Collection Of Plants Research Articles

Development of an Artificial Intelligence-Based Mobile Application Platform: Evaluation of Prospective Science Teachers’ Project on Creating Virtual Plant Collections in terms of Plant Blindness and Knowledge

This research aims to investigate the use of an artificial intelligence-based mobile application with plants and plant identification techniques (AImPLANT) with prospective science teachers in outdoor activities. The goals of the study are: i) to develop an AI-based mobile application from scratch based customized for education ii) to enhance knowledge about common plants iii) to increase awareness of plants, animals, and the natural environment, offering solutions to reduce plant blindness iv) to support outdoor activities to observe, identify and taxonomically determine specific characteristics of plants (endemic, economic, health, aesthetic etc.) v) guide students creating an individual virtual herbarium. The research will achieve the expected goals by coding an original “AI-Based Mobile Application” using ChatGPT and PlantNet APIs through technologies like Flutter, Firebase, Firestore, involving approximately 3200 lines of code. The mobile application includes components such as an “Plant collection (herbarium) project module”, “AI-based chatbot", "AI-based plant identification module", "AI-based student assessment module", and a "Student assistance section". The application provides guidance in creating virtual plant collections (herbariums). Additionally, aids by the “help module” and “chatbot”. Furthermore, an AI-based “self-assessment module” evaluates like a teacher based on the answers. The research question and sub problems were based on prospective science teachers' knowledge about plant identification and taxonomy, plant blindness, and opinions on studying with artificial intelligence outdoors with common plant species. The research, once again showed that artificial intelligence facilitates teaching biology, increases academic success, has positive contributions to eliminating plant blindness, and reduces teacher candidates' concerns about artificial intelligence and affects their opinions positively.

Biography and contributions of Joaquim do Espírito Santo (1901-1984) to the botany of Guinea-Bissau and São Tomé and Príncipe

A biography of Joaquim Viegas da Graça do Espírito Santo (1901–1984) is provided by integrating information from botanical collections and from his record as a civil servant. Born in São Tomé Island (São Tomé and Príncipe), he finished his studies in Portugal in 1924, spent most of his career as an agricultural technical engineer in Guinea-Bissau (1927–1956), and after retiring continued collecting, mostly in São Tomé. He collected at least 5 412 herbarium specimens, including 23 types of plant names. He remains the most prolific plant collector in Guinea-Bissau and is a somewhat neglected collector in São Tomé and Príncipe. He also produced 22 publications. Finally, these contributions are contextualised, taking into consideration that he was one of the few non-white plant collectors from the Portuguese colonial period, while being a member of a family that was pivotal in the inception of independence movements in Lusophone Africa.

Más de 100 adiciones a la flora de Sinaloa: la contribución de nuevas exploraciones y la ciencia ciudadana a la investigación botánica

Background: Sinaloa is one of the states with the lowest number of botanical collections in Mexico, but with the highest number of observations on the iNaturalist/iNaturalistMX platform. In 2021, the floristic list of Sinaloa was published, but since then new explorations and discoveries have been made. Questions: How many and which vascular plant species should be added to the list of Sinaloa? What sources of information support them? Species studied: Vascular plants. Study site and years: Sinaloa, Mexico (2021-2024). Methods: Literature, herbaria, digital repositories, iNaturalistMX and botanical explorations were reviewed. Richness analyses were performed with species added by recent explorations and compared with collection databases and iNaturalistMX. Results: 130 species were added to the flora of Sinaloa, including three families (Podocarpaceae, Simaroubaceae and Symplocaceae) and 18 genera. Podocarpaceae and Simaroubaceae were also recorded for the first time for the Sierra Madre Occidental. The main sources of information were iNaturalistMX, recent collections and literature omissions. Ninety-two of the 130 additions have at least one record in iNaturalistMX, of which 31 are unique records. The areas with the most species added coincide with regions underrepresented in collections, historically considered low in richness due to collection biases. Conclusions: The flora of Sinaloa is far from completely known and collection biases have produced misinterpretations in the patterns of its richness. The use of iNaturalistMX and recent explorations allowed us to identify new areas rich in biodiversity.

Multiple genotypes of a quarantine plant pathogen detected in New Zealand indigenous plants located in a botanical garden overseas

AbstractXylella fastidiosa is a xylem‐limited bacterial plant pathogen transmitted by insect vectors. It infects a wide range of plant species and causes devastating diseases. Botanical gardens are global repositories of plant diversity exposed to local biotic and abiotic stresses. We used molecular diagnostic tools for the detection of X. fastidiosa in a collection of New Zealand indigenous plants grown in a X. fastidiosa‐infected Californian area to determine if any species were infected with this bacterial pathogen and to help inform biosecurity responses. To this end, 130 New Zealand indigenous plant species comprising 72 genera, growing in the University of California Botanical Garden at Berkeley, California, were screened for X. fastidiosa. Multiple PCR‐based methods were used to detect the pathogen at the subspecies and sequence‐type levels directly from plant material; nine plant species tested positive by at least two PCR‐based methods. All nine infections were identified as X. fastidiosa subsp. multiplex sequence types 6 or 7 by the amplification of seven housekeeping genes. Three strains were cultured in vitro and their whole genome sequences were obtained; these strains belonged to three distinct clades within subspecies multiplex, indicating that the infections were not transmitted among these New Zealand indigenous plant species. The information gathered in this study will help to assess the risk of X. fastidiosa to New Zealand indigenous flora and the potential spillover to crops of agricultural importance. The work also shows the applied value of botanical garden collections as sentinels for international plant pathogen biosecurity risk assessment.

Mobilising Historical Botanical Data as Research

Abstract The ‘Vegetable Substances’ collection consists of 8,811 surviving botanical objects and a manuscript catalogue that contains over 12,700 handwritten descriptions of the contents of these boxes, according to the collector, Hans Sloane (1660–1752). This pre-Linnean collection forms part of the founding botanical collection of the Natural History Museum in London, and currently sits at the top of the Darwin Centre. The process of transforming this physical historical collection into something digital began over a decade ago and this focus piece reflects on this digital practice to date. It describes the different stages of creating a public-facing digital resource, and how this practice of mobilising data functions as research.

Indigenous Knowledge and the Hann Expedition: Re-Examining Scientific Collections From Colonial Expeditions

An examination of the motivations, dynamics and activities of colonial exploring expeditions can provide valuable insights into the ways in which the colonial mindset shaped the development of natural history collections in colonised lands. The Indigenous Knowledge and the Hann Expedition: Re-examining scientific collections from colonial expeditions project is working to redress these omissions in the historical record by re-contextualising the botanical specimens collected on the 1872 Northern Expedition and re-connecting them with the Aboriginal Country from which they were collected (Dalley et al. 2024). The 1872 Northern Expedition, led by William Hann, sought to establish the mineral and agricultural potential of Cape York Peninsula in northern Queensland, Australia. Alongside Hann, the expedition party included Jerry (Aboriginal guide), Dr Thomas Tate (botanist), Norman Taylor (geologist) and three other European men: Warner, Stewart and Nation. It was this expedition, with its reports of gold on the Palmer River, that triggered Queensland’s biggest goldrush and led to an influx of more than 20,000 settlers, with disastrous consequences for the Aboriginal people who occupied the area (Taylor and Huxley 2020). Between June and November, the expedition party travelled from Mount Surprise north to the Stewart River, before returning south via the coast at Cooktown and Weary Bay (Taylor and Huxley 2020). Geological specimens were collected by Taylor, and botanical specimens were collected by Tate and Taylor (Dowe and Taylor 2019). The botanical specimens collected by Tate and Taylor, now held at Royal Botanic Gardens Kew (K) and the National Herbarium of Victoria (MEL), were enumerated by John Dowe and Peter Taylor (Dowe and Taylor 2019). It has since emerged that the Queensland Herbarium (BRI) holds an additional 18 botanical specimens collected by Tate during the expedition (Council of Heads of Australasian Herbaria 2024). These specimens—along with expedition diaries and maps—will provide a stimulus for regional engagement that explores the intersection between the Hann expedition, colonial science, and the ongoing impacts of colonial violence on Indigenous peoples. The project is being led by three direct descendants of members of the expedition party: Nicole Huxley (descendant of Jerry); Peter Taylor (descendant of Norman Taylor) and Cameo Dalley (descendant of Thomas Tate), making the project uniquely—and powerfully—positioned to examine the personal, scientific and sociopolitical legacies of the expedition (Dalley 2021). It was co-designed with Balkanu Cape York Development Corporation and the three herbaria that house the expedition’s botanical collections. Through engaging in truth-telling with Aboriginal knowledge-holders on Cape York Peninsula to openly share and discuss historical truths around colonial violence and disposession—while highlighting the strength and resilience of First Peoples (ANTaR 2024)—the project team hope to correct historical omissions that have stood for more than 150 years and provide a more complete picture of the way in which colonial plant collections were made. Initial engagement is being undertaken with Aboriginal communities in Cape York will be undertaken to generate shared history-making and elevate Indigenous knowledge and memory to develop countervailing historical narratives and recontextualise the botanical specimens collected on the expedition. All engagement with Aboriginal communities will be held on-Country, i.e., on lands to which the Aboriginal peoples being consulted are connected via law, cultural practice, spiritual belief, language and/or custom (AIATSIS 2024). At the time of writing, the three descendants are continuing a series of region-specific engagements that are generating interest in—and support for—Indigenous-led knowledge-sharing and the documentation of untold stories. Based on this initial show of support, the project partners will likely seek funding for a broader Indigenous-led program of on-Country truth- and storytelling around key events that followed the 1872 Hann Expedition. Alongside the on-Country truth-telling, the project will help herbaria develop protocols for recording Indigenous Cultural Knowledge (ICK) shared by Aboriginal knowledge-holders in ways that uphold cultural safety and principles of Indigenous Data Sovereignty and Data Governance. Through two-way knowledge sharing, we hope to enrich the specimens both culturally and scientifically by: acknowledging the histories of specimens and the Traditional Owners of the lands on which they were collected; accommodating ethnobotanical enrichment of specimens by recording ICK alongside Western scientific knowledge in collection management systems, if requested by Traditional Owners; and connecting specimens to Country via enhanced georeferencing utilising Aboriginal knowledge of plants and place, and Expedition resources. acknowledging the histories of specimens and the Traditional Owners of the lands on which they were collected; accommodating ethnobotanical enrichment of specimens by recording ICK alongside Western scientific knowledge in collection management systems, if requested by Traditional Owners; and connecting specimens to Country via enhanced georeferencing utilising Aboriginal knowledge of plants and place, and Expedition resources. This symbiotic blending of botanical science and Indigenous Cultural Knowledge will 'give voice to Country' through knowledge partnerships, helping to heal the past, and to better understand and conserve the complex biodiversity of Cape York.

The anomalous Mr Hubert H.D. Griffith (1877–1958), Australian field naturalist, collector and benefactor

ABSTRACT Hubert H.D. Griffith was a significant but neglected contributor in the scientific discovery of Australia’s natural heritage. Although his interests were forged in his youth in Tasmania, his return to Adelaide as a young man heralded an energetic engagement with leading scientific societies and figures over nearly two decades at the beginning of the twentieth century. His interests were wide, spanning insects, birds, plants and photography. In particular, he supported J.M. Black in his preparation of the Flora of South Australia. Griffith’s impressive collections of beetles and plants have been consulted by taxonomists for more than a century and are now preserved in various state and national museums and herbaria in Australia. One genus (Griffithia) and 14 species of beetles and an important southern Australian seagrass, Amphibolis griffithii, are named after him in recognition of his contributions to science. A longtime and respected citizen of Adelaide, another enduring legacy of Hubert and his wife May (née Scott) is the Griffith Hospital, a private rehabilitation hospital they endowed in the 1950s, which continues to provide valued services to the South Australian community.

Integrating datasets from herbarium specimens and images to treat a Neotropical myrtle species complex.

Herbaria are the most important source of information for plant taxonomic work. Resources and technologies available today, such as digitised collections and herbarium DNA sequencing, can help accelerate taxonomic decisions in challenging plant groups. Here we employ an integrative methodology relying exclusively on herbarium specimens to investigate species boundaries in the Neotropical Myrcia neoobscura complex (Myrtaceae). We collected morphometric data from high-resolution images of herbarium sheets and analysed it using hierarchical clustering. We posteriorly tested the obtained morpho-groups with phylogenomics using the Angiosperms353 probe kit. We also gathered phenological and geographic information from specimen labels and built phenological histograms and ecological niche models to investigate ecological differences amongst taxa. Current circumscriptions of Myrcia arenaria, Myrcia neoglabra and Myrcia neoregeliana are confirmed in this study. Conversely, the four pieces of evidence together support Calyptranthes langsdorffii var. grandiflora, Marlierea regeliana var. parviflora and Marlierea warmingiana as separate from Myrcia marliereana, Myrcia neoriedeliana and Myrcia neoobscura, respectively, contrary to arrangements proposed by previous authors. Integrated analyses also support separation between Myrcia excoriata and two similar, undescribed taxa. Our data reveal the need for major changes in the systematics of the group, with recognition of 12 species. The successful delivery of our study aims was possible due to obtaining robust, high-quality data from museum specimens. We emphasise the importance of maintaining botanical collections physically and digitally available for taxonomic work and advocate their use to accelerate holistic taxonomic solutions of tropical species complexes. This is urgent, given the paucity of funds for fieldwork and unprecedented rates of habitat loss in the tropics.

Enhancing Taxonomic Skills in West Africa and Supporting the Growth of GC Herbarium (Ghana)

Training the next generation of environmental scientists is critical to the future of life on Earth as acknowledged in 2021 by the Royal Botanic Gardens, Kew (RBG, Kew) in Our Manifesto for Change. RBG, Kew is also committed to strengthening capacity in the most biodiverse tropical countries by sharing expertise and collections, to enhance the global conservation effort. Developing training programmes to enhance taxonomic capacity in Africa and meeting the demand for early career in-country taxonomists was identified as the first step to accelerate the documentation and conservation of African plant diversity. Two years ago, RBG, Kew, in collaboration with the University of Ghana and the Ghana Herbarium (GC) and with support from the Schroder Charity Trust and Mallinckrodt Foundation, developed a tailored “Plant Taxonomy Skills for Ecology and Conservation” training course. This was planned to run in Africa, South America and Asia, alternating each year. The pilot was organised in Ghana, Africa in 2022. Participants for this 5-week course were selected from over 260 applicants. Delivered in a hybrid format, the course started with 3 weeks of online lectures, pre-recorded by 15 plant scientists and curators at RBG, Kew, as well as online interactive sessions. This was then followed by 2 weeks of in-person training in Ghana, including a 1-day field trip for practical collecting tips. The training covered herbarium curation techniques as well as concepts and skills needed for taxonomic revisions such as nomenclature, taxonomy, analysis of herbarium specimens, databasing, geo-referencing, preparation of morphological descriptions, diagnosis and identification keys as well as building distribution maps and red listing. The in-country teaching team included a curator-botanist, a plant taxonomist, a science education coordinator from RBG, Kew and two professors from University of Ghana. The course ended with a written assignment in the form of a taxonomic revision. A short survey was sent out to all course participants two years after the course to gather impacts of the training on their careers and uncover the most positive and negative aspects of the course. The course provided many positive outcomes: 15 plant taxonomists from eight African countries were trained, and jointly produced a taxonomic revision of 28 species of Ipomoea L. (Convolvulaceae) from Ghana (Williams et al. 2024). Additionally, The African Taxonomists Network was initiated by the course participants with a mission of ‘Fostering the next generation of taxonomists to bridge the generational gap and revitalize the relevance of taxonomy in Africa for the future.’ This network now has over 60 members, meets regularly online, and has a large presence on social media. Furthermore, the course also resulted in the awarding of the International Association for Plant Taxonomy (IAPT) Biodiversity Challenge grant to the GC Herbarium in 2022. This grant enabled acquiring essential plant collecting equipment and herbarium materials, either by sourcing them in Ghana or from abroad. The GC herbarium, founded in 1952, contains ~85,000 collections of plant and algae specimens from Ghana and across West Africa with high historical, teaching and research value. This collection is vital to enhancing in-country taxonomic capacity. Therefore, the improvement and upgrade supported by the grant, as well as its recognition, are essential. Another rewarding impact of the course is strengthening of links between RBG, Kew and African institutions of higher education. These links have quickly developed into a larger partnership and were the first step towards a long-term collaboration. The ultimate objective of this collaboration has been to create the necessary conditions, both in infrastructure and human capacity to deliver the Flora of Ghana. This will be achieved by implementing a project to support the maintenance and growth of the GC herbarium in addition to taxonomic capacity building and teaching. The participants benefited from the course by engaging with mentors, networking and exchanging ideas, forming links with individuals with similar research interests and obtaining additional training and career progression opportunities. In the feedback survey, all participants reported that this course made them consider a career or future studies in plant taxonomy and 80% of the participants reported securing a new job or a student position in plant taxonomy two years after the course. In addition, all participants listed several positive impacts of the course on their career such as confidence in producing a taxonomic revision, gaining herbarium management skills and opportunities for new collaborations. This course has been subsequently successfully run in South America (Bolivia) and Asia (Indonesia), with expectations of similarly positive outcomes, and is already planned to return to Africa (Kenya) in 2025.

Qualitative phytochemical analysis of bioactive constituents in acetone extract of seeds of Hydnocarpus pentandra

Ethnoveterinary medicine focuses on the practical use of community or area based traditional and indigenous knowledge including the collection, preparation and administration of medicinal plants or plant parts for curing diseases of animals. Hydnocarpus pentandra is a plant endemic to South India which is traditionally known for the antileprotic activity of its seed oil. Diverse pharmacological activities of the plant extracts depend on the presence of its secondary metabolites. The present research was conducted to explore the phytochemical constituents present in the acetone solvent extract of the seeds of H. pentandra. Preliminary phytochemical screening of the extract using colorimetric reactions revealed the presence of steroids, triterpenes, diterpenes, flavonoids, alkaloids and carbohydrates. Gas chromatography-mass spectrometry (GC-MS) analysis revealed the presence of different compounds which included paromomycin, D-streptamine, carnegine, 4-propyl acridine and R-limonene. The acetone extract could be considered as a potent source of new molecules with different pharmacological actions. Keywords: Hydnocarpus pentandra, phytochemical screening, secondary metabolites

Lotus growth and development in artificial water bodies in the conditions of the Stavropol Upland

Relevance. The creation of a botanical collection of the lotus family made it possible to study the biology and adaptive features of a rare relict species of Nelumbo nucifera and its variety in the conditions of the Stavropol Upland.Methods. The main phases of development (the beginning of growth, budding, flowering) were recorded. Statistical processing was carried out by the method of variance analysis according to G.N. Zaitsev.Results. In 2010, Nelumbo nucifera seeds were purchased from the Volga River Delta and planted in a 100-liter container, which was located in a closed ground. From 2010 to 2014, the plant vegetated every year from the second decade of January to November, in November, vegetation was artificially stopped. With such agricultural techniques, the plant had no flowering and fruiting phases. In June 2014, rhizomes were planted and lotus seeds were sown in a pond made of butyl rubber film. In 2017, the first flowering was observed. During the study period, the plants did not reach natural indicators, on average, the diameter of the leaves is less by 18.5 ± 1.6 cm, the flowers are 2 ± 0.1 cm, the height is 1.0–1.5 m. The duration of flowering is 57 ± 4 days, which corresponds to the natural conditions of lotus growth from mid-July to early September. During the years of research, 495 seeds were obtained. Under the influence of abiotic factors, the nut-bearing lotus lives up to 8 years in the botanical garden. Nelumbo nucifera Betsy grows intensively, flowering occurred in the third year after sowing, leaf diameter 57 ± 3 cm, height above the water surface 78 ± 7 cm, flowering duration 73 ± 7 days, which is longer than that of the nut-bearing lotus, full-fledged seeds are formed.

Ex-Situ conservation practices for some endangered plants in Ankara, Türkiye

Within the scope of this study, activities were carried out to ensure ex-situ conservation of 12 taxa, distributed Ayaş, Beypazarı and Nallıhan districts in Ankara province, in the collection garden established by the National Botanical Garden of Türkiye and to raise awareness. With this study, which includes a part of the project supported by the General Directorate of Agricultural Research and Policies with the number TAGEM/BBAD/B/20/A7/P9/1820, the habitat characteristics of 12 taxa namely Achillea ketenoglui, Aethionema dumanii, Aethionema turcica, Astragalus densifolius subsp. ayashensis, Astragalus beypazaricus, Astragalus yildirimlii, Asyneuma linifolium subsp. nallihanicum, Campanula damboldtiana, Cytisus acutangulus, Salsola grandis, Salvia aytachii and Verbascum gypsicola, were recorded and analyzes were carried out using Geographic Information Systems. In line with the analyses, the most suitable place to establish an Endemic Plants Collection Garden within the borders of the National Botanical Garden of Türkiye was selected. Among the taxa studied, 7 propagated species were adapted and protected in the collection garden. Thus, an important example study has been presented for the conservation of endangered taxa, which is one of the missions of botanical gardens.

Mobilizing International Australian Collections Locally for Global Benefit

From the earliest days of Australian colonization and exploration, botanical collections have been sent to herbaria overseas for western scientific study. Where there is a duplicate set, collections have been repatriated back to Australia with time, but many specimens remain housed overseas. These specimens document the floral diversity of Australian ecoregions that have now been altered, and of species that have not yet been described and may now have been lost to our ecosystems. Many such collections have type specimens, and others have historical exploration and botanical significance, many of which are not readily available for taxonomic and cultural research. One such collection, the Cambridge University Herbarium (CGE), is the fourth largest herbarium in the United Kingdom (Gardiner 2018) and contains more than 13,700 specimens of Australian origin. A collaborative project between the Western Australian Herbarium (PERTH) and CGE, supported by The Winston Churchill Memorial Trust, Australia, has enabled Australian collections to be located, updated, digitized, mobilized, and shared—decolonization and repatriation in digital form. With only five weeks to undertake collection-based activities at CGE, the effort focused on the Australian vascular plant collections. These were systematically located and retrieved from the CGE world collections of flowering plants and ferns (conveniently geographically arranged), and the John Lindley and Charles Fox Bunbury collections, which were only partially incorporated into the world collecitons. Curation was limited to significant repairs and incorporation of loose specimens into the collections. Annotated type specimens were placed within separate folders for easy future identification and curation. Barcodes were adhered to each sheet, and basic metadata was gathered prior to imaging (taxonomic name on the sheet, storage location, type status). Sheets were imaged using two Nikon D850 45.7MP digital single-lens reflex cameras in a lightboxes; image files were converted to 16-bit TIFF for archival storage, full-sized JPGs, and 1MB JPGs for online delivery. With approximately half of the specimens transcribed in September, 2024, preliminary findings include the following: The majority of the Australian collections in CGE are from Western Australia, Tasmania, New South Wales, and South Australia with significantly fewer collections from other Australian states and territories. The majority of specimens were collected in the 1800s and early 1900s, with dominant collectors being James Drummond (collecting in Western Australia), Ronald Gunn (collecting in Tasmania), William Philip Hiern, John Lhotsky, Joseph Jackson Lister, Sir Thomas Livingstone Mitchell, Georgiana Molloy, Fredrick Stimpson Salisbury, and William John Swainson. Prominent individual personal herbarium collections represented included those of Charles Morgan Lemann (1806–1852), John Lindley (1799–1865), John Stevens Henslow (1796–1861) and Charles Edward Moss (1870–1930). Most specimens have little locality information (e.g, "New Holland", "Van Diemen's Land", "Swan River"). More than 475 type specimens have been identified. More than 145 vascular plant families, 875 genera, and ca. 4500 (many non-current) species are housed within the collections (representing approximately 15% of Australian vascular plants). The majority of the Australian collections in CGE are from Western Australia, Tasmania, New South Wales, and South Australia with significantly fewer collections from other Australian states and territories. The majority of specimens were collected in the 1800s and early 1900s, with dominant collectors being James Drummond (collecting in Western Australia), Ronald Gunn (collecting in Tasmania), William Philip Hiern, John Lhotsky, Joseph Jackson Lister, Sir Thomas Livingstone Mitchell, Georgiana Molloy, Fredrick Stimpson Salisbury, and William John Swainson. Prominent individual personal herbarium collections represented included those of Charles Morgan Lemann (1806–1852), John Lindley (1799–1865), John Stevens Henslow (1796–1861) and Charles Edward Moss (1870–1930). Most specimens have little locality information (e.g, "New Holland", "Van Diemen's Land", "Swan River"). More than 475 type specimens have been identified. More than 145 vascular plant families, 875 genera, and ca. 4500 (many non-current) species are housed within the collections (representing approximately 15% of Australian vascular plants). Full transcription of label data (collector, collection date, collection number, locality information, and other collection information) and taxonomic curation is ongoing. Data and high resolution images will be made available soon to the scientific community, historians, and the public for data curation and annotation through the Atlas of Living Australia and other digital platforms. Access to these specimens will be invaluable for botanical research in this age of accelerated biodiversity loss, and as travel to, or shipping of, physical specimens is increasingly fraught and costly.

Changes in the Phylogenetic Structure of Alpine Grassland Plant Communities on the Qinghai-Tibetan Plateau with Long-Term Nitrogen Deposition.

Nitrogen (N) deposition rates have notably increased around the world, especially in high-altitude regions like the Qinghai-Tibetan Plateau (QTP). We conducted a six-year comprehensive experiment to simulate nitrogen deposition in an alpine grassland area near Qinghai Lake. Four levels of nitrogen depositions, i.e., 0 (CK), 8 kg N ha-1year-1 (N1), 40 kg N ha-1year-1 (N2), and 72 kg N ha-1year-1 (N3), with three replicates for each N treatment, were tested annually in early May and early July, with the meticulous collection of plant and soil samples during the peak growth period from 15 July to 15 August. We used the null model to evaluate the impact of environmental filtration and interspecific competition on the dynamics of the plant community was assessed based on the level of discrete species affinities within the plant community by constructing a phylogenetic tree. The results showed that the environmental filter was the predominant driver for the change of community's genealogical fabric. The N2 and N3 treatments increased the influence of soil factors on the change of plant community structure. Climatic factors played a crucial role on the change of plant community in the CK grassland area, while soil factors were dominant in the N1- and N3-treated grasslands.

Impact of season on phytochemistry with special reference to β-Asarone content, extract yield and Pharmacognostic parameter in rhizome of Acorus calamus L.

Demand of medicinal plants is growing exponentially due to its extensive use in nutraceutical, cosmeceutical and pharmaceutical industries. The major problem is the variation in quality and quantity of phytoconstituents with the collection of medicinal plants in each batch. Though, the quality and quantity of phytoconstituents in plants are influenced by various factors, chief among them is seasonal variation. Rhizome of Acorus calamus L. is used in treatment of neurological disorder specially in children. The Ayurvedic system of medicine believes blends of constituent imparts therapeutic activities. The main chemical constituent responsible for cognition enhancement is Asarone. An attempt has been taken to record change in Pharmacognostic parameter with season and evaluate the best collection time of rhizome of Acaorus calamus L. with special reference to extractive value and asarone content.

Ethnobotanical study of wild edible plants in the mountainous regions of Semnan Province, Iran

In mountainous areas, wild edible plants are an important part of the local diet. Climate change and anthropogenic activities have profound effects on wild edible plants in these areas. Ethnobotanical studies are important for understanding the use patterns and harvest impacts on these plants. In this regard, the Shahrood region, with its diverse historical/ethnic background, is an appropriate starting point to investigate exploitation patterns and impacts of harvest of wild edible plants. During 2021–2022, ethnobotanical surveys were conducted in 12 villages in the region using semi-structured interviews and participatory observations. Data collection included assessing wild edible plant species diversity, their habitats, collection time and quantities, plant parts used and methods of consumption and preparation. The data were analyzed using use report indicators, relative frequency of citations, and use value (UV). A total of 1086 use reports were documented from a total of 44 interviews. A total of 67 wild species (66 plants and 1 fungus) belonging to 54 genera and 24 families were used as edible in the study area. Rosaceae (9 species), Apiaceae (8), Lamiaceae (8), Asteraceae (7), and Amaryllidaceae (5) were the families with the most reported species. Allium (5 species) was the most diverse genus in terms of species diversity. Herbs were the most commonly used life form (79.1%), followed by shrubs (13.4%) and trees (6%). The most consumed plant parts were young leaves (25%) and young aerial parts (21.4%). There were 13 use categories identified. The majority of edible plants were collected in April–May, mainly from areas nearby villages (37.7%) and rangelands (33.7%). The most important edible plants in the study area were Allium iranicum (Wendelbo) Wendelbo (UV = 1.7), Mentha longifolia (L.) Huds. (1.5), Allium umbilicatum Boiss. (1.47), Tragopogon graminifolius DC. (1.38), Lepidium draba L. (1.27), Urtica dioica L. (1.18), Falcaria vulgaris Bernh. (1.13), Malva neglecta Wallr. (1.11) and Eremurus sp. (1.09). Our results showed diverse and valuable knowledge for wild edible plants in this region, which should be considered in the conservation and management plans in the region.

Towards a conceptual design framework for bee botanic gardens: integrating perceptions on urban biodiversity into landscape design processes

Botanic gardens, valued for their live plant collections, have evolved into scientific institutions with roles in conservation, education, research, and public recreation. This study repositions botanic gardens as essential urban habitats enhancing biodiversity, focusing particularly on their support for both European honeybees and Western Australian native bees. This research addresses the intersection of urbanisation and bee conservation by developing a conceptual design for a bee-friendly botanic garden in Yanchep, Western Australia. It employs semi-structured interviews and research through design (RtD) methods to collaborate with local beekeepers, landscape architects, and ecologists. A systematic design framework for a bee-friendly botanic garden in Yanchep, Western Australia was proposed, encompassing the entire design process, from scope to detailed considerations, and aiming to be applicable across other parts of Western Australia and Australia. The research highlights three key findings: the necessity of interdisciplinary collaboration, the importance of defining the garden type and functions early in the design process, and the need for diverse plant selection and nesting options to support native bees.

The Biology of Invasive Native Plant as an Ex-situ Collection: A Case Study of Epipremnum pinnatum (L.) Engl. (Araceae) in Bogor Botanical Garden, Indonesia

The Bogor Botanical Garden (BBG) is a pivotal center for plant research and conservation, boasting a diverse collection of over 12,000 individual plants spanning various species. However, among the plant collections at BBG, some of them have the potential to be invasive. Therefore, this study aimed to uncover the plant collections at BBG with invasive potential and analyze the distribution of one of the most dominant collections, Epipremnum pinnatum (L.) Engl. The method employed in this research involved a literature review to identify which plant collections have invasive potential and a vegetation analysis on the distribution of E. pinnatum within BBG. Additionally, ANOVA analysis and regression models were employed to explore the relationship between environmental factors and E. pinnatum abundance. Results revealed that BBG hosted 78 potentially invasive plant species, with E. pinnatum comprising 28 individuals. Spatial analysis indicates a clustered distribution of E. pinnatum, influenced by seed dispersal mechanisms, environmental factors, and biotic interactions. Correlation analysis links soil pH, soil moisture, and canopy cover to E. pinnatum distribution. Canopy cover demonstrates a significant positive correlation with E. pinnatum abundance, suggesting its importance in providing favorable microclimates for growth. Regression analysis further supports canopy cover as a predictor of E. pinnatum presence. However, while a strong statistical association was observed, causal relationships require further investigation. This study underscores the complexity of ecological dynamics in BBG and emphasizes the need for comprehensive research to define underlying mechanisms driving plant distributions and interactions.

The 2030 Declaration on Scientific Plant and Fungal Collecting

Societal Impact StatementBiological samples and their associated information are an essential resource used by scientists, governments, policymakers, practitioners and communities to ensure that biodiversity can be appropriately protected and sustainably used. Yet, considering the enormous task of documenting the vast numbers of as‐yet‐unknown plant and fungal species, greater international coordination for biological collecting and recording is necessary, built on equitable collecting practices and standards. Here, we propose five commitments to accelerate and enhance scientific knowledge of plant and fungal diversity, while increasing collaboration, benefit sharing and efficiency.SummaryAlmost all life depends on plants and fungi, making knowledge of their diversity and distribution—primarily derived from biological collections—fundamental to national and international conservation, restoration and sustainable use commitments. However, it is estimated that some 15% of all plant species and over 90% of all fungal species have not yet been scientifically described, hampering our ability to assess and demonstrate the impact of efforts to halt biodiversity loss. In addition, organisations and researchers around the world lack a concerted strategy for increasing complementarity and avoiding overlap in botanical and mycological research, particularly in relation to the collection of specimens. We here present the 2030 Declaration on Scientific Plant and Fungal Collecting, summarising a commitment towards such a necessary strategy. Its components were identified from discussions during and after a series of four workshops and plenary discussions at the 2023 State of the World's Plants and Fungi symposium convened by the Royal Botanic Gardens, Kew, and were then consolidated into the present form by the authors. The Declaration was subsequently opened up for endorsement by signatories. Collectively, we agree on a set of five commitments for cataloguing the world's flora and funga, designed to maximise efficiency, facilitate knowledge exchange and promote equitable collaborations: (1) use evidence‐based collection strategies; (2) strengthen local capacity; (3) collaborate across taxa and disciplines; (4) collect for the future; and (5) share the benefits. This Declaration is a first step towards increased global and regional coordination of scientific collecting efforts.

Study of germination of collection samples of wild flora of Astana botanical garden

The present study is devoted to the main tasks of seed bank laboratories, among which the formation, study and conservation of seed collections of both natural and introduced flora of Central and Northern Kazakhstan are emphasized. This work emphasizes the importance of maintaining optimal conditions for seed collection in these regions, which is a key aspect for successful seed management, conservation and restoration of plant communities. Expedition trips, conducted annually between July and September, are synchronized with the growing seasons of the plants and take into account weather conditions that directly affect the efficiency of seed collection.During such visits, 56 forms of wild flora were collected, mainly on the territory of the Kokchetavskaya Upland, Central-Kazakhstan shallow meadow and Akmola region, including Shortanda district, as well as in the unique ecosystems of SNPP “Burabai” and the vicinity of Makinki village. The collected genetic material plays a key role in biodiversity conservation and can be used for further scientific research as well as for practical needs related to ecosystem restoration.As part of this work, a laboratory study was also conducted to investigate the viability of the selected seeds. The results of the study will help to further understand the characteristics and factors that determine the viability of seeds of different forms, which is crucial for their subsequent use in botanical collections. This study emphasizes the relevance of the selected topic, as well as the importance of conservation and management of seed resources in conditions of ecological vulnerability, which is of significant importance for sustainable environmental protection and conservation of plant diversity in Central and Northern Kazakhstan.