Habitual Coffee Drinkers Research Articles

Habitual coffee drinking and the chance of prediabetes remission: findings from a population with low coffee consumption.

We aimed to investigate the association between coffee drinking and total caffeine intakes with the chance of prediabetes (Pre-DM) regression and progression over 9-years of follow-up. This cohort study included 334 Pre-DM individuals (mean age of 49.4 ± 12.8 years and 51.5% men) who participated in the third phase of the Tehran Lipid and Glucose Study (2006-2008). A validated food frequency questionnaire at baseline assessed habitual coffee consumption. All measurements were done at baseline and all subsequent examinations with 3-year follow-up intervals. The odds ratios (OR) and 95% confidence intervals (CIs) of Pre-DM regression to normal glycemia or progression to type 2 diabetes (T2D) in coffee drinkers/non-drinkers were estimated using multinomial logistic regression analysis. During the study follow-up 39.8% of the study participants were progressed to T2D and 39.8% returned to normal glycemia. Coffee consumption nearly doubled the chance of returning to normal (OR = 2.26, 95% CI = 1.03-4.97). Total caffeine intake was not related to Pre-DM progression and regression. Compared to non-drinkers, coffee drinkers had significantly lower 2-hour serum glucose concentrations over time (152, 95% CI = 144-159 vs. 162, 95% CI = 155-169mg/dL, P = 0.05). Habitual coffee drinking may increase the chance of returning to normal glycemia in Pre-DM subjects.

Genetic Factors Causing the Prevalence of Anemia in Young Girls and Stunting in Toddlers: A Systematic Literature Review

The prevalence of anemia in adolescents at the national level is still considered quite high. Several factors are associated with the incidence of anemia in female adolescents, namely energy intake, protein intake, iron intake, vitamin C intake, tea or coffee drinking habits, investment in worms, knowledge, education and type of parental occupation, family income, and menstrual patterns as well as genetics. Stunting is also still a problem in Indonesia due to insufficient nutritional intake for quite a long time. Stunting occurs when the fetus is still in the womb and only appears when the child is two years old. anemia caused by malnutrition at an early age increases infant and child mortality. anemia in young women and also stunting in children is very dangerous. Where the purpose of research is to explain Genetic Factors Causing the Prevalence of Anemia in Young Girls and Stunting in Toddlers. A review is conducted on the state-of-the-art methods using the preferred reporting items for reviews and meta-analyses (PRISMA) guidelines. We review literature from several publications and analyze genetic factors that cause the prevalence of anemia in young women. The prevalence of anemia among young women is also caused by genetic factors. Young women tend to experience anemia because during this period they experience growth and development. The risk of anemia increases with physiological shifts such as menstrual periods. To prevent anemia, the government has planned a program for the Prevention and Control of Iron Nutrition Anemia in women of childbearing age which aims to reduce the prevalence of iron deficiency anemia in high school and junior high school students.

Immediate effect of caffeine on sympathetic nerve activity: why coffee is safe? A single-centre crossover study

PurposesHabitual coffee drinking is ubiquitous and generally considered to be safe despite its transient hypertensive effect. Our purpose was to determine the role of the sympathetic nervous system in the hypertensive response.MethodsIn a single-centre crossover study, medical caregivers were studied after consumption of standard coffee (espresso), water and decaffeinated coffee (decaff) given in random order at least 1 month apart. Plasma caffeine levels, mean arterial pressure, heart rate, total peripheral resistance and muscle sympathetic activity were recorded. Baroreflex activity was assessed using burst incidence and RR interval changes to spontaneous blood pressure fluctuations.ResultsA total of 16 subjects (mean [± standard error] age 34.4 ± 2 years; 44% female) were recruited to the study. Three agents were studied in ten subjects, and two agents were studied in six subjects. Over a 120-min period following the consumption of standard coffee, mean (± SE) plasma caffeine levels increased from 2.4 ± 0.8 to 21.0 ± 4 µmol/L and arterial pressure increased to 103 ± 1 mmHg compared to water (101 ± 1 mmHg; p = 0.066) and decaff (100 ± 1 mmHg; p = 0.016). Peripheral resistance in the same period following coffee increased to 120 ± 4% of the baseline level compared to water (107 ± 4; p = 0.01) and decaff (109 ± 4; p = 0.02). Heart rate was lower after both coffee and decaff consumption: 62 ± 1 bpm compared to water (64 bpm; p = 0.01 and p = 0.02, respectively). Cardio-vagal baroreflex activity remained stable after coffee, but sympathetic activity decreased, with burst frequency of 96 ± 3% versus water (106 ± 3%; p = 0.04) and decaff (112 ± 3%; p = 0.001) despite a fall in baroreflex activity from – 2.2 ± 0.1 to – 1.8 ± 0.1 bursts/100 beats/mmHg, compared to water (p = 0.009) and decaff (p = 0.004).ConclusionThe hypertensive response to coffee is secondary to peripheral vasoconstriction but this is not mediated by increased sympathetic nerve activity. These results may explain why habitual coffee drinking is safe.

Coffee consumption decreases the connectivity of the posterior Default Mode Network (DMN) at rest.

Habitual coffee consumers justify their life choices by arguing that they become more alert and increase motor and cognitive performance and efficiency; however, these subjective impressions still do not have a neurobiological correlation. Using functional connectivity approaches to study resting-state fMRI data in a group of habitual coffee drinkers, we herein show that coffee consumption decreased connectivity of the posterior default mode network (DMN) and between the somatosensory/motor networks and the prefrontal cortex, while the connectivity in nodes of the higher visual and the right executive control network (RECN) is increased after drinking coffee; data also show that caffeine intake only replicated the impact of coffee on the posterior DMN, thus disentangling the neurochemical effects of caffeine from the experience of having a coffee.

A Study of Coronary Heart Patients’ Lifestyle at Zainoel Abidin Hospital, Banda Aceh, Indonesia

Background: About 40% of heart disease patients do not realize they have coronary heart disease (CHD). Based on the lifestyle of people in Aceh, several factors can increase the CHD risk, such as smoking, coffee, fatty foods, stress, and lack of physical activity. Such a lifestyle has the potential to increase the risk of CHD, as evidenced by statistical papers reporting high levels of CHD cases in Aceh. This study was aimed to review the patients’ lifestyle with coronary heart disease at Zainoel Abidin Hospital, Banda Aceh, Indonesia. Method: This research is quantitative, with a case-control design. In April 2017, 206 cases of CHD and 206 non-PJK cases were analyzed, using the patient’s medical record and a lifestyle questionnaire, in Zainoel Abidin Hospital. The result of bivariate analysis through a chi-square test showed that lifestyle factors related to CHD included diet, physical activity, smoking, coffee, and stress control (p<0,05). Result: The results of the multivariate analysis with logistic regression showed that smoking is a lifestyle that remains consistent as a risk factor for CHD. In addition, after interacting with other risk factors, stress control and coffee drinking habits become a protective factor of CHD. Smokers are twice risky to have CHD than nonsmokers. Conclusion: The influence of lifestyle on CHD is also determined by age and income factors. The research implications of this study offer various parties the opportunity to pursue active involvement in preventive efforts against CHD. Bangladesh Journal of Medical Science Vol. 22 No. 03 July’23 Page : 632-637

Influence of Sex Differences on Serum Lipid Profiles among Habitual Coffee Drinkers: Evidence from 23,072 Taiwan Biobank Participants.

The bioactive compounds of coffee are involved in lipid metabolism, and sex differences may play an important role. This study aimed to evaluate the influence of sex differences on serum lipid profiles among habitual coffee drinkers. We conducted a nationwide cross-sectional study of 23,628 adults using data obtained from the Taiwan Biobank database. Adults who drank more than one cup of coffee per day and those who drank less than one cup per day were compared with non-drinkers. After adjusting for baseline demographics and lifestyle, a generalized linear model was used to estimate the change in serum lipid profiles in men and women and in postmenopausal and premenopausal women among different coffee-drinking behaviors. We found that habitual coffee consumption changed the serum lipid profiles of men and women. Further, coffee drinkers had higher serum total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, and lower serum triglyceride levels than non-drinkers. Compared with premenopausal women, both men and postmenopausal women had increased serum total cholesterol and low-density lipoprotein cholesterol levels. Menopausal status may play an important role in modulating the effect of habitual coffee intake on dyslipidemia. Moreover, premenopausal women potentially benefit more from habitual coffee drinking than men and postmenopausal women.

The Association between Coffee and Tea Consumption at Midlife and Risk of Dementia Later in Life: The HUNT Study.

Studies exploring the possible protective effect of coffee and tea consumption on dementia have shown inconsistent results so far. We aimed to investigate whether consumption of tea and different types of coffee at midlife are associated with dementia later in life and whether sex or ApoE4 influence such association. We included 7381 participants from the Norwegian HUNT Study. Self-reported questionnaires assessed daily consumption of coffee and tea at baseline. After 22 years, individuals 70 years or older were screened for cognitive impairment. General coffee consumption and tea consumption was not associated with dementia risk. Compared to daily consumption of 0-1 cups of coffee, daily consumption of ≥8 cups of boiled coffee was associated with increased dementia risk in women (OR: 1.83, 95% CI: 1.10-3.04, p-value for trend = 0.03) and daily consumption of 4-5 cups of other types of coffee was associated with a decrease in dementia risk in men (OR: 0.48, 95% CI: 0.32-0.72, p-value for trend = 0.05). Furthermore, the association between boiled coffee and increased dementia risk was only found in ApoE4 non-carriers. Differences by sex or ApoE4 carrier status were not supported by strong statistical evidence for interaction. Tea consumption was not associated with dementia risk. type of coffee may play a role in the direction of the association between coffee-drinking habits and dementia later in life.

The Association of Smoking and Coffee Consumption With Occurrence of Upper Gastrointestinal Symptoms in Patients With Active Helicobacter pylori Infection in Jazan City: A Cross-Sectional Study.

Helicobacter pylori(H. pylori) is a severe infection responsible for upper gastrointestinal symptoms (UGISs). Several causes of H. pylori infection include food ingestion and person-to-person transmission. Many lifestyle variables can affect the occurrence of UGISssuch ascoffee consumptionand smoking. To assess the association between smoking and coffee consumption and the occurrence of UGISs in patients with active H. pylori infection in Jazan city in Saudi Arabia. A descriptive cross-sectional research design was used to conduct the study between July 2022 and August 2022 in Jazan, southern Saudi Arabia. Male and female Saudis or non-Saudis ≥ 18 years of age with an active H. pylori infection were included. Participants under 18 years or without active H. pylori infection were excluded. Data were collected from participants using the convenience sampling technique and a structured questionnaire. The first part of the questionnaire evaluated social and demographic factors such as age, sex, place of residence, nationality, and educational level; the second part evaluated smoking and coffee-drinking habits. Furthermore, frequencies and percentages represented categorical variables. A continuous variable was converted to a categorical variable. The relationship between different variables is tested using the Chi-square test. The total number of respondents who completed the questionnaire was 1225, with only 422 having H. pylori entries in this study. There were 290 (68.7%) men and only 132 (31.3%) women among them; the majority were young adults (18-20 years old). More than half of the participants (53%) never smoked, 23% were active smokers, and 23% were former smokers. Around 27.1% smoke five cigarettes a day and 12.6% smoke five to 15 cigarettes a day. Three-hundred(71.1%) of the participants drank coffee. 23.9% indicated that they did not drink coffee. Of those who take coffee, more than half (51.7%) take fewer than three cups daily and 25.6% take approximately three to five cups of coffee per day. Our findings indicate a link between coffee consumption and UGISs (p = 0.00), while smoking did not have a significant relationship with UGISs (p = 0.06). Our research showed that drinking coffee was related to UGISs, but smoking was not found in people with active H. pylori infection. In smokers, UGISs increased substantially, but not significantly. We need real-world research to identify the association between coffee consumption and UGISs. In addition, we need to educate people at risk for UGISs to reduce coffee, smoking, and other risk factors.

Caffeine causes cell cycle arrest at G0/G1 and increases of ubiquitinated proteins, ATP and mitochondrial membrane potential in renal cells

Caffeine is a well-known purine alkaloid commonly found in coffee. Several lines of previous and recent evidence have shown that habitual coffee drinking is associated with lower risks for chronic kidney disease (CKD) and nephrolithiasis. However, cellular and molecular mechanisms underlying its renoprotective effects remain largely unknown due to a lack of knowledge on cellular adaptive response to caffeine. This study investigated cellular adaptive response of renal tubular cells to caffeine at the protein level. Cellular proteome of MDCK cells treated with caffeine at a physiologic concentration (100 μM) for 24 h was analyzed comparing with that of untreated cells by label-free quantitative proteomics. From a total of 936 proteins identified, comparative analysis revealed significant changes in levels of 148 proteins induced by caffeine. These significantly altered proteins were involved mainly in proteasome, ribosome, tricarboxylic acid (TCA) (or Krebs) cycle, DNA replication, spliceosome, biosynthesis of amino acid, carbon metabolism, nucleocytoplasmic transport, cell cycle, cytoplasmic translation, translation initiation, and mRNA metabolic process. Functional validation by various assays confirmed that caffeine decreased cell population at G2/M, increased cell population at G0/G1, increased level of ubiquitinated proteins, increased intracellular ATP and enhanced mitochondrial membrane potential in MDCK cells. These data may help unravelling molecular mechanisms underlying the biological effects of caffeine on renal tubular cells at cellular and protein levels.

The Relationship between Smoking and Coffee Drinking Habits with Hypertension in Internal Medicine Polyclinic Patients at Malingping Hospital Banten

Background: Hypertension is a disease that is often found in the community and its prevalence continues to increase. One of the modifiable factors related to hypertension is smoking and dietary consumption. This study aimed to identify the relationships between smoking and drinking coffee with the incidence of hypertension in Internal Medicine Polyclinic patients at Malingping Hospital. Methods: The study used is cross-sectional. Data was collected by measuring blood pressure and questionnaires. A sample of 52 respondents was taken by accidental sampling and analyzed using chi-square. Results: The study showed that there was a significant relationship between smoking and hypertension P-value = 0.021 and coffee drinking habits with hypertension P value = 0.001. Conclusion: Hospitals or health workers are expected to provide counselling in the form of health education about managing smoking habits and coffee consumption in an effort to prevent hypertension in the community. Keywords: hypertension, smoking, drinking coffee

ANALISIS PERILAKU MASYARAKAT TERHADAP KEJADIAN PENYAKIT DEGENERATIF DI WILAYAH KERJA PUSKESMAS KOTA BANJARMASIN

Degenerative disease is a contributor to death from non-communicable diseases (NCD) in the last 10years which has increased from 41.7%. The latest survey in Indonesia shows PTM dominates the top 10 causes ofdeath in all age groups, with stroke, which is a complication as the number one cause of death. This studyaims to determine the relationship between length of smoking and coffee drinking habits with the incidenceof hypertension in the working area of the Banjarmasin City Health Center which was carried out in 2019.This type of research is an analytical survey with a cross-sectional research design. The subjects in this studyamounted to 57 respondents, consisting of male active smokers and consuming coffee. Data were obtainedthrough questionnaires and direct interviews as well as physical examination in the form of blood pressuremeasurements by puskesmas officers, which were then analyzed by chi-square test (α=0.05). The results ofthe statistical test showed that there was a relationship between length of smoking and the incidence ofhypertension (P=0.000), and the habit of drinking coffee with the incidence of hypertension (P=0.002).Suggestions that can be given in research are for puskesmas to further increase public knowledge abouthealth through counseling, especially about the incidence of hypertension, for health workers to optimizecounseling about the incidence of hypertension, especially for men who smoke and consume coffee.experience and add insight, and for other researchers can be used as basic data for further research.

The Relationship between Habitual Coffee Drinking and the Prevalence of Metabolic Syndrome in Taiwanese Adults: Evidence from the Taiwan Biobank Database.

Previous studies revealed inconsistent results between coffee drinking and metabolic syndrome (MetS). The aim of the study was to evaluate the relationship between habitual coffee drinking and the prevalence of MetS among men and women. We conducted a nationwide, cross-sectional study using 23,073 adults obtained from the Taiwan Biobank database (mean ± SD (range) age, 54.57 ± 0.07 (30–79) years; 8341 men and 14,731 (63.8%) women). Adults who drank more than one cup of coffee per day (n = 5118) and those who drank less than one cup per day (n = 4515) were compared with nondrinkers (n = 13,439). Multivariate logistic regression models were used to evaluate the risk of MetS between the two groups. Separate models were also estimated for sex-stratified and habitual coffee-type-stratified (black coffee (BC), coffee with creamer (CC), and coffee with milk (CM)) subgroup analyses. The MetS diagnosis was based on at least three of the five metabolic abnormalities. Coffee drinkers (≥1 cup/day) had a significantly lower prevalence of MetS than nondrinkers (AOR (95% CI): 0.80 (0.73–0.87)). Women who drank any amount of coffee and any type of coffee were more likely to have a significantly lower prevalence of MetS than nondrinkers. Only men who drank more than one cup of coffee per day or black coffee drinkers were more likely to have a lower prevalence of MetS. Our study results indicate that adults with habitual coffee drinking behaviors of more than one cup per day were associated with a lower prevalence of MetS. Moreover, women could benefit from habitual coffee drinking of all three coffee types, whereas men could only benefit from drinking BC.

No Association Observed between Coffee Intake and Risk of Non-Hodgkin Lymphoma among Postmenopausal Women

BackgroundSome preliminary studies indicate that components in coffee may have anticarcinogenic effects. However, the association between coffee-drinking habits and the risk of non-Hodgkin lymphoma (NHL) remain controversial. ObjectiveTo examine the relationship between coffee intake and NHL incidence in a large prospective study of postmenopausal US women. Design and participants/settingThe participants included 74,935 women from the Women’s Health Initiative Observational Study who were recruited from 1993 through 1998. Information about coffee-drinking habits was collected at baseline via self-administered questionnaires. Main outcome measuresNewly diagnosed NHL was validated by medical records and pathology records. Separate analyses were performed for the following three subtypes of NHL: diffuse large B-cell lymphoma (n = 244), follicular lymphoma (n = 166), and chronic lymphocytic leukemia/small lymphocytic lymphoma (n = 64). Statistical analyses performedAge-adjusted and multivariable-adjusted Cox proportional hazards models were used to determine associations of coffee intake (specifically, the total amount of coffee consumed daily, coffee types, and coffee preparation methods) with risk of NHL. ResultsA total of 851 women developed NHL during a median 18.34 years of follow-up (range = 0.01 to 24.30 years; ± 6.63 years). Overall, no associations were observed between coffee intake and risk of NHL regardless of the total amount of daily coffee intake (P value for trend = 0.90), caffeinated (P = 0.55) or decaffeinated coffee intake (P = 0.78), and filtered or unfiltered coffee intake (P = 0.91) after controlling for sociodemographic factors, lifestyle risk factors, and clinical risk factors/current medical conditions. No significant associations were observed between coffee intake with specific subtypes of NHL. A statistically significant interaction was found between alcohol intake, coffee intake, and incident NHL (P value for interaction = 0.02) based on the adjusted analysis. Specifically, among women who frequently consumed alcohol (> 7 drinks/week), those who had moderate coffee intake (2 to 3 c coffee/day) had a significantly reduced risk of developing NHL (hazard ratio 0.61, 95% CI 0.36 to 0.98), compared with those who did not drink coffee. ConclusionsThe findings from this study do not support an association between coffee consumption and NHL risk, irrespective of the total amount of daily coffee intake, coffee types, or coffee preparation methods.

Hydrogen-rich water and caffeine for alertness and brain metabolism in sleep-deprived habitual coffee drinkers.

The main aim of this randomized‐controlled cross‐over interventional trial was to assess the acute effects of taking a single dose of hydrogen‐rich water (HRW), and compare it with caffeine, HRW plus caffeine, and control water, for alertness, brain metabolism, brain and oxygen saturation, and self‐reported adverse events in healthy men and women who were habitual coffee drinkers and were sleep‐deprived for 24 hr. Sixteen apparently healthy young adults (8 men and 8 women; age 24.0 ± 3.5 years) were allocated in a cross‐over design to receive a single‐dose drink of HRW (8 ppm), caffeine (50 mg), HRW plus caffeine, or control drink (tap water) in the morning after 24‐hr sleep deprivation and 12‐hr fasting. The primary and secondary outcomes were assessed at baseline (pre‐intervention) and 15‐min follow‐up. Significantly less time was needed to complete trail‐making test after both HRW and HRW plus caffeine compared with the control drink (p < .05). The number of errors in the symbol digit modalities test was significantly lower after drinking HRW or caffeine than control drink (p < .05). Both HRW and caffeine significantly increased the choline‐to‐creatine ratio in several brain regions (frontal white and gray matter), while HRW and the combination intervention also affected brain metabolism in the paracentral brain. No participants reported any side effects from any intervention. The attention enhancement driven by HRW appears along with changes in brain metabolism. Being generally recognized as a safe intervention, hydrogen could be thus recommended as a novel intervention that upholds attention in stressed conditions, with its metabolic footprint likely different from caffeine.

Habitual coffee drinkers display a distinct pattern of brain functional connectivity

Coffee is the most widely consumed source of caffeine worldwide, partly due to the psychoactive effects of this methylxanthine. Interestingly, the effects of its chronic consumption on the brain’s intrinsic functional networks are still largely unknown. This study provides the first extended characterization of the effects of chronic coffee consumption on human brain networks. Subjects were recruited and divided into two groups: habitual coffee drinkers (CD) and non-coffee drinkers (NCD). Resting-state functional magnetic resonance imaging (fMRI) was acquired in these volunteers who were also assessed regarding stress, anxiety, and depression scores. In the neuroimaging evaluation, the CD group showed decreased functional connectivity in the somatosensory and limbic networks during resting state as assessed with independent component analysis. The CD group also showed decreased functional connectivity in a network comprising subcortical and posterior brain regions associated with somatosensory, motor, and emotional processing as assessed with network-based statistics; moreover, CD displayed longer lifetime of a functional network involving subcortical regions, the visual network and the cerebellum. Importantly, all these differences were dependent on the frequency of caffeine consumption, and were reproduced after NCD drank coffee. CD showed higher stress levels than NCD, and although no other group effects were observed in this psychological assessment, increased frequency of caffeine consumption was also associated with increased anxiety in males. In conclusion, higher consumption of coffee and caffeinated products has an impact in brain functional connectivity at rest with implications in emotionality, alertness, and readiness to action.

Anticancer activity of Lampung Robusta Coffee extract fractions

Coffee is a popular beverage that is consumed worldwide. Habitual coffee drinking is associated with cancer risk reduction including pre- and post-menopausal breast cancer. The invert correlation between coffee intake and breast cancer risk is weak, therefore coffee is potential for functional beverage. Coffee should be taken for several times per day to get its benefit. Compounds which give the cancer reduction effect are still debatable, they can be compounds with antioxidant or non-antioxidant properties. Robusta coffee from Lampung has a special meaning for Indonesian coffee lovers due to its strong aroma and flavour with affordable price. In order to observe the anticancer activity of Lampung Robusta coffee, its aqueous methanol extract was passed through a chromatography column containing polyamide with aqueous methanol as the eluent with a gradient of 10%. A total of 54 fractions were obtained and labelled as A to K fractions. Chlorogenic acid was detected in fractions A and B. Meanwhile, it was not detected in other fractions including G fraction. This G fraction, however, was the only one to show cytotoxicity against MCF-7 breast cancer cell line. This result suggests that this fraction contains potential compounds with anticancer activity.

Coffee C21 and protection of DNA from strand breaks: evaluation of a health claim pursuant to Article 13(5) of Regulation (EC) No1924/2006.

Following an application from Tchibo GmbH submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Germany, the EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA) was asked to deliver an opinion on the scientific substantiation of a health claim related to Coffee C21 and protection of DNA from strand breaks. The scope of the application was proposed to fall under a health claim based on newly developed scientific evidence. The food proposed by the applicant as the subject of the health claim is Coffee C21. The Panel considers that Coffee C21, a coffee standardised by its concentration of caffeoylquinic acids (CQA), trigonelline and N‐methylpyridinium (NMP), is sufficiently characterised in relation to the claimed effect. The Panel considers that the claimed effect, protection of DNA from strand breaks, is a beneficial physiological effect. Out of the two human intervention studies from which conclusion could be drawn, one study provides some evidence that daily consumption of Coffee C21 (750 mL/day) for 4 weeks decreases DNA strand breaks in habitual coffee drinkers after coffee withdrawal over the previous four weeks. However, the results of this study were not replicated in another study conducted under similar conditions in the same study centre. No studies performed in a different setting, from which conclusions could be drawn, were available. No evidence has been provided for a mechanism by which coffee (including Coffee C21) would reduce DNA damage in human cells by reducing DNA strand breaks. The Panel concludes that a cause and effect relationship has not been established between the consumption of Coffee C21 and protection of DNA from strand breaks.

Caffeine Uptake into the Vitreous after Peroral Coffee Consumption

Introduction: Caffeine and its metabolites have antioxidant activity, scavenging reactive oxygen species. The aim of our study was to measure caffeine concentrations in vitreous samples after peroral caffeine intake. Methods: This prospective study included patients scheduled for 23-G pars plana vitrectomy with membrane peeling due to epiretinal membranes. The study was performed in two parts: in the first part, patients were recruited into three different groups: group A consisted of habitual coffee drinkers who agreed to drink coffee containing 180 mg caffeine 1 h before surgery (n = 10), group B consisted of habitual coffee drinkers who were not offered coffee before surgery (n = 5), and group C consisted of non-habitual coffee drinkers, forming the control group (n = 5). In the second part (group D) patients (habitual coffee drinkers) agreed to give additional blood serum samples for measurement of caffeine concentration. Harvested samples of vitreous (groups A–D), epiretinal membranes (groups A–C), and blood serum samples (group D) were examined for concentrations of caffeine with gas chromatography-mass spectrometry. Results: Samples of 40 eyes of 40 patients were harvested. The concentrations of caffeine in the vitreous samples were 1,998 ± 967 ng/mL in group A and 1,108 ± 874 ng/mL in group B. In group C, caffeine concentrations were below 176 ng/mL in all vitreous samples. Both groups A and B had significantly higher concentrations of caffeine in the vitreous samples than group C (p < 0.002, p < 0.01, Mann-Whitney U test). Caffeine concentrations in epiretinal membranes were below the limits of detection. Correlation of caffeine concentrations between blood serum samples and vitreous samples in group D was high, with significantly higher caffeine concentrations in the blood serum. Conclusion: Coffee consumption leads to significant caffeine levels in the vitreous compared to patients in the control group, and caffeine concentrations in the vitreous showed a high correlation to blood serum concentrations of caffeine after peroral coffee consumption.

Habitual Coffee Drinkers May Present Conditioned Responses from Coffee-Cue

The present study was conducted to investigate whether the smell and sight of coffee (without tasting it) induced a conditioned response, and to compare the effect of the smell and sight of coffee in high- and low-consumption coffee drinkers. If classical conditioning caused the reduced reaction time effect, it was predicted that the degree of effect of caffeine-related stimuli would depend on the degree of experience of the pairing of conditioned stimulus (CS) and unconditioned stimulus (US). Sixty-six undergraduate students were randomly allocated to either a coffee or water presentation group and completed a simple reaction time task. Linear mixed modeling revealed that years of coffee consumption predicted reaction time when participants were presented coffee. The result coincided with the prediction that the greater the degree of experience of the pairing of CS and US, the greater the conditioned response would be. In conclusion, the present study showed that the smell and sight of coffee (without tasting it) induced a conditioned response and the direction was that of a caffeine-like effect. At least in behavioral effect, different types of caffeine CS uniformly induce a caffeine-like effect. Future studies should investigate the effect of the smell “or” sight of coffee to determine the relationship between CS type and direction of response.

FAKTOR–FAKTOR YANG BERHUBUNGAN DENGAN KEJADIAN GASTRITIS PADA PASIEN YANG BEROBAT DI PUSKESMAS

Gastritis is a health problem that is still very common in the community. gastritis is an inflammation or bleeding in the gastric mucosa caused by irritation, infection, and irregularities in eating patterns. Based on observations at the Lak-Lak Public Health Center in Ketambe Sub-District, Southeast Aceh Regency, the number of gastritis sufferers in January to March 2019 totaled 128 people and to date the factors associated with the incidence of gastritis in this region are not yet clearly known. This study aims to determine the factors associated with gastritis in patients seeking treatment at the Lak-Lak Public Health Center in Ketambe District, Southeast Aceh Regency in 2019. This study used a cross sectional approach. The results of the study using the Chi-square test based on factors of eating habits and coffee drinking habits are factors associated with gastritis because of the p-value of ≤ 0.05. While smoking habits, alcohol consumption habits and stress are factors that are not related to gastritis due to the p-value ≥ 0.05.&#x0D; &#x0D; Keywords : Gastritis, Eating Habits, Coffee Drinking habits, Smoking Habits, Alcohol Consumption Habits and Stress.