Aim: This in vitro study was done to compare the shaping ability of ProTaper, K3, and Hero Shapers in clear resin blocks with simulated root canals using computer image software analysis. Materials and Methods: 45 clear resin blocks with simulated root canals were divided into three groups of 15 each. Preinstrumentation digitalized images of the blocks were made and stored. Each group was instrumented with ProTaper, K3, and Hero Shapers, respectively. Postinstrumentation images of the blocks were made after injecting a contrast medium to improve the outlines of the root canals. Pre- and post-instrumentation images were magnified, superimposed, and assessed using computer image analysis program (pattern matching analysis). Material removal was measured at nine points in the inner wall and outer wall ending 1 cm from the apical terminus. Statistical Analysis: Student's t-test and one-way analysis of variance were used to compare the overall change in the width in the mesial view by using ProTaper, K3, and Hero Shapers. Results: ProTaper showed more change in width, followed by K3 and Hero Shapers. Conclusion: ProTaper and K3 showed significant removal of material in the outer wall of the coronal and apical 3 rd , followed by inner wall of the middle 3 rd . Hero Shapers showed significant removal of material in the outer wall of coronal 3 rd , with less significant changes in the middle 3 rd and even material removal in the apical 3 rd .