The accuracy of the intaglio surface of maxillary complete dentures produced with a variety of digital manufacturing systems have been comprehensively investigated, however, not for multiple systems in the same study. This in vitro study endeavors to measure and compare the accuracy of the intaglio surface of maxillary complete denture bases constructed with different photopolymerization-based 3D printing and CNC milling systems. Two subtractive manufacturing machines and five additive manufacturing machines were used to manufacture a maxillary denture bases (n=10) for analysis. The samples were stored in artificial saliva at 37°C for 7 days to mimic intraoral use before they were air dried for 5 min, and then scanned with an E3 dental laboratory scanner to generate STL files. A 3D metrology software program was used to analyze the data against the original CAD design. Statistical differences were determined with 1-way analysis of variance (ANOVA) and the Kruskal Wallis test (α=.05). Color deviation heat maps were used to interpret areas of clinical significance. The results indicated that the subtractive manufacturing method delivers the truest and most precise intaglio surface of maxillary complete denture bases. The additive manufacturing technique delivers less true and less precise results with inconsistency observed between the different systems as opposed to the subtractive manufacturing groups which showed no significant differences. When manufacturing a complete maxillary denture base subtractive manufacturing will deliver the most consistent and true results.