This study investigated the physicochemical properties, sugar profiles, microbiological quality and sensory attributes of the honey produced by an Afrotropical stingless bee, Axestotrigona ferruginea, collected from two locations in Tanzania; Kibiti, representing the coastal mangrove ecosystem, and Siha, representing the northern highland’s Afromontane region. Honey samples from the two locations showed significant variations in moisture content, pH, free acidity, color, 5-hydroxymethylfurfural, total yeast and mold counts, and sensory properties (p < 0.05). However, there was no difference in the sugar profiles of the studied honey samples (p > 0.05). Overall, all honey samples met the East African quality standards for stingless bee honey, except for reducing sugars content. Additionally, all honey samples had acceptable microbial counts, indicating good quality. These findings provide valuable insights into the quality and sensory diversity of stingless bee honey, which can positively influence its acceptability and marketability.
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