Compare Seedling Establishment Research Articles

The effects of rainforest fragment area on the strength of plant-pathogen interactions.

Pathogenic interactions between fungi and plants facilitate plant species coexistence and tropical rainforest diversity. Such interactions, however, may be affected by forest fragmentation as fungi are susceptible to anthropogenic disturbance. To examine how fragmentation affects fungus-induced seed and seedling mortality, we sowed seeds of six plant species in soils collected from 21 forest fragments. We compared seedling establishment in unmanipulated soils to soils treated with fungicides. Fungicides increased germination of Toona ciliata seeds and decreased mortality of Syzygium rubicundum and Olea dioica seedlings. The fungus-induced mortality of one of these species, S. rubicundum, decreased with decreasing fragment size, indicating that its interactions with pathogenic fungi may weaken as fragments become smaller. We provide evidence that a potential diversity-maintaining plant-fungus interaction weakens in small forest fragments and suggest that such disruptions may have important long-term consequences for plant diversity. However, we emphasize the need for further research across rainforest plant communities to better understand the future of diversity in fragmented rainforest landscapes.

The Relative Risk of Invasion: Evaluation ofMiscanthus×giganteusSeed Establishment

AbstractThe sterile hybrid, giant miscanthus, has emerged as a promising cellulosic bioenergy crop because of its rapid growth rate, high biomass yields, and tolerance to poor growing conditions; these are traits that are desirable for cultivation, but also have caused concern for their contribution to invasiveness. New seed-bearing lines of giant miscanthus would decrease establishment costs for growers, yet this previously unresearched propagule source increases fears of escape from cultivation. To evaluate the consequences of seed escape, we compared seedling establishment among seven habitats: no-till agricultural field, agricultural field edge, forest understory, forest edge, riparian, pasture and roadside; these were replicated in Virginia (Blacksburg and Virginia Beach) and Georgia (Tifton), USA. We use a novel head-to-head comparison of giant miscanthus against five invasive and three noninvasive species, thus generating relative comparisons. Overall seed germination was low, with no single species achieving germination rates >37%, in all habitats and geographies. However, habitats with available bare ground and low resident plant competition, such as the agricultural field and forest understory, were more invasible by all species. Giant miscanthus seeds emerged in the roadside and forest edge habitats at all sites. Early in the growing season, we observed significantly more seedlings of giant miscanthus than the invasive and noninvasive species in the agricultural field. Interestingly, overall seedling mortality of giant miscanthus was 99.9%, with only a single 4 cm (1.58 in) tall giant miscanthus seedling surviving at the conclusion of the 6-mo study. The ability to make relative comparisons, by using multiple control species, was necessary for our conclusions in which both giant miscanthus and the noninvasive control species survival (≤1%) contrasted with that of our well-documented invasive species (≤10%). Considering the low overall emergence, increased propagule pressure may be necessary to increase the possibility of giant miscanthus escape. Knowledge gained from our results may help prepare for widespread commercialization, while helping to identify susceptible habitats to seedling establishment and aiding in the development of management protocols.

Comparing the establishment of an invasive and an endemic palm species in the Atlantic rainforest

Background: Biological invasions are one of the major causes of biodiversity loss, yet remain rather understudied in tropical environments. The Australian palm tree Archontophoenix cunninghamiana was introduced into Brazil for ornamental purposes, but has become an invasive species in urban and suburban forest patches. The substitution of A. cunninghamiana by the native palm Euterpe edulis has been proposed as a management action. Aims: We aimed to evaluate the regeneration potential of these two palm species in an Atlantic forest remnant in south-eastern Brazil where both species occur. Methods: We compared seedling establishment and seed longevity of both species through seed sowing, and also measured the contribution of A. cunninghamiana to the local seed rain and seed bank. Results: Nearly half of the non-anemochoric diaspores collected from the seed rain belonged to A. cunninghamiana, which represented a high propagule pressure in the community. The distribution of the alien palm seeds in the seed rain correlated with the distribution of nearby young and adult individuals inside the forest. Neither A. cunninghamiana nor E. edulis appeared to have a persistent seed bank in a burial experiment; seedling survival experiments suggested a much better performance for A. cunninghamiana, which had a survival rate of ca. 30% compared with a rate of only 3.5% for E. edulis. Conclusions: The results suggest a higher regeneration capacity for the alien palm over the native species when co-occurring in a forest fragment. Management actions are thus proposed to reduce a potential biological invasion process.

Seedling establishment and survival on decaying logs in boreal mixedwood stands following a mast year1This article is one of a selection of papers from the International Symposium on Dynamics and Ecological Services of Deadwood in Forest Ecosystems.

In the boreal forest, establishment of tree regeneration is tightly linked to both mast years and the availability of adequate germination beds for seedlings. We took advantage of a mast year (2006) in the eastern boreal mixedwood to compare seedling establishment in 2007 and seedling survival 2 and 4 years later on sections of fallen logs and equivalent areas of adjacent forest floor. Several factors that could explain establishment of seedlings on logs were measured, including wood resistance, density, moisture content, and C/N ratio. Our results show that small-seeded species, such as white birch ( Betula papyrifera Marsh.) and white spruce ( Picea glauca (Moench) Voss), establish preferentially on logs whereas balsam fir ( Abies balsamea (L.) Mill.), a relatively large-seeded species, establishes more often on the forest floor. Using logistic regressions, we confirmed that the probability of seedling establishment on logs declines with wood resistance, while the survival probability is inversely proportional to stand deciduous basal area. Survival rate was similar for seedlings established on the forest floor and on logs. However, none of the white birch seedlings established on the forest floor in 2007 were alive by 2011. Even following an exceptional mast year, log occurrence in eastern mixedwood stands would not suffice to obtain adequate white spruce stocking levels.

Post-fire restoration of Mediterranean forests: Testing assembly rules mediated by facilitation

Abstract In view of the importance of facilitative interactions between plants, nurse-based planting has been proposed as a restoration technique for Mediterranean vegetation. However, facilitation efficiency is known to depend on the environmental context and the particular pair of interacting species. Understanding these context- and pair-specific dependences is fundamental to understanding Mediterranean vegetation dynamics and to improving the use of nurse-based plantation for restoration. We assessed the effectiveness of nurse-based plantation and the significance for post-fire restoration of some assembly rules mediated by facilitation. In two nearby areas of different burning ages, we compared seedling establishment of 13 tall shrubs and trees planted in open ground and under nurses. Nurses and planted seedlings were selected from different life-forms. Tests of the assembly rules were provided by the partitioning of the statistical interaction effect between nurse and planted seedling life-forms in a two-factor design. Nurse-based plantation increased seedling survival 2–9 times compared to plantation in open ground, depending on the year. The higher efficiency of nurse-based plantation was consistent for the two burned areas and occurred in many species even in years with contrasting rainfall. We detected pair-specific differences in the efficiency of facilitation. This pair-specificity was partly explained by the dependence between life-forms of nurse and guest species, suggesting the existence of assembly rules. Our results confirm that nurse-based plantation would be an appropriate post-fire restoration technique in Mediterranean mountains under dry-subhumid climate, but suggest that attention to the life-form assemblage rules is needed for an efficient implementation of such technique.

Evaluation of a mechanical seed planter for transplanting Zostera marina (eelgrass) seeds

Few seagrass transplant projects worldwide have relied on seeds, and those projects using Zostera marina (eelgrass) seeds have generally found low rates of seedling establishment (<10%). We compared seedling establishment achieved by a mechanical seed planter with seeds broadcast on the sediment surface by hand. The planter injected seeds into the sediment by pumping the seeds, suspended in a gelatin-based matrix, to a benthic sled with eight planting nozzles. As a control for the gel, seeds were also injected into the sediment without gel using a hand-held pipette. Seeds were planted at a density of 300 m −2 into six replicate plots at each of three sites in the Chesapeake Bay region in September 2005, with seedling establishment measured in April 2006. Burying seeds, either with or without gel, had an overall positive effect on seedling establishment, but the effectiveness and the best method varied among sites. Mean seedling establishment for machine-planted seeds was significantly greater than for broadcast seeds at the Piankatank River site (4% vs. 1%), but not at the York (1.2% vs. 1.4%) or Spider Crab Bay (10.1% vs. 7.4%) sites. The effect of the gel was inconsistent among sites, with the highest seedling establishment (18.8%), resulting from seeds injected by pipette without gel at the Spider Crab site. Seed burial shows promise for increasing seedling establishment relative to seed broadcasting in the Chesapeake region, but further investigation of seed–sediment interactions at specific restoration sites is necessary. Low seedling establishment rates remain a bottleneck for seed-based eelgrass restoration.

Serpentine and Nonserpentine Achillea millefolium Accessions Differ in Serpentine Substrate Tolerance and Response to Organic and Inorganic Amendments

Subgrade serpentine substrates are exceptionally difficult to revegetate due to multiple limitations including low N, P, and K, low Ca:Mg molar ratios, high levels of heavy metals including Ni, Cr, and Co, low organic matter, low CEC, and low water holding capacity. To examine the influence of plant origin on the success of the revegetation of serpentine substrates, granite and serpentine accessions of Achillea millefolium were grown on subgrade serpentine substrate amended with yard waste compost, slow-release NPK fertilizer, and/or CaSO4 · 2H2O (gypsum). The goals of this study were to: (1) identify the substrate amendment combination that maximized establishment of A. millefolium on serpentine substrate, (2) compare seedling establishment, survival, and growth of the serpentine and granite A. millefolium accessions in order to determine if a serpentine edaphic ecotype of A. millefolium exists and if this ecotype is superior to the granite accession for the establishment of vegetation on serpentine substrate and (3) if a serpentine edaphic ecotype of A. millefolium does exist, what physiological features with respect to mineral nutrition convey a higher tolerance of serpentine for this ecotype than the nonserpentine ecotype. Seedling establishment, survival, and growth were greatest for A. millefolium when the subgrade serpentine substrate was amended with 30% (v/v) compost and 220 mg kg substrate−1 each of N, P, and K. The serpentine A. millefolium accession displayed a greater tolerance of the subgrade serpentine substrate, serpentine topsoil, and the amended subgrade serpentine substrate than the granite accession. Higher capacity of the serpentine A. millefolium accession for selective Ca translocation from roots to the shoot resulted in a significantly higher shoot Ca:Mg molar ratio than the granite accession and appeared to be the most important physiological feature conveying greater tolerance of the serpentine accession for serpentine substrates.

Establishing Native Grasses in a Big Sagebrush–Dominated Site: An Intermediate Restoration Step

AbstractMany semiarid rangelands in the Great Basin, U.S.A., are shifting dominance to woody species as a consequence of land degradation including intense livestock grazing and fire suppression. Whereas past rehabilitation efforts in Big sagebrush (Artemisia tridentata) steppes removed the shrub and added introduced forage grasses to successfully shift communities from shrublands to grasslands, current consensus is that native species should be included in restoration projects and that retention of some woody plants is desirable. We examined the potential for interseeding grasses into dense shrub communities as a precursor to thinning shrubs and releasing grasses from shrub interference. We compared seedling establishment of the native grass, Bluebunch wheatgrass (Pseudoroegneria spicata), with that of the Eurasia grass, Crested wheatgrass (Agropyron desertorum), in denseAr. tridentatastands. Shrubs may play an important role as nurse plants for seedling establishment (reduced solar radiation, “island of fertility” effect) but result in highly contrasting light environments and root interference for seedlings. In experimental plots, we examined effects ofAr. tridentatashade levels (0, 40, 70, and 90% reduction of solar radiation) and initial root exclusion (present/absent) on the establishment and growth ofP. spicataandAg. desertorumseedlings. With this design we evaluated the interference effects ofAr. tridentataon the two grasses and identified the most beneficial microsites for grass restoration inAr. tridentata–dominated communities. We predicted seedling survival and growth to be greater under moderate shade (40% reduction) and limited root competition than under no or strong shade conditions (0 and 90%) and unrestricted root interactions. Fifty to 85% of theP. spicataandAg. desertorumseedlings survived the dry summer months of 1995 and 1996 and the intervening winter. Neither shading nor root exclusion fromAr. tridentataaffected final seedling survival of either species. Seedling biomass of both grass species was negatively affected by initial root interactions withAr. tridentata.However, the analysis of seedling biomass variability (coefficient of variation) indicated that in all shade and root‐exclusion treatments, some seedlings of both species developed to large individuals to survive inAr. tridentata–dominated rangelands. Thus, the use of interseeding techniques shows promise for restoring herbaceous species in denseAr. tridentatastands and should be given further consideration when shrub retention is an important consideration.

Succession and Micro‐elevation Effects on Seedling Establishment of Calophyllum brasiliense Camb. (Clusiaceae) in an Amazonian River Meander Forest1

ABSTRACTI investigated the effects of successional stage and micro‐elevation on seedling establishment of Calophyllum brasiliense (Clusiaceae), a common canopy tree of seasonally flooded lowland forest along the Manú River meander zone in southeastern Peru. To compare seedling establishment between microhabitat types, I planted C. brasiliense seeds in a fully crossed experimental design of three successional stages (early, mid, and mature) and two micro‐elevations (levees and backwaters). Seedling establishment success in this study was affected by both successional stage and micro‐elevation, but micro‐elevation was most important in mid‐successional habitats. In general, seedlings in early succession experienced better conditions than in mature forest; light levels were higher, herbivory lower, and seedling growth higher. In mid‐successional forest, micro‐elevation determined habitat quality; backwaters had higher light levels, lower herbivory, and higher seedling growth and survival than levees. Mid‐successional backwaters were similar in quality to early successional forest for seedling establishment, while levees in that same successional stage were the poorest microhabitats for establishment. Although mid‐successional backwaters are similar to early succession for seedling establishment, in the long run, seedlings that establish in mid‐succession have a lower chance of reaching reproductive size before their habitat ages to mature forest than members of their cohort that established in early succession. I hypothesize that successful recruitment for C. brasiliense in the Manú River meander system requires dispersal to early successional habitat.

Effect of gap size created by thinning on seedling emergency, survival and establishment in a coastal pine forest

It is desirable and necessary to preserve the continuity of a coastal forest through reasonable management because it can provide many shelter benefits through altering the wind behavior along the shore. Thinning is an undoubtedly important measure for the continuity of forests as it provides suitable conditions for natural regeneration; however, thinning increases the risk of wind damage immediately after thinning in the coastal areas. Therefore, few thinning study related to regeneration in a coastal forest has been made. In order to test whether coastal forest of Japanese black pine ( Pinus thunbergii Parl.) requires a specific gap size created by thinning for regeneration and to compare seedling establishment among four thinning treatments, observations of emergence, survival and establishment of P. thunbergii seedlings, together with soil water content, litter, wind and light regime were made. The observations were conducted over four growing seasons in three sizes of circular gaps (the gap diameter to stand height ratios for the four gap sizes were 0.5, 1.0, 1.5 and 0.0, the control) corresponding to the four thinning treatments in Niigata shore, Japan. Results indicated that density of seedlings older than 1 year increased with gap size or canopy openness (OP). Seedling establishment was greater in 1.5 gap sizes than in any other gap sizes, while seedlings peaked near the west and north edges of the gaps but not in the gap centers exposed to direct solar radiation. Seedling growth in 1.5 gap sizes was also significantly higher than that in any others. A tendency of seedling height increasing from east to west edge and from south to north edge across the gap was observed. Only 1- or 2-year-old seedlings occurred in gap sizes of 0.0 and 0.5, therefore establishment in both gap sizes was considered as failing. The results imply that although P. thunbergii seeds can germinate in small gaps, even in under canopy, the seedlings are unable to survive. The seedlings apparently require a minimum gap size ≥1.0, or OP >30% in order to survive, and may require at least gap size ≥1.5, or OP >40% for further development into sapling. These results can be explained by the changes of microclimates, i.e. increase of light, soil water and airflow exchange, decrease of litter and canopy cover, and alleviation of the competitions for water in gaps created by thinning. Therefore, thinning strategy, especially patch-pattern thinning is potentially a viable silvicultural measure in management for the coastal pine forest. These results provide references for establishment and management of coastal P. thunbergii forests.

Succession and Micro-elevation Effects on Seedling Establishment of Calophyllum brasiliense Camb. (Clusiaceae) in an Amazonian River Meander Forest1

I investigated the effects of successional stage and micro-elevation on seedling establishment of Calophyllum brasiliense (Clusiaceae), a common canopy tree of seasonally flooded lowland forest along the Manú River meander zone in southeastern Peru. To compare seedling establishment between microhabitat types, I planted C. brasiliense seeds in a fully crossed experimental design of three successional stages (early, mid, and mature) and two micro-elevations (levees and backwaters). Seedling establishment success in this study was affected by both successional stage and micro-elevation, but micro-elevation was most important in mid-successional habitats. In general, seedlings in early succession experienced better conditions than in mature forest; light levels were higher, herbivory lower, and seedling growth higher. In mid-successional forest, micro-elevation determined habitat quality; backwaters had higher light levels, lower herbivory, and higher seedling growth and survival than levees. Mid-successional backwaters were similar in quality to early successional forest for seedling establishment, while levees in that same successional stage were the poorest microhabitats for establishment. Although mid-successional backwaters are similar to early succession for seedling establishment, in the long run, seedlings that establish in mid-succession have a lower chance of reaching reproductive size before their habitat ages to mature forest than members of their cohort that established in early succession. I hypothesize that successful recruitment for C. brasiliense in the Manú River meander system requires dispersal to early successional habitat.

Survival of Austrocedrus chilensis seedlings in relation to microsite conditions and forest thinning

Emergence, survival and growth of seedling of the conifer, Austrocedrus chilensis, were studied in plots at three sites in the Andean Patagonian forests of Argentina. The goals of this study were: (i) to improve our understanding of the relationships between microsite conditions and A. chilensis seedling survival; and (ii) to compare seedling establishment in thinned and unthinned stands of pure dense A. chilensis forest. Seedling cohorts were marked at emergence and sampled monthly during their first growing season and at the beginning of the next spring. Microsite conditions (tree canopy, understory, litter, mosses and exposed mineral soil), emergence, survival, and mortality causes (drought and predation) were recorded for each seedling. Our results show that survival and growth of A. chilensis seedlings during the first year are higher in mild and moist sites. Both, in thinned and unthinned forests, mortality is associated with drought. For pure and dense A. chilensis forest, `regeneration mode' corresponds to fine-scale gap-phase and `regeneration niche' can be characterized by moderate-to-high light environment, soil cover through low A. chilensis litter cover and high mosses, herbs and N. antarctica litter cover in summer. In winter, survival is associated with the presence of shrubs, due to a possible nurse-shrub syndrome.

The effects of fluid sowing pre-germinated seeds and transplanting on emergence, growth and yield of outdoor bush tomatoes

SUMMARYExperiments on outdoor bush tomatoes cv. Sleaford Abundance were conducted during 3 years to compare seedling establishment from fluid-sown pre-germinated seeds with that from conventionally sown dry seeds and to compare the effects on ripening and yields of these establishment methods with transplanting. Fluid-sown pre-germinated seeds emerged about 15 days earlier than conventionally sown dry seeds from sowings made in late April (mean soil temperature at seed depth from sowing to emergence ranged from 9 to 11.5 °C) and about 6 days earlier from sowings made in mid-May (mean soil temperature ranged from 12 to 15 °C). The percentage seedling emergence from pregerminated fluid-sown seeds was, on average, 57% from the earlier sowings and 65% from the later sowings, 34 and 24% higher, respectively, than from dry seeds sown conventionally. Plants from fluid-sown pre-germinated seeds were about 50% heavier than those from dry seeds at all stages of growth up to flowering. Crops established by fluidsowing started to ripen 7 days earlier than those established from dry seeds, when averaged over sowing dates and years. Yields of ripe marketable fruit &gt; 35 mm in. size from crops established from pre-germinated seeds were 35·2 t/ha, averaged over sowing and planting dates, 37% higher than from dry seeds though 51% lower than from transplants. In the two years with good growing seasons crops fluid sown in late April gave as good yields as those transplanted in late May and early June, after the last frosts, though in the cool wet year yields from fluid-sown crops were considerably lower than those from transplanted crops. Yields of ripe, marketable fruit from ‘bare-root’ plants raised from pre-germinated seeds in unheated Dutch-light structures were, on average, 48·5 t/ha, 25% higher than from plants raised in soil blocks in a heated glasshouse.