Collenchyma Tissue Research Articles

Türkiye'de Yetişen Beş Artemisia L. Türünün Gövde Ve Yaprak Anatomisinin İncelenmesia

In this study, it is aimed to make anatomical analysis of five different Artemisia L. species (A. absinthium L., A. annua L., A. abrotanum L., A. incana (L.) Druce, A. tournefortiana Rchb.) grown in Turkey and contribute to the definition of this genus, which has some taxonomic problems. The materials for anatomical study were preserved in 70% alcohol. Free hand cross sections were taken using sharp razor blades, stained and mounted in Sartur Reagent and Chloralhydrate (50%) solution. Anatomical structures of the stem and leaf of selected species were examined under the light microscope. Their detailed structures were illustrated with photographs. Results have shown that stem and leaf anatomical features of the Asteraceae family are mostly shared by all species. However, some characters could be considered species-specific. In cross- sections, the stems of all studied species have more or less irregular rounded shapes, but prominent ribs containing collenchyma tissue could be noticed only in A. annua stem. The leaves of A. absinthium and A. incana were covered by glandular and densely T-shaped non--glandular trichomes on both sides however, A. tournefortiana had no hair at all. There were highly apparent secretory canals located in both the stem (next to the endodermal cells) and the leaf (near to xylem) of A. tournefortiana. The anatomical structure of leaves and stems of A. tournefortiana and A. incana growing in Turkey was demonstrated for the first time. Our results revealed that the studied species are anatomically distinguishable from each other and also provided valuable features for better species identification and contribute to the anatomy of the genus Artemisia.

ANATOMICAL VARIATIONS OF LEAVES PETIOLES IN SOME TAXA OF THE GENUS TRIFOLIUM L. (FABACEAE) IN IRAQ.

This study was conducted on the 22 taxa of the genus Trifolium L. which grow naturally as wild plants in all geographical districts in Iraq. A comparative study of the leaf petioles was anatomically examined by paraffin method. Petiole cross-section outline shapes have divided the genus species into fourteen groups and sub-groups. According to the microscopical characteristics the cortex of petioles showed to contain layers of various tissues, the collenchyma tissue observed sub-epidermally in some species, and cortexes of the others were devoid of collenchyma. The sclerenchyma strands were noted in three patterns; above vascular bundles, above and beneath vascular bundles, and the sclerenchyma strands completely surrounded the vascular bundles in some species. Petiole cross-sections have been divided into three categories as; three vascular bundles and absent of accessory vascular bundles, three vascular bundles with one of accessory vascular bundle, three vascular bundles with two accessory vascular bundles. The comparative results confirmed that these characteristics collectively are of high taxonomic value and can contribute to separating the studied genus species, finally, based on these variations, the anatomical classification key was constructed to separate the genus taxa.

Microstructural Feature of Salvia verticillata L. vegetative and generative organs

It has been studied perspective medicinal plant Salvia verticillata L. aboveground parts vegetative and generative organs internal features.  The aboveground parts of the plant are intensively lined with conical, multicellular (2-7) and glandular spherical trichomes; In the covering tissue of the leaf blade, stalk, and stem the ventilation system is represented by diacytic type stomata. The leaf is bifacial, amphistomatic in terms of the location of the ventilation system, while the pulp is characterized by a dorsiventral structure. In the internal structure of Salvia verticillata aboveground parts coexistence of the plate type collenchyma, polygonal structure primary parenchyma, bast fibre and collenchyma tissue  are represented. Collateral-type vascular bundles reflected in the stem, leaf stalk, and midrib; The wood is differentiated into longitudinally arranged, spherically shaped vessels and tracheal elements, vascular tissue one -and two spiral, with porous tracheid wall thickening.

Comparative anatomy of flowering and sterile shoot leaf of Hedera helix L. (Araliaceae)

Hedera helix L. is a medicinal plant and belongs to the Araliaceae family. Flowering shoots of the plant have elliptic-cordate and sterile shoots have palmately lobed leaves. Plant material was collected from Hacıkadın valley (Ankara-Turkey). The anatomical features of leaf and petiole were examined comparatively in this study. The results showed that both leaf types were dorsiventral. Single layer epidermis is covered by thick cuticle. The palisade parenchyma cell is oval-shaped in the flowering shoot mesophyll, also is elongated in the sterile shoots. In both leaf types, stomata are located in the lower epidermis. Flowering shoot leaf is covered with tufted hairs, sterile shoot leaf stellate hairs. The midrib of the leaves have a similar structure. Collenchyma tissue is located underside the upper and lower epidermal layers in the midrib. The vascular bundles are arc-form and tends to a ring-form. The sclerenchymatous tissue surrounds the vascular bundles and secretory canals are located in the adaxial side of the bundles. The leaves contain abundant druse crystals and starch grains. Petiole anatomy is similar in both leaf types. Unlike leaves, petioles are covered with stellate hairs, do not contain tufted-type. The collenchymatous tissue is located bottom the epidermal layer. The vascular bundles are embedded in the parenchymatous tissue and secretory canals are located on the adaxial side of petiole. Both petioles contain numerous druse crystals and starch grains.

Phosphorus influence Cd phytoextraction in Populus stems via modulating xylem development, cell wall Cd storage and antioxidant defense.

The soils in mining lands with cadmium (Cd) contamination usually are deficient in nutrients. Disclosing how P nutrition and N:P stoichiometric ratio influences Cd accumulation and stress tolerance in stems of Populus spp. will facilitate the phytoremediation of mining sites polluted by Cd. In this study, investigations at the anatomical and physiological levels were conducted using a clone of Populus× euramericana. Both phosphorus deficiency and cadmium exposure inhibited xylem development via reducing cell layers in the xylem. Under P-sufficient condition, appropriate P status and balanced N:P ratio in stem promoted xylem development under Cd exposure via stimulating cell division, which enhanced Cd accumulation in stems. Cd accumulation in cell walls of collenchyma tissues of the stem was enhanced by P application due to increased polysaccharide production and cell wall affinity for Cd. The low P concentrations (0.3-0.4 mg g-1) and imbalanced N:P ratio under P deficiency inhibited the production of APX and ascorbate-GSH cycle, which increased oxidative stress and lipid peroxidation as indicated by high MDA concentration in stem. Under P-sufficient condition, the interactions between phytohormones and antioxidants play crucial roles in the process of antioxidant defense under Cd exposure. In conclusions, appropriate P addition and balanced N:P ratio enhanced secondary xylem development and promoted cadmium accumulation and stress tolerance in Populus stems, which can benefit the phytoextraction of Cd from Cd-contaminated soil.

PHARMACOGNOSTIC STANDARDIZATION OF JACARANDA MIMOSIFOLIA LEAVES AND STEM BARK

pharmacognostic standardization is a basic criterion to assure authenticity and quality of any medicinal plant. In the present study micromorphological examinations along with proximate analysis, fluorescence and elemental analysis of Jacaranda mimosifolia leaves and stem bark were carried out. Morphological examination indicated compound, bipinnate and alternately arranged leaves having several pairs of leaflets. The leaflets were oblong ellipsoidal in shape with parallel venation, acute apex and entire margin. The stem bark was concavely curved, greyish brown colored with conical shaped buds on the surface. Microscopic examination of leaves presented dorsiventral lamina, unicellular pointed trichomes, vascular bundle, collenchyma tissues and calcium oxalate crystals while bark indicated cork, stone cells, lignified fibres, calcium oxalate prisms, starch grains and medullary rays. Elemental analysis revealed the presence of aluminum, chlorides and iron in leaves and bark of Jacaranda mimosifolia. The present study will assist as an important source of information in identification and further investigation of this plant.

Taxonomic Significance of Anatomical Characters in Some Species of Brassicaceae family in Iraq

This paper elaborated transverse sections of leaves and stems of nineteen species belongs to 18 genera of Brassicaceae family. Anatomical characteristics were important in separation of taxa. The presence or absence of Collenchyma, Chlorenchyma and Pith, Mesophyll structure and epidermal surface were found to be important characters for the identification of Brassicaceae species. Two types of mesophyll have been recognized, dorsiventral (Bifacial) found in Sinaps alba L., Biscutella didyma L., Sameraria stylophora(Jaub. et Sp.) Boiss., Drabopsis nuda (Bel.) Stapf., Eruca sativa Mill., Capsella bursa-pastoris (L.) Medic., Calepina irregularis(Asso)Thell., Diplotaxis harra (Forssk.) Boiss., Sinapis arvensis L. and Nasturtium officinale R.Br. and isobilateral in the remaining species. Transverse sections of the midrib showed that most of species contains one vascular plants in all the species except Brassica oleracea L., Cheiranthus cheiri L., Crambe orientalis L., Draba bruniifolia Stev. and Sinaps alba L. which contains (1-3) vascular bundles. Collenchyma tissue present only in Diplotaxis harra, sinaps alba, Drabopsis nuda (Bel.) Stapf. Camelina rumelica, Cramba orientalis L., Cheiranthus cheiri L. and Cardaria drab Thell, it was higher in Cardaria drab was (150.50) um and lower in Crambe orientalis was (18.75) um. A diagnostic key based on combined stem and leaf were presented.

Evaluation of Mineral Profile, Texture, Sensory and Structural Characteristics of Old Pepper Landraces

Old traditional Serbian pepper landraces (Nizača, Lokošnička and Turšijara), grown in South Serbia and produced by seed sowing or transplanting, were evaluated in this study. Besides the basic morphological properties and composition, the features characterizing fruit color and pericarp anatomy were determined in order to analyze and systematize the traits relevant from the aspect of traditional end use of examined landraces. Landraces Lokošnička and Nizača, characterized with higher force needed to puncture fruit skin, larger number of mesocarp layers, thicker endocarp and thicker collenchyma tissue, followed with higher total soluble solids content are more suitable for intended traditional use than Turšijara. Production from transplants should be favored in order to obtain more uniform, darker red fruit color, ASTA value, higher TSS content as well as higher yields. There are genetic differences of the some quality differences between the cultivars. Lokošnička has higher mesocarp layers, thicker endocarp and collenchyma tissue. Practical Applications Pepper fruits dried in traditional way slowly in a draught are used in preparation of local ethno-gastronomic specialities, with special position in local customs related to dietary habits during the spring Orthodox fasting period. Intact fruit skin is also the prerequisite for successful utilization in traditional culinary specialties-stuffed dry pepper fruits previously soaked in water that remain whole during cooking and baking. Structure and texture of pepper fruit skin and pericarp can consequently be considered as one of the key aspects of technological quality of pepper based on which landraces were selected for described traditional preservation technique. The research for answers to this technological challenge was the motivation of this study, which evaluated the impact of different predrying process (type of landraces, production methods) on qualities of pepper fruit. The data shown would provide scientific rationales for obtaining high-quality whole fruit pepper as material for stuffing.

Morpho-anatomical study of rhizome of Limonium brasiliense

Limonium brasiliense (Boiss.) Kuntze, Plumbaginaceae, is an herb popularly known as guaicuru, guaicurá or baicuru. The species inhabits salt marshes from the coastal region of southern Brazil, including Rio de Janeiro, to Uruguay and Argentina. Although widely used in folk medicine in the state of Rio Grande do Sul to treat genitourinary infections and to regulate menstrual periods, L. brasiliense has been little studied. The present morpho-anatomical study was undertaken to resolve some doubts in the literature as to the nature of the part of the plant that is used for medicinal purposes, a true rhizome or a root. The morpho-anatomical characteristics were analyzed with the aid of light and scanning electron microscopy. The botanical material was characterized as a rhizome with internodes that are evident in the younger but not the older portions. Microscopic analysis revealed the presence of a multilayered periderm with a cortex, ray parenchyma, and pith, formed by collenchyma tissue with abundant intercellular spaces in the outer portions of the cortex, responsible for the rigidity of the body, and with walls impregnated with phenolic compounds. The vascular bundles are collateral with elliptical to elongated cells, and with few conducting and sclerenchymal elements. Groups of sclereids are dispersed through the cortex and pith. These morpho-anatomical characteristics define the structure as a rhizome.

Ethyl methane sulfonate induced mutations in M2 generation and physiological variations in M1 generation of peppers (Capsicum annuum L.).

This study was conducted to enhance genetic variability in peppers (Capsicum annuum, cv B12) using ethyl methanesulphonate (EMS). Exposure to an EMS concentration of 0.6%, v/v for 12 h was used to mutagenize 2000 seeds for the first generation (M1). It was observed that the growth behaviors including plant height, flowering date, and number of seeds per first fruit were different in the M1 generation than in wild type (WT) plants. In addition one phenotypic mutation (leaf shape and plant architecture) was observed during the M1 generation. During the seedling stage in the M2 generation, the observed changes were in the form of slow growth or chlorophyll defect (e.g., albino, pale green, and yellow seedlings). At maturity, there were three kinds of phenotypic mutations observed in three different families of the mutant population. The first observed change was a plant with yellow leaf color, and the leaves of this mutant plant contained 62.19% less chlorophyll a and 64.06% less chlorophyll b as compared to the wild-type. The second mutation resulted in one dwarf plant with a very short stature (6 cm), compact internodes and the leaves and stem were rough and thick. The third type of mutation occurred in four plants and resulted in the leaves of these plants being very thick and longer than those of WT plants. Furthermore, anatomical observations of the leaf blade section of this mutant plant type contained more xylem and collenchyma tissue in the leaf midrib of the mutant plant than WT. In addition, its leaf blade contained thicker palisade and spongy tissue than the WT.

Microscopic aspects of the colonization of Pyricularia oryzae on the rachis of wheat plants supplied with silicon

Considering the importance of blast, caused by Pyricularia oryzae, to reduce wheat yield, this study investigate how silicon (Si) could reduce the wheat blast symptoms in the rachis tissues using light microscopy and scanning electron microscopy. Wheat plants (cv. BR 18) were grown in hydroponic culture with either 0 (–Si) or 2 mM (+Si) of Si. Blast symptoms were very well developed on the spikes of the –Si plants, which showed intense discoloration in contrast with the spikes of the +Si plants. At 72 hours after inoculation (hai), fungal hyphae extensively colonized the epidermis and the collenchyma tissue in the radial direction in the rachis of the –Si plants. In the +Si plants, fungal hyphae colonized the epidermis and the collenchyma cells to a lesser extent than in the –Si plants. At 96 hai, fungal hyphae were observed in the epidermis, vascular bundles and cortical tissue in the rachis node of the -Si plants. In the +Si plants, a phenolic-like material was detected in the parenchyma with lower fungal colonization in comparison with the –Si plants. In scanning electron microscopy, fungal hyphae were scarcely observed in the upper epidermal, collenchyma and parenchyma cells in the rachis tissue of the +Si plants, whereas in the rachis tissue of the –Si plants, fungal hyphae extensively colonized the epidermis, collenchyma, parenchyma and vascular bundles.

Key leaf traits indicative of photosynthetic plasticity in tropical tree species

The main achievement of this work was an attempt to determine the best association between five tropical species and light environments, based on the physiological and morphological characters related to photosynthesis. Information about light tolerance and photosynthetic plasticity of indigenous tropical tree species is still limited, particularly information of first years of plants’ development. In this study, we evaluated the adjustments in response to different light environments of 25 leaf traits most commonly assessed in studies on light acclimation of photosynthesis in current literature. This evaluation was used to investigate the photosynthetic plasticity on young plants of five tropical tree species belonged to different successional groups. All the species are commonly used in forest restoration programs in Brazil. Plants were grown for 6 months under different light conditions simulating environments that could exist due to variation in naturally occurring canopy openings of secondary tropical forests. The level of adjustment on leaf traits to environmental conditions was calculated via a plasticity index. The relation between leaf trait adjustments and species photosynthetic plasticity was investigated by multivariate Biplot analyses. We selected the seven most explicative leaf traits of the photosynthetic plasticity of the studied species in response to different light environments: dark respiration rate (R d), Rubisco carboxylation capacity (V cmax), total chlorophyll content (ChlT), contribution of spongy parenchyma (%SP), contribution of leaf collenchyma tissue (%C), chlorophyll parenchyma thickness (PP/SP) and specific leaf area (SLA). Based on the selected traits, we identified the traits most related to high plasticity (V cmax, PP/SP, %SP, %C and SLA) and low plasticity (V cmax, R d and ChlT) and grouped species into three different patterns of photosynthetic plasticity. Our plasticity grouping was not correlated with species successional classification, indicating the importance of including physiological features related to light tolerance in species successional classifications. This work provides complementing information to traditional species successional groupings and to our current ability to select species for enrichment planting on restoration efforts.

ANATOMICAL AND COMPARATIVE STUDY OF SELECTED WILD SPECIES FROM THE FAMILY COMPOSITAE IN ANBAR

Wild Species of seven Iraqi Asteraceae family were compared anatomically these specie Sonchus oleraceus, Silybum marianum, Gymnarrhena micrantha ,Calendula persica, Aster subulatus, Taraxucum officinale and Onopordon canum include Detailed of anatomical of stem, petiole and roots .The result showed that anatomical characters of stem and petiole such as shape outline, number of vascular bundles, size of pith and cortex, present of Chlorenchyma and collenchyma tissue or no are of considerable taxonomic value at the specific or generic level and can be used for taxa separation. The study showed the characters of epidermis , cortex' and vascular Bundal forms is be made the center of root and the measure the xylem and phloem .,

Anatomical Study and Pollen Micromorphology of <i>Onopordum</i> L. in Iran

In this study, pollen micromorphological structure, leaf and stem anatomy structure of six species of Onopordum (O. acanthium L., O. armenum Grossh., O. carduchorum Bornm. & Beauv., O. carmanicum (Bornm.) Bornm., O. heteracanthum C. A. Mey and O. leptolepis DC) were examined with different repetitions. About 17 qualitative anatomical features were statistically analyzed. Glandular trichome, cortex fiber tissue, embowed in subsidiary vascular, its fiber tissue and vascular sheath extensions, adaxial collenchyma tissue and types of adaxial and abaxial stomata were distinct characters in separating of the species. In palynologic studies based on P/E ratio, 2 types of pollen grains were observed: oblate- spheroidal and suboblate. SEM study of the exine showed echinate, reticulate and perforate sculpturing in all six species.

Anatomical Study and Pollen Micromorphology of <i>Onopordum</i> L. in Iran

In this study, pollen micromorphological structure, leaf and stem anatomy structure of six species of Onopordum (O. acanthium L., O. armenum Grossh., O. carduchorum Bornm. & Beauv., O. carmanicum (Bornm.) Bornm., O. heteracanthum C. A. Mey and O. leptolepis DC) were examined with different repetitions. About 17 qualitative anatomical features were statistically analyzed. Glandular trichome, cortex fiber tissue, embowed in subsidiary vascular, its fiber tissue and vascular sheath extensions, adaxial collenchyma tissue and types of adaxial and abaxial stomata were distinct characters in separating of the species. In palynologic studies based on P/E ratio, 2 types of pollen grains were observed: oblate- spheroidal and suboblate. SEM study of the exine showed echinate, reticulate and perforate sculpturing in all six species.

Chloroplasts in tissues of some herbaceous stems

Serial sections of mature stems of ten species of herbaceous dicotyledonous plants were examined by light microscopy and the number of chloroplasts per cell was estimated in epidermis, collenchyma and cortex. Chloroplast identification was made by both light and transmission electron microscopy. Chloroplasts were present in epidermis, collenchyma and cortex tissues of all stems examined. The smallest number of chloroplasts was observed in the epidermis. Collenchyma cells had the largest number of plastids in four of the genera and cortex cells had the largest number in the remaining six genera. The stem epidermis of all genera contained stomates as demonstrated by scanning electron microscopy and aceto-orcein stained epidermal peels.

Microstructure and histochemical localization of flavonoids in leaves and stem in Sarcandra glabra

Microscopic and histochemical methods were used to investigate flavonoids localization in the leaf and the stem of the Sarcandra glabra. The results indicated that flavonoids distributed mainly in epidermis, collenchyma, vascular bundles, secretory cells and palisade tissue of leaf. In the stem, they distributed mainly in epidermis, collenchyma, phloem and secretory cells. Histochemical localization of flavonoids using 5% solution of NaOH is convenient, rapid and reliable. The content of flavonoids in the leaf was higher those than in the stem. For sustainable utilization of the resources we suggested that only the leaves could be harvested.

Differences in mechanical and structural properties of surface and aerial petioles of the aquatic plant Nymphaea odorata subsp. tuberosa (Nymphaeaceae)

Lily pads (Nymphaea odorata) exhibit heterophylly where a single plant may have leaves that are submerged, floating, or above (aerial) the surface of the water. Lily pads are placed in a unique situation because each leaf form is exposed to a distinctly different set of mechanical demands. While surface petioles may be loaded in tension under conditions of wind or waves, aerial petioles are loaded in compression because they must support the weight of the lamina. Using standard techniques, we compared the mechanical and morphological properties of both surface and aerial leaf petioles. Structural stiffness (EI) and the second moment of area (I) were higher in aerial petioles, although we detected no differences in other mechanical values (elastic modulus [E], extension ratio, and breaking strength). Morphologically, aerial petioles had a thicker rind, with increased collenchyma tissue and sclereid cell frequency. Aerial petioles also had a larger cross-sectional area and were more elliptical. Thus, subtle changes in the distribution of materials, rather than differences in their makeup, differentiate petiole forms. We suggest that the growth of aerial petioles may be an adaptive response to shading, allowing aerial leaves to rise above a crowded water surface.

Morphological and anatomical characteristics of the root and herb from Eryngium campestre L. (Apiaceae)

Research on the morphological and anatomical constitution of the root and herb from the plant Eryngium campestre L., Apiaceae has been done in this work. The follow elements important for the identification of drug have been defined: in leafs thick cuticle, isolateral mesophill with palisade tissue on the upper and lower region of the leaf and much reduced spongy parenchyma; characteristic arrangement of the mechanical collenchyma tissue, especially in leafs, characteristic wrinkles in the leaf cuticle. In the herb's powder the follow elements could be found: trachea fragments with specific ring wrinkles, parts from the epiderma with stomata, druses and knee-shaped mechanical hairs. In the root and the stem characteristic are secretory tubes and the presence of calcium oxalate druses in great quantity. Additionally in the root typical arrangement of tissues characteristic for the secondary root construction could be noticed, with: xylem rays in wood and in a bark, laticifers, periderma on the periphery, characteristic arrangement of the vascular bundles and great number of druses, esspecialy in the wood. In the root powder: trachea fragments with spiral thickenings, fragments of periderm and cork, parts from parenchyma of the bark with druses could been put aside.

Anatomical relations of the leaves in strawberry

In the present study histology of the leaves of strawberry (Fragaria ananassa Duch.) variety Elsanta was the objective, which has been performed with the beginning of seedling stage, cotyledons, primary leaves and later true leaves, first cataphyll of the runner shoot as well as the bracteoles of the inflorescence. Structures of the leaf blade, the upper and lower epidermis, the petiole have been also observed. The leaf blade of cotyledons already contains a typical palisade as well as spongy parenchyma tissues, i.e. being bifacial showing a structure similar to that of the true leaf. However, the petiole displays differences from the true leaf. There are a narrow (4-5 layer) primary cortex and a tiny central cylinder. Primary leaves bear already hairs on the adaxial surface and the transporting tissue-bundles are recognised in cross sections having a "V" shape. The first true leaf composed by three leaflets is of a simple structure showing characters reminding of cotyledons and primary leaves. Leaves of intermediate size continue to grow, whereas their inner anatomy changes dramatically. In the central region of the leaflets, near to the main vein, a second palisade parenchyma appears, further on, transporting tissue bundles are branching in the petiole. Collenchyma tissues enhance the stiffness and elasticity of the petiole. Older true leaves develop thick collenchyma tissues around the transporting bundles being represented by increasing numbers. The doubled palisade parenchyma layers of the leaf blades are generally observed. The cataphylls of the runners have a more simple structure, their mesophyll is homogenous, no palisade parenchyma appears. It is evident that leaves grown at successive developmental stages are different not only in their morphological but also anatomical structure. There is a gradual change according to the developmental stage of the leaves.