, PER and ANPU and recorded 65 ± 0.2, 96 ± 8.1, 1.9 ± 0.1, 2.5 ± 0.02, 1.4 ± 0.1, 20.3 ± 2.0 and 66 ± 0.6, 112 ± 9.8, 2.2 ± 0.1, 2.2 ± 0.2, 1.6 ± 0.1 and 20 ± 2.1, respectively, for soybean and brewer's waste-based formulated feed. The analyses of results revealed that survival, growth parameters and biochemical composition of whole body tissue did not differ significantly (p > 0.05) despite complete replacement of soybean meal by brewery waste. However, the cost estimate of diet revealed marked reduction of feed cost of Rs. 9.2/kg (33.8%) in the test diet as compared to the reference diet. The study suggests that brewery waste could effectively replace soybean meal without effecting survival and growth of the fish. The finding thus may pave a productive way for reducing environmental pressure of disposal of an agro-industrial waste.

Use of larvae meal as protein source in broiler diet: Effect on growth performance, nutrient digestibility, and carcass and meat traits.

The aim of this research was to study the effect of insect meal from larvae ( larvae meal [TML]) as complete replacement of soybean meal (SBM) on growth performance, nutrient digestibility, and carcass and meat traits of broilers. A total of eighty 30-d-old male Shaver brown broilers were homogenously divided into 2 groups (each consisting of 8 replicates of 5 birds). Up to 62 d of age, the groups were fed 2 isoproteic and isoenergetic diets differing for the ingredient used as the main protein source: the control group was fed a corn-SBM-based diet, whereas in the TML group, the SBM was completely replaced by TML. Broiler growth performance was measured during the trial. At 62 d of age, 2 broilers per replicate (16 per group) were slaughtered and apparent ileal digestibility coefficients and carcass and meat traits were determined. The use of TML as the main protein source in the broiler diet had no significant effect on most growth performance and carcass traits and chemical and physical properties of meat, the latter being important for marketing purposes. The feed conversion ratio in the entire experimental period (from 30 to 62 d) was improved in the TML group compared with the SBM group ( < 0.05). The apparent ileal digestibility coefficients of DM, OM, and CP in broilers fed the SBM diet were greater ( < 0.01) than the other group. The full digestive system in broilers fed SBM had a lower ( < 0.05) absolute and relative weight than that of broilers fed TML. Also, the weight and the percentage of the spleen in the SBM group were lower ( < 0.05) than those in the TML group. The length of the entire intestine in the group fed TML was greater ( < 0.05) than the other group and the same happened when intestinal length was expressed as percentage of broiler BW ( < 0.05). Among the different intestinal tracts, the ileum and ceca of broilers fed TML had a greater ( < 0.05) length than that of broilers fed SBM. Also, ceca weight (as an absolute value or percentage on live weight) in broilers fed TML was greater ( < 0.05) than that of broilers fed SBM. Breast pH and cooking losses in broilers fed TML were greater ( < 0.05) than those of broilers fed SBM. Chemical characteristic of meat obtained from right breast of slaughtered broilers were unaffected by dietary treatments. larvae meal can be a suitable alternative protein source for growing broilers and also when used as principal protein contributor to the diet.

Protein replacement in practical diets altered gut allochthonous bacteria of cultured cyprinid species with different food habits

As the cheap and sustainable recycled natural resources, intestinal casing meal (ICM) and yeast of monosodium glutamate (YMG) might be alternative protein sources in aquaculture diets. To determine the effects of dietary ICM and YMG on fish growth, feed utilization and gut allochthonous bacteria, 8-week feeding trials were conducted with blunt snout bream (Megalobrama amblycephala, MA; a typical herbivorous cultured fish species in China), black carp (Mylopharyngodon piceus, MP; a typical carnivorous species in China) and gibel carp (Carassius gibelio, CG; a typical omnivorous species in China) with partial or complete replacement of soybean meal by ICM or ICM and YMG mixture. The results indicated that ICM and YMG incorporation had positive effects (P value >0.05) on weight gain and feed conversion rate. The complete replacement altered the gut allochthonous microbiota of gibel carp and black carp (Cs < 0.70). Proteobacteria, Fusobacteria, Firmicutes and uncultured bacteria were predominant bacteria in these fish intestine by DGGE detection. Compared with the control diet group with SBM, intestinal allochthonous bacteria of fishes fed with ICM and YMG were significantly stimulated, such as proteobacterium clone (EF707282.1), Cetobacterium somerae (AB353124) and some Bacillus subtilis dependent on fish species. Based on these results, ICM and YMG have the potential to replace SBM in aqua feed, and altered gut allochthonous bacteria variedly as fish food habitats.