This paper reports on experimental comparisons of coefficient of performance (COP) of a thermoelectric coolingsystem which cooled the hot side of the TEC by water (wc), ethylene glycol (egc) and nanofluids (nfc) The nanofluids is composed of ethylene glycol with silver nano(35 nm).The TEC was composed of the TE cooling modules, heat exchanger, and the air cooled heat sink at the cold side of the TE modules.Experiments were conducted with various current input 1 - 4.5 A to find out the optimum current input condition. To consideration of cooling capacity and COP of system was measured the hot and cold side temperature of TEC. Results shown that, the cooling capacity was increased with current input. The maximum cooling capacity of nfc, egc and wc are about 72, 62 and 41 W, respectively. Considered with highest COP found that the optimum current input is approximately 2.5 A. The maximum COP of nfc, egc and wc are about 2.01, 1.7 and 1.12, respectively. Therefore, the proposed TEC-nfc concept is expected to contribute to wider applications of the TE cooling system.