Coagulation Technique Research Articles

Methods of Controlling Postoperative Complications after Lymphadenectomy in Patients with Breast Cancer

Introduction. Reducing the aggressiveness of surgical tactics is currently considered a promising trend in medicine that is widely supported due to its better safety profile. However, a large percentage of patients need more intervention. In the treatment of breast cancer, the main difficulties in postoperative management are associated with lymphatic surgery. Aim. To perform a comparative analysis of techniques for intraoperative control of lymphorrhea in patients during lymph node dissection for breast cancer in Yaroslavl Regional Oncologic Hospital. Materials and methods. Case histories of 660 patients after axillary subclavian subscapular lymphadenectomy were analyzed. In the postoperative period, patients revealed prolonged lymphorrhea. Magnifying techniques (binocular lenses, microscope) were used during the interventions to detect and isolate lymphatic vessels in 108 patients. A double coagulation technique was used to ligate the lymphatic vessels. Sufficient coagulation surface was achieved by forming suture lines of an angle greater than 90°. When performing a mastectomy, the technique was supplemented by circular bandaging of the chest. Results and discussion. Analysis of case histories showed that lymphadenectomy proved to be the main significant factor influencing lymphorrhea. Sparing techniques reduced the risk of postmastectomy syndrome. Comparing the study group with the control group revealed a statistically significant reduction in the severity of lymphorrhea without an increase in the risk of other complications. Conclusion. The combination of these techniques demonstrated statistically significant advantages in controlling postoperative complications. The techniques are easy to perform, therefore, they can be introduced into practice with minimal costs. The economic benefits are associated with the absence of additional costs for surgery, as well as with a reduction in the duration of hospital stay for patients. All this significantly increases patient satisfaction with the treatment performed.

Reuse and Recovery of Water from Industrial Textile Dyeing Effluent Using High-Performance Electrodes Continuous Flow Electrocoagulation Reactor

The dye effluents released from the textile and printing industries contain strong colorants, inorganic salts, and other toxic compounds. The conventional coagulation technique of dye effluent treatment is plagued with issues of low removal rate of color, generation of large quantities of sludge, and toxic end-products. Recently electrocoagulation technique gained immense attention due to its high efficiency. This technique involves the dissolution of the sacrificial anodes to provide an active metal hydroxide as a strong coagulant that destabilizes the pollutants and removes them by precipitation or flocculation. This study is about the efficiency of the electrocoagulation process using titanium coated - aluminum and mild steel electrodes to treat industrial dye wastewater. Effects of parameters such as current density & initial dye concentration were investigated. It was observed that, for the same current density, electrode consumption was higher with TiO2/Al electrode than with mild steel electrode, resulting in more color removal efficiency (CRE) using TiO2/Al electrode. The settling rate of the flocs was higher in the rector having TiO2/Al electrode at the 100 mL with current density (2.5 mL.min-1 to 5.3 mL.min-1), while in the reactor with mild steel electrode, the settling rate was very less. The results showed that dye removal was 95.11% and 92.1% for mild steel and titanium-coated electrodes, respectively. It was observed that 50 % of Aluminum was removed from the treated effluent after the final stage of filtration. Based on the multicriteria analysis to identify the optimum operational parameters to be applied at the field level, it was observed that maximum CRE may be obtained with TiO2/Al electrode and the applied current of 1 Amps with a flow rate of 100 mL.min-1. It can be concluded that electrocoagulation is a highly efficient and the fastest method to treat dye effluents from industries.

Development of a limestone-based ballasted material for enhanced co-removal of turbidity and fluoride from coal mine water: An efficacy study

The rapid progression of coal mining technology in recent years has resulted to the discharge of mine water with excessively high levels of turbidity and fluoride, presenting a complex contamination issue. This study develop an innovative limestone-based ballasted material, integrating it with ballasted flocculation technology. The synergistic effects of surface flocculation and adsorption have enabled the simultaneous and efficient removal of high concentrations of turbidity and fluoride. The influence of various parameters, including the dosage of the coagulant, flocculant, ballasted material, and the stability under long-term continuous operation, on the performance of this new technology was investigated. Findings indicate that an increase in the dosage of the coagulant, polyaluminum chloride (PAC), positively affects the removal of turbidity and fluoride ions, with an optimal dosage of 60 mg/L. The addition of polyacrylamide (PAM) showed that both conventional and ballasted coagulation techniques exhibit an initial increase followed by a decrease in turbidity removal efficiency, and an ascending trend in fluoride removal efficiency, with an optimal PAM dosage of 1.0 mg/L. The dosage of the new ballasted material was determined based on the initial water turbidity and fluoride concentrations, an increase in its dosage led to a progressive reduction in effluent turbidity and a corresponding increase in fluoride removal. A 30-day continuous operation confirmed the stability of the new process, with a treatment cost of approximately 2.67 $/t. The pseudo-first-order kinetic model and the Langmuir adsorption isotherm equation provided satisfactory fits to the treatment data, suggesting that both conventional and ballasted coagulation involve a combination of surface adsorption and intraparticle diffusion mechanisms for the removal of turbidity and fluoride.

Linear and nonlinear regression modelling of industrial dye adsorption using nanocellulose@chitosan nanocomposite beads

Nanocellulose@chitosan (nc@ch) composite beads were prepared via coagulation technique for the elimination of malachite green dye from aqueous solution. As malachite green dye is highly used in textile industries for dyeing purpose which after usage shows fatal effects to the ecosystems and human beings also. In this study the formulated nanocellulose@chitosan composite beads were characterized by Particle size analysis (PSA), Field emission scanning electron microscopy (FESEM), Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis were done to evaluate nanoparticles size distribution, morphological behaviour, functional group entities and degree of crystallinity of prepared beads. The nanocomposite beads adsorption performance was investigated for malachite green (MG) dye and BET analysis were also recorded to know about porous behaviour of the nanocomposite beads. Maximum removal of malachite green (MG) dye was found to be 72.0 mg/g for 100 ppm initial dye concentration. For accurate observations linear and non-linear modelling was done to know about the best-fitted adsorption model during the removal mechanism of dye molecules, on evaluating it has been observed that Langmuir isotherm and Freundlich isotherm show best-fitted observation in the case of linear and non-linear isotherm respectively (R2 = 0.96 & R2 = 0.957). In the case of kinetic linear models, the data was well fitted with pseudo-second-order showing chemosorption mechanism (R2 = 0.999), and in the case of non-linear kinetic model pseudo first order showed good fit showing physisorption mechanism during adsorption (R2 = 0.999). The thermodynamic study showed positive values for ΔH° and ΔS° throughout the adsorption process respectively, implying an endothermic behaviour. In view of cost effectiveness, desorption or regeneration study was done and it was showed that after the 5th cycle, the removal tendency had decreased from 48 to 38 % for 20–100 ppm dye solution accordingly. Thus, nanocomposite beads prepared by the coagulation method seem to be a suitable candidate for dye removal from synthetic wastewater and may have potential to be used in small scale textile industries for real wastewater treatment.

Enhanced removal of polyethylene microplastics from water through polymeric ferric sulfate with laminarin

Coagulation has been demonstrated as an effective method for eliminating microplastics from water. However, based on the existing literature, the use of a single coagulant has not yet yielded satisfactory results in microplastic removal. Hence, it is crucial to investigate the potential of enhanced coagulation techniques. In this study, laminarin (LA) was used as a novel coagulant aid to investigate its enhanced coagulation performance in the removal of polyethylene (PE) microplastics using polymeric ferric sulfate (PFS). The coagulation mechanism was investigated through zeta potential, Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). The results demonstrated that the coagulation efficiency of PFS on PE microplastics could be improved by adding an appropriate amount of LA. The removal rates of PE microplastics by single PFS and PFS-LA reached 48.5% and 93.8%, respectively, when the dosages of PFS and LA were 250 mg/L and 5 mg/L. The coagulation mechanisms of PFS and PFS-LA on PE microplastics were found to be the same, with the addition of LA significantly enhancing the effects of charge neutralization, adsorption bridging, and sweep flocculation of PFS. Various experiments conducted under different pH levels, microplastic particle sizes, coexisting ions, humic acid coexistence, and real water conditions demonstrated that the PFS-LA complex coagulation system exhibited better environmental adaptability.

Penentuan Kebutuhan Poli Aluminium Klorida pada Proses Penjernihan Air di Unit Water Treatment PT. XYZ Kuala Tanjung

A Water Treatment Unit (WTU) is a station that works on the processing and conditioning of raw water to meet the desired water quality. Water purification at PT. XYZ Kuala Tanjung uses raw water sourced from surface water or rivers. The river water used still contains solid substances and organic matter that render the water unsuitable for industrial use. Both suspended and colloidal solid substances can cause turbidity. Turbidity caused by solid substances can be addressed using coagulation techniques. The purpose of this research is to determine the optimal concentration and dosage of coagulant to clarify water in the WTU. The coagulant used is polyaluminum chloride (PAC). The research method is based on experiments using jar tests and stoichiometric calculations. The determination of the optimal coagulant is measured based on turbidity parameters. Experiments are conducted with varying concentrations of PAC ranging from 25-65 ppm, and the smallest turbidity value obtained is 1.21 NTU at a PAC concentration of 65 ppm, with a PAC requirement of 202.6679 kg/day. The higher the PAC concentration, the clearer the resulting water, but the daily requirement for PAC also increases.

Microplastic removal by coagulation: a review of optimizing the reaction conditions and mechanisms

Coagulation is a widely employed technique for removing suspended particles from water and wastewater, and recently, it has gotten attention as a popular method for the removal of microplastics (MPs). Studies on coagulation-based removal of MPs are still in their infancy, and few findings are available about this treatment approach, its mechanism, and removal efficiency. Given these gaps, this study was designed to comprehensively investigate recent advances in the removal of MPs via coagulation. The influence of various experimental factors such as coagulant type, dose of the coagulant, pH of the solution, and shape of the MPs are critically reviewed. The study findings showed that optimizing environmental conditions during the coagulation process is crucial for improving the removal of MPs and reducing energy costs. The study findings showed that the coagulation efficiency of MPs depends on optimal reaction conditions, which may vary depending on the type and concentration of MPs and the characteristics of the water or wastewater being treated. Optimizing these reaction conditions is, therefore, critical to achieving maximum removal efficiency. More extensive research is required to reveal the mechanisms of coagulation in controlling floc density and removing pollutants from effluent. Consequently, the current review aims to highlight the gaps and challenges associated with coagulation techniques for the removal of MPs during wastewater treatment. Current advancements in the synthesis and chemical modification of bio-based coagulants and their coagulation performance for the removal of MPs could constitute a paradigm shift in ecosystem protection and sustainability. The use of eco-friendly coagulants and combining coagulation with other techniques are suggested to increase the efficacy and viability of this method. This review will provide significant insights for field researchers, guiding their future investigations and contributing to the advancement of knowledge.

Coconut shell char-based nanocomposites of polyamide-6: An investigation of thermal, morphological, and crystalline properties

This research was intended to investigate the thermal, morphological, and crystalline characteristics of polyamide-6@carbon char-based nanocomposites. The mesoporous nanosized carbon char was prepared in the laboratory via the carbonization method. The parameters of the coconut shell-based biocarbon char were confirmed by surface area and particle size analysis, and X-ray diffraction analysis. The coagulation-processed nanocomposites of polyamide-6 were prepared using synthesized mesoporous carbon char as filler. The obtained filler with a Brunauer–Emmett–Teller surface area of 1517 m2/g and an average pore volume of 2.51 nm was mixed with the polyamide at five different loadings. The coagulation technique was employed for the nanocomposite synthesis. The acquired coagulated nanocomposite was investigated using Fourier transform infrared analysis, thermogravimetric analysis, differential scanning calorimetric technique, and scanning electron microscopy analysis. It was evidenced from the research that loading coconut shell-based carbon char to polyamide-6 can contribute to a 10 °C rise in final degradation temperature with a 4 % loading of filler. The same filler content has contributed to a 5 °C decline in glass transition temperature. A continuous decrease in crystallinity with a rise in the concentration of biochar is indicative of the incorporation of amorphous character. So, it was determined that the thermal, morphological, and crystalline properties have been modified by synthesizing the coagulation-processed nanocomposites with mesoporous biochar of coconut shells.

The Role of Laser Photocoagulation in Treating Diabetic Macular Edema in the Era of Intravitreal Drug Administration: A Descriptive Review.

Background and Objectives: This study aimed to elucidate the role of laser photocoagulation therapy in the treatment of diabetic macular edema (DME) as an alternative to, or in conjunction with, the first-line treatment, anti-vascular endothelial growth factor (VEGF). Materials and Methods: A comprehensive literature search to identify studies that evaluated the efficacy of laser photocoagulation therapy in the management of DME was performed. The relevant findings of the efficacy of focal/grid laser therapy from data in randomized, controlled trials were synthesized, and the potential of new laser technologies, such as navigated laser systems, pattern scan lasers, and subthreshold lasers, was explored. The usefulness of multimodal imaging-guided laser therapy was also evaluated, with a focus on the potential contribution to anti-VEGF therapy. Results: Focal laser photocoagulation targeting microaneurysms remains an effective therapeutic approach to chronic refractory edema, despite the widespread use of anti-VEGF therapy. To achieve the best possible treatment outcomes, precise identification of microaneurysms is essential. This requires the use of multimodal imaging-guided, highly accurate, minimally invasive coagulation techniques. Subthreshold laser therapy can also reduce the frequency of anti-VEGF injections and minimize treatment burden. Conclusions: Further studies are needed to determine the optimal timing and settings for laser photocoagulation therapy and the potential of new laser technologies in the management of DME. Nevertheless, laser photocoagulation therapy plays an important role in the management of DME, in conjunction with anti-VEGF therapy.

A technique of transdermal laser coagulation for treating skin venous dysplasia in children

Introduction. Venous malformation is a congenital vascular disease of venous blood flow system which is met in ratio 1–2 cases per 10 000 population. The clinical picture depends on the diameter of affected vessels, their extension and on possible complications. The disease is diagnosed both at birth and later. It has a slowly progressing course. Among current techniques applied for treating skin venous malformations, the most promising one is laser transdermal coagulation with wavelength 1064 nm. Material and methods. The article describes two clinical cases of external venous malformation treated with Nd:YAG transdermal laser coagulation using Aerolase laser device, Micropulse technology, pulse duration 650 microseconds. Results. The technique of transdermal Nd:YAG laser coagulation on the Aerolase apparatus has been used in our department since February 2021 to the present. In total, for the period from 2021 to May 2023, 98 patients with venous malformations of various localization were treated with this method. In all clinical cases, a stable positive result was achieved, consisting in a decrease in the number of pathological vessels and the area of the lesion, or its complete disappearance. According to the results of treatment, no complications were observed. Conclusion. Laser coagulation of pathological venous vessels of external location is an effective and safe modality for treating children of any age.

Hemostasis in a giant intraventricular tumor using a saline-cooled radiofrequency bipolar coagulator: technical note.

Meningiomas are relatively rare in children and tend to be intraventricular and cystic, with often malignant behavior. Complete excision is associated with the most favorable outcome; moreover, the size and extent of these lesions often make complete excision in one step impossible because of the risk of intraoperative death from uncontrollable hemorrhage. A 10-year-old girl was admitted for headache in the last 3months and was found to have a giant left intraventricular lesion with a volume of 166.63 cm3, which caused hydrocephalus and significant mass effect. Very large draining veins were evident within the tumor, draining into the thalamostriates and internal cerebral veins. Cerebral angiography showed multiple feeders originating mainly from branches of the posterior left choroidal artery with distal afferents that could not be embolized. Therefore, a left parietal transcortical approach was chosen. Given the vascularity of the tumor, saline-cooled radiofrequency coagulation (Aquamantys®) was used to reduce blood loss intraoperatively. Gross total resection (GTR) was achieved with an estimated blood loss of 640mL. Pathology analysis was consistent with WHO grade 1 transitional meningioma. Postoperatively, the patient was neurologically intact, and MRI confirmed complete resection. Aquamantys® is a novel bipolar coagulation device that employs a new bipolar coagulation technique combining radiofrequency energy and saline to achieve hemostatic sealing by denaturing collagen fibers. This offers the possibility of achieving adequate hemostasis even in giant intraventricular tumors in infants to obtain GTR resection with minimal blood loss.

Spray coagulation as an alternative to argon plasma coagulation for bleeding portal hypertensive gastropathy

Spray coagulation as an alternative to argon plasma coagulation for bleeding portal hypertensive gastropathy

Development of a Computer System for Automatically Generating a Laser Photocoagulation Plan to Improve the Retinal Coagulation Quality in the Treatment of Diabetic Retinopathy

In this article, the development of a computer system for high-tech medical uses in ophthalmology is proposed. An overview of the main methods and algorithms that formed the basis of the coagulation plan planning system is presented. The system provides the formation of a more effective plan for laser coagulation in comparison with the use of existing coagulation techniques. An analysis of monopulse- and pattern-based laser coagulation techniques in the treatment of diabetic retinopathy has shown that modern treatment methods do not provide the required efficacy of medical laser coagulation procedures, as the laser energy is nonuniformly distributed across the pigment epithelium and may exert an excessive effect on parts of the retina and anatomical elements. The analysis has shown that the efficacy of retinal laser coagulation for the treatment of diabetic retinopathy is determined by the relative position of coagulates and parameters of laser exposure. In the course of the development of the computer system proposed herein, main stages of processing diagnostic data were identified. They are as follows: the allocation of the laser exposure zone, the evaluation of laser pulse parameters that would be safe for the fundus, mapping a coagulation plan in the laser exposure zone, followed by the analysis of the generated plan for predicting the therapeutic effect. In the course of the study, it was found that the developed algorithms for placing coagulates in the area of laser exposure provide a more uniform distribution of laser energy across the pigment epithelium when compared to monopulse- and pattern-based laser coagulation techniques.

Comparison of the Two Coagulation Techniques: Thrombolyzer XRM and Diagon Coag XL.

The aim of this study was to evaluate the performance characteristics of the Thrombolyzer XRM against the Diagon Coag XL during the exchange of the coagulation analyzer. The Partial Thromboplastin Time (PTT) and prothrombin time (PT) measurements were performed by using the Thrombolyzer XRM and Diagon CoagXL analyzers. The precision, accuracy, method comparison, and reference range verification studies were performed on the Thrombolyzer XRM based on Clinical Laboratory Standards Institute guidelines. The precision study was performed with normal and pathologic controls for the PT and PTT tests, all co-efficients of variation (%) were found ≤ 2.5%. In the accuracy study, both analyzers displayed less than 6.3% and 10.8% bias for PT and PTT tests, respectively. The method comparison study demonstrated good agreement for the Bland-Altman plots for PTT and PT with a bias of -2.0 and -3.3 between each analyzer, respectively. The Passing and Bablok analysis showed no significant differences for PTT and PT between each analyzer (p = 0.65 and p = 0.33, respectively). However, there was a proportional bias for PT with a slope of 1.40 (95% CI 1.2 - 1.8). The manufacturer ranges were acceptable as a result of the reference range verification study. The routine coagulation analysis can be performed on the Thrombolyzer XRM with satisfactory precision and the obtained minor differences can be eliminated in future standardization studies.

The Treatment Effect of Chemical Coagulation Process in South African Brewery Wastewater: Comparison of Polyamine and Aluminum-Chlorohydrate coagulants

An evaluation of a laboratory scale chemical coagulation using aluminium chlorohydrate (1%) and polyamine (1%) coagulants on its effectiveness in the removal of bulk inert pollutant contents such as particulate chemical oxygen demand (COD) and turbidity to obtain clean effluent discharge and most cost-effectively treated effluent using a jar test was conducted in this current study. This study aimed to find the viable inert removal coagulant between the two above-mentioned coagulants in order to achieve zero liquid effluent discharge (ZLED). The preliminary results showed that adding variable dosages of polyamine and 50% aluminium chlorohydrate combined coagulants dosages presented an improved particulate chemical oxygen demand, color, and turbidity removal efficiencies. The ascertained turbidity removal efficiency using the combined coagulation dosage of polyamine–aluminium chlorohydrate (PAC) treatment was 90.50% and 59.36% particulate chemcial oxygen demand removal, as comparable to polyamine alone with particulate chemical oxygen demand removal of 50% and turbidity of 75%. Likewise, an appreciable removal efficacy was observed as the aluminium chlorohydrate treatment alone was for particulate chemical oxygen demand and turbidity was 37% and 54%, respectively. In essence, this study emphasized the knowledge gap of the significant effect of the polymeric polyamine flocculant strength in adopting the combined coagulation dosage method to improve its coagulation efficiency and the high agglomeration on suspended solids, thereby, removing more of the unwanted inert contents from brewery wastewater. To determine zero liquid effluent discharge, this study clearly recommended an integrated treatment approach, microbial fuel cell integrated with a lab scale chemical coagulation technique for efficient non-biodegradable pollutant removal.

Efficient removal of nano- and micro- sized plastics using a starch-based coagulant in conjunction with polysilicic acid

Microplastic (MP) pollution has increasingly become an enormous global challenge due to the ubiquity and uncertain environmental performance, especially for nano- and micro- sized MPs. In this work, the performance and mechanisms in coagulation of 100 nm–5.0 μm sized polystyrene particles using an etherified starch-based coagulant (St-CTA) assisted by polysilicic acid (PSA) were systematically studied on the basis of the changes in MPs removal rates under various pH levels and in the presence of different coexisting inorganic and organic substances, zeta potentials of supernatants, and floc properties. St-CTA in conjunction with PSA had a high performance in coagulation of nano- and micro- sized MPs from water with a lower optimal dose and larger and compacter flocs. Besides, the MPs removal rate can be improved in acidic and coexisting salt conditions. The efficient performance in removal of MPs by this enhanced coagulation was owing to the synergic effect, that is, the effective aggregation of MPs through the charge neutralization of St-CTA followed by the efficient netting-bridging effect of PSA. The effectiveness of this enhanced coagulation was further confirmed by removal of two other typical nano-sized MPs, such as poly(methyl methacrylate) and poly(vinyl chloride), from different water sources including tap water, river water, and sludge supernatant from a sewage treatment plant. This work provided a novel enhanced coagulation technique that can effectively remove nano- and micro- sized MPs from water.

Compressive optic neuropathy caused by a flow-diverter-occluded-but-still-growing supraclinoid internal carotid aneurysm: illustrative case

BACKGROUNDFlow diverter stenting is an effective treatment for large proximal internal carotid artery (ICA) aneurysms. Cranial neuropathy caused by the mass effect of the aneurysm usually subsides over time. However, a new onset of compressive optic neuropathy after successful flow diverter stenting of a large proximal ICA aneurysm is seldom reported.OBSERVATIONSA 57-year-old woman had a right supraclinoid ICA aneurysm (approximately 17 mm) on magnetic resonance angiography (MRA) in a health checkup. She received intervention with the Pipeline embolization device. Six months later, she started to experience progressive hemianopia in the left half of the visual field. Nine months after stenting, MRA showed that the aneurysm was growing and causing mass effect, but digital subtraction angiography confirmed that the aneurysm was completely excluded from the circulation. She received a craniotomy for microsurgical decompression of the optic nerve and coagulation shrinkage of the aneurysm. Clipping and thrombectomy were not attempted. Her visual fields recovered gradually. Follow-up MRA showed that the aneurysm also diminished in size.LESSONSWhether the coagulation technique of the flow-diverter-occluded aneurysm alone is enough to cause satisfactory shrinkage and interaction between the flow diverter and the aneurysmal vasa vasorum/neointima formation should be further examined.

Fibrinogen Deficiency with Thrombotic Manifestations.

Fibrinogen deficiencies are very rare. Qualitative fibrinogen deficiencies (dysfibrinogenaemia and hypodysfibrinogenemia) are functional disorders that can present with both haemorrhagic symptoms and with thrombotic phenomena as unique and paradoxical manifestation. We present the case of a 77-year-old man being investigated for a partially thrombosed abdominal aortic aneurysm as well as an ischaemic stroke 20 years previously. Basic coagulation tests were normal but extended tests revealed a lengthened thrombin time (TT) combined with a significant drop in fibrinogen concentration measured with the Clauss assay and by nephelometry. After secondary fibrinogen deficiencies were ruled out, a heterozygous variant in the FGG gene was detected by next-generation sequencing, and congenital hypodysfibrinogenemia was diagnosed. Acenocumarol was initiated and no new thrombotic or haemorrhagic events had occurred after a year of follow-up.In almost 25% of cases, thrombotic events may be the only clinical manifestation of functional fibrinogen deficiencies. They are a rare cause of thrombophilia, and are probably underdiagnosed due to normal standard coagulation test results as well as a possible absence of haemorrhagic events. Consequently, a TT test (an initial ‘rule out’ test) should be requested in order to promptly identify these patients. Moreover, discrepancies in derived and Clauss fibrinogen test results should suggest a functional disorder. Finally, new coagulation techniques based on the functional characterization of clot formation, such as ROTEM or thrombin generation assay, could help characterize these entities and suggest new therapeutic approaches.LEARNING POINTSFunctional fibrinogen deficiencies can present with thrombotic manifestations only, and are a rare and probably underdiagnosed cause of thrombophilia.Thrombin time is a highly sensitive test to rule out other conditions as aPTT and PT results may be within normal ranges, especially in functional deficiencies.Discrepancies between derived and Clauss fibrinogen findings, fibrinogen protein measurements and the use of new techniques (ROTEM or thrombin generation) are important for correct approach.

Spectroscopy characterization of acid and rennet camel milk caseins using XRD, XPS, and SEM and effects on their emulsifying properties

Casein, the main protein in milk, is a viable protein source for natural emulsifiers, which are frequently utilized in food emulsions. Camel milk casein powders obtained by acid and rennet coagulation were investigated and characterized using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). These methods gave extensive information regarding crystal structure, surface composition, and secondary and microstructures of the camel caseins, respectively. Therefrom new acknowledgements on morphology and surface composition have emerged, which is necessary to better understand functional characteristics such as emulsifying behaviour (i.e., emulsion’s stability over time and the oil droplet size). The O and C atomic proportions of camel casein surfaces generated by acidification were greater than those obtained through rennet-induced coagulation, whereas the percentage of N atomic was lower, according to XPS measurements. The oxygen to carbon (O/C) ratios on the surface of camel caseins produced by the two coagulation techniques were comparable. But, the enzymatic coagulation method yielded a high (N/C) ratio. ‎The findings suggested that rennet-induced coagulation might influence the C, O, and N components on the surface of camel milk caseins. Furthermore, SEM and XRD data revealed that acid aggregation affected casein micelle’s morphology, resulting in more β-sheet and smooth structures. Rennet camel caseins, on the other hand, have a porous colloidal surface. ‎A correlation between the casein surface composition and the emulsifying stability of the casein powders was observed, whereas the droplet size and stability of emulsions were strongly improved by the rennet-induced coagulation method.

Advanced technique of catalytic ozonation-enhanced coagulation for the efficient removal of low coagulability refractory organics from secondary effluent

The novel cascaded catalytic ozonation-enhanced coagulation process (FeCeAC/O3-PAC) was developed with much success towards second effluent organic matter (EfOM) in chemical industrial wastewater. Compared with the conventional techniques, FeCeAC/O3-PAC exhibited remarkable performances in the advanced removal of EfOM. The characteristics of EfOM and interactivities of reaction process played the crucial roles. Especially, the removal rate constant of soluble microbial products (SMPs) with FeCeAC/O3-PAC exceeded 55.38% versus FeCeAC/O3. The outstanding synergistic effect was contributed to the enhanced generation of active oxygen species by FeCeAC and PAC, which increased the content of oxygen-containing functional groups of EfOM and thus facilitated the interaction between PAC and EfOM. As the result, the larger-sized flocs could be formed and separated easily. Herein, this work found a far more effective way to remove EfOM especially low-coagulability refractory organics (LCRO) in chemical sewage plant.