Common Human Pathogenic Bacteria Research Articles

Phyto-pharmacological Evaluation of Pimenta dioica (Linn.) Merill, Essential oil for Anthelmintic and Antimicrobial potential

Pimenta dioica (Linn.) Merill., belongs to the family Myrtaceous, has been used as a spice and is well known for its culinary as well as medicinal values. The aim of the current study was to assess the in vitro antimicrobial, anthelmintic activity and phytochemical composition of the essential oil extracted from Pimenta dioica leaves. The extraction of essential oil was performed with the Clevenger apparatus, by hydro-distillation. Pimenta dioica essential oil yield was 1.03%. The GC-MS analysis of this Pimenta dioica essential oil showed that eugenol (74.72%) was the predominant component. The essential oil showed potent anthelmintic activity assay when analysed by the adult worm’s motility assay. It has shown a comparable paralysis and disintegration profile of the model organism to that of the standard drug albendazole. The essential oil was also found to be effective against common human pathogenic bacteria, including Staphylococcus aureus, Klebsiella, Escherichia coli, Chromobacterium violaceum and Proteus vulgaris. It also showed moderate antifungal activity against Candida albicans and Aspergillus niger. These results proved that Pimenta dioica essential oil could be used as a source of the lead molecule for developing novel anthelmintic or antimicrobial agents.

Agro-ecosystem of honeybees as source for native probiotic yeasts

Probiotic microorganisms are used to improve the health and wellness of people and the research on this topic is of current relevance and interest. Fifty-five yeasts, coming from honeybee’s ecosystem and belonging to Candida, Debaryomyces, Hanseniaspora, Lachancea, Metschnikowia, Meyerozyma, Starmerella and Zygosacchromyces genera and related different species, were evaluated for the probiotic traits. The resistance to gastrointestinal conditions, auto-aggregation, cell surface hydrophobicity or biofilm formation abilities as well as antimicrobial activity against common human pathogenic bacteria were evaluated. The safety analysis of strains was also carried out to exclude any possible negative effect on the consumer’s health. The influence of proteinase treatment of living yeasts and their adhesion to Caco-2 cells were also evaluated. The greatest selection occurred in the first step of survival at the acidic pH and in the presence of bile salts, where more than 50% of the strains were unable to survive. Equally discriminating was the protease test which allowed the survival of only 27 strains belonging to the species Hanseniaspora guilliermondii, Hanseniaspora uvarum, Metschnikowia pulcherrima, Metschnikowia ziziphicola, Meyerozyma caribbica, Meyerozyma guilliermondii, Pichia kluyveri, Pichia kudriavzevii and Pichia terricola. An integrated analysis of the results obtained allowed the detection of seven yeast strains with probiotic aptitudes, all belonging to the Meyerozyma genus, of which three belonging to M. guillermondii and four belonging to M. caribbica species.

Phytochemical Analysis and Antibacterial Studies of Some Yemeni Medicinal Plants against Selected Common Human Pathogenic Bacteria

In traditional medicine, medicinal plants are often used to treat a wide range of infectious diseases in humans. Objective: The aim of this study was to identify the phytochemicals contained in thirteen parts of six Yemeni medicinal plants: Artemisia Judaica, Ficus carica L., Nerium oleander L., Olea europaea, Santolina chamaecyparissus L. and Withania somnifera and to investigate the antimicrobial activity of different concentrations of methanol extracts from the aerial part of the selected plants against S. saprophyticus, Salmonella and E. coli in vitro. Methods: Phytochemical screening was carried out using standard procedures and the antibacterial activity of different extracts of each plant was studied by agar well diffusion method. Results: Phytochemical analysis of the plant extracts revealed the presence of phenols, saponins, essential oils, flavonoids, steroids, tannins, cardiac glycosides and alkaloids. The occurrence of phytochemicals varied from plant to plant. Tannins and phenols were found in all samples, while all other test samples contained at least five of the phytochemicals tested. Almost all extracts were able to inhibit the growth of the bacterial strains, with the exception of the species S. saprophyticus, which was resistant to the methanolic extracts of A. judaica and F. carica L. However, the methanol extract (0.1 g/ml) of S. chamaecyparissus L. and O. europaea showed the largest zone of inhibition (35 mm and 20 mm, respectively) against S. saprophyticus. This is the first report on the antibacterial activity of S. chamaecyparissus L. species against S. saprophyticus. Conclusions: The present screening result shows that the methanol extract of the tested traditional Yemeni medicinal plants exhibits potent antibacterial activity, and the studied plants may represent a new source for the discovery of novel antibacterial compounds for the treatment of drug-resistant human pathogens.

Biological Studies of Harmala peganum Exracts as Antibacterial Agent

Harmala peganum is one of the most well-known medicinal plants in traditional Chinese medicine. The goal of this study was to look at the antibacterial effects of a methanol extract of several components of H. peganum, such as the stem and leaf, against certain common human pathogenic bacteria. The antibacterial capabilities of methanol extracts of the specified sections were tested using the disc diffusion method, as well as their synergism activity when combined with synthetic antibiotics. The leaf and stem extracts were the only portions of H. peganum that showed antibacterial action against all of the microorganisms tested, even at the lowest concentrations. Leaf extract out performed stem extract in terms of antibacterial activity against the majority of gram-positive bacteria tested. The extracts were tested for their biological activity against microorganisms such as Streptococcus pneumoniae, Pseudomonas aeruginosa, Salmonella typh, and Escherichia coli. Except for the distilled water extract of H. peganum, the results demonstrated that the extracts are active against all bacterium strains. However, the antibacterial activity of Harmala peganum leaf and stem extracts was higher than that of antibiotics used against the pathogens tested.

Synthesis, characterization and bioactivity of thio-acetamide modified ZnO nanoparticles embedded in zinc acetate matrix

ZnO nanoparticles embedded in zinc acetate matrix were synthesized by chemical route. The effect of thio-acetamide concentration during its synthesis was probed by structural, morphological, optical and bioactivity studies. XRD characterization indicated the formation of dominant phase of zinc acetate along with the low intensity peak of wurtzite ZnO. Morphological transition from bulky-like feature to flower-like feature via flake-like feature, is evidenced with increasing thio-acetamide molar concentrations. The optical band gap of samples decreased from ∼3.29 to 3.24 eV whereas the emitted color shifted from near green to blue region with increasing of molar concentration of thio-acetamide from 0 to 30% in the sample. The nanoparticles exhibited antimicrobial activity against seven (7) common human pathogenic bacteria including drug resistant varieties K. pneumonaie and S. aureus. The nanoparticles formed pores in the biological model membranes made from egg-phosphatidyl choline. Our study reveals that the thio-acetamide modified ZnO nanoparticles embedded in zinc acetate matrix could be used as potential drug lead to fight drug resistance against K. pneumoniae and S. aureus.

Isolation and characterization of bioactive compounds from Euphorbia cotinifolia

BackgroundGreen plants are found to be an effective reservoir for bioactive molecules and can provide appreciable sources of antimicrobial agents. Antibacterial activity of solvent extracts of Euphorbia cotinifolia leaves was tested by agar cup diffusion and broth microdilution methods against some common human pathogenic bacteria viz., Bacillus cereus, Klebsiella pneumonia, Enterobacter aerogenes, Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Salmonella typhi. The methanol extract of Euphorbia cotinifolia was subjected to a silica gel column, leading to the isolation of a bioactive compound 1. The structure of compounds was elucidated by spectroscopic techniques and assessed for their antibacterial activity against several human pathogenic bacteria.ResultsThe inhibition zone ranged against some common human pathogenic bacteria was 15.25–19.50 mm, 13.50–19.25 mm, 12–18.50 mm, 15–20 mm, and 13–19 mm for ECMF1, ECMF2, ECMF3, compounds 1, respectively. The MIC was found to be in the range 91–729 μg/ml for the fractions. The inhibition range was recorded between 12–19 and 10–14 mm for methanol and ethyl acetate extracts, respectively. K. pneumoniae, E. aerogenes, and B. subtilis were highly susceptible to methanol extract with the maximum inhibition zone of 19 mm. The MIC of the compound 1 against human pathogens was 78–833 μg/ml.ConclusionThe present study results suggest that tested plant extracts have moderate to potent antibacterial activity due to the occurrence of phenols and flavonoids in the extracts. The defensive property of natural antibacterials is mainly due to the presence of these major groups, vitamins, phenols, flavonoids, and carotenoids. In the present study, biologically active diterpene was isolated and the structures of the new diterpenoids isolated from E. cotinifolia were closely related to an ingenol ester.

Collection, Extraction, and In Vitro Antibacterial Evaluation of Plants Used in Traditional Medicine.

Medicinal plants are used to treat infectious and inflammatory diseases in various traditional medical systems, and thus could represent a promising source of antimicrobials. To establish the scientific basis for the therapeutic actions of traditional plant medicines, we provide a general workflow for evaluating the anti-infective properties of crude extracts from plants. We provide guidance starting from plant collections in the field and the creation of herbarium voucher specimens, moving to the processing of plants by drying, grinding, and extracting the plant parts collected, and finally ending with the antimicrobial investigation of these plant extracts. In this protocol, we provide a description of our workflow for the growth inhibitory evaluation of plant extracts against common human pathogenic bacteria.

Immune evaluation of a Saccharomyces cerevisiae-based oral vaccine against Helicobacter pylori in mice.

Helicobacter pylori (H.pylori) is a common human pathogenic bacterium that is associated with gastric diseases. The current leading clinical therapy is combination antibiotics, but this treatment has safety issues, especially the induction of drug resistance. Therefore, developing a safe and effective vaccine against H.pylori is one of the best alternatives. To develop Saccharomyces cerevisiae (S.cerevisiae)-based oral vaccines and then demonstrate the feasibility of this platform for preventing H.pylori infection in the absence of a mucosal adjuvant. Saccharomyces cerevisiae (S.cerevisiae)-based oral vaccines, including EBY100/pYD1-UreB and EBY100/pYD1-VacA, were generated and analyzed by Western blot, Immunofluorescence analysis, flow cytometric assay, and indirect enzyme-link immunosorbent assay (ELISA). Further, antibody responses induced by oral administration of EBY100/pYD1-UreB, EBY100/pYD1-VacA, or EBY100/pYD1-UreB+EBY100/pYD1-VacA were measured in a mouse model. Lastly, the vaccinated mice were infected with H.pylori SS1, and colonization in the stomach were evaluated. Saccharomyces cerevisiae-based H.pylori oral vaccines were successfully constructed. Mice orally administered with EBY100/pYD1-UreB, EBY100/pYD1-VacA, or EBY100/pYD1-UreB+EBY100/pYD1-VacA exhibited a significant humoral immune response as well as a mucosal immune response. Importantly, S.cerevisiae-based oral vaccines could effectively reduce bacterial loads with statistical significance after H.pylori infection. Our study shows that S.cerevisiae-based platforms can serve as an alternative approach for the future development of promising bacterial oral vaccine candidates.

A New Citrinin Derivative from the Indonesian Marine Sponge-Associated Fungus Penicillium citrinum.

Sponge-associated fungi are attractive targets for the isolation of bioactive natural products with different pharmaceutical purposes. In this investigation, 20 fungi were isolated from 10 different sponge specimens. One isolate, the fungus Penicillium citrinum strain WK-P9, showed activity against Bacillus subtilis JH642 when cultivated in malt extract medium. One new and three known citrinin derivatives were isolated from the extract of this fungus. The structures were elucidated by 1D and 2D NMR spectroscopy, as well as LC-HRMS. Their antibacterial activity against a set of common human pathogenic bacteria and fungi was tested. Compound 2 showed moderate activity against Mycobacterium smegmatis ATCC607 with a minimum inhibitory concentration (MIC) of 32 µg/mL. Compound 4 exhibited moderate growth inhibition against Bacillus subtilis JH642, B. megaterium DSM32, and M. smegmatis ATCC607 with MICs of 16, 16, and 32 µg/mL, respectively. Furthermore, weak activities of 64 µg/mL against B. subtilis DSM10 and S. aureus ATCC25923 were observed for compound 4.

Awareness, attitude, and knowledge about pneumococcal infection and vaccination: A survey across North Delhi

INTRODUCTION: Pneumococcal infection is a significant public health problem and leading cause of death among young children in developing countries. In most of the cases, “Streptococcus pneumonia” is the common human pathogenic bacterium. It further increases the risk of otitis media, meningitis, sepsis, paranasal sinuses, and upper respiratory infections. To fight against this condition advanced antibiotic therapies are available, but due to less awareness and knowledge people are unable to access.AIM: The present study aims to measure the level of awareness and knowledge among the population of North Delhi about pneumococcal infection and vaccination.MATERIALS AND METHODS: To conduct the survey, questionnaire was developed. The questionnaire consists of 17 questions, which are divided into three sections such as awareness, knowledge, and attitude. A total of 281 (males: 105; females: 176) participants participated in the present survey.RESULTS: The present study revealed that only 46.02% of females have seen advertisements related to pneumococcal, whereas 60% of males said that they have never seen any advertisement. A total of 43 (40.95%) males and 57 (32.38%) females reported that pneumococcal vaccination (PCV) may help in curing pneumococcal disease; however, only 8.57% males and 19.09% females agreed that PCV can cure the pneumococcal disease. Only 29.89% population knew that pneumococcal can also cause ear infection, whereas only 29.89% were aware of the fact that pneumococcal infection can be fatal for neonates.CONCLUSION: From the present study, we can conclude that after all the initiatives taken by government, general population still has low level of awareness about the PCV. We suggest that more campaigns, street play, advertisement, and other methods need to introduce to increase the level of awareness.

Targeted Proteomics for Studying Pathogenic Bacteria.

Mass spectrometry-based proteomics has been extensively used to map bacterial proteomes, which has led to a better understanding of the molecular mechanisms underlying bacterial infection and bacteria-host interactions. Quantitative proteomics using selected or parallel reaction monitoring is considered one of the most sensitive and specific quantitative MS-based approaches and has significantly advanced proteome studies of pathogenic bacteria. Here, recent applications of targeted proteomics for bacteria identification, biomarker discovery, and the characterization of bacterial virulence and antimicrobial resistance are reviewed among others. Results of such studies are expected to further contribute to improve the fight against the most common human pathogenic bacteria.

Antibacterial activity of Punica granatum L. peels extracts against three common human wound pathogenic bacteria and formulation of topical ointment and hydrogel pharmaceutical forms

Antibacterial activity of Punica granatum L. peels extracts against three common human wound pathogenic bacteria and formulation of topical ointment and hydrogel pharmaceutical forms

Vitamin D3 activates the autolysosomal degradation function against Helicobacter pylori through the PDIA3 receptor in gastric epithelial cells

ABSTRACTHelicobacter pylori (H. pylori) is a common human pathogenic bacterium. Once infected, it is difficult for the host to clear this organism using the innate immune system. Increased antibiotic resistance further makes it challenging for effective eradication. However, the mechanisms of immune evasion still remain obscure, and novel strategies should be developed to efficiently eliminate H. pylori infection in stomachs. Here we uncovered desirable anti-H. pylori effect of vitamin D3 both in vitro and in vivo, even against antibiotic-resistant strains. We showed that H. pylori can invade into the gastric epithelium where they became sequestered and survived in autophagosomes with impaired lysosomal acidification. Vitamin D3 treatment caused a restored lysosomal degradation function by activating the PDIA3 receptor, thereby promoting the nuclear translocation of PDIA3-STAT3 protein complex and the subsequent upregulation of MCOLN3 channels, resulting in an enhanced Ca2+ release from lysosomes and normalized lysosomal acidification. The recovered lysosomal degradation function drives H. pylori to be eliminated through the autolysosomal pathway. These findings provide a novel pathogenic mechanism on how H. pylori can survive in the gastric epithelium, and a unique pathway for vitamin D3 to reactivate the autolysosomal degradation function, which is critical for the antibacterial action of vitamin D3 both in cells and in animals, and perhaps further in humans.Abbreviations: 1,25D3: 1α, 25-dihydroxyvitamin D3; ATG5: autophagy related 5; Baf A1: bafilomycin A1; BECN1: beclin 1; CagA: cytotoxin-associated gene A; CFU: colony-forming unit; ChIP-PCR: chromatin immunoprecipitation-polymerase chain reaction; Con A: concanamycin A; CQ: chloroquine; CRISPR: clustered regularly interspaced short palindromic repeats; CTSD: cathepsin D; GPN: Gly-Phe-β-naphthylamide; H. pylori: Helicobacter pylori; LAMP1: lysosomal associated membrane protein 1; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; MCOLN1: mucolipin 1; MCOLN3: mucolipin 3; MCU: mitochondrial calcium uniporter; MOI: multiplicity of infection; NAGLU: N-acetyl-alpha-glucosaminidase; PDIA3: protein disulfide isomerase family A member 3; PMA: phorbol 12-myristate 13-acetate; PRKC: protein kinase C; SQSTM1: sequestosome 1; STAT3: signal transducer and activator of transcription 3; SS1: Sydney Strain 1; TRP: transient receptor potential; VacA: vacuolating cytotoxin; VD3: vitamin D3; VDR: vitamin D receptor

Antibacterial activity of Ag and Cu nanoparticles synthesized by chemical reduction method: a comparative analysis

In the present study, silver and copper nanoparticles were synthesized by using chemical reduction method. TGA was used to know the thermal stability and it was found that silver nanoparticles were more stable than copper nanoparticles. It was also observed from thermogram that copper nanoparticles gained small weight at 450 °C due to oxidation. DSC curves showed the peaks of endothermic and exothermic reactions that indicated the chemical changes inside nanoparticles. RD peaks demonstrated the face centered cubic crystal structure with crystallite size 15.13 nm and 14.45 nm of silver and copper nanoparticles, respectively. XRD also showed the partial oxidation of copper nanoparticles (Cu2O). Spherical morphology with average grain size ranging 120–300 nm of silver nanoparticles and 150–350 nm of copper nanoparticles was observed by SEM. It was found that silver and copper nanoparticles are significant antibacterial agent against common human pathogenic bacteria like Staphylococcus aureus and Escherichia coli. It was observed that antibacterial activity increased as concentration of samples increased. Maximum zone of inhibition was observed by silver nanoparticles at concentration 0.24 g ml−1. Copper nanoparticles results were comparable with silver nanoparticles against S. aureus but showed slightly less antibacterial action against E. coli. It was observed that silver nanoparticles were more reactive to inhibit the growth of bacteria as compared to copper nanoparticles.

Rapid Detection of Staphylococcus aureus and Related Species Isolated from Food, Environment, Cosmetics, a Medical Device, and Clinical Samples Using the VITEK MS Microbial Identification System.

Staphylococcus spp. is considered as one of the most common human-pathogenic bacteria, causing illnesses ranging from nonthreatening skin infections to lethal diseases, including sepsis, pneumonia, bloodstream infections, and food poisoning. The emergence of methicillin-resistant Staphylococcus aureus strains has increased morbidity and mortality and resulted in a major healthcare burden worldwide. Single and multilocus sequence typing have been extensively used in the identification of Staphylococcus species. Nevertheless, these assays are relatively time-consuming and require high-quality DNA. Matrix-assisted laser desorption ionization-time-of-flight has been used recently for the rapid identification of several bacterial species. In this study, we have examined 47 Staphylococcus isolates recovered from food, environment, clinical samples, cosmetic products, and a medical device and 3 American Type Culture Collection Staphylococcus reference isolates using bioMérieux VITEK MS and VITEK 2 systems to determine isolate identity. Sequencing of the 16S ribosomal RNA gene was performed to confirm and compare the species identification data generated by VITEK 2 and VITEK MS systems. Although the VITEK 2 system could not identify one of the isolates, VITEK MS identified all 50 Staphylococcus spp. isolates tested. Results of this study clearly suggest that VITEK MS can be used in the rapid identification of Staphylococcus isolates of public health importance.

Evaluation of Antimicrobial Activities of Medicinal Plant Extracts on Common Human Pathogenic Bacteria

Evaluation of Antimicrobial Activities of Medicinal Plant Extracts on Common Human Pathogenic Bacteria

Cytotoxic and Antibacterial Assessment of Stem-Barks of <i>Feretia apodanthera</i> and <i>Erythrophleum ivorense</i>; Two West African Medicinal and Socio-Economic Trees

To assess the antibacterial and cytotoxic properties of stem-barks of Feretia apodanthera and Erythrophleum ivorense extracts from powdered stem-barks of Feretia apodanthera and Erythrophleum ivorense were prepared following standard techniques of marceration, filtration and evaporation. Antibacterial activity was assayed against five pathogenic bacteria strains by the well-diffusion and broth microdilution methods. Cytotoxicity was measured by acute toxicity test on female albino rats and confirmed by cell viability assay using 3T3 cell lines. Phytochemical analysis was performed following standard techniques. The aqueous/alkaloid extracts of Feretia apodanthera and the ethanol extract of Erythrophleum ivorense were active against the five pathogenic bacteria strains tested (diameter zone of inhibition (DZI) ranging from 5.1 to 17.8mm). The Feretia apodanthera extracts were the most active against Staphylococcus aureus (DZI 17.1-17.8mm). The MIC and MBC of the extracts of both plants ranged from 0.094mg/ml to 48mg/ml and 0.047mg/ml to 48mg/ml respectively. Extracts of Feretia. apodanthera at 5000mg/Kg had no effect on the behavioural properties of rats and no death was observed. Incubation with 3T3 cell lines did not produce any cell toxicity up to 20mM and 5mM respectively for the aqueous extract and the alkaloid fraction. Incubation with higher concentrations produced cell death with IC50 of 39.41 ± 0.95mM and 38.45 ± 1.64mM respectively. Phytochemical analysis revealed the presence of various constituents. The results show for the first time that stem-bark extracts of F. apodanthera and E. ivorense possess antibacterial activities against common human pathogenic bacteria and the low/lack of toxicity as demonstrated with the F. apodanthera extracts justify and confirm their safe ethnomedical uses.

Antagonistic activity of marine bacterial isolates on common human pathogenic bacteria

Antagonistic activity of marine bacteria against common human pathogens was studied in this project. Marine animal surface bacterial flora was isolated from selected animal collected from local fishermen. The sample bacteria were isolated by serial dilution followed by spread plate method using marine agar (ZMA). About 11 bacterial isolates were selected and screened for antimicrobial activity through agar well diffusion assay. Among 11 isolates, 7 isolates exerted an inhibitory effect against human pathogenic organisms (E.coli, S.aureus, Klebsiella and Candida sp.). The isolates were then investigated for antibiotic production by cross species signal transfer mediated induction, 2 isolates (Sh-2 and PJR-3) exhibited enhanced activity against human pathogenic organisms (E.coli, S.aureus and Candida sp.). These 2 isolates were characterized phenotypically by morphological techniques (which revealed the strains as gram-positive and gram negative respectively) and physiologically by conventional tests.

Borago officinalis L. flower: a comprehensive study on bioactive compounds and its health-promoting properties

The herbal plant Borago officinalis L. flower were analysed for its bioactive compounds and antioxidant, antibacterial, anti-inflammatory and anticancer activities using different solvent polarities (methanol, ethanol and water). The RP-HPLC analyses of the methanolic extract confirmed the presence of phenolics (gallic acid, pyrogallol, salicylic acid, caffeic acid), flavonoids (myricetin, rutin) and isoflavonoid (daidzein). Besides, the major individual fatty acids of methanolic extract were α-linolenic, stearidonic, palmitic, linoleic and γ-linolenic acids. The methanolic extract possessed the highest antioxidant properties as compared to the ethanolic and water extracts. The flower methanolic, ethanolic and water extracts showed high, moderate and weak antibacterial activities against common human and foodborne pathogenic bacteria, respectively. Furthermore, the flower extracts showed weak anti-inflammatory in murine RAW 264.7 macrophage cells and low anticancer properties against human hepatic, prostate and colon cancer cells. This high-value flower could be considered as a source of putative antioxidant and antibacterial compounds to improve the human health and to be used as biopreservative in food and cosmetic industries.

Phytochemical evaluation, antioxidant properties and antibacterial activity of Iranian medicinal herb Galanthus transcaucasicus Fomin

This study was performed to determine bioactive compounds such as flavonoid and phenolic contents by HPLC and gas chromatography–mass spectrophotometry (GC–MS) and evaluate their antioxidant and antibacterial activities in bulb, flower and shoot extracts of Galanthus transcaucasicus Fomin. The overall results indicated that shoot part contained higher amounts of phenolics and flavonoids compared to the bulb and flower with respective values of 4.45and 2.67 mg/g DW. Besides, HPLC analyses showed that the gallic acid and syringic acid were the major phenolic contents in bulb, flower and shoot of G. transcaucasicus extracts. Apart from that, naringin was the main flavonoid compounds in bulb, flower and shoot. Volatile metabolites were detected by GC–MS screening that indicated 2-furancarboxaldehyde, 2,3-butanediol, and acetic acid as the main compounds in the bulb, flower and shoot extracts. The results of antioxidant activity test demonstrated that shoot extracts of G. transcaucasicus exhibited higher antioxidant activities compare to bulb and flower. Furthermore, the shoot, flower and bulb extracts showed high, moderate and weak antibacterial activities against common human and food borne pathogenic bacteria, respectively. The overall results revealed that various phytochemicals and bioactive compound in different parts of G. transcaucasicus could be considered as a source of putative antioxidant and antibacterial properties that could be used as biopreservative in food and cosmetic industries.