Color Change Index Research Articles

Dielectric Barrier Discharge Cold Plasma Improves Storage Stability in Paddy Rice by Activating the Phenylpropanoid Biosynthesis Pathway.

A nonthermal pretreatment using dielectric barrier discharge cold plasma (DBD-CP) was developed to improve the stress resistance of paddy rice during postharvest storage. The physicochemical properties, bioactive characteristics, and secondary metabolites of paddy rice were assessed after applying an optimized DBD-CP procedure, with enzyme activities and gene expression monitored over a 60 day storage period at 35 °C. A 17.06% reduction in the total color change index was noted in the DBD-CP group. Bioactive compounds, particularly gallic acid, were significantly increased, enhancing the defense mechanisms against high-temperature stress. Nontargeted metabolomics analysis indicated an upregulation of phenylpropanoid metabolism in DBD-CP-treated rice compared to controls, with notable increases in secondary metabolites such as coumaric acid, caffeic acid, and sinapic acid, suggesting potential biomarkers for stress resistance. Further verification showed significant enhancements in key enzymes of phenylpropanoid metabolism, including phenylalanine ammonia lyase (PAL), cinnamic acid-4-hydroxylase (C4H), plant coumaric acid-3-hydroxylase (C3H), and cinnamyl alcohol dehydrogenase (CAD), with increases ranging from 1.71 to 2.28 times. Gene expression levels of OsPAL7, OsC4H4, and OsCAD2 aligned with these enzymatic changes post-DBD-CP treatment. In conclusion, DBD-CP treatment can modulate phenylpropanoid metabolism in paddy rice, thereby enhancing bioactive compound levels to reduce stress damage during high-temperature storage.

GABA primes defense responses against Botrytis cinerea in tomato fruit by modulating ethylene and JA signaling pathways

This research set out to investigate the potential action mechanism of γ-aminobutyric acid (GABA) in the control of Botrytis cinerea (B. cinerea) in tomato fruit. The findings confirmed that 10 mM GABA treatment effectively lowered the disease incidence and lesion diameter of tomato fruit caused by B. cinerea. Meanwhile, GABA treatment maintained lower electrical conductivity, color change index and lycopene content. GABA alone had little effect on most disease resistance indexes, but it triggered faster and stronger defense responses after B. cinerea infection. These responses included increases in total phenolics and flavonoids accumulation as well as the activities of chitinase (CHI), β-1,3-glucanase (GLU), phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), and up-regulated expression of the pathogenesis-related genes (SlPR1, SlPR2a, SlPR2b, SlPR3a, SlPR3b and SlPR-STH2). Furthermore, GABA inhibited the expression of key synthesis genes of ethylene (SlACS2, SlACS4 and SlACO1) and the accumulation of a key metabolic intermediate 1-Aminocyclo-propane-l-carboxylic acid (ACC) in non-infected fruit, leading to a slight reduction in ethylene production. However, it also reduced the ethylene burst carried on by B. cinerea infection at the early storage and restored the ethylene peak at the end of storage. Furthermore, GABA up-regulated the expression level of SlERF6 directly but induced the up-regulation of SlERF2, SlERF.A4, SlERF.B12, SlERF.C6 and SlERF.H9 in a primed manner. In addition, GABA pretreatment markedly enhanced jasmonic acid (JA) content by activating the transcription of JA biosynthetic genes (SlLoxD, SlAOC and SlOPR3), up-regulated the downstream receptor gene SlCOI1 and down-regulated the suppressor gene SlJAZ2. These results demonstrated that tomato fruit treated with GABA can efficiently enhance resistance against B. cinerea through defense priming, and ethylene and JA signaling pathways are involved in this process.

Advanced coatings for consolidation of pottery artifacts against deterioration

After being buried in the soil for long periods exceeding thousands of years, archeological pottery showed many phases of deterioration leaving them with extreme impairment. Polymeric coatings are the traditional consolidants which can be used in the improvement of many of these discovered artifacts. Many of the used polymers can offer good protection performance, but this is accompanied with some problems; such as low stability with chromatic changes and wetting-drying cycles. The process of reinforcing the polymeric coating with functional pigments can enhance their performance in providing advanced functions to the consolidated coatings. In this work, polymers of styrene acrylic and VEOVA were integrated with inorganic pigment of strontium aluminate doped with traces of cerium to add-valued properties to the polymeric mixture and provide modified mechanical and anti-microbiological protection for the pottery artifacts. Different techniques were used to evaluate the effect of the prepared formulations such as their color change index, porosity, compressive strength, digital and scanning microscope besides the antimicrobial effects. The results showed that styrene-acrylic polymer integrated with the prepared strontium aluminate pigment was better in its protection and consolidating properties than that of VEOVA.

Experimental Research to Determine the Effect of Ultrasound in Drying Bo Chinh Ginseng by Ultrasound-Assisted Heat Pump Drying Method

This study focused on the experimental drying of Bo Chinh ginseng by the ultrasound-assisted heat pump drying method, in which the effects of drying air temperature (T), ultrasonic power (P), intermittency ratio of ultrasound generator (A) on the moisture effective diffusion coefficient (Deff), saponin content (Sp) and color change (dE) during the drying process were determined. The results showed that the drying time was reduced from 12.5 h at 35 °C to 8.9 h at 40 °C and greatly reduced to 5.8 h at 55 °C. The moisture diffusion coefficient Deff and color change index increased with the increase in drying temperature. Meanwhile, from 35 °C to 45 °C, the saponin content after drying tended to increase gradually, but when the temperature continued to rise from 45 °C to 55 °C, the saponin content decreased. The effect of ultrasonic power in the drying process was in particular as follows. The drying time of 8.8 h at 40 W ultrasonic power reduced to 7.8 h at 80 W ultrasonic power, and reduced to 7 h at 160 W ultrasonic power. The remaining saponin content after drying tended to increase gradually at the power level of 40–120 W (from 89.2% to 95.2%) and decrease when the power increased from 120 to 160 W (from 95.2% to 90.5%). The moisture diffusion coefficient Deff also increased with the increase in ultrasonic power. Meanwhile, the color change of dried products decreased as the ultrasonic power increased in the range of 40–120 W but increased with the ultrasonic power range from 120 to 160 W. Additionally, the experimental method and Box–Behnken design were used for optimizing the drying process with the optimal drying conditions such as drying air temperature of 45.2 °C, ultrasonic power of 127.7 W and intermittency ratio of ultrasound generator of 0.18.

Optimization of biodegradable paper cup packaging coated with whey protein isolate and rice bran wax as potential popcorn package.

Biodegradable paper cups coated with rice bran wax and whey protein isolate were designed to package popcorn. Coatings with different concentrations of whey protein isolate (5.5, 7.75, and 10% w/v) and rice bran wax (0.2, 0.4, and 0.6% w/v) were applied on the outer surface of the paper cups. Thickness, color changes, Young's modulus and tensile strength, water vapor transmission rate (WVTR) of the coated and uncoated cups, and also popcorns properties (pH, texture, and sensory properties) were evaluated. Water vapor transmission rate, Young's modulus, thickness, total color change index, and tensile strength of coated cups with the optimal coating formulation was 19.785 (g/m2 day), 11.810 (MPa), 276.583 (µm), 1.839, and 11.222 (MPa), respectively. The results showed that paper cup coating increased thickness and yellowness and reduced the brightness, Young's modulus, and WVTR. Coating had a positive effect on the pH and texture of popcorns packaged in coated cups than samples packed in uncoated cups (p < .05). With increasing storage time due to moisture absorption, popcorn changes from crisp to viscoelastic and increases tissue firmness (p < .05). Popcorns’ taste in uncoated cups had gained significantly lower scores by panelists compared with the samples packed in coated cups. There was a significant decrease in the general acceptance of popcorn during storage and also the type of coating used in the packaging cup (p < .05). Storage time and type of coating showed no significant effect on the moisture content, odor, and appearance of popcorn. In sensory evaluation, the coated packaging increased the taste, no difference in odor and appearance, and increased the overall acceptance of popcorn compared to the sample in uncoated cups. In general, the results showed that paper cup coating could be a new approach for barrier property improvement in paper‐based food packaging and extending the shelf life of the products.

Image Analysis to Quantify Weight-length, Weight-area, and Change of Color of Three Commercial Mullidae Species during Cold Storage

ABSTRACT Image analysis was used to quantify weight-length, weight-area, and change of color for three fish caught in the Mediterranean (Red mullet Mullus barbatus, Por’s goatfish Upeneus pori, and goldband goatfish Upeneus moluccensis) during 7 days of storage at 4 ± 1°C. Length-weight data were fitted with a power equation. R2 ranged from 0.759 to 0.880. U. moluccensis showed an isometric growth, while the others had a negative allometric growth. For the view area (V)-weight relationship, linear and second order polynomial equations were also used in addition to the power equation. The R2 values for these ranged from 0.895 to 0.952. Average L*, b*, and ΔE values for all fish increased with storage time; but the a* values decreased (P < .05). The color change index (CCI) of the fish over storage time decreased, suggesting a smoothing of colors. Although its colors changed over time, the yellow band of the goldband goatfish did not change. Correlation of these computer-generated parameters using image analysis would allow quantitative evaluation of fish color with sensory analysis.

Effect of pretreatment on physicochemical, microbiological, and aflatoxin quality of solar sliced dried ginger (Zingiber officinale Roscoe) rhizome.

Pretreatment of fruit and vegetables is necessary to reduce microbial proliferation and to preserve color of the produce. The effect of drying and pretreatment with potassium metabisulfite (KMBS) of concentrations 0.0%, 0.1%, 0.15%, 0.2%, and 1.0% and blanching at 100°C and 50°C using a tent‐like concrete solar (CSD) dryer as compared to open‐sun drying (OSD) of yellow ginger rhizomes was investigated using routine methods. The total color change and residual sulfur dioxide (SO2) were analyzed. KMBS reduced the yeast and mould load significantly from 3.6 × 104 ± 1.4 × 103 CFU/g in 0.0% (control) to <10 CFU/g in 1.0% KMBS and 100°C blanched fresh samples. Drying of the fresh samples for 5 days increased the yeast and mould load of all the treatments to as high as 1.15 × 105 ± 2.12 × 104 CFU/g for the 1.0% KMBS. Overall, the CSD had fewer microbial loads than the OSD but it was not significant. Aflatoxins and Salmonella sp. were not detected in any of the samples. The sulfur dioxide residue (SO2) for KMBS pretreated samples increased as the concentration of KMBS increased with the CSD retaining slightly higher amount than the OSD. The total color change index increased with increase in KMBS, and drying further increased the total color change index. On the whole, the blanched samples had the least color change among the pretreatments with 100°C CSD showing the least change among the dried samples.

Comparative evaluation of coenzyme Q10-based gel and 0.8% hyaluronic acid gel in treatment of chronic periodontitis.

Background:The anti-inflammatory and immune enhancing effects of coenzyme Q10 (CoQ10) and hyaluronic acid are well established in medical literature. The present study was undertaken to evaluate their role in chronic periodontitis.Materials and Methods:One hundred twenty sites in 24 patients with clinically confirmed periodontitis were included in the study. A split-mouth design was used for intrasulcular application of CoQ10 as adjunct to scaling and root planing (SRP), 0.8% hyaluronic acid as adjunct to SRP and SRP alone. Clinical parameters such as plaque index (PI), gingival color change index (GCCI), Eastman interdental bleeding index (EIBI), pocket depth (PD), and clinical attachment level (CAL) were recorded. All the clinical parameters PI, EIBI, GCCI, PD, and CAL were recorded at baseline before SRP. Only PI, EIBI, and GCCI were recorded at 1st and 2nd week. Twenty-one days post 2nd week, i.e., 6th week all the clinical parameters were recorded again.Results:Intragroup analysis of all the clinical parameters showed clinical significant results between baseline and 6th week. However, on intergroup analysis, the results were not significant.Conclusion:The local application of CoQ10 and hyaluronic acid gel in conjunction with SRP may have a beneficial effect on periodontal health in patients with chronic periodontitis.

Effect of Accelerated Aging on the Color and Opacity of Resin Cements

The color stability of resin cements plays a major role in the esthetic performance of porcelain laminate veneers. Some dual-polymerizable resin cements used to bond porcelain laminates were shown to undergo color changes during service. Some recently produced cements are described as being color stable, but scientific data are not available. The current study evaluated the effect of accelerated aging on the color and opacity of resin cements. The hypothesis was that the auto-polymerizing cements would show less color and opacity stability. Forty (0.7 x 18 mm) feldspathic porcelain disks were prepared and divided into four equal groups. The resin cements were bonded to the disks by application of an identical load of 2.5 kilograms, and they were polymerized according to the manufacturer's instructions. The groups were: Variolink Veneer (light-polymerizing), Variolink II (light-polymerizing), Variolink II (dual-polymerizing) and Multilink (auto-polymerizing). A spectrophotometer was used to measure the following color parameters in the CIE L*a*b* color space on a black and white background: deltaa*, deltab*, deltaL*, deltaC, deltaH, deltaE and deltaCR (contrast ratio). The measurements were performed before and after aging. Paired t- and one-way ANOVA tests were used to analyze the data (alpha = .05). None of the groups showed significant differences in deltaE before and after aging (p > .05); deltaE remained in the range of clinical acceptance (deltaE < 3.3). All of the cements became more opaque, while deltaCR (difference in contrast ratio) was significantly different (p = .004). The auto-polymerized cement showed an increase in opacity. The studied cements behaved acceptably according to deltaE, but they became more opaque after aging. The studied cements can ensure color stability when used to cement porcelain laminate veneers, but the change in opacity can affect clinical results. Auto polymerizing cements become more opaque with aging; therefore, porcelain restorations may lose their match with other teeth.

Quantifying Nonhomogeneous Colors in Agricultural Materials Part I: Method Development

Measuring the color of food and agricultural materials using machine vision (MV) has advantages not available by other measurement methods such as subjective tests or use of color meters. The perception of consumers may be affected by the nonuniformity of colors. For relatively uniform colors, average color values similar to those given by color meters can be obtained by MV. For nonuniform colors, various image analysis methods (color blocks, contours, and "color change index"[CCI]) can be applied to images obtained by MV. The degree of nonuniformity can be quantified, depending on the level of detail desired. In this article, the development of the CCI concept is presented. For images with a wide range of hue values, the color blocks method quantifies well the nonhomogeneity of colors. For images with a narrow hue range, the CCI method is a better indicator of color nonhomogeneity.

Inhibition of Browning on Fresh-cut Pear Wedges by Natural Compounds

ABSTRACT: Mechanical operations such as peeling and cutting during minimal processing involve enzymatic browning of fruit tissue. The objective of this work was to evaluate the individual and combined effects of N-acetyl-L-cysteine, reduced glutathione, ascorbic acid, and 4-hexylresorcinol to control pear browning. Browning of fresh-cut pears was prevented by a minimum concentration of 0.75% N-acetyl-L-cysteine up to 28 d at 4°C. Reduced glutathione treatments were also effective along the storage time although browning was observed after 21 d of storage with a dip of 0.75% reduced glutathione. However, ascorbic acid or 4-hexylresorcinol treatments did not seem to completely prevent browning of pear wedges throughout the storage period. An enhanced antibrowning effect was observed when combining both N-acetyl-L-cysteine and reduced glutathione, considering hue angle as color change index. Thus, hue angle reached maximum levels at 1.5% N-acetyl-L-cysteine (NAC) or 1.5% glutathione (GSH) and 1% NAC with 1% GSH for 28 d. Besides, N-acetyl-L-cysteine, reduced glutathione and 4-hexylresorcinol completely inhibited polyphenol oxidase activity as well as browning inhibitors slightly reduced firmness of fresh-cut pears.

Drying and Color Characteristics of Coriander Foliage Using Convective Thin-Layer and Microwave Drying

Heat sensitive properties (aromatic, medicinal, color) provide herbs and spices with their high market value. In order to prevent extreme loss of heat sensitive properties when drying herbs, they are normally dried at low temperatures for longer periods of time to preserve these sensory properties. High energy consumption often results from drying herbs over a long period. Coriander (Coriandrum sativum L., Umbelliferae) was dehydrated in two different drying units (thin layer convection and microwave dryers) in order to compare the drying and final product quality (color) characteristics. Microwave drying of the coriander foliage was faster than convective drying. The entire drying process took place in the falling rate period for both microwave and convective dried samples. The drying rate for the microwave dried samples ranged from 42.3 to 48.2% db/min and that of the convective dried samples ranged from 7.1 to 12.5% db/min. The fresh sample color had the lowest L value at 26.83 with higher L values for all dried samples. The results show that convective thin layer dried coriander samples exhibited a significantly greater color change than microwave dried coriander samples. The color change index values for the microwave dried samples ranged from 2.67 to 3.27 and that of the convective dried samples varied from 4.59 to 6.58.