Color Markers Research Articles

The seventh species of the newt genus Tylototriton in Thailand: a new species (Urodela, Salamandridae) from Tak Province, northwestern Thailand.

A new species of the crocodile newt genus Tylototriton from Doi Soi Malai located at Mae Tuen Wildlife Sanctuary, Tak Province, northwestern Thailand is described based on molecular and morphological evidence, and named as Tylototritonsoimalai sp. nov. The new species is the seventh recorded species of the genus Tylototriton reported in Thailand. It differs morphologically from its congeners by a combination of the following morphological characteristics: head longer than wide; snout blunt or truncate; sagittal ridge on head narrow, short and distinct; dorsolateral bony ridges on head pronounced and rough; parotoids distinct; vertebral ridge prominent, wide and not segmented; 14-16 distinct, rounded and isolated rib nodules but posterior nodules connected; tips of fore- and hind limbs overlapping when adpressed along the body. The body background color is black, while the color markings are orange. Molecular analysis indicated that Tylototritonsoimalai sp. nov. is a distinct lineage and sister to T.uyenoi with a 4.1% genetic sequence divergence based on the mitochondrial NADH dehydrogenase subunit 2 gene. The new species is currently restricted to the hill evergreen forests of Doi Soi Malai. The implementation of a strategic plan is recommended to protect both the species and its habitat from anthropogenic activities.

A SEMIOTIC STUDY OF CARICATURE: THE STRUGGLE BETWEEN AMERICA AND CHINA

Visual communication is a compound process, which requires broad knowledge of semiotics. Semiotics is the education of signs and symbols. In what way these signs and symbols are interpreted is studied under semiotics. It has many concealed signs and connotations, for example: brand name, logo, package design, color, punch line and trade mark, etc. The present study presents one of the most significant philosophies and replicas of visual social semiotics, namely, Kress and van Leeuwen’s "Grammar of Visual Design". It involves the description of semiotic resources, what can be said and done with images (and other visual means of communication) and how things people say and do with images can be interpreted. Thus, the communicative values of signs in a caricature are a portrait with the volume turned up. A caricature is a painting, or more commonly a picture, of an object or item in which the characteristics and structure have been blurred and exaggerated to ridicule or satirize the topic. Thus the current study aims at studying some caricatures nonverbally through analyzing the image and colours of it. The study tackles semiotics; specifically social semiotics, caricature, the elements of analyzing caricature, which are: image, word, and colour. The first part carries on the introduction of the current study as well as tackling the problem of the study, the literature review of semiotics in general and social semiotics in particular. The second part carries on the data and its analysis through the model, as well as the visual results, finally, giving the conclusion.

Enhancing leaf disease detection accuracy through synergistic integration of deep transfer learning and multimodal techniques

The agricultural sector, a cornerstone of economies worldwide, faces significant challenges due to plant diseases, which severely affect crop yield and quality. Early and accurate detection of these diseases is crucial for effective mitigation strategies. The current methods used often lack accuracy and adaptability, especially in diverse environmental conditions. This study introduces a novel, synergistic approach that integrates deep transfer learning with multimodal techniques, specifically canny edges, colour spectrum intensity analysis, and custom data augmentation strategies. Unlike existing methods that rely solely on pre-trained models, the approach utilised in this study offers an innovative fusion of distinct feature extraction techniques. The canny edges highlighted the structural intricacies of leaf diseases, while colour spectrum intensity analysis enhanced the detection of disease-specific colour markers. The customized data augmentation techniques employed (in the study) was shown to enhance the learning process of the models, resulting in their adaptability to diverse agricultural environments. This integration applied to DenseNet201 and EfficientNetB3, achieved detection accuracies of 99.03 % and 98.23 %, respectively, surpassing traditional models and setting new benchmarks in plant disease detection. These results demonstrate the effectiveness of the proposed multi-faceted approach and its potential to significantly enhance crop disease management systems.

Haptic experience to significantly motivate anatomy learning in medical students

BackgroundCurrently, multiple tools exist to teach and learn anatomy, but finding an adequate activity is challenging. However, it can be achieved through haptic experiences, where motivation is the means of a significant learning process. This study aimed to evaluate a haptic experience to determine if a tactile and painting with color marker interactive experience, established a better learning process in comparison to the traditional 2D workshop on printed paper with photographs.MethodsPlaster bone models of the scapulae, humerus and clavicle were elaborated from a computerized scan tomography. Second year undergraduate medical students were invited to participate, where subjects were randomly assigned to the traditional 2D method or the 3D plaster bone model. A third group decided not to join any workshop. Following, all three groups were evaluated on bone landmarks and view, laterality, muscle insertions and functions. 2D and 3D workshop students were asked their opinion in a focus group and answered a survey regarding the overall perception and learning experience. Evaluation grades are presented as mean ± standard deviation, and answers from the survey are presented as percentages.ResultsThe survey demonstrated the students in the 3D model graded the experience as outstanding, and in five out of the six questions, answers were very good or excellent. In contrast, for students participating in the 2D workshop the most common answers were fair or good. The exception was the answer regarding the quiz, where both groups considered it good, despite the average among all groups not being a passing grade.ConclusionsTo learn the anatomy of the shoulder, the conventional methodology was compared with a haptic experience, where plaster bone models were used, enabling students to touch and paint on them. Based on the focus group and survey this study revealed the 3D workshop was an interactive experience where, the sense of touch and painting greatly contributed to their learning process. Even though this activity was useful in terms of learning bone landmarks, view muscle insertions, and establish relations, further activities must be developed to increase their understanding regarding their function, and its relevance in a clinical setting.

Automatic detection of color markings and numbers on trees in point clouds from Personal Laser Scanning (PLS) and Terrestrial Laser Scanning (TLS)

In forestry, colored spray paint is used for different purposes, such as marking trees that are chosen for the next harvest or that will be promoted by future silvicultural activities, or for the demarcation of skid/cable roads. Numerous studies have demonstrated the application of Terrestrial Laser Scanners (TLS) and Personal Laser Scanners (PLS) for the acquisition of tree parameters (diameters, heights, and tree positions) in a forest inventory context. However, no studies have included classification based on spectral information by applying colored markings to trees. When a laser scanner is combined with a camera, the color information can be mapped onto the laser point cloud, allowing for additional analysis of spectral information. In this study, we have tested the analysis of joint information from imagery and 3D point clouds in two different settings: (i) PLS point cloud data from a GeoSLAM ZEB Horizon were complemented with spectral information from images collected with a NCTech iSTAR Pulsar 360-degree camera, (ii) TLS point cloud data from a RIEGL VZ-400i were combined with imagery from a Nikon D850 digital single lens reflex (DSLR) camera with 14 mm fisheye lens. The major objective was to automatically classify trees that were marked with colored sprays automatically identify the numbers sprayed on the trees. The marked trees could be classified correctly with a balanced accuracy of 77.26% for PLS and 98.01% for the data collected with the TLS system using a support vector machine (SVM) model. The numbers written on the trees were classified from the TLS data using a YOLOv8 model. The accuracy of the digit classification reached up to 97.30%. In conclusion, it is possible to automatically detect marked trees and to automatically recognize the numbers spayed on these trees from colored point cloud data. However, accuracy strongly depends on the selected combination of scanner and camera. The findings of future research on this subject might differ with the ongoing development of PLS systems and could be enhanced by creating a large dataset of handwritten numbers collected using laser scanning systems.

Contact point marking with the OccluSense system-an invitro study on reliability and validity.

Occlusal contacts can be stained and thereby visualised employing occlusal indicators. Qualitative and quantitative indicators are differentiated. The hybrid system OccluSense supposedly combines analogue contact marking and digital registration of contacts. The reliability and validity of its contact marking have not yet been validated. The aim of this controlled randomised in-vitro study was to examine the reliability and validity of analogue contact staining with OccluSense compared to Arti-Fol and the reference standard Gnatho-Film. An experimental apparatus was designed to simulate static occlusion. The occlusal contacts were stained 50 times with Gnatho-Film to determine the average number and location of contacts registered. For comparison, the contacts were pre-stained with Gnatho-Film and then registered with Arti-Fol or OccluSense 50 times each. Every staining was statistically evaluated and compared to Gnatho-Film alone, regarding the number and location of contacts. Based on the observed characteristic staining behaviour of OccluSense, a modified evaluation strategy was developed for its colour markings (OccluSense (mod.)). Both evaluations of OccluSense determine that the same number of contacts was registered in over 94% of all cases. Neither OccluSense nor Arti-Fol stain the exact same number of contacts as Gnatho-Film, but the modified evaluation OccluSense (mod.) did improve the results for validity. When employing the modified evaluation strategy, OccluSense staining achieves similar reliability and validity as the reference standard. Pre-colouring of occlusal contacts with Gnatho-Film and the modified evaluation strategy, might become standard for analysing occlusal contacts stained by OccluSense in the future.

Laser-induced color reversal on metal surfaces in acetic acid solution

Laser coloring for decoration and visual attraction has been widely used in daily life. To fulfill the requirements of timely correction or modification of colors, color erasure has also become an important part in marking technology. The thickness of the oxide layer and the concentration of oxygen elements are key factors affecting the quality of color erasing. The conventional method is to erase the color in air, which is difficult to remove the oxide film. Acetic acid, as an environment-friendly solution, which provides a low oxygen environment and can undergo chemical reactions with titanium dioxide under laser heating, is selected as a liquid environment for laser-induced color erasure. Oxygen concentration in the erased area decreases from more than 50% to 10%. The color difference ΔEab* between the erased area and original metal surface is around 1.4, which shows almost no difference to the naked eyes. Furthermore, the color difference ΔEab* between laser re-coloring and laser coloring before the erasure is around 1.5, which means that the re-coloring under the same laser processing parameters after the erasure maintains a high level of consistency with the colors initially drawn. Here we successfully realize the reversal process from laser coloring to laser erasure and laser re-coloring on metal surfaces. This technology has considerable applications in the field of anti-counterfeiting and information storage. Meanwhile, acetic acid assisted laser color erasing as a safe and green method greatly improves the erasure quality and enhances the development of laser color marking technology.

An Early Investigation into Raised-Line Tactile Graphics Reading Behavior among Blind and Visually Impaired Individuals

It has always been a challenge for blind and visually impaired (BVI) people to comprehend the information on tactile graphics materials. Despite the advancements in tactile graphic design, a comprehensive understanding of how they explore and interpret raised-line tactile graphics is still lacking. To bridge this gap, this paper introduces a fingertip tracking system employing color marker detection algorithm to investigate the cognitive process of four totally blind participants associated with the Malaysian Association for The Blind (MAB). Each participant was provided with five distinct kinds of raised-line tactile graphics for reading, and their reading activities were afterwards recorded. All participants exhibited diverse interpretations, exploration times, and difficulty perceptions, showcasing the subjective nature of cognitive processing. It is identified that the complex diagram took longer time of exploration with an average time of 141 seconds compared the simple diagram with an average time of 27.5 seconds. It shows that the complexity of the diagrams did impact participants' cognitive process. Based on the distribution and concentration of fingertip locations, every tactile graphic has salient regions that have been focused on by every participant for recognition and interpretation of the information. It was also identified that the frequently performed exploratory procedures among participants were contour following and lateral motions. However, their reading strategies were too random and lacked systematic patterns. The system aims to be an instrument for visualising the cognitive process underpinning the exploration and interpretation of tactile graphics by people with visual impairment and blindness.

Subpleural pulmonary nodule marking with patent blue V dye prior to surgical resection.

Subpleural located pulmonary nodules are perioperatively invisible to the surgeon. Their precise identification is conventionally possible by palpation, but often at the cost of performing a thoracotomy. The aim of the study was to evaluate the success rate and feasibility of the pre-operative CT-guided marking subpleural localized nodule using a mixture of Patent Blue V and an iodine contrast agent prior to the extra-anatomical video-assisted thoracoscopic surgery (VATS) resection in patients for whom the primary anatomical resection in terms of segmentectomy or lobectomy was not indicated. The data of consecutive patients with pulmonary nodules located ≤ 30 mm from the parietal pleura, who were indicated for VATS extra-anatomical resection between 2017 to 2023, were retrospectively reviewed and analyzed. All patients indicated for VATS resection underwent color marking of the area with the pulmonary lesion under CT-guided control immediately before the surgery. The primary outcome was the marking success. Morphological lesion characteristics, time from marking to the surgery, procedure related complications, final histology findings and 30day mortality were analyzed. Additionally, we assessed the association of the successful marking and the patient's smoking history. A total of 62 lesions were marked. The successful marking was observed in 56/62 (90.3%) patients. The median time from the lesion marking to the beginning of surgery was 75.0 (IQR 65.0-85.0) minutes. The procedure related pneumothorax was observed in 6 (9.7%) patients, intraparenchymal hematoma in 1 (1.6%) patient. No statistically significant association of the depth of the subpleural lesion's location, occurrence of complications or time from the marking to surgery and the successful marking was observed. The 30day mortality was zero. No association of smoking and successful marking was observed. The method of marking the subpleural pulmonary lesions under CT-guided control with a mixture of Patent Blue V and iodine contrast agent is a safe and effective method with minimal complications. It provides surgeons the precise visualization of the affected pulmonary parenchyma before the planned extra-anatomical VATS resection.

Identification of a candidate gene for the I locus determining the dominant white bulb color in onion (Allium cepa L.).

Through a map-based cloning approach, a gene coding for an R2R3-MYB transcription factor was identified as a causal gene for the I locus controlling the dominant white bulb color in onion. White bulb colors in onion (Allium cepa L.) are determined by either the C or I loci. The causal gene for the C locus was previously isolated, but the gene responsible for the I locus has not been identified yet. To identify candidate genes for the I locus, an approximately 7-Mb genomic DNA region harboring the I locus was obtained from onion and bunching onion (A. fistulosum) whole genome sequences using two tightly linked molecular markers. Within this interval, the AcMYB1 gene, known as a positive regulator of anthocyanin production, was identified. No polymorphic sequences were found between white and red AcMYB1 alleles in the 4,860-bp full-length genomic DNA sequences. However, a 4,838-bp LTR-retrotransposon was identified in the white allele, in the 79-bp upstream coding region from the stop codon. The insertion of this LTR-retrotransposon created a premature stop codon, resulting in the replacement of 26 amino acids with seven different residues. A molecular marker was developed based on the insertion of this LTR-retrotransposon to genotype the I locus. A perfect linkage between bulb color phenotypes and marker genotypes was observed among 5,303 individuals of segregating populations. The transcription of AcMYB1 appeared to be normal in both red and white onions, but the transcription of CHS-A, which encodes chalcone synthase and is involved in the first step of the anthocyanin biosynthesis pathway, was inactivated in the white onions. Taken together, an aberrant AcMYB1 protein produced from the mutant allele might be responsible for the dominant white bulb color in onions.

Gene Editing in the Chagas Disease Vector Rhodnius prolixus by Cas9-Mediated ReMOT Control.

Rhodnius prolixus is currently the model vector of choice for studying Chagas disease transmission, a debilitating disease caused by Trypanosoma cruzi parasites. However, transgenesis and gene editing protocols to advance the field are still lacking. Here, we tested protocols for the maternal delivery of CRISPR-Cas9 (clustered regularly spaced palindromic repeats/Cas-9 associated) elements to developing R. prolixus oocytes and strategies for the identification of insertions and deletions (indels) in target loci of resulting gene-edited generation zero (G0) nymphs. We demonstrate successful gene editing of the eye color markers Rp-scarlet and Rp-white, and the cuticle color marker Rp-yellow, with highest effectiveness obtained using Receptor-Mediated Ovary Transduction of Cargo (ReMOT Control) with the ovary-targeting BtKV ligand. These results provide proof of concepts for generating somatic mutations in R. prolixus and potentially for generating germ line-edited lines in triatomines, laying the foundation for gene editing protocols that could lead to the development of novel control strategies for vectors of Chagas disease.

Efektivitas Penggunaan Media Pembelajaran Interaktif dalam Pembelajaran Matematika di Sekolah Dasar

Efektivitas Penggunaan Media Pembelajaran Interaktif dalam Pembelajaran Matematika di Sekolah Dasar

Cyto-swapping in maize by haploid induction with a cenh3 mutant.

Maize mutants of the centromeric histone H3 (CENP-A/CENH3) gene can form haploids that inherit only chromosomes of the pollinating parent but the cytoplasm from the female parent. We developed CENH3 haploid inducers carrying a dominant anthocyanin colour marker for efficient haploid identification and harbouring cytoplasmic male sterile cytoplasm, a type of cytoplasm that results in male sterility useful for efficient hybrid seed production. The resulting cytoplasmic male sterility cyto-swapping method provides a faster and cheaper way to convert commercial lines to cytoplasmic male sterile compared to conventional trait introgression.

Effect of sucrose content added to milk on the composition, yield, viscosity, color, and Maillard reaction chemical markers of dulce de leche

Effect of sucrose content added to milk on the composition, yield, viscosity, color, and Maillard reaction chemical markers of dulce de leche

Single Colour Marks a Gray Area- An Attempt to Re-tool the Indian Legal Framework

The idea of trademarks is not a recent invention; it dates back over a considerable period of time. However, as times changed, it significantly evolved and got refined. With new developments and innovations have come about over time, different classes of marks have requested protection in the area of trademark law. However, not all of these marks were accepted to the same degree. One such particular class of mark is the single colour mark. The tussle over the registrability of single colour marks is the ongoing discourse which has the potential to generate wide-ranging ramifications for the trademark law. Different jurisdictions have attempted to solidify their legislative framework in response to single colour marks. However, there exist jurisdictions like India which are still searching for the correct path. The present paper makes an attempt to contribute to the existing set of literature by structuring the approach in relation to the issue of registering and validating the single colour marks within Indian legal framework. The paper makes an exploration to the Acts, Rules. Judicial Dictums and suitable foreign learnings in a structured manner for unravelling the conundrum persisting over the validity of single colour marks within the jurisdiction of India.

Do redundant graphical attributes reduce decision-making efficiency?

Multiple time-series graphs are commonly used for data visualisation, but few scholars have investigated the impact of graphical attributes on decision-making efficiency. This study explores the effects of graphical attributes of varying redundancy conditions on decision-making efficiency. Two experimental conditions were developed for the experiment: non-redundant (independent graphical attributes: colour, linear and marker) and redundant (combinations of two and more graphical attributes: colour and linear, colour and marker, etc.). A total of 60 people took part in both experiments and performed two tasks: maximisation and discrimination. The experiments revealed that the addition of attributes, such as colour, marker or linear, decreased response time (RT), but the combination of colour & linear & marker increased RT. This is more significant in discrimination tasks. We provide empirical evidence for the design of time-series data visualisations and encourage the combination of two of these graphical attributes, such as colour & linear, colour & marker or linear & marker, when conditions allow, to improve decision-making efficiency.

Identification of the chaA and fwA Spore Color Genes of Aspergillus nidulans.

Wild-type Aspergillus nidulans asexual spores (conidia) are green due to a pigment that protects the spores against ultraviolet light. The pigment is produced by a biosynthetic pathway, the genes of which are dispersed in the genome. The backbone molecule of the pigment is a polyketide synthesized by a polyketide synthase encoded by the wA gene. If wA is not functional, the conidia are white. The polyketide is modified by a laccase encoded by the yA gene and inactivation of yA in an otherwise wild-type background results in yellow spores. Additional spore color mutations have been isolated and mapped to a locus genetically, but the genes that correspond to these loci have not been determined. Spore color markers have been useful historically, and they remain valuable in the molecular genetics era. One can determine if a transforming fragment has been successfully integrated at the wA or yA locus by simply looking at the color of transformant conidia. The genes of the potentially useful color loci chaA (chartreuse conidia) and fwA (fawn conidia) have not been identified previously. We chose a set of candidate genes for each locus by comparing the assembled genome with the genetic map. By systematically deleting these candidate genes, we identified a cytochrome P450 gene (AN10028) corresponding to chaA. Deletions of this gene result in chartreuse conidia and chartreuse mutations can be complemented in trans by a functional copy of this gene. With fwA, we found that the existing fawn mutation, fwA1, is a deletion of 2241 base pairs that inactivates three genes. By deleting each of these genes, we determined that fwA is AN1088, an EthD domain protein. Deletion of AN1088 results in fawn conidia as expected. Neither deletion of chaA nor fwA restricts growth and both should be valuable target loci for transformations. Combinations of deletions have allowed us to investigate the epistasis relationships of wA, yA, chaA and fwA.

Integrating computer vision algorithms and RFID system for identification and tracking of group-housed animals: an example with pigs.

Precision livestock farming aims to individually and automatically monitor animal activity to ensure their health, well-being, and productivity. Computer vision has emerged as a promising tool for this purpose. However, accurately tracking individuals using imaging remains challenging, especially in group housing where animals may have similar appearances. Close interaction or crowding among animals can lead to the loss or swapping of animal IDs, compromising tracking accuracy. To address this challenge, we implemented a framework combining a tracking-by-detection method with a radio frequency identification (RFID) system. We tested this approach using twelve pigs in a single pen as an illustrative example. Three of the pigs had distinctive natural coat markings, enabling their visual identification within the group. The remaining pigs either shared similar coat color patterns or were entirely white, making them visually indistinguishable from each other. We employed the latest version of the You Only Look Once (YOLOv8) and BoT-SORT algorithms for detection and tracking, respectively. YOLOv8 was fine-tuned with a dataset of 3,600 images to detect and classify different pig classes, achieving a mean average precision of all the classes of 99%. The fine-tuned YOLOv8 model and the tracker BoT-SORT were then applied to a 166.7-min video comprising 100,018 frames. Results showed that pigs with distinguishable coat color markings could be tracked 91% of the time on average. For pigs with similar coat color, the RFID system was used to identify individual animals when they entered the feeding station, and this RFID identification was linked to the image trajectory of each pig, both backward and forward. The two pigs with similar markings could be tracked for an average of 48.6min, while the seven white pigs could be tracked for an average of 59.1min. In all cases, the tracking time assigned to each pig matched the ground truth 90% of the time or more. Thus, our proposed framework enabled reliable tracking of group-housed pigs for extended periods, offering a promising alternative to the independent use of image or RFID approaches alone. This approach represents a significant step forward in combining multiple devices for animal identification, tracking, and traceability, particularly when homogeneous animals are kept in groups.

Маркер кара в тюркской этнонимике XII в.

Research Objectives: This article is devoted to the study of some aspects of the problem of the origin of ethnonyms with color markers among the nomadic Turkic-speaking peoples. They are analysed only in regard to the two-component ethnonyms where the word denoting the name of the color is used as an attributive syntactically. In the academic literature, there are several views on the practice of using such markers and various explanations of their registry and semantics. The authors of the article proceed from the consideration that any explanations being imposed universally are premature and no generalizations can help to explain all the specific examples attested in the historical and ethnographic literature. They should be viewed for each chronological period and specific region since the practice of using color markers had its own characteristics, being, apparently, due to various factors. Thus, the marker should be identified directly with narrow specific source data. Written sources, however, contain almost no direct data to help in the problem. Research Materials: The authors of the article used a Persian-language source of the 12th century, “ ʻEkd al-ʻOlā leʼl-mawkef al-aʻlā” by Afẓal al-Dīn Kermānī. Results and Novelty of the Research: Information presented in the source mentioned a rare example to allow light to be shed on a specific case. There is a passage about a group of Ghūzz had to arrive in Kermān from Khurāsān. They also are named in the text as Qarā Ghūzz. The fact allows to confirm that, at least in the second half of the 12th century among the tribes of the Oghuzs, the color marker qara was used to denote a secondary community that broke away from the initial collective, the name of which is represented by the second element of a two-component ethnonym with a color marker.

Interactive experience of sculpture design based on virtual reality technology

Abstract The application of computer technology provides new ideas for the design of sculpture works. In this paper, with the support of virtual reality technology, the pixel set of each color block is extracted from the actual image to determine the location of the center point of the color block. Based on the extracted information, a visual color marking method is used to realize the 3D registration of the sculpture scene. In the virtual scene, through the virtual modeling of the sculpture work, a spline surface model was generated by applying sparse image sequences and optimally adjusted to transform the surface piece control point problem into a quadratic function of the network vertices. The mathematical logic properties of the virtual reality sculpture work wind direction improved by 22.5 points, the mathematical logic properties of the light design improved by 25.8 points, and the mathematical logic properties of the shadow design improved by 23.1 points. The virtual reality technology provides an interactive experience for the design of sculpture works.