Combination Of Non-invasive Techniques Research Articles

Characterization of a Unique Nuragic Bronze Navicella with a Combination of X-ray Fluorescence Spectrometry and Monte Carlo Simulation

This paper describes the results obtained from an archaeometric study of a bronze Nuragic small boat model (Sardinia, Italy) dating from the Early Iron Age (presumably 9th–7th centuries BC). The artifact comes from an unknown location in Sardinia and is one of the objects that came to the Museum of Turin in the 19th century. This model is of particular interest as it is a unique Nuragic boat model containing a human figure among its decorations. The artifact is kept in the collection of the Royal Museum of Turin (Italy) and is a typical example of Sardinian manufacture from the Early Iron Age. This study was carried out using a combination of non-invasive techniques with energy dispersive X-ray fluorescence spectrometry (ED-XRF) integrated with Monte Carlo (MC) simulations and Raman spectroscopy, which allowed the characterization of the alloy of the artifact.

The Unexpected Discovery of Syngenite on Margarito d’Arezzo’s The Virgin and Child Enthroned, with Scenes of the Nativity and the Lives of the Saints (Probably 1263–4) and Its Possible Use as a Yellow Lake Substrate

The oldest painting in the National Gallery collection, The Virgin and Child Enthroned by Margarito d’Arezzo (NG564), dated to about 1263–4, depicts the Virgin and Child in a mandorla, surrounded by scenes of the Nativity and lives of the saints, set within red and decorative black borders, against a gilded background. The materials and technique were investigated using a combination of non-invasive techniques, such as Fibre Optic Reflectance Spectroscopy (FORS) and macro X-ray fluorescence scanning (MA-XRF), and the analysis of a small number of paint samples using energy dispersive X-ray analysis in the scanning electron microscope (SEM-EDS), High-Performance Liquid Chromatography (HPLC), Attenuated Total Reflectance—Fourier transform infrared spectroscopy (ATR-FTIR) and micro X-ray Diffraction (micro-XRD). The results provided evidence for the use of a number of organic colourants, with both indigo and red lake pigments identified. The finding of an unusual compound, syngenite (K2Ca(SO4)2·H2O), is here postulated as a potential substrate for an organic yellow lake pigment. In addition, reference pigments were prepared to explore this hypothesis. Although documentary evidence confirms that yellow lakes were being produced from an early date, there is very little direct evidence for their use in 13th-century panel paintings.

Forensic examination of a fragmentary funerary portrait in the collection of the Harvard art museums

The Harvard Art Museums’ collection includes six Egyptian funerary portraits of the Roman period. These portraits are all that remains of the funerary equipment of individuals whose bodies were carefully prepared for burial and the afterlife. One example, depicting a man, is particularly complicated, broken into multiple fragments which have been glued down onto a board. The in-depth study of the portrait used a combination of non-invasive techniques, including X-radiography, infrared-, ultraviolet- and visible-induced luminescence imaging, and X-ray fluorescence spectroscopy to identify and locate particular pigments, binders and other artist materials, without needing to take a sample. Targeted sampling, informed by the imaging process, was then undertaken for additional analysis through the use of cross-sections, scanning electron microscopy with energy dispersive X-ray spectrometry, Fourier transform infrared spectroscopy, Raman spectroscopy, radiocarbon dating, and lead isotope ratio analysis. This study identified a core group of three fragments in the center of the portrait that comprise much of the face and neck, tunic, and part of the hair. The remaining 15 fragments contain most of the background, parts of the hair, and the proper left eye and tunic, and are distinct from the central group of fragments. Analysis suggests these fragments were reused from other ancient funerary portraits, and whilst it was not possible to connect any of these added fragments to one another, a potential workshop connection between the central fragments and three added fragments can be suggested based on a study of the composition of the lead white pigment, and similarities in painting technique.

How Many Secret Details Could a Systematic Multi-Analytical Study Reveal About the Mysterious Fresco Trionfo della Morte?

The “Trionfo della morte” is a detached fresco painting dated at the half of the XV century. Its history is strictly connected with the history of Palermo and it is considered a symbol of the late Gothic period. Some small areas of the fresco were analyzed using a combination of non-invasive techniques and hand-held instrumentations (multispectral imaging analysis, X-ray fluorescence (XRF), and IR spectroscopy). The characterization of the nature of pigments used in its realization and restoration works was performed and some indications about its conservation state were obtained. More interestingly, some hidden details were revealed on the mysterious painting. They constitute additional evidence of the preciousness of the fresco.

Noninvasive diagnosis of hereditary transthyretin-related cardiac amyloidosis

Rationale:Cardiac transthyretin amyloidosis is a progressive and fatal cardiomyopathy for which several promising therapies are in development. The diagnosis is frequently delayed or missed because of the limited specificity of clinical manifestations, routine electrocardiogram, echocardiography and the traditional requirement for endomyocardial biopsy confirmation.Patient concerns:A 68-year-old female had suffered from lumbago for 5 years with progressive weakness, numbness in both lower limb.Diagnosis:The patient's clinical signs were not specific, but cardiac amyloidosis was suspected based on relative left ventricular apical sparing of longitudinal strain on echocardiography and continuous elevated serum levels of cardiac biomarkers (ultrasensitive cardiac troponin I and NT-proBNP). She was finally diagnosed hereditary transthyretin-related cardiac amylodosis by specific findings of cardiovascular magnetic resonance imaging (CMR), 99m-technetium pyrophosphate (99mTc-PYP) scintigraphy and genetic testing.Interventions:The patient received medications including diuretics, beta-blockers and angiotensin-converting enzyme inhibitors at the time of hospitalization. Ultimately, however, she refused further treatments and requested discharge from our hospital.Outcomes:A series of noninvasive technique enables the diagnosis of hereditary transthyretin-related cardiac amyloidosis.Lessons:While endomyocardial biopsy is not able to performed, this case demonstrates that a combination of noninvasive techniques, especially CMR, nuclear imaging, and genetic testing, may help us to make a correct diagnosis of hereditary transthyretin-related cardiac amyloidosis.

Incidence of Neoplasia in Pigs and Its Relevance to Clinical Organ Xenotransplantation.

As clinical pig organ xenotransplantation draws closer, more attention is being paid to diseases that affect pigs and those that provide a potential risk to human recipients of pig organs. Neoplasia arising from the pig organ graft is one such concern. Various tumors and other neoplastic diseases are well known to show increased incidence in organ allotransplant recipients receiving immunosuppressive therapy. Whether this effect will prove to be the case after xenotransplantation has not yet been established. Malignant tumors in young pigs are rare, with lymphosarcoma, nephroblastoma, and melanoma being the most common. The combination of noninvasive techniques and intraoperative examination of the pig organ likely will readily confirm that a pig organ graft is tumor-free before xenotransplantation. Posttransplantion lymphoproliferative disorder (PTLD) is a concern after allotransplantation, but the incidence after solid organ allotransplantation is low when compared with hematopoietic cell allotransplantation (for example, bone marrow transplantation), unless immunosuppressive therapy is particularly intensive. Organ-source pigs used for clinical xenotransplantation will be bred and housed under designated pathogen-free conditions and will be free of the γ-herpesvirus that is a key factor in the development of PTLD in pigs. Therefore if a recipient of a pig xenograft develops PTLD, it will almost certainly be of recipient origin. The increasing availability of organs from pigs genetically-engineered to protect them from the human immune response likely will diminish the need for intensive immunosuppressive therapy. Considering the low incidence of malignant disease in young pigs, donor-derived malignancy is likely to be rare in patients who receive pig organ grafts. However, if the graft remains viable for many years, the incidence of graft malignancy may increase.

Interference with Existing Memories Alters Offline Intrinsic Functional Brain Connectivity

The notion that already existing memories can be modified after their reactivation has received an increasing amount of experimental support, with empirical data accumulating across species and memory paradigms. However, there is no evidence for systems-level task-free intrinsic signatures of memory modification. Here, using a combination of behavioral, brain stimulation, and neuroimaging paradigms, we report that noninvasive transcranial magnetic stimulation interference with a reactivated motor memory altered offline task-free corticostriatal interregional functional connectivity, reducing it compared to stimulation in which the reactivated memory was intact. Furthermore, the modulated functional connectivity predicted offline memory modification. This reduction in functional connectivity recovered after additional execution of the memorized task, and the interference did not affect control cerebellar-cortical functional connectivity. This demonstrates that intrinsic task-free offline brain activity can be modulated by noninvasive interaction with existing memories and strongly correlates with behavioral measurements of changes in memory strength.

Myocarditis with fulminant type 1 diabetes mellitus diagnosed by cardiovascular magnetic resonance imaging: a case report

BackgroundFulminant type 1 diabetes is a non-autoimmune disorder characterized by sudden onset. This complication is rarely associated with myocarditis, suggesting an involvement of viral infection. We report a patient with myocarditis who was admitted for fulminant type 1 diabetes and diagnosed using a combination of non-invasive techniques.Case presentationWe describe the case of a 25-year-old Japanese man with fulminant type 1 diabetes complicated by myocarditis. The patient was admitted with flu-like symptoms and diabetic ketoacidosis, followed by chest pain the next day. Myocardial damage was suspected based on ST-segment elevation on electrocardiogram and elevation of cardiac enzymes. However, coronary angiography revealed no abnormality in the coronary arteries. We diagnosed myocarditis by a combination of echocardiography, cardiovascular magnetic resonance imaging (CMR), as well as Thallium-201 and Iodine-123 beta-methyl iodophenyl pentadecanoic acid (Tl-201 BMIPP and I-123 BMIPP) and myocardial imaging. More importantly, CMR revealed diffuse enhancement in the subepicardium of the left ventricle with late gadolinium enhancement, consistent with myocardial edema. The patient was successfully treated, received a two-week education program on diabetes and discharged without complication.ConclusionsThe rapid onset and flu-like symptoms strongly suggest the involvement of viral infection in the pathogenesis of fulminant type 1 diabetes and myocarditis. While cardiac muscle biopsy is routinely performed, this case demonstrates that a combination of non-invasive techniques, especially CMR, may successfully diagnose myocarditis in patients with fulminant type 1 diabetes.

A Novel Technical Approach to Non-Surgical Facial Rejuvenation With Intense Focused Ultrasound and Poly-L-lactic Acid Injections

Traditional rhytidectomy addresses skin laxity of the aging face without managing facial volume loss. Frequently permanent and semi-permanent filler materials are injected to replace missing volume. A common barrier to rhytidectomy acceptance for patients is the amount of post-operative recovery time. A new combination of non-invasive techniques can achieve the goals of rhytidectomy and semi-permanent fillers. We propose a technique of injectable poly-L-lactic acid to increase facial volume and the use of intense focused ultrasound light for skin tightening as non-invasive rehabilitation of the aging face with minimal recovery time.

Echophonocardiographic Diagnosis of Left Ventricular Pseudoaneurysm

We report the presence of an unusual systolic murmur associated with a traumatic left ventricular pseudoaneurysm. Echophonocardiographic studies showed the murmur to begin at the first heart sound, but end well before the second heart sound. It seems likely that the murmur is caused by the systolic flow of blood from the left ventricle into the relatively noncompliant pseudoaneurysm. The echocardiographic scan of the left ventricle demonstrated a relatively echo-free space posterior to the left ventricular wall, supporting the diagnosis of pseudoaneurysm, which has confirmed with angiographic studies and at surgery. These findings indicate that a combination of noninvasive techniques is useful in establishing this diagnosis.