Comparison Of Liquid-based Cytology Research Articles

Comparison of Liquid-Based Cytology and Conventional Papanicolaou Smear for Cervical Cancer Screening: An Experience From Pakistan.

IntroductionAfter the introduction of cervical cancer screening program with cervical cytology, a marked decline in deaths secondary to cervical cancer was observed in developed countries. Two methods are used for cervical cytology. The first one is the conventional Papanicolaou (PAP) and the second one is liquid-based cytology (LBC). Although various studies in western countries established the role of LBC in cervical cancer screening, no large-scale study was conducted in our population to compare the two techniques for cervical cancer screening. Therefore, in this study, we compared the diagnostic utility of these two techniques for detecting cervical epithelial lesions.MethodsA total of 3,929 patients, who presented to the Gynecology Clinic, Liaquat National Hospital, for cervical cancer screening from January 2015 until December 2019, over a period of five years, were included in the study. A total of 1,503 specimens were prepared by LBC, and 2,426 specimens were prepared by a conventional PAP smear method. All smears were interpreted using the Bethesda System of Reporting Cytopathology.ResultsThe mean age of the patients was 39.46±11.14 years. For cytological evaluation, 98.7% of specimens were adequate. The inadequacy rate was 1.3% for conventional PAP smear and 1.2% for LBC. While 97.2% of specimens were reported as negative for intraepithelial lesion or malignancy, 1.1% of specimens showed squamous epithelial lesions. There was a significant difference in the detection rate of squamous epithelial lesions using the two techniques. The detection rate of squamous intraepithelial lesions using LBC was 2.1%, which was higher than that of the conventional PAP smear (0.6%). The detection rates of glandular lesions using LBC and conventional PAP smear were 0.5% and 0.2%, respectively.ConclusionWe found a higher disease detection rate of squamous epithelial lesions using LBC compared to conventional PAP smear. Therefore, we recommend a widespread use of LBC for mass cervical cancer screening in our population.

Fine needle aspiration cytology of cervical lymph nodes: Comparison of liquid based cytology (SurePath) and conventional preparation.

Fine needle aspiration cytology (FNAC) is the first diagnostic step in patient with cervical lymphadenopathy because of its simplicity, safety and early availability of the results. Liquid-based cytology (LBC) is an alternative processing method which is used for both gynecological and nongynecological samples. Literature reviewed show few studies comparing LBC with conventional preparation (CP). The present study was undertaken to evaluate the efficacy of LBC and comparison of LBC and CP in cervical lymphadenopathy. In this prospective study, a total of 75 cases of FNAC with cervical lymphadenopathy were included. The first pass was used for CP followed by LBC with the use of SurePath (SP) technique. Both the smears were compared for cellularity, background containing blood, cell debris, lymphoglandular bodies, stromal fragments, cytoarchitectural pattern, etc., by semiquantitative scoring system. There was no statistical difference in the cellularity, cell architecture, and monolayer cells (P > .05). On the basis of background containing blood, cell debris, lympho-glandular bodies, stromal fragments (P < .001), nuclear, and cytoplasmic details (P < .05), LBC was found to be superior to CP. LBC is a relatively simple technique and superior to CP in respect of better nuclear and cytoplasmic details with loss of background blood and debris. It has a diagnostic accuracy equivalent to that of CP. However, use of both LBC and CP can result in better diagnostic accuracy.

A comparison of liquid-based cytology and conventional smears in fine needle aspiration cytology of thyroid lesions: Diagnostic efficacy and pitfalls

Introduction: Fine needle aspiration cytology is an unrivalled method of preoperative triage of thyroid lesions. The use of liquid-based cytology (LBC) is now extended to nongynecological samples due to the advantages of lesser screening time, clearer background, and potential use of leftover material for ancillary investigations. Aims and Objectives: The aim of the study is cytomorphological comparison of LBC and conventional method and cytohistopathological correlation in surgically resected cases. Materials and Methods: Fine needle aspiration was done in 100 patients. Conventional smears were prepared followed by LBC from the remaining material. The smears from both the methods were scored semiquantatively and were compared cytomorphologically. Pearson's Chi-square test and fisher's exact test were used for P value calculation using SPSS software. A P Results: LBC had more unsatisfactory as well as suspicious for malignancy cases. The cells in LBC were in three-dimensional clusters and singly scattered with loss of cellular architecture. The nuclei were shrunkun and hyperchromatic with spilling of cytoplasm. The background colloid, blood as well as lymphocytes were mostly washed off. Immunocytochemisrty results were not affected by the LBC procedural steps. Conclusion: It was very difficult to come to a conclusive diagnosis with the help of LBC alone. It can be useful for ancillary investigations like immunocytochemistry as in this study. Thus, it can be used as a supplement to conventional method rather than replacing it.

Comparison of liquid-based cytology with conventional smear cytology for EUS-guided FNA of solid pancreatic masses: a prospective randomized noninferiority study

There are limited data on the efficacy of liquid-based cytology (LBC) for EUS-guided FNA specimens. We aimed to evaluate the diagnostic efficacy of LBC for solid pancreatic neoplasms compared with conventional smears (CSs). In this randomized, crossover, noninferiority trial, we randomly assigned (1:1) patients with suspected pancreatic cancer to the LBC group or the CS group. Aspirates from the first needle pass were processed by one method, aspirates from the second pass by the other method, and specimens from the last pass were processed as core biopsy samples. The primary endpoint was the diagnostic efficacy of each method, with the final diagnosis as the gold standard. A noninferiority margin of-10% was assumed. Of 170 randomized patients, 165 were classified as malignant and 5 as benign. Unsatisfactory samples were less frequent in the LBC group (1.78%) compared with the CS group (5.33%). The diagnostic accuracy, sensitivity, specificity, positive predictive value, and negative predictive value of LBC versus CS were 88.0% versus83.8% (P= .276), 87.7% versus 83.2% (P= .256), 100% versus 100% (P= .999), 100% versus 100% (P= .999), and 16.7% versus 16.1% (P= .953), respectively. A bloody background was significantly more frequent in the CS group (CS, 85.2%; LBC, 1.8%; P< .001), whereas the nuclear features were similar for both groups. The diagnostic usefulness of LBC was comparable with that of CS. The cytomorphologic featuresdid not differ significantly between the 2 methods, and the reduced bloody backgrounds allowed bettervisibility in the LBC method. (Clinical trial registration number: NCT03606148.).

Comparison of Liquid-based Cytology and Conventional Papnicolaou Smear as a Screening Tool in High-risk Females

Comparison of Liquid-based Cytology and Conventional Papnicolaou Smear as a Screening Tool in High-risk Females

Comparison of liquid-based cytology (CellPrepPlus®) and conventional smears in pancreaticobiliary disease

Comparison of liquid-based cytology (CellPrepPlus®) and conventional smears in pancreaticobiliary disease

Comparison of Liquid-Based Cytology (CellPrepPlus®) and Conventional Smears in Pancreaticobiliary Cytology

Comparison of Liquid-Based Cytology (CellPrepPlus®) and Conventional Smears in Pancreaticobiliary Cytology

A comparison of liquid-based cytology with conventional Papanicolaou smears in cervical dysplasia diagnosis.

Background:Due to the high number of women affected by cervical cancer and the importance of an early diagnosis, combined with the frequent incidence of false-negative Papanicolaou (Pap) smear screening results for this disease, several studies have been conducted in recent years in order to find better tests. Liquid-based cytology (LBC) tests, including the liquid-based thin layer method, have demonstrated the highest potential for reducing false-negative cases and improved sample quality. This study aimed to compare the strength of the Pap smear test with fluid cytology and conventional tests in detecting cervical dysplasia.Materials and Methods:This descriptive-analytic study was conducted on 366 women who attended private laboratories for a Pap smear. The Pap smear sampling was conducted simultaneously using two methods: conventional Pap (CP) smear and LBC), from the cervix.Results:The mean age of the participants was 32 ± 8.8 years. Diagnostic results of endocervical cells, epithelial cells, vaginitis cells, and metaplastia were consistent with both conventional and liquid cytology smears, and the kappa coefficient was determined to be significant (P < 0.001). In total, 40.5% of diagnostic cases indicated bacterial inflammation 80.3% of the diagnoses in both methods were P1 and 3.9% of cases diagnosed were P2, the overall diagnostic consistency was 83.9% between the two sampling methods. The inflammation diagnosis was 40.5% and this was consistent in both methods of LBC and CP. There was one case of a false-negative diagnosis in the LBC method and 14 cases in the CP method.Conclusion:Results showed that the LBC may improve the sample's quality and reduce the number of unsatisfactory cases more than with the CP method.

A Comparison of Liquid-Based Cytology with Conventional Cytology in Cervical Cancer Screening

Objective: To clarify the performance of liquid-based cytology (LBC) and conventional methods of preparing cervical specimens for cytological screening. Study Design: We studied 236,511 patients who participated in a population-based cervical cancer screening program conducted in the Niigata prefecture between 2005 and 2008. The percentage of unsatisfactory specimens and the disease detection rate were compared between specimens prepared by LBC and conventional methods. Results: (1) The LBC method demonstrated a significantly lower percentage of unsatisfactory specimens than the conventional method (1.38 and 11.45%, respectively; p < 0.01). (2) Among the initial women, tumor lesions were detected in 0.57% of those examined with the LBC method, which was significantly higher than the positivity rate of those examined with the conventional method (0.25%; p < 0.05). Among the women with repeat screening, disease was detected in 0.08% of those examined with LBC twice, which was significantly lower than the positivity rates for those examined with the conventional method followed by the LBC method (0.11%) or the conventional method twice (0.16%; p < 0.05). Conclusion: The LBC method is significantly more useful than the conventional method in terms of the low adequacy rate and the high detection rate of cancer in cervical cancer screening in a localized area in Japan.

Comparison of Liquid-Based Cytology With Conventional Cytology for Detection of Cervical Cancer Precursors: A Randomized Controlled Trial

The Papanicolaou (Pap) test—the conventional screening method for cervical intraepithelial neoplasia (CIN)—is suboptimal due to high false-negative and false-positive test results due to the poor quality of sampling, preparation problems and errors in detection and interpretation. In a large number of studies, the diagnostic accuracy of an alternative procedure, liquid-based cytology, has been compared with the Pap test with conflicting results. Few of these studies, however, were randomized and none of the randomized trials were well designed. This cluster randomized controlled trial compared liquid-based cytology and the Pap test with respect to testing positivity rates, histologically confirmed detection rates, and positive predictive values (PPVs). The study subjects were women aged 30 to 60 years who were participants in the Dutch cervical screening program. Between 2004 and 2006, 49,222 patients at 122 practices were screened with liquid-based cytology and 40,562 patients at 124 practices were screened with the Pap test. Screen-positive women were followed prospectively for 18 months after initial screening. All medical personnel and others involved with follow-up of screen-positive women were blinded to the cytology screening system used and initial results. Both the intention-to-treat and per-protocol analysis were used. Multivariate logistic regression analysis was used to adjust for possible confounding factors (age, urbanization level, study site, and period). Detection rate ratios of verified cervical abnormalities were calculated for the liquid-based test relative to the Pap test for the primary histological outcome of CIN grades 1+, 2+, and 3+ and carcinoma. The adjusted positive predictive value (PPV) ratios were also calculated. PPV ratios were evaluated at different levels of test positivity and outcome thresholds. The adjusted detection rate ratio for CIN grade 1+ was 1.01, with a 95% confidence interval [CI] of 0.85–1.19); for CIN grade 2+, the rate ratio was 1.00 (95% CI, 0.84–1.20); for CIN grade 3+, the rate ratio was 1.05 (95% CI, 0.86–1.29); and for carcinoma, the rate was 1.69 (95% CI, 0.96–2.99). Comparable results were found for the PPV ratios. Both the detection rate and PPV ratios were close to one and none of the ratios significantly differed from unity. These findings demonstrate that liquid-based cytology is neither more sensitive nor more specific than the Pap test in detecting CIN or cancer.

Comparison of Liquid-Based Cytology With Conventional Cytology for Detection of Cervical Cancer Precursors: A Randomized Controlled Trial

Comparison of Liquid-Based Cytology With Conventional Cytology for Detection of Cervical Cancer Precursors: A Randomized Controlled Trial

Incorrect Funding Source in: Comparison of Liquid-Based Cytology With Conventional Cytology for Detection of Cervical Cancer Precursors: A Randomized Controlled Trial

Incorrect Funding Source in: Comparison of Liquid-Based Cytology With Conventional Cytology for Detection of Cervical Cancer Precursors: A Randomized Controlled Trial

Comparison of Liquid-Based Cytology With Conventional Cytology for Detection of Cervical Cancer Precursors

Liquid-based cytology has been developed as an alternative for conventional cervical cytology. Despite numerous studies and systematic reviews, controversy remains about its diagnostic accuracy. To assess the performance of liquid-based cytology compared with conventional cytology in terms of detection of histologically confirmed cervical intraepithelial neoplasia (CIN). Cluster randomized controlled trial involving 89,784 women aged 30 to 60 years participating in the Dutch cervical screening program at 246 family practices. One hundred twenty-two practices were assigned to use liquid-based cytology and screened 49,222 patients and 124 practices were assigned to use the conventional Papanicolaou (Pap) test and screened 40,562 patients between April 2004 and July 1, 2006. Patients were followed up for 18 months through January 31, 2008. Screening for CIN using liquid-based cytology or conventional papanicolaou (Pap) test and the blinded review of all follow-up of screen-positive women (blinded to the type of cytology and the initial result). Intention-to-treat and per-protocol analysis of the detection rates of and positive predictive values for histologically verified CIN in both cytology systems. Outcomes are presented as crude and adjusted rate ratios (adjustment for age, urbanization, study site, and period). The adjusted detection rate ratios for CIN grade 1+ was 1.01 (95% confidence interval [CI], 0.85-1.19); for CIN grade 2+, 1.00 (95% CI, 0.84-1.20); for CIN grade 3+, 1.05 (95% CI, 0.86-1.29); and for carcinoma, 1.69 (95% CI, 0.96-2.99). The adjusted positive predictive value (PPV) ratios, considered at several cytological cutoffs and for various outcomes of CIN did not differ significantly from unity. This study indicates that liquid-based cytology does not perform better than conventional Pap tests in terms of relative sensitivity and PPV for detection of cervical cancer precursors. trialregister.nl Identifier: NTR1032.

ThinPrep are superior to conventional smears in the cytological diagnosis of subfertile men by testicular fine‐needle aspiration

The aim of this study was the comparison of liquid-based cytology (ThinPrep, TS) to conventional smears (CS) in the investigation of subfertile men with testicular fine-needle aspiration (FNA). Between January and December 2004, testicular FNA biopsies were performed in 30 subfertile men. Both TS and CS were diagnosed according to Meng classification. Features specifically recorded in each smear included sample adequacy, tissue cells preservation, contamination with red blood cells, quality of smear background, ease of cell recognition, and the cytological diagnosis. There was agreement in the cytological diagnosis between TS and CS (P = 0.88) and sample adequacy (P = 0.73). TS was superior to CS regarding cell preservation, presence of red blood cells or tissue artifacts, quality of the smear background, and cell recognition (P < 0.0001). In testicular FNA cytology, TS appear to be superior to CS in respect to cell preservation, absence of red blood cells, background quality, and cell recognition. These advantages, however, are not translated in improved cytological diagnosis.

A comparison of liquid-based cytology with conventional cytology

Objective To evaluate the 2 methods of cytologic screening to detect abnormalities of the cervical epithelium. Methods This study with 3 groups of women was performed at Selcuk University Meram Medical School between January 2004 and March 2006. In one group (paired sample for specimen collection) women were screened with conventional cytology; in another group (paired sample for specimen collection) they were screened with liquid-based cytology; and in the third group (split sample for specimen collection) they were screened by both methods. Results The rate of unsatisfactory results was lower in the liquid-based than in the conventional cytology group (6.1% vs. 2.6%; P < 0.05). More smears containing atypical squamous cells of undetermined significance were detected by the liquid-based method, but the difference was not statistically significant. Also, no statistically significant differences between liquid-based and conventional cytology were observed in the detection of other epithelial abnormalities ( P > 0.05). Conclusion The liquid-based and conventional cytology methods were found to be equivalent in the detection of cervical epithelial abnormalities.

A comparison of liquid-based cytology and Pap smear as a screening method for cervical cancer

The implementation of population-based screening for cervical cancer with Pap smear in the early sixties was set to detect and treat precancerous lesions, hopefully preventing a subsequent invasive cervical cancer. Epidemiological data indicate that organized screening has a major impact on morbidity and mortality from cervical cancer. The limited sensitivity of a single smear necessitates repeated smears in organized program. It is suggested that liquid-based cytology improves the sensitivity. The aim of this split-sample study was to compare ThinPrep liquid-based cytology with conventional Pap smear, relying on a laboratory with long-term experience of the latter. In total, 137 women with atypical Pap smear in population-based cervical screening were enrolled for the split-sample study. The performance of both techniques (ThinPrep liquid-based cytology and conventional Pap smear) were compared and validated by a histological follow-up. Women without representative histological biopsy were excluded from the study. Pap smear had sensitivity for detection of CIN2-3 of 47% compared to 66% for liquid-based material. The concordance of the two sampling techniques with the histological diagnosis was 37% and 53%, respectively, this difference being statistically significant. The proportion of reports on atypical squamous cells of undetermined significance (ASCUS) was significantly less in the liquid-based material, 4.3% compared to 8% of the conventional smears. This improved sensitivity in combination with the possibility to perform reflex testing such as HPV DNA or p16 immunocytochemistry without renewed sampling gives ThinPrep a substantial advantage and makes the liquid-based technique interesting.