In penaeid shrimp, the mandibles are responsible for emitting click sounds when they collide during food intake, which has been used to assess feeding activity by passive acoustic monitoring. However, the acoustic parameters of the clicks could be affected by variations in the thickness of mandible cuticle during the molting cycle. Thus, the aim of this study was to evaluate the effects of the molting cycle on the acoustic characteristics of clicks emitted during the feeding activity of Litopenaeus vannamei fed commercial pelleted diet. Shrimp with an average weight of 8.75 ± 1.05 g were individually maintained in 24 tanks in a recirculating water system. A total of 12 animals had their feeding activity individually recorded soon after ecdysis (postmolt group), while another 12 were recorded in the middle of their molting cycle (intermolt group). Recordings took place in anechoic chambers with a hydrophone connected to a digital recorder (sampling rate of 192 kHz), and the click acoustic parameters were characterized in Raven® 1.5 Pro software. After the recordings, the shrimp had their mandibles removed for length measurement and histological analysis of the mandibular cuticle. Body (weight and length) and mandibular measurements did not differ between shrimp groups, but those in the postmolt stage showed significantly lower cuticle thickness. The acoustic characteristics of the clicks were affected by shrimp molting stages, with significantly lower maximum energy (dB) and maximum frequency (kHz) in postmolt shrimp, probably related to their lower mandibular cuticle thickness. These results may contribute to the assessment of shrimp feeding behavior associated with the molting cycle, as well as to the optimization of algorithms controlling acoustic automatic feeders in farming systems.