Color Of Microplastics Research Articles

Methods to characterize microplastics: case study on freshwater fishes from a tropical lagoon in Colombia.

Aquatic ecosystems face multiple anthropogenic pressures, and recently plastic pollution is one of the main problems. The Luruaco lagoon receives discharges from streams with high levels of microplastics (MPs) from urban areas that impact the resident ichthyofauna. We studied the prevalence, abundance and physical and chemical characteristics of MPs in fishes in the Luruaco Lagoon, Colombia. Four samplings were carried out where fish were captured with trawl nets. Each individual was assessed for total weight, total and standard length, and then a uroventral cut was made to extract stomach and intestine (GIT). Each structure was weighed, measured, and deposited in glass jars with filtered KOH solution. The abundance of MPs and frequency of occurrence were assessed. A principal component analysis (PCA) was used to describe the variation of the species dataset (%FO, proportion of MPs and their diet). Seven species were recorded and 271 individuals examined: Microplastics were identified in all species, and 1157 particles were found in their GIT, with a prevalence of 87.5% of MPs. Mugil liza and Andinoacara latifrons had a high proportion of MPs. The highest %FO was found in cichlid species. Four typologies and 13 colors of MPs were analyzed; fibers and color blue were predominant. A direct relationship was observed between the type of diet and the proportion and %FO of MPs.

Assessment of the water quality pollution index and ecological risk of microplastic pollution along the Tambakoso River in Surabaya, Indonesia

Increasing human activities and improper waste disposal will cause microplastic pollution in surface water. This study analyzed the abundance and characteristics of microplastics, pollution index based on water quality and its relationship with microplastic pollution, and the potential ecological risk of microplastics along the Tambakoso River which is influenced by various land uses of housing, industry, agriculture, and ponds from 16 sampling points. The average abundance of microplastics in the river was 91.80 particles/L. The Kruskal Wallis test showed that there were significant differences between microplastic pollution at each sampling location (Pvalue <0.05). In general, microplastics were mostly found in the form of fragments (48.36 %), transparent color (73.81 %), SMP size (<1 mm) (81.6 %), and dominated by PVC and nylon polymers. However, the characteristics of microplastics at each sampling location varied. The water quality pollution index value showed a slightly polluted category at most points. Redundancy analysis (RDA) showed that the characteristics of the shape and color of microplastics correlated with water quality parameters. The potential ecological risk based on microplastic pollution showed minor, moderate, and high categories at points with industrial land use. This indicates that the distribution of microplastics is closely related to human activities in the area. The level of ecological risk from microplastics depends on the percentage of each plastic polymer, along with its abundance in the environment. This study offers an important basis for designing efficient countermeasures to reduce microplastic pollution and improve water quality in surface waters.

Microplastics, as a risk factor in the development of interstitial lung disease- a preliminary study

Microplastic (MPs) pollution is a global concern that affects all living organisms, yet research on MP-related disorders in humans, including incidence and symptoms, remains limited. In this study, the presence, composition, and characterization of MPs in bronchoalveolar lavage (BAL), which reflects lung tissue, and blood were examined. Fiberoptic bronchoscopy was performed to collect BAL samples from patients suspected of having interstitial lung disease (ILD) as well as from a control group. MPs were identified and measured using μ-Raman techniques.In BAL samples, the most common MPs color observed was grey/white, with sizes ranging from 4.19 to 792.00 μm. The particle shapes and polymer types identified included polyamide (PA), polyester (PET), polyvinyl chloride (PVC), and polyurethanes (PU). For blood samples, MPs were predominantly grey/white and blue, with sizes ranging from 13.14 to 20. 29 μm. The identified polymers in blood samples included polyamide (PA) and polyethylene (PE). MPs were detected in 10 out of 18 patients (55%) suspected of having ILD, with most of these patients presenting with the fibrotic type of the disease. In the control group, two patients whose BAL samples were positive for MPs were found to have chronic lung disease.This study is the first to explore the relationship between interstitial lung disease (ILD) and microplastics (MPs), revealing a tendency for the presence of MPs in the bronchoalveolar lavage (BAL) of ILD patients, particularly those with a fibrotic phenotype. Further research is needed to determine the cumulative effects of MPs on human health, especially concerning the respiratory system, which is highly exposed to environmental pollutants.

Monitoring of contamination by microplastics on sandy beaches at Vulcano Island (Sicily, Italy) by hyperspectral imaging.

In this work, the monitoring and characterization of large microplastics (1-5mm) collected from sandy beaches of Vulcano Island (Aeolian Islands, Sicily, Italy) were carried out for the first time. Microplastics were sampled from two beaches, "Gelso" and "Sabbie Nere," in three different time periods. The following characteristics of microplastic samples were assessed: quantity, distribution, categories, color, polymer type, size, and shape parameters. The polymers were identified using hyperspectral imaging, whereas an automatic image analysis approach was employed to determine microplastics' morphological and morphometrical attributes. Finally, the microplastic diversity integrated index was computed to obtain information on the potential emission sources of microplastics. It was found that the concentration of microplastics varies from 0.27 particles/kg_dw to 1.35 particles/kg_dw with fragment being the main collected category, with minor amount of pellet, foam, film, and filament. The predominant color of microplastics was by far white, followed by blue and yellow. The identified polymers were polyethylene and polypropylene followed by expanded polystyrene, polyamide, polystyrene, and polyethylene terephthalate. The morphological and morphometrical characterization highlighted a large variability for most size and shape parameters. Finally, the Microplastics Diversity Integrated Index results showed average indices compared to the literature, with higher values for the "Gelso" site (0.656), indicating a higher heterogeneity of sources, with respect to "Sabbie Nere" beach (0.530).

Abundance of microplastics in Cisadane river - Indonesia

Abstract Microplastics have become a serious threat to the aquatic environment, water treatment facilities, and riverside residents because they are persistent. Microplastics generally come from plastic waste produced by human and industrial activities that enter the rivers. In this study, the Cisadane River was studied for microplastic presence. Sampling was conducted at 11 points along the Cisadane River from the upstream (Muria Jaya) to the downstream (Teluk Naga). Samples were taken using a 200-mesh plankton net and then analyzed using a digital microscope for the microplastic’s presence, sizes, and shapes. Furthermore, material identification was conducted using a Raman Spectroscopy Microscope to determine the material type of microplastic in the samples. According to the result, the Cisadane River contains microplastics of various shapes and sizes. The result shows the abundance of microplastics of 0.8-9.6 particles/m3 0.8-26.4 particles/m3 in fibers and fragments, respectively. Microplastics in the form of fragments are the most dominant form. The size of microplastics varies from 65 to 4,932 μm for fibers and 23 to 2,444 μm for fragments. These differences are due to primary and secondary microplastic degradation rates through weathering, abrasion, mechanical disintegration, photolysis, and microbiological activity. The colors of microplastics found were blue, brown, cream, red, black, and transparent, with transparent being the most dominant color. The material types found were polyethylene terephthalate (PET) and polypropylene (PP).

Enrichment characteristics of microplastics in Antarctic benthic and pelagic fish and krill near the Antarctic Peninsula

Global microplastic pollution has garnered widespread attention from researchers both domestically and internationally. However, compared to other regions worldwide, little is known about microplastic pollution in the marine ecosystems of the Antarctic region. This study investigated the abundance and characteristics of microplastics (MPs) in the gills and intestines of 15 species of Antarctic fish and Antarctic krill (Euphausia superba). The results indicate that the abundance of MPs in Antarctic fish and E. superba ranged from 0.625 to 2.0 items/individual and 0.17 to 0.27 items/individual, with mean abundances of 0.93 ± 0.96 items/individual and 0.23 ± 0.44 items/individual, respectively. Antarctic fish ingested significantly more MPs than E. superba. There was no significant difference in the abundance of MPs between the gills and intestines of Antarctic fish. However, the quantity of pellet-shaped MPs in the gills was significantly higher than in the intestines. The depth of fish habitat influenced the quantity and size of MPs in their bodies, with benthic fish ingesting significantly fewer MPs than pelagic fish. Pelagic fish ingested significantly more MPs sized 1–5 mm than benthic fish. Additionally, analysis of the characteristics of MPs revealed that fiber-shaped MPs were predominant in shape, with sizes generally smaller than 0.25 mm and 0.25–0.5 mm. The predominant colors of MPs were transparent, red, and black, while the main materials were polypropylene (PP), polystyrene (PS), polyamide (PA), and polyvinyl chloride (PVC). Compared to organisms from other regions, the levels of MPs in Antarctic fish and E. superba were relatively low. This study contributes to a better understanding of the extent of MP pollution in Antarctic fish and E. superba, aiding human efforts to mitigate its impact on the environment.

Effect of parasitic infection on microplastic ingestion in a native leuciscid hybrid species (Alburnus derjugini x Squalius orientalis) from Kürtün Dam Lake, Türkiye

Microplastic (MP) pollution is currently one of the most serious environmental issues. MPs were investigated in the Kürtün Dam Lake in healthy individuals of the native leuciscid hybrid (Alburnus derjugini x Squalius orientalis) species and individuals infected with the Ligula intestinalis parasite. Although MP abundance appeared to be higher in non-infected fish (NIF) than in L. intestinalis (L) and infected fish (IF), the MP abundance in IF was higher, because the parasite theoretically belongs to IF. In addition to the observation of MPs in the gastrointestinal tract (GIT) of fish, the diffusion of MPs by parasites settled in the body cavity indicates that MPs are not only present in the GIT. Therefore, predation on existing fish by birds causes MP dispersion. In the present study, the most common MP shape was fiber (100% for NIF and IF, 85.7% for L), the MP color was black (57.1% for IF and L) and orange (50% for NIF), and the polymer type was polyamide (57.1% for IF, 50% for NIF) and polyethylene terephthalate (28.5% for L). These MP compositions led us to believe that textile effluents and aquaculture operations in dam lakes could be sources of pollution. Therefore, this study provides insights for future research to elucidate the connection between MP consumption and parasite infection.

Barnacle analysis as a microplastic pollution bioindicator on the East Coast of Surabaya

Background Plastic pollution is a significant issue on the East Coast of Surabaya, emphasizing the need to develop microplastic monitoring programs. Barnacles became one of the potential microplastic bioindicator species on the East Coast of Surabaya. This study aimed to characterize the visual and polymers of microplastics found in barnacles and assess their potential as a bioindicator species for microplastic pollution on the East Coast of Surabaya. Methods Microplastic polymer analysis was performed using ATR-FTIR. Results A total of 196 microplastic particles were found in barnacles, water, and sediment. The size of microplastics in barnacles, water, and sediment varied, with the size in barnacles dominated by class 1 (1–10 µm), in water by class 2 (10–50 µm), and in sediments by class 3 (50–100 µm). Fragments dominated the shape of microplastics in barnacles, while water and sediment were dominated by fiber. The microplastic color in barnacles, water, and sediment was dominated by blue, and the microplastic polymer composition on barnacles, water, and sediments was dominated by cellophane (36%). Amphibalanus amphitrite was found to be predominant and identified as a potential microplastic bioindicator because it is a cosmopolitan species. Its population was found to correlate positively with cellophane (CP) accumulation. The Pearson’s correlation test between barnacle length and microplastic length at a = 0.05 was inversely proportional to r = − 0.411 (p &lt; 0.05), categorized as a strong enough correlation. These findings are essential in developing monitoring programs and mitigating the impact of microplastics on the marine environment.

Microplastic pollution responses to spatial and seasonal variations and water level management in a polymictic tropical reservoir (São Paulo, Brazil).

We assessed microplastic (μP) pollution in water and sediment samples during the dry and rainy season (October/2018 and March/2019, respectively) from the Guarapiranga Reservoir in the Metropolitan Region of São Paulo, Brazil, which provides drinking water for up to 5.2 million people. The concentration of mPs varied spatially and seasonally, with the higher concentrations observed near the urbanized areas and during the dry season. Water column concentrations ranged from 150 to 3100 particles/m3 and 0.07-25.05 mm3 plastic/m3 water during the dry season, and 70-7900 particles/m3 and 0.06-4.57 mm3 plastic/m3 water during the rainy season. Sediment samples were collected only during the rainy season, with concentrations ranging from 210 to 22,999 particles/kg dry weight and 0.15-111.46 mm3/kg dry weight. The particle size distribution exhibited seasonal variation, with μPs >1 mm predominating during the dry season, constituting 60-75% of all particles. In terms of quantity, fibers accounted for the majority of microplastics, comprising 55-95% during the dry season and 70-92% during the rainy season. However, when considering particle volume, irregular particles dominated in some samples, accounting for up to 95%of the total amount. The predominant colors of microplastics were white/crystal, black, and blue, with the main compositions identified as polypropylene (PP) and polyethylene terephthalate (PET), suggesting the influence of untreated domesticsewage discharge. Additionally, some additives were detected, including the pigments Fast RED ITR and phthalocyanine blue. The management of reservoir water levels appears to influence the quantity of μPs in the water column. As the water level increases up to 90% of the reservoir capacity during the rainy season, the amount of μPs in the water decreases, despite the higher influx of particles resulting from surfacerunoffcaused by rainy conditions. This suggests a "dilution" effect combined to the polymictic mixing hydrodynamics. Our results may contribute to the creation and improvement of monitoring programs regarding mP pollution and to the adoption of specific public policies, which are still lacking in legislation.

Microplastic accumulation, morpho-polymer characterization, and dietary exposure in urban tap water of a developing nation

The recent detection of microplastics (MPs) in a large number of commercially important food items and beverages, including tap water, has drawn significant attention because of direct exposure and negative health effects on humans. Nevertheless, there is insufficient information on microplastic contamination in the tap water of developing countries. In the present study, we primarily analyzed supplied tap water samples from four major cities in Bangladesh to determine and characterize MPs using a stereomicroscope and Fourier transform infrared spectroscopy (FTIR). Several indices were employed to calculate human health exposures to microplastics. MPs were found in all of the water samples tested, with an overall mean of 35.33 ± 19.55 particles/L. The results of this study diverge from those of comparable research conducted globally, revealing that tap water in Bangladesh exhibited higher levels of contamination compared to other nations. MPs were found in three different shapes (fibers, fragments, and films), with fibers dominating the samples (96.2%), and 98.1% of the microplastics were less than 0.5 mm in size. Six different colors of MPs were observed, and transparent particles were dominant (63.9% of all observed MPs). FTIR infrared spectrum analysis revealed two major types of polymers: low-density polyethylene (LDPE) and high-density polyethylene (HDPE). The projected daily consumption of microplastics was determined to be 2.65 particles per person per day, raising potential concerns for human health. The findings show that the treatment process of the water supply system is inadequate. Additionally, the sources of microplastics in tap water may come from where the water was collected for treatment and may be linked to a variety of anthropogenic activities, such as urbanization, sewage discharge, industrial waste disposal, and runoff from catchment areas.

Analysis of Microplastics on Digestive and Respiration System of Horse-Eye Jack Fish (Caranx latus) in Coastal Bungus Bay, West Sumatra

Microplastic pollution accumulates in the marine environment, thus impacting the health of marine organisms. The purpose of this study was to analyze and identify the abundance and characteristics of microplastics in Horse-Eye Jack fish (Caranx latus) in coastal Bungus Bay, West Sumatra. Fish samples were taken at three different locations, namely, Sako beach, Caroline Beach, and the east side of Bungus Bay. The average abundance of microplastics in horse-eye Jack fish samples in digestive is 21.63 ± 3.99 particles/fish, and in respiration, 9.23 ± 1.92 particles/fish. The shape identified forms of microplastics are fibers, films, fragments, granules, and foam, and the dominant color of microplastics is red. The most common size of microplastics found was &lt;0.5 mm. FT-IR spectroscopy analysis showed that the polymer types of microplastics found were PE, PVC, PET, and PS. Statistical tests showed differences between the abundance of microplastics in two pathways of microplastic uptake (digestive and respiration), and the sampling location P&lt;0.05. The results showed that microplastics were found in all Horse-Eye Jackfish samples in Bungus Bay, West Sumatra.These findings highlight microplastics' harm to the marine ecosystem and the necessity of managing plastic waste if we hope to avoid future plastic pollution catastrophes

Microplastic Content in Fish and Sea Water at Air Tawar Coast, Padang City, Indonesia

Every year, millions of tons of plastic contaminate marine ecosystems, disrupting the natural balance and endangering marine life. Marine debris, especially plastic, is exposed to sunlight and repeated physical activities in the ocean, eventually breaking down into microscopic particles known as microplastics. This research aims to identify the presence of microplastics in fish and seawater at Air Tawar Coast, Padang City, Indonesia, based on their shapes, colors, and sizes. Seawater samples were collected at 3 stations using purposive random sampling along the direction of net trawling. Water samples were collected using a plankton net and filtered horizontally at the sea surface. Fish samples were obtained from the catches of fishermen, using trawl net (maelo pukek). The fish sampled were Selar and Kembung fish. Microplastics found in seawater and fish exhibited various shapes, including fibers, films, fragments, and pellets, with pellets and granules being the most dominant shapes. The colors of microplastics found in this study were black, brown, red, orange, blue, yellow, green, and purple, with dark colors (black and brown) being the most dominant. The abundance of microplastics at station 1 was 140.12 particles/m3, at station 2 was 151.27 particles/m3, and at station 3 was 129.61 particles/m3. The number of microplastic particles found in Kembung was higher than in Selar. On average, Selar fish contained 228 particles/individual, while Kembung fish contained an average of 267.5 particles/individual. The smallest particle size was found in pellet form, with a size of 8.12 µm, while the largest particle size was found in film form, measuring 174.56 µm.

Global prevalence of microplastics in tap water systems: Abundance, characteristics, drivers and knowledge gaps

Tap water is a main route for human direct exposure to microplastics (MPs). This study recompiled baseline data from 34 countries to assess the current status and drivers of MP contamination in global tap water systems (TWS). It was shown that MPs were detected in 87 % of 1148 samples, suggesting the widespread occurrence of MPs in TWS. The detected concentrations of MPs spanned seven orders of magnitude and followed the linearized log-normal distribution (MSE = 0.035, R2 = 0.965), with cumulative concentrations at 5th, 50th and 95th percentiles of 0.028, 4.491 and 728.105 items/L, respectively. The morphological characteristics were further investigated, indicating that particles smaller than 50 μm dominated in global TWS, with fragment, polyester and transparent as the most common shape, composition and color of MPs, respectively. Subsequently, the SHapley Additive exPlanations (SHAP) algorithm was implemented to quantify the importance of variables affecting the MP abundance in global TWS, showing that the lower particle size limit was the most important variables. Subgroup analysis revealed that the concentration of MPs counted at the size limit of 1 μm was >20 times higher than that above 1 μm. Ultimately, current knowledge gaps and future research needs were elucidated.

DETECTION OF MICROPLASTICS IN BLOOD CLAM (Tegillarca granosa) AND GREEN MUSSEL (Perna viridis) FROM BERINGHARJO MARKET, YOGYAKARTA CITY

Plastic is one of the main pollutants in the ocean due to its persistent nature. Plastic in waters can be broken down into pieces &lt;5 mm in size, called microplastics, and enter the bodies of aquatic biota such as shellfish. The objectives of this research was to analyze the abundance and identify the characteristics of microplastics in blood clam and green mussel from Pasar Beringharjo, Yogyakarta City. The method in this research is to sample shellfish from Pasar Beringharjo. The shellfish samples obtained had their meat removed and soaked in 10% KOH for 3 days. The isolation results were filtered and the microplastics obtained were observed under a light microscope to determine their abundance and characteristics. The polymers that make up microplastics are analyzed by FTIR. The abundance and characteristics of microplastics between shellfish kinds were compared using the Mann Whitney test. The results showed that the abundance of microplastics in blood clam (average 839.25 ± 144.24 microplastics/individual) was higher compared to green mussel (average 703.75 ± 159.21 microplastics/individual), but not significantly different (p&gt;0.05). The form of microplastic that dominates in both kinds of shellfish is fragments. The most common color of microplastics found in both kinds of shellfish was black and brown. The size of microplastics in both kinds of shellfish is dominated by the range 0-100 µm. FTIR results indicate the type of nylon polymer (polyamide). Microplastics in blood clam and green mussel from Pasar Beringharjo, Yogyakarta City have almost the same abundance and characteristics.

Study on density, shape, color, and composition of microplastics in sediments at the Han River estuary (Da Nang)

Plastic pollution is recorded in terrestrial and aquatic ecosystems. Plastic waste exposed to the environment causes many problems for the environment and all forms of life. This study was conducted to evaluate the microplastic pollution in sediments at the Han River estuary (Da Nang). The analysis results at 8 survey locations showed that microplastics appeared in all samples and there was heterogeneity in density at 8 locations, ranging from 348.2÷3567.8 MPs/kg. Microplastic sizes in the range of 50÷150 μm account for the highest proportion with a value of 2531 MPs/kg, followed by sizes &gt; 500; 20÷50; 50÷150, and 300÷500 μm with ratio values of 2034.2; 1292.1; 842.1, and 595.9 MPs/kg, respectively. The fibers and fragment microplastic forms accounted for most sediment samples up to 98.6%, the white color of microplastic recorded an average value of 49.8%, accounting for nearly half of the microplastics total found. FTIR spectrum shows that PET (Polyethylene Terephthalate) polymer has the highest density with 38.7%; followed by Polyester 25.1%; Nylon 12.9%; Polypropylene 5.4%; remaining polymers such as PVC (polyvinylchloride), PS (polystyrene), PE (Polyethylene), HDPE (High Density Polyethylene), etc. account for a fairly low proportion ranging from 0.7÷2.7%. This source of microplastic pollution is closely related to civil and service activities on both sides of the river. Therefore, in addition to changing environmental management measures, it is nessesary to research to understand the mechanisms that affect the transport, deposition, and interactions of microplastics with organisms living in this sediment environment.

Impact of typhoon events on microplastic distribution in offshore sediments in Leizhou Peninsula of the South China Sea

Microplastic is an emerging pollutant and a technical fossil in Anthropocene sediments. Typhoon frequency and intensity have increased due to climate change, which has a major effect on the distribution patterns of microplastics. It is still unknown, though, how the topography of the peninsula affects the reconstruction of the distribution of microplastic in typhoons. Due to frequent typhoons, the Leizhou Peninsula (LZP) in the north part of the South China Sea is an ideal place to study the impact of topographic variations on microplastic distribution during typhoon events. This study investigated microplastics ranging in size from 50 μm to 5 mm in sediment. Microscopic inspection and μ-FTIR tests were used to identify microplastic characteristics from offshore surface sediments before and after typhoons. The average microplastic abundance in offshore sediments decreased from 18 ± 17 items/kg to 15 ± 15 items/kg after typhoons. Results show that typhoons only increase the microplastic abundance in topographically protected areas along the northeast coast of LZP, with no significant difference observed in other regions. The influence of typhoon on the morphological characteristics of microplastics in sediments is more pronounced and widespread, as evidenced by a shift in the predominant shape of microplastics from fibers to fragments and a decrease in size accompanied by an increased abundance within the 100 μm–1 mm fraction. The color of microplastics remained similar before and after typhoons, and the polymer composition of microplastics became more uniform. The alteration of microplastic morphology may be attributed to the enhancement of wave intensity induced by typhoons. This study enhances the comprehension of typhoon-induced impacts on pollutant redistribution, specifically microplastics, thereby providing essential empirical evidence and theoretical foundations for pollution regulation.

Assessment of microplastic contamination in some commercial fishes of the southern Caspian Sea and its potential risks.

This study examined the occurrence of microplastics (MPs) in the gastrointestinal tract (GIT) of 384 fishes classified into four species from 11 sites in 2022 from the southern part of the Caspian Sea. GITs of fishes were collected and digested in H2O2 and KOH at 45°C for 72h. After filtration, extracted MPs were observed under a stereomicroscope, and selected MP particles were identified using FTIR. Presence of MPs was 68.98% in the GIT of the investigated fish. The mean abundance of MPs was 5.9 ± 0.9 MPs/GIT in Rutilus kutum, 9.2 ± 1.2 MPs/GIT in Chelon auratus, 3.6 ± 0.7 MPs/GIT in Alosa braschnikowi, and 2.7 ± 0.5 MPs/GIT in Vimba vimba. The predominant form of MPs was fiber (58.21%), followed by fragment (34.77%). Black (34.4%), white (19.07%), and blue (14.58%) were the most frequently detected colors of MPs. Overall, 6 MP polymers were identified, dominantly polypropylene (42.86%), polystyrene (17.86%), and cellophane (14.28%). The western part of the Caspian Sea (mostly tourist spots and urban areas) showed more MP pollution in fish compared to the eastern part. Polymer hazard index (PHI) revealed alarming microplastic contamination in the southern Caspian Sea. The PHI value of the present study showed that PES (PHI = 8403.78) and PS (PHI = 535.80) were "Extreme danger" and "Danger" risk categories, respectively.

Ingestion and adherence of microplastics by estuarine mysid shrimp

Microplastics have been reported to be present in zooplankton, yet questions persist regarding their fate and dynamics within biota. We selected the commercial mysid shrimp, Mesopodopsis orientalis, as the focal zooplankton for this study due to their crucial role in our study area, the Inner Gulf of Thailand in January 2022. We investigated the presence of microplastics in mysid bodies and fecal pellets, examining both attached microplastics on external body parts and those ingested. In addition, we conducted microplastic feeding experiments, exposing mysids to various treatments of microplastics. The results of the field investigation indicate that mysids exhibited an average of 0.12 ± 0.03 microplastic items/mysid from whole-body samples. The shape, type, and color of microplastics found in mysids were similar to those present in seawater, with blue PET microfibers being the most prevalent. Our observations on live mysids revealed that microplastics were acquired through ingestion and adherence to appendages and exoskeletons. Microplastics were observed in mysid's fecal pellets at 0.09 ± 0.03 items/mysid, while microplastics adhering to the mysid's body and appendages were observed at 0.10 ± 0.04 items/mysid. The sizes of microplastics extracted from preserved mysids ranged from 58 μm to 4669 μm, with median of 507 μm. The laboratory experiments revealed that the presence of microalgae enhanced microplastic ingestion in mysids; microplastics incubated with a cyanobacterium, Oscillatoria sp., and diatom Navicula sp. significantly increased the number of microplastic particles ingested by mysids. This study showed that microplastics can be more ingested in mysids, especially when food items are present. Microplastic fate in these animals may involve expulsion into the environment or adherence, potentially facilitating their transfer up the marine food web.

Effect of Film Mulching Age and Organic Fertilizer Application on the Distribution Characteristics of Microplastics in the Soil of a Peanut Field

The large input of mulch film and organic fertilizer have led to increasingly serious microplastic pollution in farmland soil of China. In this study, the microplastic pollution of peanut farmland in Dezhou City, Shandong Province was investigated. The effects of different mulching years (0, 3, 5, and 8 years) and organic fertilizer application on the abundance, particle size, color, and shape of microplastics in farmland soil were analyzed. The results showed that the average abundances of microplastics in peanut soil were 65.33, 316.00, 1 098.67, and 1 346.34 n·kg-1, respectively, after 0, 3, 5, and 8 years of film mulching. The abundance of microplastics decreased with the increase in soil depth. The abundance of microplastics in 0-10, 10-20, and 20-30 cm topsoil was 1 076.00, 603.5, and 440.25 n·kg-1, respectively, and the abundance of microplastics increased significantly with increasing years of film mulching and organic fertilizer application (P<0.05). The particle size of microplastics in the sample plot <1 mm accounted for 77.30% of the total content, and with the increase in film mulching age, the proportion of microplastics with small particle size (<1 mm) increased significantly (P < 0.05). With the increase in soil depth, the proportion of microplastics with small particle size also gradually increased, whereas the application of organic fertilizer had no significant effect on the particle size of microplastics. The color of microplastics in the plot was mainly transparent (49.77%), followed by black (16.35%) and white (16.27%). The planting age and organic fertilizer application had no significant effect on the color of microplastics in the soil (P > 0.05), but the mulching age significantly increased the proportion of transparent microplastics. The abundance proportion of the five types of microplastics were 49.77%, 25.41%, 19.15%, 3.26%, and 2.41%, respectively. These field soil microplastics were mainly composed of polyethylene (PE), polypropylene (PP), and polystyrene (PS) polymers, accounting for 21.37%, 18.57%, and 19.77% of the total, respectively. Therefore, microplastics were widely present in the soil of the peanut field cultivated layer in Dezhou, Shandong, and the applications of mulch film and organic fertilizer were the main source. This study provides an important basis for the prevention and control of soil microplastic pollution in peanut fields.

Microplastic study on (litopenaeus vannamei) cultivation enterprises on the east coast of aceh

Microplastics are small plastics measuring &lt;5mm which, if they enter the water, can contaminate aquatic biota such as shrimp. The research aims to identify the number, shape and color of microplastics in shrimp bodies in vanname shrimp cultivation businesses on the East Coast of Aceh. Shrimp samples were taken from 3 stations, namely Pidie, Pidie Jaya and Bireuen. The samples were measured for weight and length, then prepared using H2O2 and H2SO4 in a ratio of 3:1 20 mL then incubated, heated, then filtered and identified using a Zeiss microscope with 40x magnification. The results obtained from shrimp body samples contained 2 types of microplastics, namely fiber and film. The most dominant microplastics at the three stations were 13 particles in the Pidie location, 9 particles in the Pidie Jaya location and 5 microplastic particles in the Bireuen location. The presence of microplastics that have entered the body of biota in the vannamei shrimp cultivation business is an early warning. So it is necessary to develop research on other consumption biota to determine the presence of microplastics and their impact if consumed by the public.