Complete Cycle Research Articles

The Bowland Shale Formation in the Blacon Basin: syngenetic processes, stacking patterns and heat productivity

Abstract We conducted a high-resolution multi-disciplinary analysis of two core sections in the borehole Ellesmere Port-1, Cheshire, UK. Biostratigraphic analysis indicates that the core sections are Kinderscoutian and late Arnsbergian–Chokierian in age, respectively. Both cores are assigned to the Bowland Shale Formation (Holywell Shale). Coupled core scan and discrete geochemical analysis enables interpretation of syngenetic processes at a high stratigraphic resolution. Both cores exhibit the classic cyclicity of limestones, calcareous to non-calcareous mudstones and siltstones, interpreted to represent sediment deposition during fourth-order sea-level fluctuation. Machine learning of the well log data coupled to the core scan data enabled prediction of the key lithofacies through the entire Bowland Shale interval in Ellesmere Port-1. The machine predictions show that the Bowland Shale is interfingered with three turbiditic leaves of the Cefn-y-Fedw Sandstone Formation and contains at least 12 complete fourth-order cycles. The Bowland Shale exhibits high radiogenic heat productivity in comparison with other sedimentary rocks, due primarily to relative U enrichment under intermittently euxinic conditions. Thermal modelling, however, shows that the radiogenic heat productivity of the Bowland Shale contributes a negligible source of additional heat at the scale of hundreds of metres.

Tucidinostat Plus Exemestane as a Neoadjuvant in Early-Stage, Hormone Receptor-Positive, Human Epidermal Growth Factor Receptor 2-Negative Breast Cancer.

To assess the efficacy and safety of tucidinostat plus exemestane as a neoadjuvant strategy in early-stage breast cancer. This prospective, open-label, single-arm phase II trial enrolled patients with stage II-III breast cancer with hormone receptor-positive and human epidermal growth factor receptor 2 (HER2)-negative. Eligible patients received tucidinostat plus exemestane, and then breast-conserving surgery (BCS) or modified radical mastectomy. Among 20 enrolled patients, 3 of them achieved preoperative endocrine prognostic index (PEPI) score of 0. Additionally, complete cell cycle arrest was observed in 7, radiologic objective response rate in 10, and disease control rate in 20 patients, pathological complete response in 1 patient, and 5 patients performed BCS. Ki67 suppression from baseline to surgery was observed in 17 of patients, with the Ki67 change ratio of -73.5%. Treatment-emergent adverse event included neutropenia, leukopenia, thrombocytopenia, lymphopenia, hypoalbuminemia, aspartate aminotransferase elevation, glutamyl transpeptidase elevation, anemia, and alanine aminotransferase elevation. Despite the rate of PEPI score 0 was not high, tucidinostat plus exemestane as a neoadjuvant therapy might be well tolerated and showed promising clinical responses in patients with early hormone receptor-positive, HER2-negative breast cancer. To clarify the safety and efficacy of this strategy, further investigation is warranted. ChiCTR2100046678.

Impact of groundwater representation on heat events in regional climate simulations over Europe

Abstract. The representation of groundwater is simplified in most regional climate models (RCMs), potentially leading to biases in the simulations. This study introduces a unique dataset from the regional Terrestrial Systems Modelling Platform (TSMP) driven by the Max Planck Institute Earth System Model at Low Resolution (MPI-ESM-LR) boundary conditions in the context of dynamical downscaling of global climate models (GCMs) for climate change studies. TSMP explicitly simulates full 3D soil and groundwater dynamics together with overland flow, including complete water and energy cycles from the bedrock to the top of the atmosphere. By comparing the statistics of heat events, i.e., a series of consecutive days with a near-surface temperature exceeding the 90th percentile of the reference period, from TSMP and those from GCM–RCM simulations with simplified groundwater dynamics from the COordinated Regional Climate Downscaling EXperiment (CORDEX) for the European domain, we aim to improve the understanding of how groundwater representation affects heat events in Europe. The analysis was carried out using RCM outputs for the summer seasons of 1976–2005 relative to the reference period of 1961–1990. While our results show that TSMP simulates heat events consistently with the CORDEX ensemble, there are some systematic differences that we attribute to the more realistic representation of groundwater in TSMP. Compared to the CORDEX ensemble, TSMP simulates fewer hot days (i.e., days with a near-surface temperature exceeding the 90th percentile of the reference period) and lower interannual variability and decadal change in the number of hot days on average over Europe. TSMP systematically simulates fewer heat waves (i.e., heat events lasting 6 d or more) compared to the CORDEX ensemble; moreover, they are shorter and less intense. The Iberian Peninsula is particularly sensitive with respect to groundwater. Therefore, incorporating an explicit 3D groundwater representation in RCMs may be a key in reducing biases in simulated duration, intensity, and frequency of heat waves in Europe. The results highlight the importance of hydrological processes for the long-term regional climate simulations and provide indications of possible potential implications for climate change projections.

Improvement of topside ionosphere and plasmasphere estimation in ionospheric simulation system

This work is based on the results for a complete solar cycle of 11 years (2011–2021) from the ionospheric simulation system used in the Brazilian Space Weather program. The electron density profiles generated were integrated in height and compared to the International GNSS Service (IGS) vertical total electron content (vTEC) data. Experiments have shown an inadequate electron concentration decay with height which led to an overestimation in TEC values. We propose an improvement for the topside ionosphere and plasmasphere modeling in the system, considering recent advances in empirical modeling through observational data. The idea is to combine bottomside ionosphere estimates from the physical-based core model with NeQuick analytical formulation for ionospheric profile above hmF2. Our approach also calculates the ionospheric parameters for topside modeling using bottomside estimates. The ionospheric density profile decay obtained with the proposed approach was smoother and sharper, reaching a reduction of approximately 1 to 2 orders of magnitude of electron concentration in plasmasphere. It was observed a coherent reduction of high altitudes ionosphere/plasmasphere contribution to TEC. Also, the TEC values obtained using this hybrid solution were much closer to the International GNSS Service (IGS) data for the considered dataset, with root mean squared error (RMSE) of only 2.64 TECU for the entire period.

Design, development and characterization of Blumlein Pulse Forming Line based pulsed power system for High Power Microwave source research

This paper describes a complete cycle of designing, developing, and characterising a Pulsed Power System (PPS) and generating microwaves using the relativistic source. For low impedance relativistic High Power Microwave (HPM) sources, Blumlein Pulse Forming Line (BPFL) based high voltage pulsed power systems are the most suited. An HPM source such as a virtual cathode oscillator (VIRCATOR) operating in nanosecond durations consists of a primary voltage source that charges a Marx pulse generator’s capacitor bank over a long time. The requirements of a diode load are met by compressing the output pulse width of a Marx generator by a BPFL. This article describes the novel design, construction and characterization of a circulating De-Ionized (DI) water insulated BPFL pulsed power system. A novel approach of insulating gas filled Marx generator of this class/rating is attempted. A pulse power system consisting of high pressure gas insulated Marx generator and circulating DI water-based BPFL was developed and experimented with the resistive load as well as HPM source. The PSPICE simulation of the Blumlein circuit for various load conditions is described. Criticalities in the design of end bushings are elaborated along with the development and testing of a compact BPFL-based pulsed power system.

Implementation of Building Information Modeling Technologies in Wood Construction: A Review of the State of the Art from a Multidisciplinary Approach

This research raises questions about the possibilities and options of using the BIM methodology associated with software for the wood design and construction of structure modeling along an asset’s cycle life. Likewise, several academic and research initiatives are reviewed. In this sense, this paper aims to establish an appropriate link between two agendas that the architecture, engineering, and construction (AEC) industry, academia, and governments normally handle separately. By conducting several literature reviews (book, journals, and congresses) and extensive software tests (BIM software: Revit v2023, Archicad v27, Tekla, and wood plug-ins: AGACAD, Archiframe, Timber Framing 2015, WoodStud Frame, etc.), the state-of-the-art was assessed in both fields, and several cases linking BIM and wood are shown in detail and discussed. Various theoretical samples are modelled and shown, and the advantages and disadvantages of each technique and stage are explained. On the other hand, although wood construction has been most common for hundreds of years, this is not the case of BIM software developments associated with this materiality. Furthermore, since the appearance of materials such as steel and reinforced concrete, all software developments have focused on these materials, leaving aside the possibility of developing applications for use in wood projects. According to that previously discussed, it can be concluded that BIM for wood has been used more frequently in academia, that both fields have several common processes, and, in many cases, that only a few BIM-wood tools have been used, thus disregarding the high potential and high level of benefits that result with the application of these methodologies for the complete building life cycle (design, construction, and operation).

Photoredox matching of earth-abundant photosensitizers with hydrogen evolving catalysts by first-principles predictions.

Photoredox properties of several earth-abundant light-harvesting transition metal complexes in combination with cobalt-based proton reduction catalysts have been investigated computationally to assess the fundamental viability of different photocatalytic systems of current experimental interest. Density functional theory (DFT) and time-dependent DFT (TD-DFT) calculations using several GGA (BP86, BLYP), hybrid-GGA (B3LYP, B3LYP*), hybrid meta-GGA (M06, TPSSh), and range-separated hybrid (ωB97X, CAM-B3LYP) functionals were used to calculate relevant ground and excited state reduction potentials for photosensitizers, catalysts, and sacrificial electron donors. Linear energy correction factors for the DFT/TD-DFT results that provide the best agreement with available experimental reference results were determined in order to provide more accurate predictions. Among the selection of functionals, the B3LYP* and TPSSh sets of correction parameters were determined to give the best redox potentials and excited states energies, ΔEexc, with errors of ∼0.2eV. Linear corrections for both reduction and oxidation processes significantly improve the predictions for all the redox pairs. In particular, for TPSSh and B3LYP*, the calculated errors decrease by more than 0.5V against experimental values for catalyst reduction potentials, photosensitizer oxidation potentials, and electron donor oxidation potentials. Energy-corrected TPSSh results were finally used to predict the energetics of complete photocatalytic cycles for the light-driven activation of selected proton reduction cobalt catalysts. These predictions demonstrate the broader usefulness of the adopted approach to systematically predict full photocycle behavior for first-row transition metal photosensitizer-catalyst combinations more broadly.

Lineage Switching from Acute Myeloid Leukemia associated with Systemic Mastocytosis to B-Acute Lymphoblastic Leukemia: A Diagnostic Dilemma

Relapse acute leukemia is defined as the reappearance of more than 5% blast in the bone marrow. In most instances, blast expresses the specific lineage (myeloid or lymphoid), similar to a diagnosis. However, it rarely converts to a different lineage, either myeloid shifting to lymphoid or vice versa, during relapse. Thus, it is labelled as switching lineage acute leukemia, after excluding the criteria for mixed phenotypic acute leukemia, which can be a challenge to diagnose. We describe a 24-year-old gentleman with a known case of acute myeloid leukemia associated with systemic mastocytosis (SM); eight months later and after two complete cycles of chemotherapy, relapse occurred with a switched lineage to B-acute lymphoblastic leukemia. The laboratory investigation approaches and the challenges in the diagnosis are also discussed in this case report

Observations key to understanding solar cycles: a review

A paradigm shift is taking place in the conception of solar cycles. In the previous conception, the changing numbers of sunspots over intervals of 9–14 years have been regarded as the fundamental solar cycle although two average 11-year cycles were necessary to account for the complete magnetic cycle. In the revised picture, sunspots are a phase in the middle of two 22-year overlapping solar cycles that operate continuously with clock-like precision. More than 20 researchers have contributed to the initial research articles from 2014 through 2021 which are dramatically altering the perception of solar cycles. The two 22-year cycles overlap in time by 11 years. This overlap is coincidentally the same average duration as the sunspot phase in each 22-year cycle. This coincidence and the relative lack of knowledge of the large numbers of small active regions without sunspots is what led to the previous paradigm in which the 11-year sunspot phases were misinterpreted as a single fundamental solar cycle. The combination of the two 22-year solar cycles, with their large numbers of short-lived active regions and ephemeral active regions are now understood to be the fundamental cycle with the proposed name “The Hale Solar Cycle.” The two 22-year solar cycles each occupy separate but adjacent bands in latitude. The orientations of the majority of bipolar magnetic regions in the two adjacent bands differ from each other by ∼180°. Both bands continuously drift from higher to lower latitudes as has been known for sunspot cycles. However, the polarity reversal occurs at the start of each 22-year cycle and at higher latitudes than it does for the sunspot cycles. This paradigm shift in the concept of solar cycles has resulted in major reconsiderations of additional topics on solar cycles in this review. These are 1) the large role of ephemeral active regions in the origin of solar cycles, 2) the depth of the origin of active regions and sunspots, 3) the mechanisms of how areas of unipolar magnetic network migrate to the solar poles every 11 years, and 4) the nature of the polarity reversal in alternate 22-year cycles rather than 11-year cycles.

Majority of human circulating IgG plasmablasts stop blasting in a cell-free pro-survival culture

Following infection or vaccination, early-minted antibody secreting cells (ASC) or plasmablasts appear in circulation transiently, and a small fraction migrates to the spleen or bone marrow (BM) to mature into long-lived plasma cells (LLPC). While LLPC, by definition, are quiescent or non-dividing, the majority of blood ASC are thought to be “blasting” or proliferative. In this study, we find > 95% nascent blood ASC in culture express Ki-67 but only 6–12% incorporate BrdU after 4 h or 24 h labeling. In contrast, < 5% BM LLPC in culture are Ki-67+ with no BrdU uptake. Due to limitations of traditional flow cytometry, we utilized a novel optofluidic technology to evaluate cell division with simultaneous functional IgG secretion. We find 11% early-minted blood ASC undergo division, and none of the terminally differentiated BM LLPC (CD19−CD38hiCD138+) divide during the 7–21 days in culture. While BM LLPC undergo complete cell cycle arrest, the process of differentiation into an ASC or plasmablasts also discourages entry into S phase. Since the majority of Ki-67+ nascent blood ASC have exited cell cycle and are no longer actively “blasting”, the term “plasmablast”, which traditionally refers to an ASC that still has the capacity to divide, may probably be a misnomer.

Scale resolving simulations of the effect of glottis motion and the laryngeal jet on flow dynamics during respiration

Background and ObjectiveThe movement of the respiratory walls has a significant impact on airflow through the respiratory tract. The majority of computational fluid dynamics (CFD) studies assume a static geometry which may not provide a realistic flow field. Furthermore, many studies use Reynolds Averaged Navier-Stokes (RANS) turbulence models that do not resolve turbulence structure. Combining the application of advanced scale-resolving turbulence models with moving respiratory walls using CFD will provide detailed insights into respiratory flow structures. MethodsThis study simulated a complete breathing cycle involving inhalation and exhalation in a nasal cavity to trachea geometry that incorporated moving glottis walls. A second breathing cycle was simulated with static glottis walls for comparison. A recently developed hybrid RANS-LES turbulence model, the Stress-Blended Eddy Simulation (SBES), was incorporated to resolve turbulent flow structures in fine detail for both transient simulations. Transient results were compared with steady-state RANS simulations for the same respiratory geometry. ResultsGlottis motion caused substantial effects on flow structure through the complete breathing cycle. Significant flow structure and velocity variations were observed due to glottal motion, primarily in the larynx and trachea. Resolved turbulence structures using SBES showed an intense mixing section in the glottis region during inhalation and in the nasopharynx during expiration, which was not present in the RANS simulations. ConclusionTransient simulations of a realistic breathing cycle uncovered flow structures absent in simulations with a constant flow rate. Furthermore, the incorporation of glottis motion impacted airflow characteristics that suggest rigid respiratory walls do not accurately describe respiratory flow. Future research in respiratory airflow should be conducted using transient scale-resolving models in conjunction with moving respiratory walls to capture flow structures in detail.

The early-tenure salesperson: sales effort and sales growth during the ramp-up period

The challenges encapsulated in the ‘War for Sales Talent’ are symbolic of the struggles inherent in the recruitment, nurturing, and retention of sales professionals. While extant research provides valuable insights into facets like hiring practices, selection criteria, coaching, and training strategies, a noticeable void persists in guiding salespeople as they navigate beyond the initial ramp-up period in a new role. By interconnecting survey responses from a cohort of 200 service salespeople with 18–24 months of tenure, this investigation brings to light the facets of effort—encompassing prospecting, conversion, retention, and growth—that exert an influence on the change in year-over-year revenue following a salesperson’s first complete sales cycle post-onboarding. The results underscore the significance of conversion and growth efforts, revealing their robust impact on the net change in sales revenue. In challenging existing paradigms, this study proposes a conceptual shift in understanding sales effort. Rather than adhering to the prevailing unidimensional viewpoint, the findings advocate for recognizing sales effort as a multi-dimensional construct with competing demands. This nuanced perspective contributes to a more comprehensive understanding of the factors influencing revenue growth for salespeople transitioning from the ramp-up period toward a sustainable, growth-oriented trajectory.

Stochastic model of vesicular stomatitis virus replication reveals mutational effects on virion production.

We present the first complete stochastic model of vesicular stomatitis virus (VSV) intracellular replication. Previous models developed to capture VSV's intracellular replication have either been ODE-based or have not represented the complete replicative cycle, limiting our ability to understand the impact of the stochastic nature of early cellular infections on virion production between cells and how these dynamics change in response to mutations. Our model accurately predicts changes in mean virion production in gene-shuffled VSV variants and can capture the distribution of the number of viruses produced. This model has allowed us to enhance our understanding of intercellular variability in virion production, which appears to be influenced by the duration of the early phase of infection, and variation between variants, arising from balancing the time the genome spends in the active state, the speed of incorporating new genomes into virions, and the production of viral components. Being a stochastic model, we can also assess other effects of mutations beyond just the mean number of virions produced, including the probability of aborted infections and the standard deviation of the number of virions produced. Our model provides a biologically interpretable framework for studying the stochastic nature of VSV replication, shedding light on the mechanisms underlying variation in virion production. In the future, this model could enable the design of more complex viral phenotypes when attenuating VSV, moving beyond solely considering the mean number of virions produced.

The Tengmalm's owl Aegolius funereus (Aves, Strigidae) as the definitive host of Sarcocystis funereus sp. nov. (Apicomplexa).

Owls have been reported as definitive hosts, whereas wild small mammals (naturally and experimentally) as intermediate hosts of several species of Sarcocystis. Recently, dead fledglings were found infected by an unnamed species of Sarcocystis since its intermediate host was unknown. After collecting additional samples of owls and wild small mammals, the present study focused on elucidating the identity, potential intermediate host, and complete life cycle of the found Sarcocystis through experimentally infected rodents. The developmental stages' morphological and molecular characterizations (28S rRNA gene, ITS1 region) are presented herein. In total, 21 Tengmalm's owl carcasses (15 nestlings, 5 fledglings, and 1 adult male) were collected in Kauhava (west-central Finland) and parasitologically examined by wet mounts. Intestinal mucosa scrapings were used to isolate oocysts/sporocysts and employed for experimental infections in dexamethasone-immunosuppressed BALB/cOlaHsd mice. Additionally, sarcocysts were searched in the skeletal muscle of 95 samples from seven wild small mammal species. All these developmental stages were molecularly characterized by the 28S rRNA gene and ITS1 region. Experimental infections were carried out by using immunosuppressed female 8-week-old BALB/cOlaHsd mice, divided into three groups: (1) water with 15 μg/mL of dexamethasone, (2) water with 30 μg/mL of dexamethasone, (3) no dexamethasone treatment. Each group consisted of four individuals. In each group, two mice were infected with 1,000 sporocysts each, and the remaining two with 10,000 sporocysts each. All mice were euthanized on specific days post-infection. The intestinal mucosa of 11 nestlings and 5 fledglings of the Tengmalm's owl were positive for Sarcocystis funereus sp. nov. The adult male owl and all owls' breast and heart muscles were negative for Sarcocystis. Two dexamethasone-immunosuppressed BALB/cOlaHsd mice (group 2) were positive to S. funereus sp. nov. in diaphragm and leg muscles after 22- and 24-day post-infection. Some sarcocysts were found in the wild small mammals. Molecular identification at 28S rRNA revealed sequences from naturally infected Tengmalm's owls, as well as sarcocysts of dexamethasone-immunosuppressed BALB/cOlaHsd mice were 99.87-100% similar to Sarcocystis sp. isolate Af1 previously found in the Tengmalm's owl. At the ITS1 region, the S. funereus sp. nov. isolates Af2 haplotype B and Af3 haplotype A were 98.77-100% identical to Sarcocystis sp. isolate Af1. The sequences from sarcocysts of naturally infected wild small mammals were 75.23-90.30% similar at ITS1 region to those of S. funereus sp. nov. The morphological and molecular characterizations and phylogenetic placement of S. funereus sp. nov. are presented here for the first time and support the erection of the new species.

Towards a more sustainable balance between optimal live birth rate and supernumerary embryos in ART treatments.

To assess the relation between number of inseminated oocytes and cumulative live birth rate (CLBR) in order to provide guidance for limiting the number of surplus blastocysts. The study was a retrospective, single-center cohort analysis of 1223 ART complete cycles. Cycles were stratified according to female age (≤ 34, 35-38, and 39-42 years) and number of inseminated oocytes (1-5, 6-10, and > 10). Inclusion criteria were indication for IVF/ICSI with own spermatozoa and blastocyst culture up to day 6 of all embryos. In patients younger than 35 years, insemination of more than ten oocytes produced an increase in overall blastocyst number, CLBR (40.3%, 54.3%, and 75.8%, respectively, for each oocyte group) and surplus embryo rate (12.9%, 27.8%, and 49.7% of cases for each group). Instead, in the middle age group, the use of more than ten oocytes was solely associated with an increase in the rate of surplus embryos (1.25%, 21.33%, and 28.68% of cases after stratification for oocyte number). In older patients, neither CLBR (9.1%, 23.9%, and 24.7%, respectively) nor rate of surplus embryos (2.0%, 7.1%, and 13.4% of cases for each group) were higher in cycles with more than ten inseminated oocytes. In women up to 38 years, sustainable CLBR are achieved while limiting the number of inseminated oocytes and the resulting blastocysts remaining unused. Based on this notion, novel treatment strategies could pursue high outcome rates, while alleviating the problems derived from surplus stored embryos.

Understanding the Tail Current Behavior of Electroactive Biofilms Realizes the Rapid Measurement of Biochemical Oxygen Demand.

The use of microbial electrochemical sensors, with electroactive biofilms (EABs) as sensing elements, is a promising strategy to timely measure the biochemical oxygen demand (BOD) of wastewater. However, accumulation of Coulombic yield over a complete degradation cycle is time-consuming. Therefore, understanding the correlation between current output and EAB metabolism is urgently needed. Here, we recognized a tail stage (TS) on a current-time curve according to current increase rate─a period with the least electron harvesting efficiency. EAB adopted a series of metabolic compensation strategies, including slow metabolism of residual BOD, suspended growth, reduced cell activity, and consumption of carbon storage polymers, to cope with substrate deficiency in TS. The supplementary electrons provided by the decomposition of glycogen and fatty acid polymers increased the Coulombic efficiencies of TS to >100%. The tail current produced by spontaneous metabolic compensation showed a trend of convergent exponential decay, independent of BOD concentration. Therefore, we proposed the TS prediction model (TSPM) to predict Coulombic yield, which shortened BOD measurement time by 96% (to ∼0.5 h) with deviation <4 mg/L when using real domestic wastewater. Our findings on current output in TS give insights into bacterial substrate storage and consumption, as well as regulation in substrate-deficient environment, and provide a basis for developing BOD sensors.

A Pseudoinversion-Free Method for Weight Updating in Broad Learning System.

Neural networks have evolved into one of the most critical tools in the field of artificial intelligence. As a kind of shallow feedforward neural network, the broad learning system (BLS) uses a training process based on random and pseudoinverse methods, and it does not need to go through a complete training cycle to obtain new parameters when adding nodes. Instead, it performs rapid update iterations on the basis of existing parameters through a series of dynamic update algorithms, which enables BLS to combine high efficiency and accuracy flexibly. The training strategy of BLS is completely different from the existing mainstream neural network training strategy based on the gradient descent algorithm, and the superiority of the former has been proven in many experiments. This article applies an ingenious method of pseudoinversion to the weight updating process in BLS and employs it as an alternative strategy for the dynamic update algorithms in the original BLS. Theoretical analyses and numerical experiments demonstrate the efficiency and effectiveness of BLS aided with this method. The research presented in this article can be regarded as an extended study of the BLS theory, providing an innovative idea and direction for future research on BLS.

Well-being in healthy Icelandic women varies with extreme seasonality in ambient light.

Seasonal variation in photoperiod may affect psychosocial and physical well-being in healthy persons. We tested this hypothesis in healthy pre-menopausal women, without a history of mood disorders, living year-round in Reykjavik, Iceland (64.1°N). Participants reported daily self-assessments of well-being throughout a complete ovulatory menstrual cycle in summer and/or winter (70% participated in both seasons). Scores for mood, cognitive acuity, social support, physical health and a composite of these four indicators were each significantly higher in summer than in winter (linear mixed effects models: p < .001 for each model); tiredness did not differ by season. The effect of season was not significantly changed by inclusion of body mass index and/or age as covariates. Some prior studies have been hampered by sparse time sampling, inattention to covariates and/or relying on recalled data. This is to our knowledge the first investigation to test the study hypothesis with daily real-time data spanning complete ovulatory menstrual cycles in each of two seasons. This dense sampling has revealed modest seasonal variation in well-being in healthy women. Daylength (sunlight exposure) is likely a major, but not necessarily sole, factor in these seasonal differences in well-being; temperature is likely less important given Iceland's relatively moderate (for its high latitude) seasonal temperature swings.

Metabolic shifts in glycogen-accumulating organisms: Exploring carbon and phosphorus dynamics under two distinct phosphorus limitation modes

Glycogen-accumulating organisms (GAOs) are recognized as competitors of phosphorus-accumulating organisms (PAOs) in enhanced biological phosphorus removal (EBPR) systems. However, different types of GAOs exhibit unique metabolic traits in different environments. To characterize the metabolic activity of GAOs, two distinct modes were constructed. Long-term operation with the mode of anaerobic post-drainage demonstrated the successful establishment of a rapid and enduring GAOs system dominated by Candidatus Contendobacter community. Acetate was completely consumed within 60 min of the anaerobic phase. Concurrently, glycogen levels decreased by 0.062 g Glycogen/g SS, while intracellular carbon storage of poly-hydroxybutyrate (PHB) and poly-hydroxyvalerate (PHV) increased by 0.027 g PHB/g SS and 0.007 g PHV/g SS, respectively. Various concentrations of phosphorus loads illustrated the resistance of the system to high phosphorus loads, while maintaining glycogen accumulating metabolism (GAM). Under complete phosphorus limitation, no correlation between glycogen and acetate was observed during the initial 140 d. The addition of 1 mg/L phosphate to the influent preserved the typical GAM characteristics. Moreover, the pathway involved in glycogen conversion to trehalose may be pivotal in enhancing anaerobic glycogen degradation. The complete tricarboxylic acid cycle ensured efficient energy production, thereby sustaining the foundational metabolism. Notably, sucC and mdh were critical regulators of energy flux. This study provides a distinctive metabolic profile of a robust and resilient GAOs model with Ca. Contendobacter.

Well-controlled preparation of WO3-x/WSe2 sponges by electric field-assisted laser-induced plasma deposition for hydrovoltaic

Hydrovoltaic, which converts the energy of water into electrical energy, is a regenerative energy harvesting method that utilizes the earth's sufficient water resources. Electricity production relies on water molecules interaction with functional materials. Strategies like surface modification, structure engineering, and ion doping can be used to increase output performance. We present an electric field-assisted laser-induced plasma deposition method to prepare WO3-x/WSe2 sponges for water-induced electricity generation. By adjusting the electric field strength, we are able to control the porosity, Se vacancies, and O doping of the sponges. These modifications directly impact the performance of water-induced electricity generation by altering the streaming potential and ion diffusion gradient of sponge structures. The prepared WO3-x/WSe2 sponges can participate in the complete water cycle to achieve the energy harvesting of different forms of water, obtaining droplet-induced voltages of 65.4 mV and moisture-induced voltages of 207.1 mV. This overcomes the limitation of single-stimulation water-induced electricity systems that require specific conditions. When exposed to Sunlight, the photosensitive properties of WO3-x and WSe2 can lead to a current up to 7 times higher than that produced in darkness. Furthermore, this research can be expanded to develop other two-dimensional materials and be utilized in various applications, such as the self-powering of flexible and wearable electronic devices.