School-enterprise collaborative training model is a system of cultivating talents through school-enterprise cooperation and combining practice. This paper explores the practical model of university collaborative innovation based on symbiosis theory, aiming at examining and analyzing the complex synergistic relationship between each inner symbiotic unit, revealing the inner practical evolution logic, and providing useful reference and inspiration for the improvement of university collaborative innovation performance. The symbiotic model of collaborative innovation between universities and enterprises interacting with each other is constructed, and innovation is carried out with market demand as the link. Based on this, e-commerce majors in colleges and universities should integrate enterprise elements, form multilateral communication mechanisms and promote the stability of symbiotic relationships. In order to verify that the symbiosis theory can innovate the school-enterprise collaborative training model, a school-enterprise cooperation internship training platform was built. 19.1% of students felt very dissatisfied and 18.9% felt very satisfied with it before the internship. The students who were very dissatisfied after the internship decreased from 19.1% to 2.1% before the internship, and 53.1% of the students completely achieved the goal, which indicates that improving the symbiotic integrated governance system of university collaborative innovation can improve the ability of university collaborative innovation comprehensively.