Cod Liver Research Articles

Determination of vitamin D3 content in cod liver oil using column-switching technique.

To develop a two-dimensional liquid chromatography method to determine the content of vitamin D3 in cod liver oil preparations. The samples were prepared by saponification and extraction, and the content of vitamin D3 was determined by two-dimensional liquid chromatography with dual-pump-single-valve switching dual detectors. The chromatographic column, capture device, and detection wavelength were optimized; the linearity, system suitability, recovery rate, repeatability and sample stability of the method were investigated, and further validated in actual sample determination. A column switching two-dimensional chromatography method was developed. In the first dimensional chromatography, an Agilent PoroShell SB-C8 (50 mm×4.6 mm, 2.7 μm) column was used, and acetonitrile-water was used as mobile phase and gradiently eluted at a flow rate of 1.0 mL/min, the detection wavelength was 264 nm. An Agilent Zorbax SB-C18 (150 mm×3.0 mm,1.8 μm) column was used in the second dimensional chromatography, and acetonitrile was used as mobile phase at a flow rate of 0.42 mL/min, the detection wavelength was 264 nm. In determining content of Vitamin D3, there was a good linear relationship in the concentration range and the system suitability, recovery rate, repeatability and sample stability all met the verification requirements. The developed two-dimensional liquid chromatography method in the study is accurate, reproducible and simple, it can simultaneously separate pre-vitamin D3, trans-vitamin D3, vitamin D3, and tachysterol3, the baseline separation was achieved, indicating that it is worth promoting for application.

Utilizing foam-structured hydroxypropyl methylcellulose oleogels with curcumin for deep-fried chicken nuggets in carnauba wax-canola oil oleogel

In this study, foam-structured hydroxypropyl methylcellulose was used to structure cod liver oil into oleogel. Different concentrations of curcumin (0, 1.1, 2.2, 3.3, and 4.4 mg/100g of chicken nuggets) were added to the oleogels and incorporated into the chicken nuggets. Subsequently, samples were deep-fried in an oleogel frying medium prepared with carnauba wax and canola oil (10g/100g). Our results revealed that the addition of 4.4 mg/100g of curcumin led to the highest fat uptake reduction and moisture retention compared to the control and 1.1 mg/100g of curcumin (P < 0.05). Additionally, the addition of 4.4 mg/100g of curcumin exhibited consistently higher oxidative stability, as indicated by thiobarbituric acid reactive substances (TBARS), during the 30-day storage period at −18 °C compared to the control (P < 0.05). Notably, treated samples did not show any alteration in ash and protein contents, color, texture, pH values, sensory attributes, or microscopic structures of the fried chicken products. Furthermore, fatty acid analysis revealed that the samples containing 4.4 mg/100g of curcumin had significantly lower levels of saturated fatty acids and higher levels of monounsaturated and polyunsaturated fatty acids than the control (P < 0.05). Overall, the study demonstrates that adding curcumin in a carrier setting to deep-fried chicken products can improve the products by reducing fat uptake and extending oxidative stability.

Prophylactic Effect of Cod Liver Oil on Stone Formation in Rat Kidney: A Novel Approach

Prophylactic Effect of Cod Liver Oil on Stone Formation in Rat Kidney: A Novel Approach

UV-induced photocleavage of unsaturated fatty esters in cod liver oil: Impact on iodine value and stability

UV-induced photocleavage of unsaturated fatty esters in cod liver oil: Impact on iodine value and stability

Pickering emulsion gel of polyunsaturated fatty acid-rich oils stabilized by zein-tannic acid green nanoparticles for storage and oxidation stability enhancement

HypothesisPoor storage stability and oxidative deterioration are the common drawbacks of edible oils rich in polyunsaturated fatty acids (PUFAs). We hypothesized that the natural zein/tannic acid self-assembly nanoparticles (ZT NPs) could be employed as stabilizers to anchor at the oil–water interface, thus constructing Pickering emulsion gel (PKEG) system for three types of PUFA-rich oils, soybean oil (SO), fish oil (FO) and cod liver oil (CLO), to improve the storage and oxidation stability. ExperimentsZT NPs were prepared by the anti-solvent coprecipitation method, and the three-phase contact angle, FT-IR, and XRD were mainly characterized. To observe the shell-core structure and oil–water interface details of SO/FO/CLO PKEGs by confocal laser scanning microscope and cryo-scanning electron microscope. Accelerated oxidation of FO was performed to assess the protective effect of PKEG on lipids. FindingsThe SO, FO, and CLO PKEGs stabilized by 2 % ZT NPs, with oil fraction (φ = 0.5–0.6), were obtained. PKEGs show high viscoelasticity, clear shell-core structure spatial network structure, and ideal storage stability. Under accelerated oxidation, the degree of oxidative rancidity of FO PKEG was obviously lower than that of free FO. Overall, this work opens up new possibilities for using natural PKEG to prevent oxidative deterioration and prolong the shelf-life of PUFA-rich oils.

(091) EXPOSURE OF THE VESTIBULE TO A POSSIBLE ALLERGIC TRIGGER AT A VERY YOUNG AGE: LIFELONG NEUROPROLIFERATIVE VESTIBULODYNIA

Abstract Introduction Vestibular fibroblasts from patients with neuroproliferative vestibulodynia (NPV) versus age-matched controls produced higher proinflammatory cytokine levels of various interleukins when provoked with candida albicans in vitro. In genetically susceptible patients, this enhanced cytokine synthesis is hypothesized, in part, to lead to neuroproliferation in vestibular tissue resulting in allodynia/hyperalgesia. Patients with acquired NPV, who initially experienced pain-free penetration in their early adult reproductive life, may experience persistent severe vestibular burning following an allergic reaction to a topical agent applied to the non-keratinized vestibular epithelium. In contrast, patients with lifelong NPV present with the classic history of inability to insert a tampon at menarche (mean age 12.4 years). Patients with lifelong NPV may have unknowingly been exposed to an allergen early in their childhood, potentially even triggered from ointments used in infancy to protect from diaper rash, resulting in local vestibular mast cell accumulation in genetically susceptible patients. Agents commonly used to prevent are zinc oxide 40%, (Desitin) with inactive ingredients petrolatum, cod liver oil, lanolin, talc, glycerin, sorbitan sesquioleate, beeswax, tocopheryl acetate and fragrance and A+D ointment that includes petroleum jelly, lanolin, lavender oil, light liquid paraffin, vitamin A and vitamin D. It is possible an allergic reaction could result from any of these ingredients when applied to the non-keratinized vestibular epithelium. Objective We wished to better understand the possible triggering event in patients with lifelong NPV. Methods A chart review was performed of patients who presented between January 2019 and June 2023 who were clinically suspected to have lifelong NPV based on a history of inability to use tampons and inability to have pain-free penetration and histopathlogically confirmed to have NPV. Information was obtained from a parent or caregiver who regularly changed patient’s diapers as to what agents were utilized to manage diaper rash. Results Our patient cohort included 14 individuals with vulvas, mean age 25 (range 18-37) years. One patient prone to diaper rash was treated with A+D baby diaper rash ointment and Flanders buttocks ointment (zinc oxide, white petrolatum, white beeswax, Peruvian balsam). One patient had a “bad” yeast infection as a baby and received “topical antibiotics”. Five patients claimed Desitin and Vaseline were used while six patients claimed Desitin alone, two of whom cryied every time they Desitin was applied. One patient from Puerto Rico, received “a sock filled with cornstarch that dabbed the vaginal area and crevices after wiping the area clean”. Conclusions The endodermal vestibular epithelium is not protected by a keratin layer, including in babies at a very young age. In genetically susceptible babies, an allergic reaction to an active/inactive agent used to prevent or treat diaper rash could have potentially triggered mast cell accumulation in the baby’s vestibule leading to increased synthesis of cytokines and growth factors resulting in neuroproliferation consistent with lifelong NPV, evidenced by the inability to place a tampon at menarche. More research into this concern is needed. Disclosure No.

Analysis of lard in cod liver oil emulsion using FTIR spectroscopy combined principal component analysis

Cod liver oil (CLO) contains essential fatty acids and has a high price in the market. Therefore, CLO can be targeted for adulteration to increase economic profits. One of the substances used for adulteration of CLO is lard. This study aimed to analyze the spectral profile of lard and CLO in cod liver oil emulsion using Fourier transform infrared (FTIR) spectroscopy combined with principal component analysis (PCA). Lard and CLO were mixed with a range of concentrations of 0-100% used as the control. CLO emulsions were made with a composition of lard and CLO in concentrations similar to those of the control. To analyze the lard and CLO spectrum, lard and CLO were extracted from the emulsion using liquid-liquid extraction, and then the oil extracted from the emulsion was analyzed using FTIR spectroscopy and PCA. Fourier transform infrared spectrum absorption at region 1117–1098 cm-1 is specific to identifying the different spectrums of CLO and lard. The results of PCA showed that clustering between similar oil content and a combination of FTIR and PCA is effective in distinguishing the spectrum of lard and CLO in cod liver oil emulsion.

Possible Impact of Cod Liver Oil on Ovariectomy-Induced Skeletal Muscle Damage in Adult Female Albino Rats: A Histological, Histochemical, and Immunohistochemical study

Possible Impact of Cod Liver Oil on Ovariectomy-Induced Skeletal Muscle Damage in Adult Female Albino Rats: A Histological, Histochemical, and Immunohistochemical study

Hormoanl and Semen Production Response to Oral Combination of Tigernut, Soybean, Datefruit Extract and Cod Liver Oil Administration in Male Wistar Rats

Aim/objective: This study assess the effect of combined oral administration of tigernut, datefruit, soybean extract and liquid codliver oil on semen and hormonal indices such as sperm count, viability, ph., testosterone, FSH, LH, PSA etc using male wistar rats. Methods: The Tiet and Layman methods were used to determine the concerned hormones analyzed while the PSA and semen was calculated using calibrated curve method and hemocytometer Method. The animals were grouped into two phases. Hormonal (1) phase: This consist of 25 animals with group (1) serving as the control, (2) received 1000mg/kg soybean, (3) received 800mg/kg tigernut and datefruit, (4) 800mg/kg of combined extract of tigernut, datefruit, and soybean daily while group5 received a standard drug of 500mg/kg codliver oil daily. Phase (2) Semen analysis: This phase consist of 35 animals with group (1) also serving as control but group (2) receive 1400mg/kg tigernut, (3) receive 800mg/kg datefruit, (4) receive 1000mg/kg soybean, (5) receive 800mg/kg datefruit/tigernut, (6) receive 800mg/kg of tigernut, datefruit, and soybean extract each daily while group7 was administered 500mg/kg codliver oil daily for 35days. Results: The outcome of this study indicate an increase mean values for insulin, LH and testosterone among the test group compared with control. The FSH level was significantly higher in group2 (3.60m/u/ml) but decreases in group3 - group5 compared with the control (3.00m/u/ml) level. There was a significant positive correlation that exist between FSH and LH while the PSA levels are within normal range. Both active and sperm count were increased in percentage and in millions among group5 and group6 administered the combined extract of the fruits daily in comparison with control group. A significant increase level of testosterone and improved normal/active sperm count among the test group administered soybean extract and codliver oil daily was observed. Conclusion: This study is a break through on ..

Investigating the role of Vitamins in Prostate cancer risk among African Americans

Background &amp; Aims: Previous studies have shown certain vitamins and minerals are associated with a reduced risk of prostate cancer. In this study, we assess whether vitamins will have an effect on prostate cancer risk among African Americans. Methods: A case-control study was conducted in Washington, District of Columbia and Nashville, Tennessee. There were 264 participants involved in the study. The participants were divided into 3 groups: prostate cancer (n=56), elevated PSA (n=377) and control (n=163). Dietary intake was measured by using a validated food frequency questionnaire. Food items rich in vitamin D, vitamin E, selenium, and lycopene were identified. Chi square tests were performed to evaluate the association between vitamins and prostate cancer risk. Results: There is a statistically significant association between the consumption of certain food items such as beef, pork, eggs and decreased prostate cancer risk (p&lt;.05). There is also a statistically significant association between the consumption of salmon, cod liver oil, peanuts, mushrooms, orange juice and prostate cancer risk increase (p&lt;.05). When aggregated, there was no significant correlation between the consumption of vitamin D, vitamin E, lycopene, selenium and decreased prostate cancer risk. Conclusions: Results of our case-control study suggest dietary restriction of certain meats as prostate cancer risk reduction strategy cannot be suggested. Increasing annual intake of food high in vitamin D, vitamin E, selenium, and lycopene cannot be suggested as a way to decrease prostate cancer risk. Diet modification interventions should focus on dietary moderations. Center of Excellence COE/Offce of Dean, Meharry Medical College School of Medicine, Grant Number: D34HP16299. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Specific temperature-modulated crab shell-derived porous carbon as a typical recycling material for nitrofurazone electrochemical sensor

Nitrofurazone (NFZ), a nitroimidazole antibiotic, can cause serious toxicity when overused, its quantification is crucial for human health. Herein, crab shell waste is converted into porous biochar at a high temperature, which can be employed for the establishment of an electrochemical sensor for the quantitative detection of NFZ. The morphological and structural properties of the crab shell carbon at different temperatures were characterized by SEM, XRD, FT-IR and N2 adsorption-desorption analyses. The crab shell carbon prepared at 500 °C (C-CS-500) and 700 °C (C-CS-700) is calcite-based and contains micro-fibrillar similar to chitin structure, while the crab shell carbon prepared at 900 °C (C-CS-900) is lime-based and lacks the similar structure. And the synergistic influence of calcite-based composition and micro-fibrillar structure of crab shell carbon offered strong electron-transport properties and a large electrode active at a higher carbonized temperature. The electrochemical signals demonstrated that the crab shell carbon prepared at 700 °C provided enhanced sensing capability for rapid NFZ detection with a wide linear range of 0.40–80 μM, a high sensitivity of 0.55 μA μM−1 and a low detection limit of 0.11 μM (S/N = 3), as compared to the crab shell carbon prepared at 500 °C and 900 °C. In addition, the sensor can also be utilized to quantify nitrofurazone in the real drug (compound cod liver oil ointment) with satisfactory recovery.

The effect of adding cod liver oil to the diet on the productivity and blood parameters of broiler chickens

The experiment was performed to evaluate the production performance and blood parameters of broiler chicken fed on cod liver oil enriched feed. A cohort of 96 day-old chickens was allocated at random into four treatment groups, denoted as T0, T1, T2, and T3. Each treatment group consisted of three replicates. In contrast to each replication consisting of eight birds, each treatment group comprises 24 birds. Experimental birds in T1, T2, and T3 were fed rations enriched with cod liver oil at 0.5%, 1%, and 2% by weight, respectively, whereas T0 received only standard feed and was designated as the control group. The findings of this research demonstrated that the final live weight and live weight gain of the birds in group T3 which was provided with feed enriched with 2% cod liver oil was significantly greater (p

Ethyl Cellulose-Core, OSA Starch-Shell Electrosprayed Microcapsules Enhance the Oxidative Stability of Loaded Fish Oil.

The encapsulation and the oxidative stability of cod liver fish oil (CLO) within coaxial electrosprayed (ethyl cellulose/CLO) core-(octenyl succinic anhydride, OSA-modified starch) shell, and monoaxial electrosprayed ethyl cellulose/CLO microcapsules were investigated. Core-shell (H-ECLO) and monoaxial (ECLO) electrosprayed microcapsules with an average diameter of 2.8 ± 1.8 µm, and 2.2 ± 1.4 µm, respectively, were produced. Confocal microscopy confirmed not only the core-shell structure of the H-ECLO microcapsules, but also the location of the CLO in the core. However, for the ECLO microcapsules, the CLO was distributed on the microcapsules' surface, as also confirmed by Raman spectroscopy. Atomic force microscopy showed that the average surface adhesion of the H-ECLO microcapsules was significantly lower (5.41 ± 0.31 nN) than ECLO microcapsules (18.18 ± 1.07 nN), while the H-ECLO microcapsules showed a remarkably higher Young's modulus (33.84 ± 4.36 MPa) than the ECLO microcapsules (6.64 ± 0.84 MPa). Differential scanning calorimetry results confirmed that the H-ECLO microcapsules enhanced the oxidative stability of encapsulated CLO by about 15 times, in comparison to non-encapsulated oil, mainly by preventing the presence of the fish oil at the surface of the microcapsules, while ECLO microcapsules enhanced the oxidative stability of CLO about 2.9 times due to the hydrophobic interactions of the oil and ethyl cellulose. Furthermore, the finite element method was also used to evaluate the electric field strength distribution, which was substantially higher in the vicinity of the collector and lower in the proximity of the nozzle when the coaxial electrospray process was employed in comparison to the monoaxial process.

Optimization and preparation of a compound cod liver oil film former agent: an orthogonal design.

Background: Cod liver oil has anti-inflammatory properties and could help regulate recurrent aphthous stomatitis (RAS). An orthogonal experiment was used to evaluate and improve the dosage form of compound cod liver oil, which has replaced the previously used liniment preparation based on film method. Methods: An orthogonal experiment was adopted, and the appearance and film-forming time of the film coating agents were used as indicators. The optimal ratio in the preparation process for the compound cod liver oil film agent was then optimized. A method for determination of compound cod liver oil film was established using High-Performance Liquid Chromatography (HPLC). Results: The results indicate that the blank films prepared using 55mg polyvinyl alcohol (PVA) (PVA low), 45mg of PVA (PVA medium), and 10mg glycerol had the optimal performance, which was defined as PVAa. The drug-carrying film prepared from 3mL PVAa (i.e., film-forming material with the optimal proportion), 30mg dexamethasone acetate, and 30mg metronidazole had the optimal performance. The verified sample has a complete and smooth appearance, uniform thickness and color, and no evident bubbles, which meets the requirements for a film agent defined in the Chinese Pharmacopoeia, 2020 edition. HPLC was used to determine the major components: dexamethasone acetate, metronidazole, and dyclonine hydrochloride, and the optimal separation effect was obtained. The method has advantages of good specificity, good linear results, high recovery rate, and good repeatability. Conclusion: This study proposes an optimized compound cod liver oil film former agent and preparation method. The results indicate that the compound cod liver oil film former agent had good performance, reflecting the high feasibility of this research method. The detection method of compound cod liver oil film was established by HPLC. The method was feasible, and the validity and stability of the formulation and preparation technology were guaranteed. The role of the newly developed agent in patients with RAS should be investigated further.

Evaluation of protein and lipid ingredients through in vitro digestibility for tropical gar (Atractosteus tropicus) juveniles

Tropical gar (Atractosteus tropicus) is an ecologically, culturally, economically, and scientifically important species. Therefore, it is essential to know the available resources to design feeds for the culture of this species. This study evaluated the in vitro digestibility of meals and oils commonly used in aquaculture, using crude extract of the stomach and intestines of A. tropicus juveniles. We used the pH stat titration system to determine the degree of hydrolysis (DH%), the concentration of amino acids released (mg g of meal-1), and the speed of hydrolysis (SH%) of various animal and vegetable ingredients. The relative digestibility of each protein ingredient was calculated using bovine hemoglobin for acid digestion and Hammersten bovine casein for the alkaline phase as reference ingredients (100% digestibility). For lipid ingredients, fish oil was used as a reference. The highest relative digestibility for protein ingredients was observed with fish, crustaceans, beef, poultry meat and offal, and beef blood meals. The best lipid SH was observed with soybean lecithin and cod liver, soybean, olive, maize, and canola oils. It is possible to use a wide variety of terrestrial animal proteins and vegetable oils for manufacturing a diet for A. tropicus rearing.

STUDY OF COMMUNAL BASIUNI IMRAN ISLAMIC BOARDING SCHOOL WASTEWATER TREATMENT PLANT (WWTP) IN SAMBAS REGENCY

Islamic Boarding School has a treatment capacity of 33 m3 and an area of 24 m2. Based on the results of initial wastewater quality tests on communal WWTP, the TSS, COD, and BOD parameters are above the quality standard threshold based on the Wastewater Quality Standard of the Minister of Environment Regulation Number 68 of 2016. This evaluation aims to determine the processing efficiency of communal WWTP and evaluate the processing unit in communal WWTP as well as alternative improvements that effectively reduce the parameters of pH, TSS, BOD, COD, and fatty oils. This research method is descriptive analytics, which analyses problems from data collected descriptively to find solutions to problems. The results of this evaluation show that the TSS, COD, and BOD parameters are above the quality standard threshold. The pH parameter increased by 3.6%, removal efficiency for BOD by 76.4%, COD by 71.9%, TSS by 84.3%, and oil grease by 91.9%. Alternative WWTP improvements are carried out by redesigning the Communal WWTP, which consists of calculating the equalization unit, precipitator I, anaerobic filter, Precipitator II, disinfection, and sludge thickener, Sludge digester, sludge drying bed and analysis of the Budget Plan Cost (RAB). The communal WWTP redesign requires a land area of 52.62 m2 with a processing capacity of 56.8 m3 and a cost budget plan of Rp.157,224,000.00. The redesign results of this evaluation can reduce environmental impacts due to domestic wastewater.

Control of cod-liver oil composition with laser scattering imaging combined with machine learning procedures: The cases of adulteration and oxidation

Chemical changes in cod-liver oil produced by oxidation and adulterations with other oils were modelled using RGB-laser scattering imaging. Two types of composition-altered cod-liver oil were: oxidised oil at three different temperatures (4, 20, and 40 °C) and cod-liver oil adultered with wheat-germ, soybeana, sesame and corn. Both types of altered oils and control samples were analysed by the imaging technique and chemical measurements were also carried out to know the oxidation status. The capacity of the technique for detecting pure cod-liver oil was tested by applying multivariate regression and discriminant procedures based on PLS using the information captured from each type of laser (650 nm, 550 nm and 450 nm). The results showed the capacity of the technique to capture the variability provided by the cod-liver oil against the other vegetable oils used as adulterants. It was discriminate from all vegetable oils and all them were detected when were added as adulterants. Changes in the oxidation status were also modelled by predicting the oxidation parameters with R2 > 0.90, independently of the temperature of storage. Those capacities made it possible to discriminate pure cod-liver from all tested adulterated-oxidised samples performing a common model. The prediction capacity was synergic when models were performed including the three lasers, as opposed to the results obtained with singly-laser models.

Assessment of the quality of canned cod liver

The task of this work is to analyze the quality indicators of canned food called Cod liver in Murmansk style. To achieve this goal, methods of organoleptic and physicochemical analysis were applied. In the course of the study we studied the change in the quality of canned food made from the liver of raw Atlantic cod (Gadus morhua, Linnaeus, 1758), which was caught in the fall-winter period. For this purpose, the actual content of fats, proteins, carbohydrates, vitamins A and B2, the acid number and the calculation of the product energy value were determined.

Stability of shark (Centrophorus sp.) liver oil rich in squalene as an immunostimulant

Shark liver oil has been widely produced in Indonesia; however, many shark liver oil products still do not meet the established standards. This research involves the addition of antioxidants to stabilize the oil as an effort to extend its shelf life. The objectives of this study were to determine the immunostimulant activity, assessed the synergistic effect of vitamin E in shark liver oil rich in squalene, and identified the optimum concentration of the antioxidant vitamin E in maintaining its stability. Immunostimulant activity was tested by observing spleenocyte proliferation, phagocytic index, and capacity in the peritoneal cavity cells of mice. The stability of the oil was examined by an accelerated method using an oven, what was called the schaal oven test at 40 °C. The research results indicate that shark liver oil possesses immunostimulant effects; however, with an increasing dosage of fish oil, there is no significant improvement. The presence of vitamin E in cod liver oil exhibited a synergistic effect on spleenocyte proliferation for all dosages, while the phagocytic index and capacity at a dosage of 100 μg/mL showed synergy. However, at a dosage of 50 μg/mL, the phagocytic index and capacity did not exhibit synergy with the immunostimulant. The optimal concentration of the antioxidant vitamin E in maintaining oil stability was found to be 100 ppm.

ASUPAN VITAMIN D YANG KURANG BERISIKO MENINGKATKAN FREKUENSI KEJADIAN ALERGI SECARA UMUM PADA PEREMPUAN DEWASA DI KOTA BOGOR

Vitamin D deficiency is not only caused by disease, but also by insufficient intake. Insufficient vitamin D levels are associated with an increased incidence of allergies. This study aimed to determine the relationship between the source of vitamin D intake and the frequency of allergies in adult women in Bogor City. This research used a cross-sectional design involving 395 women aged 19-49 years and domiciled in Bogor City from August-November 2023. The sampling technique used was purposive sampling based on inclusion criteria. Data collection was carried out by filling out a questionnaire. Bivariate data were analyzed using chi-square. The results of the study show that consumption of vitamin D has a significant effect on the incidence of allergies, where people who consume vitamin D are less at risk of developing allergies (allergic and not allergic) (0.021) and is supported by the statement that the frequency of administration of vitamin D apparently influences the risk of not experiencing allergies ( 0.033). The results showed that intake of other sources of vitamin D: fish (p=0.359), cod liver oil supplements (p=0.106), milk enriched with vitamin D (p=0.844), dairy products enriched with vitamin D (p=0.503) , is not significantly related to the frequency of allergic events. The conclusion is regular consumption of vitamin D supplements can help reduce the frequency of allergies. Further research on the effects of immunomodulation and allergy prevention with experimental approaches is needed. Future research suggestions should investigate the optimal dose of vitamin D supplements for allergy prevention.