Abstract Background The emergence of the COVID-19 pandemic in 2019 imposed significant shifts in global health, particularly in understanding and addressing anxiety disorders precipitated by pandemic-related stressors. The current study aimed to adapt and validate the COVID-19 Anxiety Questionnaire (C-19 A) for Arabic-speaking populations (C-19 A-A), assessing its structural integrity, concurrent validity, factorial validity, and reliability. Methods The data of 571 individuals, including age range, educational history, gender, and marital status, were collected through online platforms. The translation process of C-19 A-A underwent rigorous verification by linguistic and subject matter experts. Several advanced statistical procedures were used to examine the C-19 A-A’s reliability, validity, and measurement invariances across demographic subsets. Results The results indicated robust internal consistency, with a Cronbach’s alpha coefficient of 0.889, confirming the questionnaire’s reliability. Concurrent validity showed significant and positive correlations between C-19-A and FCV-19S (r = 0.77, p < 0.000). EFA revealed a dominant factor explaining 60.4% of the variance, bolstering the questionnaire’s factorial validity. CFA further validated the model’s adequacy across different demographic groups. Specifically, demographic factors such as age, gender, and educational level showed distinct patterns of COVID-19-related anxiety. Conclusion The validated Arabic version of the C-19 A-A represents a pivotal tool for assessing pandemic-induced anxiety within Arabic-speaking communities. Its deployment enables targeted identification of vulnerable individuals, facilitating tailored health interventions and informing clinical strategies. This research underscores the pivotal role of culturally attuned assessment instruments in shaping effective public health responses and therapeutic interventions aimed at mitigating the psychological sequelae of global health crises.