In modern society, alongside the rapid advancement of science and technology, the role and importance of mathematics are increasingly being emphasized. Such advancements, including data analysis and artificial intelligence development, rely fundamentally on mathematical thinking and theory, which have become essential elements across all academic disciplines. Against this backdrop, the role of university general mathematics education has become undeniably important. It is necessary not only for students in science and engineering, but also for those in the humanities and social sciences, to have access to balanced mathematical education. To this end, universities must focus on developing a variety of general mathematics courses. This paper presents a detailed case study of the design and operation of a basic general mathematics course titled “Mathematics and Life Sciences,” offered to students in the humanities and social sciences at Y University in Seoul. The course is structured to enable students to learn essential basic mathematics theories that are crucial across various disciplines and to apply them to phenomena in life sciences. In doing so, students will gain a better understanding of certain interdisciplinary connections as well as enhance their thinking and problem-solving skills. Additionally, this paper analyzes the educational effects and positive feedback from students obtained through a decade of continuous improvement in the content and operation of this course. Based on this analysis, this paper proposes future directions for development and research tasks in the educational field. This research is expected to contribute to the exploration of the direction in which university general mathematics education should head.