BackgroundThe final goal for treatment of congenital heart diseases (CHD) is to resume not only the normal heart structure but also physiology. The present study evaluates the proteomics changes before and after surgery in CHD with tetralogy of Fallot (TOF) or ventricular septal defect (VSD).MethodsDifferential plasma proteins by using two‐dimensional gel electrophoresis and mass spectrometry followed by ELISA validation in new cohort were analyzed in patients with TOF (n=82) or VSD (n=82) compared to normal controls (n=97) preoperatively and 6‐month postoperatively.ResultsA total of 473 protein spots preoperatively and 515 postoperatively including those significantly (p<0.01) downregulated or upregulated were identified. The ELISA validation for chosen proteins demonstrated that in VSD patients, postoperative alpha‐1‐acid glycoprotein 2 and serum amyloid p‐component were completely recovered (p>0.05, compared to control) and complement component C3c was partially recovered (p<0.05, compared to control) towards normal after surgery. In TOF patients, postoperative fibrinogen gamma chain was completely and gelsolin was partially recovered.ConclusionsWe have for the first time demonstrated that CHD repair surgery not only corrects the structure malformation but also resumes the normality of altered proteins that may be developed as biomarkers. Identification of the recovered or unchanged proteins may be used for evaluation of the surgical results and for facilitating the personalized management for CHD.Support or Funding InformationSupported by grants from the National Natural Science Foundation of China (81170148 & 81641017), Zhejiang Provincial Natural Science Foundation (LY15H020008), & Tianjin Binhai Key Platform for Creative Research Program (2012‐BK110004), Binhai New Area Health Bureau (2012BWKZ008, 2016BWKY007, 2016BWKZ003).This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.