Thermal comfort properties are very important factors for knitted fabrics. Optimized comfort properties are demanding during the design of garments, especially for sportswear, outerwear and underwear. Knitted fabrics produced from regenerated fibers other than cotton are highly preferred due to their eco-friendly, environmentally friendly and superior performance properties. In this study, the comfort properties of woven fabrics were investigated. In this study, single jersey knitted fabrics with cotton 100%, bamboo 100%, Modal 100%, Tencel 100% and Cotton/Bamboo (50:50%), Cotton/Modal (50:50%), Cotton/Tencel (50:50%) blend ratios were used. Bursting strength, strength, pilling, air permability, thermal conductivity, thermal resistance and water-vapor permeability properties of single jersey knitted fabrics were investigated. The results of the study showed that the thermal comfort (busting strength, piiling, air permability, thermal conductivity, thermal resistance and water-vapor permeability) of Tencel 100% and Cotton/Tencel (50:50%) single jarsey knitted fabrics were the best. T-shirts were produced by evaluating the obtained data.