Coix Lacryma-jobi Research Articles

Coix Sprouts Affect Triglyceride Metabolism in Huh7 Cells and High-Fat Diet-Induced Obese Mice.

Lipolysis is the hydrolysis of triglycerides (TGs), commonly known as fats. Intracellular lipolysis of TG is associated with adipose triglyceride lipase (ATGL), which provides fatty acids during times of metabolic need. The aim of this study was to determine whether Coix lacryma-jobi L. var. ma-yuen Stapf (Coix) sprouts (CS) can alleviate obesity through lipolysis. Overall, we investigated the potential of CS under in vitro and invivo conditions and confirmed the underlying mechanisms. Huh7 cells were exposed to free fatty acids (FFAs), and C57BL/6J mice were fed a 60% high-fat diet. When FFA were introduced into Huh7 cells, the intracellular TG levels increased within the Huh7 cells. However, CS treatment significantly reduced intracellular TG levels. Furthermore, CS decreased the expression of Pparγ and Srebp1c mRNA and downregulated the mutant Pnpla3 (I148M) mRNA. Notably, CS significantly upregulated ATGL expression. CS treatment at a dose of 200 mg/kg/day resulted in a significant and dose-dependent decrease in body weight gain and epididymal adipose tissue weight. Specifically, the group treated with CS (200 mg/kg/day) exhibited a significant modulation of serum lipid biomarkers. In addition, CS ameliorated histological alterations in both the liver and adipose tissues. In summary, CS efficiently inhibited lipid accumulation through the activation of the lipolytic enzyme ATGL coupled with the suppression of enzymes involved in TG synthesis. Consequently, CS show promise as a potential anti-obesity agent.

Physicochemical and nutritional profiles of wild adlay (Coix lacryma-jobi Linn) accessions by GC, FTIR, and spectrophotometer

Purpose of current study was to determine physicochemical, triglyceride composition, and functional groups of wild adlay accessions (brown, black, yellow, grey, green, off white, and purple) to find out its scope as cereal crop. Triglycerides, minerals and functional groups were determined through Gas chromatography, spectrophotometer and Fourier Transform Infrared (FTIR) spectrophotometer respectively. Results revealed variation among bulk densities, specific densities, percent empty spaces, and corresponding grain counts per 10 g of sample are useful in distinguishing brown, black, yellow, grey, green, off white, and purple wild adlay accessions. Specific density and grain count per 10 g sample was significantly related. No statistical relationship exists among the pronounced physical characteristics. Brown adlay expressed the highest protein, fat, and fiber contents 15.82%, 4.76% and 2.37% respectively. Protein, fat, ash, and fiber percent contents were found comparable to cultivated adlay. Spectrophotometric analysis revealed macro elements including phosphorus, potassium, calcium, and sodium in the range 0.3% - 2.2% and micro elements boron, iron, copper, zinc, and manganese in the range 1.6 mg/kg - 20.8 mg/kg. Gas chromatography showed polyunsaturated fatty acids (PUFA) constitute the primary fraction (39% ± 7.2) of wild adlay triglycerides. Linoleic and palmitic acids were present as prominent fatty acids, 43.5% ±1.4 and 26.3% ±1.4 respectively. Infra-red frequencies distinguished functional groups in narrow band and fingerprint region of protein in association with out of plane region leading to structural differences among adlay accessions. Comparison of major distinguishing vibrational frequencies among different flours indicated black adlay containing highest functional groups appeared promising for varietal development.

Growth and yield response of Watani Wado job's tears (Coix lacryma-jobi L.) to the application frequency of phosphate fertilizer and paclobutrazol in different growing seasons

Job's tears (Coix lacryma-jobi L.) is a carbohydrate source functional food crop that can be developed as a substitute for rice. This study aims to determine the best application frequency of phosphate (P) fertilizer and paclobutrazol for supporting the growth and yield of job's tears in both rainy and dry seasons. The research was conducted from November 2021 - November 2022 at the Ciparanje research station and Laboratory Centre Universitas Padjadjaran, Jatinangor, Sumedang. The experiment used a randomized block design (RBD) consisting of P fertilizer application frequency (once, twice, and thrice) and paclobutrazol application frequency (once, twice, and thrice) and three replications.. The best season was determined using the T-test. The results showed that there was no interaction between growth components and job's tears yield in both rainy and dry season planting. P fertilizer application had a significant effect on lateral branch per tiller in the rainy season and root-shoot ratio in the dry season. Paclobutrazol application had a significant effect on the number of tillers per plant and number of seeds per plant in the rainy season, and the lateral branch per tiller in the dry season. Planting in the rainy season had the best growth and yield components compared to the dry season.

Construction of a high-density genetic map based on large-scale marker development in Coix lacryma-jobi L. using specific-locus amplified fragment sequencing (slaf-seq)

Coix lacryma-jobi L. is one of the most economically and medicinally important corns. This study constructed a high-density genetic linkage map of C. lacryma-jobi based on a cross between the parents 'Qianyi No. 2' × 'Wenyi No. 2' and their F2 progeny through high-throughput sequencing and the construction of a specific-locus amplified fragment (SLAF) library. After pre-processing, 325.49 GB of raw data containing 1628 M reads were obtained. A total of 22,944 high-quality SLAFs were identified, among which 3952 SLAFs and 3646 polymorphic markers met the requirements for the construction of a genetic linkage map. The integrated map contained 3605 high-quality SLAFs, which were grouped into ten genetic linkage groups. The total length of the map was 1620.39 cM, with an average distance of 0.45 cM and an average of 360.5 markers per linkage group. This report presents the first high-density genetic map of C. lacryma-jobi. This map was constructed using an F2 population and SLAF-seq approach, which allows the development of a large number of polymorphic markers in a short period. These results provide a platform for precise gene/quantitative trait locus (QTL) mapping, map-based gene separation, and molecular breeding in C. lacryma-jobi. They also help identify a target gene for tracking, splitting quantitative traits, and estimating the phenotypic effects of each QTL for QTL mapping. They are of great significance for improving the efficiency of discovering and utilizing excellent gene resources of C. lacryma-jobi.

Effects of storage methods and packaging materials on viability of Adlai (Coix lacryma-jobi L.) Seeds

Effects of storage methods and packaging materials on viability of Adlai (Coix lacryma-jobi L.) Seeds

Functionally graded structures in the involucre of Job’s tears

Nature is filled with materials that are both strong and light, such as bones, teeth, bamboo, seashells, arthropod exoskeletons, and nut shells. The insights gained from analyzing the changing chemical compositions and structural characteristics, as well as the mechanical properties of these materials, have been applied in developing innovative, durable, and lightweight materials like those used for impact absorption. This research concentrates on the involucres of Job’s tears (Coix lacryma-jobi var. lacryma-jobi), which are rich in silica, hard, and serve to encase the seeds. The chemical composition and structural characteristics of involucres were observed using scanning electron microscopy and energy-dispersive x-ray spectroscopy and optical microscopy with safranin staining. The hardness of the outer and inner surfaces of the involucre was measured using the micro-Vickers hardness test, and the Young’s modulus of the involucre’s cross-section was measured using nanoindentation. Additionally, the breaking behavior of involucres was measured through compression test and three-point bending tests. The results revealed a smooth transition in chemical composition, as well as in the orientation and dimensions of the tissues from the outer to the inner layers of involucres. Furthermore, it was estimated that the spatial gradient of the Young’s modulus is due to the gradient of silica deposition. By distributing the hard, brittle silica in the outer layer and elastoplastic organic components in the middle and inner layers, the involucres effectively respond to compressive and tensile stresses that occur when loads are applied to the outside of the involucre. Furthermore, the involucres are reinforced in both meridional and equatorial directions by robust fibrovascular bundles, fibrous bundles, and the inner layer’s sclerenchyma fibers. From these factors, it was found that involucres exhibit high toughness against loads from outside, making it less prone to cracking.

Genetic diversity and population structure analysis in Job's tears (Coix lacryma-jobi L.) collections from northeastern Himalayan region

Genetic diversity and population structure analysis in Job's tears (Coix lacryma-jobi L.) collections from northeastern Himalayan region

Exploring the relationship between seed weight and seed coat characteristics in the adlay (Coix lacryma-jobi L.) cultivar WATANI-2 through N, P and K treatment compositions

Adlay plant (Coix lacryma-jobi) grown in many Asian countries, as a source of carbohydrates. The cultivation of adlay faces challenges such as long growth periods, low yields, and hard seeds. Efforts to enhance productivity are crucial, specifically by applying Nitrogen, Phosphorus, and Potassium fertilizers at the appropriate and balanced doses that have not yet been established. Therefore, this study aimed to investigate the relationship between seed weight and seed coat characteristics under optimal N, P, and K fertilizer doses. The exploration process was conducted at the Research Station and Plant Production Technology Laboratory, Faculty of Agriculture, Padjadjaran University, from April 2019 to November 2020. Features of the location included an altitude of 750 – 800 m above sea level, a climate type C3 (Oldeman), an average temperature of 23.8oC, and a soil classified as Inceptisols with a pH of 5.83. The experimental design incorporated several treatments at minimum, optimum, and maximum dosage levels, namely N (90kg/ha, 180 kg/ha, and 270 kg/ha), P (37.5kg/ha, 75 kg/ha, and 150 kg/ha), and K (60 kg/ha, 120 kg/ha and 240 kg/ha) fertilizers, each was repeated twice. Fertilizer application was administered at the beginning of growth and the late vegetative phase, while P was given during the late vegetative phase and before seed formation. Subsequently, a regression analysis was performed to determine the relationship of the weight of 100 seeds with thickness and hardness, generating the equation: Y = 13.755 + 0.061 X1 + 0.012 X2, where X1 = seed hardness and X2 = seed coat thickness. Observations were carried out on the weight, thickness, and hardness of adlay seeds, both macroscopically by measuring length and width or diameter and microscopically through the evaluation of coat and silica layer thickness). The results showed a significant relationship between seed weight, thickness, and hardness, yielding 5.56 t/ha with an 80% land efficiency.

Sensory Acceptability and Nutritional Content of Cereal Bars Formulated with Adlay (Coix lacryma-jobi L.) Grains

In the Philippines, an underutilized crop known as adlay has been found to contain valuable nutritive potential. Given Filipinos' usual diet of snacks and ready-to-eat food items, the study aimed to provide an alternative choice for healthy snacks by utilizing adlay in developing cereal bars with an improved nutritional profile. The study developed cereal bars using varying proportions of oats and adlay grains as follows: control (100:0), treatment 1 (75:25), treatment 2 (50:50), and treatment 3 (25:75). The cereal bars were then subjected to sensory evaluation to determine their acceptability using a nine-point hedonic scale with 67 adult respondents. The control and the most acceptable treatment then underwent proximate and mineral analyses (iron and calcium). The sensory data was analyzed using Kruskal-Wallis H-Test with rank analysis, whereas the proximate and mineral content data were analyzed using the student's t-test at p<0.05. Results showed no significant difference between the control and all the treatments regarding sensory characteristics, and treatment 1 got the highest rank among all treatments. Proximate and mineral analyses revealed that treatment 1 has significantly higher crude protein (11.25 ± 0.66\%), crude fiber (10.79 ± 1.22\%), crude fat (17.82 ± 1.10\%), and calcium (1.08 ± 0\%), than the control made of pure oats. The findings, therefore, suggest that adlay can be used as a value-added ingredient in making sensory-acceptable cereal bars with an improved nutritional profile.

Effects of Storage Methods and Packaging Materials on Viability of Adlai (Coix lacryma-jobi L.) Seeds

Effects of Storage Methods and Packaging Materials on Viability of Adlai (Coix lacryma-jobi L.) Seeds

Interactive effects of NPK fertilizer and paclobutrazol concentration on growth and yield of hanjeli (Coix lacryma-jobi L.)

The balance between growth and crop yield can be manipulated by exploiting nutrient and retardant interactions. This study is aiming to assess the effects of various combinations of NPK 16-16-16 fertilizer in different doses, along with the application of multiple concentrations of paclobutrazol on the growth and yield of hanjeli. This experiment was carried out in the experimental field of Ciparanje, Faculty of Agriculture Universitas Padjadjaran, Jatinangor, Sumedang from February 2021 to August 2021. This research was conducted in a split-plot design with three replications. The main plot consisted of 200 kg/ha (n1), 250 kg/ha (n2), and 300 kg/ha (n3). The subplot consisted of 2000 ppm (p1), 3000 (p2), and 4000 ppm (p3). The results show that there were interaction effects between different doses of NPK fertilizer and paclobutrazol concentrations on plant height and 100-grain weight. The best combination came from the application of 200 kg/ha NPK fertilizer with 3000 ppm of paclobutrazol, which gave higher results in plant height and 200 kg/ha NPK fertilizer with 2000 ppm of paclobutrazol on 100-grain weight.

Spatial O2 Profile in Coix lacryma-jobi and Sorghum bicolor along the Gas Diffusion Pathway under Waterlogging Conditions.

While internal aeration in plants is critical for adaptation to waterlogging, there is a gap in understanding the differences in oxygen diffusion gradients from shoots to roots between hypoxia-tolerant and -sensitive species. This study aims to elucidate the differences in tissue oxygen concentration at various locations on the shoot and root between a hypoxia-tolerant species and a -sensitive species using a microneedle sensor that allows for spatial oxygen profiling. Job's tears, a hypoxia-tolerant species, and sorghum, a hypoxia-susceptible species, were tested. Plants aged 10 days were acclimated to a hypoxic agar solution for 12 days. Oxygen was profiled near the root tip, root base, root shoot junction, stem, and leaf. An anatomical analysis was also performed on the roots used for the O2 profile. The oxygen partial pressure (pO2) values at the root base and tip of sorghum were significantly lower than that of the root of Job's tears. At the base of the root of Job's tears, pO2 rapidly decreased from the root cortex to the surface, indicating a function to inhibit oxygen leakage. No significant differences in pO2 between the species were identified in the shoot part. The root cortex to stele ratio was significantly higher from the root tip to the base in Job's tears compared to sorghum. The pO2 gradient began to differ greatly at the root shoot junction and root base longitudinally, and between the cortex and stele radially, between Job's tears and sorghum. Differences in the root oxygen retention capacity and the cortex to stele ratio are considered to be related to differences in pO2.

Study of chemical, physical stabilites, and sensory properties of sorghum-adlay-based milk

Plant-based beverages made from various plant sources have been regarded as a good replacement for animal-based milk, especially for people with health limitations and customary food practices such as vegans and vegetarians. The development of alternative beverages made from sorghum (Sorghum bicolor L.) and adlay (Coix lacryma-jobi L.) was limited in Indonesia, despite the grains have been reported to contain good nutrients and health-benefiting compounds. Therefore, the aim of the research was to create plant-based beverages made from sorghum and adlay and evaluate their nutritional value, physical stability, and consumer acceptance. There were five formulas tested in this research, namely formula 1 (100% sorghum, 0% adlay), formula 2 (90% sorghum, 10% adlay), formula 3 (80% sorghum, 20% adlay), formula 4 (70% sorghum, 30% adlay), and formula 5 (60% sorghum, 40% adlay). The results showed that the addition of adlay to the formulation of a sorghum-adlay-based beverage significantly increased the carbohydrate content from 1.24% to 2.71%, tended to increase the protein content from 0.27% to 0.33%, decreased moisture content from 98.47% to 96.91%, and decreased sodium and potassium significantly. The higher concentration of adlay in the product significantly reduced the physical stability of the product and decreased its sensory acceptance. Based on sensory analysis, formula 3 was chosen as the most acceptable sorghum-adlay-based milk with a score of 4.9. The data obtained give brief information on the development of sorghum-adlay-based milk.

New insights into the key role of node I in thallium accumulation in seed of coix (Coix lacryma-jobi L.)

The mechanisms underlying the distribution of many toxic metal(loid)s in shoots and metal(loid) transport to grains have been well documented in the quest for food safety but there remains a lack of knowledge on thallium (Tl) accumulation in food crops. Here, field investigations combined with a glasshouse pot experiment were conducted to investigate the characteristics of Tl distribution and accumulation in coix, a major food crop in south Guizhou province, China, and the role of node I in restricting Tl transport to the seed. Fourteen percent of coix seed samples collected from the Lanmuchang Tl-As-Hg mine contained higher Tl concentrations than the recommended limit for foods and feedstuffs in Germany (0.5 mg kg−1), with the highest exceedance rate of the metal(loid)s determined, when grown in soils surrounding the mine with a very high Tl concentration of 0.07–89.5 mg kg−1 and a general low pH of 4.19–6.48. Thallium concentrations were higher in coix nodes than in internodes, followed by roots and grains. The Tl translocation factors from node I to grains were 0.01–0.21 and were the lowest of any translocation factors between different tissues. Node I is therefore the key tissue restricting Tl transport to coix grains. Thallium was localized mainly in the diffuse vascular bundles (DVBs) in node I. The co-localization of Tl and sulfur in the DVBs and Tl contamination-induced phytochelatin (PC) accumulation indicate that Tl storage in the DVBs involving complexation with PCs in node I is an important process in Tl accumulation in coix grains. Moreover, the area of DVBs in node I increased with increasing soil Tl pollution level, providing more channels for Tl transport to the panicles and grains and thereby acting as a key factor restricting Tl transport to the grains. These results provide new insights into the key role of node I in Tl accumulation in coix grains and indicate key points to minimize Tl accumulation in grains for food safety.

Integrated application of morphological, anatomical, biochemical and physico-chemical methods to identify superior, lignocellulosic grass feedstocks for bioenergy purposes

Grasses have sufficient potential to be used in the biofuel industry due to many reasons. Hence, systematic assessments of the lignocellulosic biomass obtained from six grasses namely Arundo donax, Chrysopogon zizanioides, Coix lacryma-jobi, Pennisetum purpureum, Saccharum spontaneum, and Sorghum bicolor have been performed to assess their potential for bioethanol production. Selected morphological (height, internode diameter, number of internodes, fresh biomass, dry biomass, total volatile matter), anatomical (vascular bundle, stain intensity), analytical (crystallinity index of cellulose, thermal stability, hemicellulose, moisture content) and biochemical (α-cellulose, acid insoluble lignin) parameters were investigated and statistically analysed to detect the superior grass species. The study indicates that S. spontaneum harbours promising biofuel potential by having high dry biomass (56.33 g), α-cellulose (55.88–67.76%), but lower thermal stability (Tmax: 344.37 °C). Comparable ligno-cellulose properties with respect to dry biomass (54.60 g), α-cellulose content (45.95–64.14%), Tmax (360.51 °C) have been observed for A. donax. However, relatively higher lignin contents (∼17–23%) and crystalline cellulose (43.63%–46.96%) in S. spontaneum and A. donax might have resulted into lesser saccharification yield (216.04–230.08 mg/g). On that aspect, S. bicolor and C. lacryma-jobi might be useful since they possessed lower lignin (14.03–16.56%) and crystalline cellulose (32.51–33.02%) leading to higher glucose yield (263–420.06 mg/g). Taken together, this study indicates that integration of morphological, biochemical, and analytical properties of biomasses are complex, yet useful to predict a ‘superior donor plant’ for bioenergy purposes.

Morphological characteristics and variability of traditional starch forming coix (Coix lacryma-jobi var. ma-yuen) populations from Mindanao Island, Philippines

Abstract. Bon SG, Antesco GP, Enicola EE. 2023. Morphological characteristics and variability of traditional starch forming coix (Coix lacryma-jobi var. ma-yuen populations from Mindanao Island, Philippines. Biodiversitas 24: 4382-4391. Adlay (Coix lacryma-jobi var. ma-yuen (Rom.Caill.) Stapf) is an underutilized crop in the Philippines with high potential. Characterization and elucidation of the available variability among the local adlay populations are essential information for its improvement and better utilization. The study aimed to characterize the qualitative morphological traits of 34 traditional adlay populations from Mindanao Provinces and assess diversity by the Shannon Index and similarity by multivariate clustering. Results showed that all populations shared the same morphotypes in five descriptors but were variable for the rest. Six populations showed intense leaf anthocyanin coloration, and 23 expressed upright growth habits. Three types of leaf blade colors were observed, while the intensity of anthocyanin coloration of culms was expressed in four types. Most populations had semi-pubescent or highly pubescent leaves, generally intermediate leaf orientation and non-glaucous leaves. Immature grains were generally light green but expressed four color types at maturity, mostly circular with furrows on the surface. Diversity Indices (H') ranged from low to high. Leaf blade pubescence, anthocyanin coloration of culm, and mature grain color had high H' values. Cluster analysis showed that the variability was narrow, where overall clustering achieved 0.50 similarity. At 0.65 similarity, three groups can be derived with three populations as outliers. Clustering can be attributed to the differences in seedling color, anthocyanin coloration, plant growth habit, immature grain color, mature grain color, and shape. The study established the morphological description of the qualitative traits of traditional Philippine adlay and assessed diversity to be generally intermediate, where populations can be clustered into subgroups according to the degree of morphotype similarities. Clustering, however, was not related to provenances. It is recommended to conduct further geographic germplasm sampling, marker-based characterization, and agronomic evaluation to dissect further the genetic diversity and crop potential of the local adlay populations.

Effects of husk charcoal and watering on the biochemical and physiological properties of coix millet (Coix lacryma jobi L.) during vegetative phase

Continuous climate change increases the number of droughts in some areas, thereby affecting agricultural production. Cultivation of coix millet (Jali plant in Indonesian) is profitable because of its high adaptability to drought stress, and its seeds have the potential to be used as food due to their benefits for body health. The application of organic matter, namely husk charcoal, was chosen to increase agricultural production under drought stress. Therefore, this study aimed to determine the effects of husk charcoal on the biochemical and physiological properties of coix millet during vegetative phase under drought stress and to determine the longest watering interval the plant can tolerate. Research was arranged in a split-plot design with three replications. The main plot consisted of three watering intervals (every two, four, and six days). The sub-plots consisted of three doses of husk charcoal, namely 0 g polybag⁻¹ (0 tons ha⁻¹), 32 g polybag⁻¹ (10 tons ha⁻¹), and 64 g polybag⁻¹ (20 tons ha⁻¹). The data were analyzed using analysis of variance (ANOVA) and tested using Duncan's Multiple Distance Test (DMRT) at a significant level of 5%. The results showed that husk charcoal could increase the chlorophyll content in plants experiencing drought stress. The longest watering interval that could be tolerated by coix millet given husk charcoal at the optimal dose (64 g polybag⁻¹) was once every four days.

Genome-wide analysis of the HSP20 gene family and its response to heat and drought stress in Coix (Coix lacryma-jobi L.)

BackgroundHeat shock protein 20 (HSP20) is a member of the heat stress-related protein family, which plays critical roles in plant growth, development, and response to abiotic stresses. Although many HSP20 genes have been associated with heat stress in numerous types of plants, little is known about the details of the HSP20 gene family in Coix. To investigate the mechanisms of the ClHSP20 response to heat and drought stresses, the ClHSP20 gene family in Coix was identified and characterized based on genome-wide analysis.ResultsA total of 32 putative ClHSP20 genes were identified and characterized in Coix. Phylogenetic analysis indicated that ClHSP20s were grouped into 11 subfamilies. The duplicated event analysis demonstrated that tandem duplication and segment duplication events played crucial roles in promoting the expansion of the ClHSP20 gene family. Synteny analysis showed that Coix shared the highest homology in 36 HSP20 gene pairs with wheat, followed by 22, 19, 15, and 15 homologous gene pairs with maize, sorghum, barley, and rice, respectively. The expression profile analysis showed that almost all ClHSP20 genes had different expression levels in at least one tissue. Furthermore, 22 of the 32 ClHSP20 genes responded to heat stress, with 11 ClHSP20 genes being significantly upregulated and 11 ClHSP20 genes being significantly downregulated. Furthermore, 13 of the 32 ClHSP20 genes responded to drought stress, with 6 ClHSP20 genes being significantly upregulated and 5 ClHSP20 genes being significantly downregulated.ConclusionsThirty-two ClHSP20 genes were identified and characterized in the genome of Coix. Tandem and segmental duplication were identified as having caused the expansion of the ClHSP20 gene family. The expression patterns of the ClHSP20 genes suggested that they play a critical role in growth, development, and response to heat and drought stress. The current study provides a theoretical basis for further research on ClHSP20s and will facilitate the functional characterization of ClHSP20 genes.

Comparison of Quality, Antioxidant Capacity, and Anti-Inflammatory Activity of Adlay [Coix lacryma-jobi L. var. ma-yuen (Rom. Caill.) Stapf.] Sprout at Several Harvest Time.

Recently, there has been a growing interest in the consumption of plant-based foods such as vegetables and grains for the purpose of disease prevention and treatment. Adlay seeds contain physiologically active substances, including coixol, coixenolide, and lactams. In this study, adlay sprouts were cultivated and harvested at various time points, specifically at 3, 5, 7, 9, and 11 days after sowing. The antioxidant activity of the extracts was evaluated using assays such as DPPH radical scavenging, ABTS radical scavenging, reducing power, and total polyphenol contents. The toxicity of the extracts was assessed using cell culture and the WST-1 assay. The aboveground components of the sprouts demonstrated a significant increase in length, ranging from 2.75 cm to 21.87 cm, weight, ranging from 0.05 g to 0.32 g, and biomass, ranging from 161.4 g to 1319.1 g, as the number of days after sowing advanced, reaching its peak coixol content of 39.38 mg/g on the third day after sowing. Notably, the antioxidant enzyme activity was highest between the third and fifth days after sowing. Regarding anti-inflammatory activity, the inhibition of cyclooxygenase 2 (COX-2) expression was most prominent in samples harvested from the ninth to eleventh days after sowing, corresponding to the later stage of growth. While the overall production mass increased with the number of days after sowing, considering factors such as yield increase index per unit area, turnover rate, and antioxidant activity, harvesting at the early growth stage, specifically between the fifth and seventh days after sowing, was found to be economically advantageous. Thus, the quality, antioxidant capacity, and anti-inflammatory activity of adlay sprouts varied depending on the harvest time, highlighting the importance of determining the appropriate harvest time based on the production objectives. This study demonstrates the changes in the growth and quality of adlay sprouts in relation to the harvest time, emphasizing the potential for developing a market for adlay sprouts as a new food product.

Ethnomedicinal knowledge used by Mullukurumbas of Nilgiris, Western Ghats, India

Abstract. Sanu C, Jeevith S, Sheeba TC. 2023. Ethnomedicinal knowledge used by Mullukurumbas of Nilgiris, Western Ghats, India. Asian J Ethnobiol 6: 118-129. Nilgiris is one of the biodiversity-rich areas in the Western Ghats, India, with six major groups of indigenous tribes, and Mullukuruba is one of the subtribes of Kurumbas. The present study aimed to document the ethnomedicinal knowledge among the Mullukurumba Tribe of Kallical, Cherangode Village of Nilgiris, Western Ghats. Most of the tribes in Nilgiris follow a close cultural ritual and traditional knowledge, including food and medicine. The field data were collected through interviews, observation, and documentation. The tribal people utilized 152 plant species belonging to 60 families. The life forms of the collected plants revealed that herbs constitute 53 species, followed by shrubs 44 species, trees 35 species, and climbers 20 species. Family-wise, Fabaceae dominated with 13 species, followed by Asteraceae 10 species, and Solanaceae included 8 species. The use category made by the tribal community were medicinal plants (70%), edible fruits (19%), and miscellaneous uses (11%). The plant species treat various illnesses, including piles, rheumatism, gynecological issues, dermatitis, and antidiarrheal and anti-diabetic conditions. Argyreia cuneata (Willd.) Ker Gawl., Cascabela thevetia (L.) Lippold, Coix lacryma-jobi L., Ixora coccinea L., Gloriosa superba L., Melastoma malabathricum L., and Plumeria alba L. were the most common plants used for rituals. Also, this study presented an updated checklist of medicinal plants used by the Mullukurumba Tribe.