ABSTRACT The collection and transportation activities engaged in waste management (WM) are known to have significant environmental impacts. This study assessed the current environmental situation regarding the use of diesel fuel in hazardous waste (HW) collection and transportation using a LCA methodology. The study utilized the actual transportation data of the HW collection and transportation activities of a WM company, IZAYDAS, for the year 2021. The functional unit (FU) chosen for analysis was the “environmental impact per annual total fuel consumption.” In addition, the study aimed to understand potential changes in environmental impact if electricity were used as a fuel alternative. The findings revealed that in the current scenario with diesel fuel, the Abiotic Depletion Potential (Fossil Fuel) (ADP Fossil) effect category had a dominant effect (3.92E + 06 MJ). The total Global Warming Potential (GWP100a) effect for HW transportation was determined to be 5.28E + 05 kg CO2 eq in 2021. When electricity was considered a fuel alternative, a decrease was observed in the GWP100a and ADP impact categories. At the same time, Human Toxicity (HTP), Acidification (AP), and Eutrophication (EP) showed an increase due to fossil fuel-based energy production. Sensitivity analysis indicated that all effects are lower in countries that depend on clean energy sources for electricity production.