Combination Of BA Research Articles

Micropropagation of the endangered Aloe polyphylla

A rapid propagation protocol was establish for thehighly endangered Aloe polyphylla (Schonland ex Pillans). Seed was germinated in vitro on Murashige & Skoog 1962 medium [6] with or without sucrose. Plantlets were cultured onmedium containing benzyladenine only, or a combinationof BA and NAA. After initial problems with browning,the explants rapidly formed axillary and adventitiousbuds. Maximal shoot formation was obtained on MSmedium containing 1.0 mg l−1 BA. Some shootsrooted spontaneously on MS medium, but the rootingpercentage was improved with a 0.5 mg l−1 IBAsupplement. Rooted plantlets were acclimatized togreenhouse conditions. The success of this projectindicates that micropropagation can be a useful toolin the conservation of this endangered species of thegenus Aloe.