Colgate Plax Research Articles

Which Whitening Mouthwash With Different Ingredients Is More Effective on Color and Bond Strength of Enamel?

To examine the effects of six whitening mouthwashes on tooth color and immediate bond strength to the enamel. Human incisors were divided into seven groups (n = 10) according to mouthwashes (R.O.C.S Black Edition White, Splat White Plus, Colgate Plax White Charcoal, Signal White Now, Listerine Advanced White, Colgate Optic White, and distilled water). After the initial color measurements, the teeth were exposed to mouthwash for 4 weeks. Then, the color measurements were repeated. Then, cylindrical composite resin blocks were immediately applied to the enamel surfaces and subjected to shear bond strength tests. Data were analyzed using Kruskal-Wallis and Bonferroni tests (α = 0.05). Δ𝑏, Δ𝐿, and ΔE00 values did not present significant differences among the groups. Significant differences among the groups were determined for Δ𝑎 and ΔWID values (p < 0.05). R.O.C.S Black Edition White and Splat White Plus produced clinically acceptable color changes. Signal White Now, Splat White Plus, and Listerine Advanced White created acceptable whiteness changes. The mouthwashes did not statistically affect the bond strength compared to the distilled water (p > 0.05). Whitening mouthwash containing blue covarine revealed more acceptable color and whitening changes. Mouthwash containing charcoal led to the lowest enamel bond strength values. The content of whitening mouthwashes affected the degree of tooth whitening and shear bond strength to enamel.

Corrosion Behavior of Nickel-Titanium Arch Wires Following the Use of Different Mouthwashes: An In Vivo Study.

The aim of this double-blind in vivo study was to compare the extent of corrosion on the surface of nickel-titanium (NiTi) wires in various mouthwashes. A total of 80 patients who received orthodontic treatment with as-received 0.016x0.022 inch NiTi wires were included in the study, and they were split into 4 groups. The first group used 0.05% of (225ppm F-) sodium fluoride (NaF) (Colgate Plax®) containing mouthwash, 21.6% alcohol (Listerine Cool Mint®) containing mouthwash, and 0.2% clorhexidine (CHX) (Klorhex®) containing mouthwash and the control group used drinking water with melt menthol as mouthwash. After 30 days of using mouthwash, the surfaces of NiTi wires were examined with atomic force microscopy (AFM), and surface roughness values were calculated. Mouthwashes containing fluoride, essential oils, and CHX created higher surface roughness on NiTi wires than the control group. The floride-containing mouthwash group showed less corrosion than the CHX group, whereas there was no difference between the essential oil group. AFM images show supportive data with the results of the clinical study. The results were assessed using a 95% confidence interval and a significance level p<0.05. CHX, essential oil, and floride-containing mouthwashes cause corrosion of NiTi wires. Floride-containing mouthwash can be preferred over CHX mouthwash due to its lesser corrosion effect.

Evaluation of the Surface Roughness and Solubility of Newer Self-blending Composite Resin following Home-bleaching and Brushing-mouth Rinsing Cycle

Objective(s): To evaluate the effect of tooth brushing and mouth rinsing after bleaching with 16% carbamide peroxide (16% CP) on the surface roughness and solubility of newer self-blending resin composite. Materials and Methods: About 70 specimens were fabricated with Estelite sigma-quick composite resin and subjected to bleaching using custom-tray with 16%CP gel for 6hours except 10 samples to serve as control group and divided into seven groups (n=10) as follows: group 1: no bleaching (control), group 2: 16% CP, group 3: brushing, group 4: brushing+Listerine mouth rinse, group 5: brushing +Colgate Plaxmouth rinse, group 6: Listerine, group 7: Colgate Plax. Electric tooth brushing was done using toothpaste slurry for 3min, immersed in mouth-rinse of respective groups for 1min and placed in artificial saliva for 24hrs. This was done for a total period of 21 days followed by surface roughness and solubility testing. Data were statistically analyzed using one-way ANOVA and post hoc Tuckey tests (p&lt;0.05). Results: Group 4 (Brushing + Listerine) showed greater surface roughness and solubility followed by group 6 (Listerine). No significant difference was found between group1 with group3 and group 5. Conclusion (s): Along with brushing, Listerine mouth rinse has a determining effect on surface roughness and solubility of newer self-blending composite resin following bleaching with 16% Carbamide peroxide.

Influence of Five Different Commercially Available Mouthwashes on the Growth of Candida albicans Adhered to Customized Prefabricated Heat-Cured Denture Base Acrylic Resin Sheets: An In Vitro Study.

Background The purpose of this in vitro investigation was to evaluate the impact of five distinct commercial mouthwashes on the development of Candida albicans that had been adhered to heat-cured acrylic resin sheets. Methods This in vitro investigation was carried out at the MES Medical College's Microbiology Department in Perinthalmanna, Kerala, India. A total of 72 heat-cured acrylic resin sheets, size 10 × 10 × 2 mm, were fabricated. After disinfection, all 72 acrylic sheets were placed in a flask containing a suspension of the standard strain of Candida species (American Type Culture Collection) and incubated at 37ºC for 24 hours. Then, the acrylic sheets were randomly divided into six groups, with each group containing 12 acrylic sheets. Group 1 was the control group to which no mouthwash was added. In group 2, Colgate Plax was added. In group 3, Hiora Himalaya was added. In group 4, Oral B was added. In group 5, Listerine was added. In group 6, Pepsodent was added. Colony-forming units (CFUs) were assessed using a colony counter every six, 24, 48, and 120 hours. After obtaining the pH and CFU of all 72 specimens, software known as the Statistical Package for Social Sciences (SPSS) (IBM Corp., Armonk, NY) was used to analyze the data. Results Candida albicans adhered to heat-cured denture base acrylic resin sheets differed significantly in response to commercially available mouthwashes (Oral B, Colgate Plax, and Pepsodent) and non-commercial mouthwashes (Hiora Himalaya and Listerine) that contained cetylpyridinium chloride. Conclusions Compared to other mouthwashes that do not contain cetylpyridinium chloride (Listerine and Hiora Himalaya), mouthwashes with cetylpyridinium chloride as the active ingredient (Oral B, Pepsodent, and Colgate Plax) have shown good antifungal properties against the adhering Candida albicans on denture base resin.

Effect of mouthwash on color change and solubility of composite resins

Aims: This study aimed to evaluate the effects of currently available mouthwashes on color changes and solubilities of composite resins by comparing them. Methods: Three different types of composite resins, namely nanofill, nanohybrid, and microhybrid resins (Filtek Ultimate, Estelite Sigma Quick, and Filtek Z250, respectively), were used in thisstudy. A total of 120 samples, with 40 foreach composite material, were prepared. The composite resins were placed in plastic molds of 10 mm × 2 mm with round disc shapes and polymerized with an LED light device. After finishing and polishing them with a polishing disc, the prepared samples were stored in distilled water. Initial color measurements were performed using the VITA Easyshade V device (VITA, Germany) for color stability evaluations. For solubility measurements, the dry weights of the samples were measured and their diameters and thicknesses were recorded with a caliper. Samples from each group were then immersed in three different mouthwashes (Kloroben, Listerine Advanced White, and Colgate Plax) twice a day for 2 minutes for 14 days. After 14 days, color and weight measurements were repeated for all samples. Kruskal-Wallis and Mann-Whitney U tests were used in the statistical analysis of the data. Results: Significant color changes were detected in all samples as a result of color measurements (p&lt;0.05). Significant differences were observed for all solution groups in terms of color change, but the lowest amount of color change was observed with the Colgate Plax solution. Comparisons of composite groups revealed that Filtek Ultimate was the composite with the lowest amount of color change. Positive solubility values were recorded for all samples as a result of solubility measurements. Although no significant difference was observed among composite groups in terms of solubility, higher solubility levels were detected with the Kloroben, Listerine Advanced White, and Colgate Plax solutionscompared to the control group. Conclusion: In thisstudy, in all evaluated composite groups, acceptable levels of color change and solubility were detected, with values varying depending on the composite material after immersion in the mouthwashes.

Comparative evalutation of effect of different mouthwashes on color stability of different composites- an vitro study.

Abstract-&#x0D; Background- Color is the important property for the restorative materials to have long-term durability in the oral cavity. Color is important in aesthetics, characterizing and personalizing a smile.&#x0D; Aim- The aim of this study was to evaluate the effects of five commercially available mouthrinses (Listerine, Colgate plax, Clohex Plus, Orahex Plus and Senquel-AD) on color stability of three different resin-based composite restorative materials.&#x0D; Material and Methods - A custom made stainless steel split mold was used for fabrication of forty-eight samples of each composite resin material (Durafill VS, Z100, Filtek Z350 XT). The samples were immersed in distilled water after curing for 24 hours. Baseline measurements for Color stability were taken. After that, each group was immersed in 20 ml of assigned mouthrinse solutions and incubated at 37° C for 24 hours. The samples were resubjected to Color stability tests. The change in color difference was calculated.&#x0D; Statistical analysis-&#x0D; Result- The results revealed that all mouthrinses tested changed the color of the composite resins.&#x0D; Conclusions- It may be concluded that the effect was both mouthrinse and material dependent.

Assessment of the Ability of Desensitizing and Conventional Mouth Rinses to Promote Dentin Tubule Occlusion.

This study aimed to evaluate the effect of desensitizing and conventional mouth rinses on dentin tubule occlusion. Dentin hypersensitivity was simulated by EDTA application for five minutes. The specimens were randomly allocated into the following groups: desensitizing mouth rinses (Colgate Sensitive, Elmex Sensitive Professional, Listerine Advanced Defense Sensitive, Sensodyne Cool Mint); conventional mouth rinses (Colgate Plax, Elmex Caries Protection, Listerine Anticaries, Sensodyne Pronamel); a negative control (C-: distilled water); and Clinpro XT Varnish was the positive control (C+). Subsequently, the specimens were submitted to an erosive or abrasive challenge (performed separately) and to an erosive/abrasive cycling for five days (n=10 for each challenge). After treatment, challenges, and cycling, the specimens were analyzed in an environmental scanning electron microscope to verify the number of open dentin tubules (ODTs), counted by using Image J software (National Institutes of Health, Bethesda, MD, USA). Data were analyzed by the Kruskal-Wallis, Friedman and Dunn tests, with Bonferroni correction (α=0.05). Groups did not differ at baseline (p>0.05). At the post-treatment, erosion and abrasion stages, C+ was the only group that showed a reduction in ODTs compared to C-(p<0.05). In the other groups, numbers did not differ significantly from C- (p>0.05). After cycling, none of the groups exhibited significant reduction in ODTs other than C- (p>0.05); however, C+, Listerine Anticaries, and Colgate Plax had a lower number of ODTs than Listerine Sensitive and Sensodyne Pronamel. No mouth rinse was able to promote significant occlusion of the dentin tubules after treatment and the challenges. C+ was the only product that effectively promoted tubular occlusion, but this effect did not withstand several erosive and abrasive challenges.

ANTİ-HALİTOSİS GARGARALARININ REZİN BAZLI RESTORATİF DENTAL MATERYALLERİN YÜZEY ÖZELLİKLERİ ÜZERİNE ETKİSİ

Objectives: This in-vitro study aimed to investigate the effect of five different types of mouth rinses used for halitosis on color stability and surface roughness of two types of restorative materials. Materials and Methods: In this study, a total of 120 disc-shaped samples (10x2mm) were prepared with nano-hybrid resin composite (Filtek Z550) and giomer (Beautifill II) materials. Randomly selected samples were divided into six groups as five different mouth rinses (Listerine Fresh Burst, Listerine Total Care, Colgate Plax, Oderol, Halitosil) and a control group (distilled water). Initial color values were measured by spectrophotometer (Easyshade Compact) according to the CIELAB system, and roughness values (Ra) were measured by contact profilometer (Surtronic 25). All specimens were incubated in mouth rinses at 37° C for 12 hours and measurements were performed in the same procedure. Data were analyzed by using Kruskal-Wallis H and Bonferroni Post Hoc tests with the SPSS 24.0 program at a significance level of 0.05. Results: The results of this study showed that there was not a statistically significant increase in surface roughness values. There was a significant change in CIELAB values in all of the Beautifil II materials after the immersion in mouth rinses (P&amp;lt;0.05). There was a statistically significant difference between the color change values of Beautifil II and Filtek Z550 materials kept in the same mouth rinses (P&amp;lt;0.05). Beautifil II was exhibited color change with values above the clinically acceptable limit (ΔE&amp;gt;3.3). Conclusions: Color changes occurred in both of the restorative materials kept in different mouth rinses.

Surface Microhardness Of Microhybrid And Nanocomposite After Storage In Mouth Washes.

Dental composites are aesthetic direct restorative material. However, the effect of mouthwashes on the durability of the material is controversial. This study evaluated and compared the influence of mouthwash composition on the surface hardness of nanofilled (Z350XT) and microhybrid (P60) resin composites. Comparative in-vitro study was conducted over 6 months at Multan Medical & Dental College. Sixty-four disc-shape specimens of each {nanofilled (Z350XT) and microhybrid (P60)} resin composite were prepared and stored in distilled water at 37°C for 24 hours. The baseline microhardness reading (To) was recorded by Vickers micro-hardness tester. Samples were then randomly divided into four groups (n=16) and stored in Listerine Cool Mint, Colgate Plax, Clinica and distilled water (control). The hardness test was repeated after 12 hours and 24 hours of storage. Nanocomposite (Z350XT) had statistically (p<0.01) higher surface hardness. A significant reduction (p≤0.05) in microhardness was observed after immersion of samples in mouthwashes. The reduction in surface hardness was dependent on the immersion time and composition of mouthwashes. Listerine Cool Mint (alcohol-based mouthwash) had greatest degradation effect. Mouth rinses negatively impacted the surface microhardness of the tested resin-based materials. Alcohol-based mouthwashes had greater potential for reducing microhardness. Microhybrid composite appears to be a more suitable material for restoring teeth in patients accustomed to using regular mouthwashes.

Evaluation of Antimicrobial Efficacy of Three Commercially Available Herbal Mouthwashes - An In Vitro Comparative Study

Background: Today several mouthwashes are flooding the market including various herbal products. It is important to know their antimicrobial efficacy before the clinical use. Hence in the current investigation, we compared the antimicrobial efficacy of three commercially available herbal mouthwashes against four periopathogens. Methodology: In this in vitro study, comparative evaluation of antimicrobial efficacy of three commercially available herbal mouthwashes i.e Oramint herbal mouthwash, Hiora mouthwash and Colgate plax fresh tea mouthwash were tested against four different microbial strains namely Streptococcus mutans, Porphyromonas gingivalis, Candida albicans and Aggregatibacter actinomycetemcomitans. Determination of microbial sensitivity was done using ditch plate diffusion method and the zone of inhibition was measured. Results: The tested herbal mouthwashes demonstrated varying degree of antimicrobial activity against the selected pathogens. The zone of inhibition was concentration dependent for all the three mouthwashes and maximum effectiveness was observed at a concentration of 75µL/mL. Among the three mouthwashes, Oramint mouthwash showed maximum efficacy against all the pathogens except Candida albicans. Hiora and Colgate plax mouthwashes showed comparable antifungal activity and were highly effective in inhibiting Candida albicans. On intergroup comparison, the difference in the mean zones of inhibition of all the tested herbal mouthwashes was found to be statistically significant. Conclusion: Oramint mouthwash outshined the other two mouthwashes and showed maximum antimicrobial activity against all the examined microbes except Candida albicans. Hiora mouthwash and Colgate plax fresh tea extract mouthwash demonstrated good antifungal activity against Candida albicans. However, future clinical studies are needed to confirm the obtained results.

Utilização de dentifrícios e colutórios durante o tratamento ortodôntico na conduta clínica dos ortodontistas

ABSTRACT Objective: To evaluate the knowledge of orthodontic professionals regarding the prescription of dentifrices and antiseptics during orthodontic treatment. Methods: An observational and descriptive exploratory study of national scope was conducted with 440 Brazilian orthodontists, based on previous sample calculations, to evaluate the clinical practice of orthodontists in prescribing dentifrices and antiseptics. Self-managed electronic questionnaires were sent to orthodontic professionals. The data were analyzed by means of absolute and relative frequency distribution tables. Results: It was verified that the most indicated mouthwash (39.8%) was Colgate Periogard® and 421 of the participants (95.7%) know its active ingredient (chlorhexidine digluconate). Besides Colgate Periogard® antiseptic, the participants were also questioned about which active ingredient present on the following antiseptics, whose assertive answers were in the following order of %, Cepacol® (Sanofi-Aventis®) with 60.7%, Colgate Plax® (Colgate®) with 54.5% and Listerine® (Jhonson&amp;Jhonson®) with 51.6%. As for the chlorhexidine therapeutic regime, 63.9% have stated assertively its use, being this one of 2 times a day during 7 to 10 days. The most indicated tooth was Colgate Total 12®, and 60.18% of these individuals correctly indicated its active compound. Conclusion: According to the methodology employed, it could be concluded that orthodontic professionals know and prescribe methods of chemical control to patients during orthodontic treatment and most identify the active chemical compounds present in antiseptic and tooth formulas.

Effect of whitening mouth rinses on the chemical and physical properties of a nanofilled composite

Aim: This study analyzed the effect of whitening mouth rinses on water sorption (WS), solubility (SL), color change, and surface roughness of a nanofilled composite. Whitening perceptibility and acceptability (WID) were also studied. Methods: Forty specimens of Filtek Z350XT, shade EA2 were produced and randomly distributed (n=8) to AS – artificial saliva (control); LWE – Listerine Whitening Extreme; CLW – Colgate Luminous White; LCM – Listerine Cool Mint; and CP – Colgate Plax. They were immersed in the mouth rinses 2x/day, for one minute, during 28 days. The color was assessed using an Easyshade spectrophotometer (CIE-L*a*b* system). Surface roughness (Ra-μm) was measured with three parallel measures, using an RP-200 roughness meter. The WS and SL (μg/mm-3) were analyzed based on the ISO 4049 recommendations. The data were analyzed using one- and two-way ANOVA/Tukey tests (α=0.05). Results: Surface roughness significantly increased after immersion in AS and LCM, with no significant differences between the groups either before or after immersion. The ΔE* was not significantly different between the groups. All substances produced a ΔWID higher than the 50%:50% perceptibility and acceptability thresholds. The WS and SL were not significantly affected by the mouth rinses. Conclusion: Whitening mouth rinses did not affect WS, SL, surface roughness, and color stability of a nanofilled composite, regardless of the presence of ethanol in the composition.

Microhardness and surface roughness of Biodentine exposed to mouthwashes.

Aim:This study evaluates Vickers microhardness and surface roughness in Biodentine cement (M1) and glass-ionomer cement Fuji IX (M2), both immersed in mouthwash.Materials and Methods:Fifty-four samples were randomly distributed in distilled water (S1), Listerine Cool Mint (S2), and Colgate Plax (S3). Each sample was put in a flask with mouthwash for 2 min, under vibration, twice a day for 21 days. Microhardness and surface roughness were assessed at 48 h (T0), 7 days (T1), 14 days (T2), and 21 days (T3).Results:For roughness: time (T), solution (S) and material (M), TxM, and SxM and for microhardness: M, TxS, TxM, and SxM were statistically significant. T3, M1, M1T3, and M1S1 presented the highest surface roughness. M2, M1T0, M1T1, M1T2, M1S1, and M1S2 presented higher microhardness.Conclusion:Biodentine showed higher surface roughness for T1, T2, and T3 and higher microhardness for T0, T1, and T2 against Fuji IX. Biodentine presented higher microhardness independently of solution.

Color stability of orthodontic esthetic archwires- A comparative in vitro study

Objective: To study and evaluate effect of various food products on color stability of orthodontic esthetic archwires at different time period. Materials and Methods: A total of 120 coated esthetic archwires (Teflon and Epoxy Resin) were investigated. They were divided into 4 groups and 8 subgroups. Cumulative discoloring effect of staining agents (aerated drink artificial orange, colgate plax mouth wash, and red wine) and control group in distilled water were analyzed for a period of 21 days and the solution was changed every 7th day. Samples were analyzed with spectrophotometer to obtain the L*, a*, and b* (lightness, red-green, and yellow-blue) color readings. Using these values total color change (?E*) at each level was also calculated. A general linear model (ANOVA and Tukey’s Post Hoc) test was used for statistical comparisons. Results: Significant differences were observed in ?E value of teflon and epoxy resin coated esthetic archwires between the staining agent and the control groups (p?.0001). Among the teflon coated esthetic archwires the ?E value for Artificial orange (aerated drink) (5.61±0.30) was highest indicating that artificial orange discolored the wires most in the inter group followed by the red wine (5.44±0.25), and colgate plax mouthwash (1.31±0.15) the least. Among the epoxy resin coated esthetic archwires the ?E value for red wine (4.52±0.30) was highest indicating that wires in the red wine discolored most. The colgate plax mouthwash (3.54±0.15) and the artificial orange (3.42±0.14) almost stain same. On overall comparision teflon coated and epoxy resin coated, the mean ?E value was statically significant in all the experimental groups. But the epoxy resin coated esthetic archwires were less stained as compare to Teflon coated wires (p value

Dentin erosion preventive effects of various plant extracts: An in vitro atomic force microscopy, scanning electron microscopy, and nanoindentation study.

The overall consumption of acidic beverages has become more common, making the prevention and treatment of dental erosion an important consideration. The aim of this in vitro study was to evaluate the effect of various plant extracts in preventing dentin erosion. Seven experimental groups (fluoride-free water, fluoride-containing mouthwash [Colgate Plax], green tea, rosehip, clove, pomegranate, and grape seed) were formed, each consisting of 20 bovine dentin samples. The specimens were exposed daily to demineralization and remineralization cycles three times per day over 5 days through a 5-min plant extract application before each erosive episode. Surface roughness, nanohardness values, and morphological changes on dentin surfaces were examined using atomic force microscopy (AFM), nanoindentation, and scanning electron microscopy (SEM). The data were subjected to Kruskal-Wallis and Friedman tests (p < .05). There were statistically significant differences between the groups in terms of nanohardness values (p < .05), except for the pomegranate and grape seed groups. The highest nanohardness value was observed in the clove group (1.24 ± 0.34 GPa), whereas the lowest nanohardness value was noted in the grape seed group (0.20 ± 0.04 GPa). The nanohardness values of positive and negative control groups after erosion cycles were statistically higher than the initial nanohardness values (p < .05).There was no statistically significant difference between the groups in surface roughness values (p > .05). Macromolecular deposits were observed both in the SEM and AFM images of the pomegranate, Colgate, and rosehip groups. It can be concluded that the clove extract group is more successful in preventing dentin erosion than the other groups. Green tea is also effective in preventing dentin erosion, similar to clove extract. The application of plant extracts may be a new treatment strategy in preventing dentin erosion. Plant extracts may also reduce the severity of existing dentin erosion. Clove and green tea extracts may present novel natural therapy potential by inhibiting dentin erosion.

Antimicrobial efficacy of Colgate Plax Cool Mint® mouthwash – in vivo studies

Abstract Hygienic procedures in oral cavity, both teeth brushing and using mouthwashes, have an influence on the composition and quantity of oral microflora. The aim of the work was to evaluate the impact of regular use of selected mouthwash on the titer of Lactobacillus and Streptococcus bacteria and Candida yeast-like fungi in the saliva. The evaluation of the influence of Colgate Plax Cool Mint® mouthwash on the number of Lactobacillus and Streptococcus bacteria and Candida yeast-like fungi in the saliva was done. The research included 39 persons at the age of 17±3 months, out of whom 30 people constituted a study group using mouthwash and 9 people – a control group not using mouthwash. The persons from the study group used the mouthwash twice a day for 2–3 minutes for the period of 5 months. The material for the microbiological tests constituted 2 ml of saliva collected three times from the participants: at the beginning of the research – before the use of a mouthwash and respectively, after 2.5 and 5 months from the first test. Colgate Plax Cool Mint®, the most frequently used by the youth in their daily oral hygiene and used in the present study mouthwash, showed effectiveness in reduction of Lactobacillus spp. and S. mutans bacteria, as well as C. albicans yeasts in the saliva of the studied persons, what is undoubtedly connected with inhibition of formation and prevention of oral plaque development.

In vitro tooth whitening effectiveness of whitening mouth rinses

Regardless of the extensive availability of mouth rinses that claim to whiten teeth, evidence of achievement of such effect is still missing. Aim: Therefore, this study assessed in vitro the whitening effectiveness of whitening mouth rinses. Methods: Sixty intact bovine incisors were embedded in acrylic resin and had their buccal surface flattened and polished. Then, the specimens were randomly allocated to three conventional (Colgate Plax, Cepacol and Listerine Cool Mint) and three whitening mouth rinse groups (Colgate Luminous White, Cepacol Whitening and Listerine Whitening Extreme) (n=10). Following, the specimens were immersed twice a day in the mouth rinses for one minute for 28 days. In between each immersion period, the specimens remained in artificial saliva at 37oC. Color was measured at baseline, 7, 14, 21, and 28 days using a portable spectrophotometer (Easyshade, Vita Zahnfabrik, Germany) with a 6 mm of diameter probe. Color change was analyzed considering the parameters of ∆L*, ∆a*, ∆b* and, ultimately, ∆E*. The whitening efficacy of the mouth rinses was analyzed using the Whiteness Index for Dentistry (WID). Data of ∆s was analyzed using two-way ANOVA and Tukey’s test (α=0.05). Results: The type of mouth rinse affected significantly all the ∆ parameters (p&lt;0.05). A non-whitening (conventional) mouth rinse produced the highest ΔE*, followed by the three whitening mouth rinses. The application time also affected ΔE* (p&lt;0.05), with emphasis on the third week of treatment. Only the hydrogen peroxide-containing mouth rinse (Listerine Whitening Extreme) presented a whitening effect, with an increasing trend over time. Conclusion: Although the overall color change was not different when comparing conventional and whitening mouth rinses, the hydrogen peroxide-containing whitening mouth rinse produces an increasing whitening trend over time. Not every mouth rinse that claims to whiten teeth produces the desired effect.

Effect of mouthwashes on the discoloration of bracket-bonded tooth surfaces: an in vitro study.

This in vitro study aimed to investigate the color changes of the bracket-bonded tooth surfaces after the use of 4 different mouthwashes. A total of 100 human premolar teeth were randomly divided into 10 equal groups. Color values (L*a*b*) of the buccal surfaces of each tooth were assessed using a digital spectrophotometer. Then the brackets were bonded. The groups were put either in sterile saline (4 test+1 control) or artificial saliva (4 test+1 control) solutions, and test groups were immersed in their mouthwashes (Colgate Plax, Listerine Cool Mint, Klorhex, and Tantum Verde) for 1min each in the morning/evening to simulate the mouth washing for 21days after the bonding. After the debonding and finishing procedures, final color measurements were performed. Color changes (∆E) were calculated. All of the parameters showed statistically significant differences among the groups. The least noticeable color changes were detected in the control groups. The most noticeable color change (ΔE) was observed in the Tantum Verde + artificial saliva group, followed by Tantum Verde + sterile saline and Klorhex + artificial saliva groups, all of which were significantly higher than the control groups. The use of mouthwashes during orthodontic treatment may cause noticeable changes in tooth color. Listerine Cool Mint may be the choice of mouthwash with its less discoloring effects. Artificial saliva should be preferred in similar in vitro studies to reflect the oral environment better. Mouthwash use during orthodontic treatment may result in different levels of enamel discoloration.

Effect of mouth rinses on tooth enamel surface

This study aims to evaluate the effect of mouth rinses on the color, microhardness, and surface roughness of tooth enamel. Sixty dental blocks were collected from human third molars and divided in five groups (n = 12): the control group (CG) without immersion in mouth rinse, Listerine Zero, Colgate Plax Fresh Mint, Listerine Whitening, and Colgate Luminous White. The groups were subjected to initial color analysis; the microhardness and roughness of the enamel surfaces were evaluated. Next, the samples were subjected to immersion in mouth rinses or brushing with conventional fluoride toothpaste (CG) according to the manufacturer's instructions; after a 12-week treatment, the color, microhardness, and roughness were once again assessed and compared with the initial analysis. Data were tabulated and analyzed through a two-way analysis of variance (ANOVA) (color and roughness) followed by Tukey's test. Microhardness was analyzed through the Kruskal-Wallis test followed by Dunn's test; the level of significance was 5%. All groups immersed in mouth rinses had a higher level of microhardness loss than CG; additionally, all groups showed changes in the enamel surface. Enamel surface loss was observed using a roughness test, and the mouth rinses promoted a higher level of color changes than CG. Given the results, it can be concluded that the mouth rinses led to significant changes in tooth enamel.

Formation of Dental Plaque Biofilm on Different Titanium Surfaces and Evaluate Antimicrobial Effects of Mouthrinses on Dental Plaque Biofilm

The aim of this study was to evaluate the effect of the morphology of titanium implant surfaces on dental plaque biofilm formation and the antimicrobial effects of mouthrinses on dental plaque biofilms regarding these titanium surfaces by using an open biofilm model. The average surface roughness (RA) of three types of titanium surfaces (Smooth, hydroxyapatite (HA), sandblast large grit and acid-etching (SLA)) were tested by atomic force microscope (AFM). Subgingival plaques were collected and cultured on titanium surfaces for 4 hours to 2 weeks. After treatment with mouthrinses, characterization of dental plaque biofilms was tested by field-emission SEM (FESEM) and confocal laser scanning microscopy (CLSM). The results of AFM and SEM showed that the surface roughness and biofilm thickness of HA and SLA surfaces were significantly higher than those of smooth surface. In addition, it was revealed that the mouthrinses were effective on the killing of young dental plaque biofilms, while the more mature biofilm (14-day-old) exhibited a stronger resistance to mouthrinses used in this study. In conclusion, the roughness of titanium surfaces can affect the dental plaque biofilm formation and Colgate Plax and Listerine COOL MINT are effective mouthrinses to kill dental plaques at the early stage of biofilm growth on the titanium implant surfaces.