The present investigation aims to scrutinize the role of different moving walls on the hydrothermal characteristics and thermal performance of a Fe3O4-H2O ferrofluid within a wavy chamber under mixed convection. Notably, the study also takes into consideration the effects of a magnetic field and internal heat generation or absorption phenomena. To achieve this, a numerical simulation employing a compact finite difference method is conducted, focusing on key factors such as the Richardson number (0.1≤Ri≤100), Hartmann number (0≤Ha≤30), magnetic orientation (00≤γ≤900), Grashof number (Gr=104), solid concentrations (0≤ϕnp≤0.04), magnetic number (0≤δ0≤1), Prandtl number (Pr=6.8377), and internal heat generation parameter (−2≤Q≤2). All of this is undertaken while carefully managing the ferromagnetic particle volume fraction. The primary aim is to assess how different types of moving walls affect the transport behavior of the ferrofluid and to determine the impacts of the magnetic field on the hydrothermal characteristics of the ferrofluid. The outcomes of this investigation reveal that the presence of moving walls leads to a non-uniform temperature distribution within the ferrofluid, significantly affecting the overall transport behavior. The addition of ferrofluid particles to the base fluid results in noteworthy enhancements in the average Nusselt numbers, signifying improved convective heat transfer efficiency. Specifically, Case-I experienced an increase of up to 3.21%, Case-II showed an improvement of 0.93%, Case-III exhibited a rise of 1.70%, and Case-IV demonstrated the highest enhancement of 20.71%. Furthermore, the inclusion of magnetic field-dependent viscosity and the internal heat generation coefficient significantly influences the behavior of the ferrofluid. The findings from this study hold paramount importance for the design and optimization of heat transfer systems that employ ferrofluids and have the potential to contribute to the development of novel technologies with a wide range of industrial applications.