The Venturi flow meter exhibits relatively low pressure loss, simple design, and low manufacturing costs. This study describes flow rates measurements for supercritical pressure CO2 using the Venturi flow meter with pressure ranging 7.379–7.836 MPa and 5.84–7.272 MPa for in supercritical and gaseous regions, respectively. The flow rates of supercritical pressure CO2 were accurately measured using a Venturi flow meter with a diameter ratio of 0.6468, having large and small diameters of 87.32 mm and 56.48 mm, respectively. The convergent and divergent angles were 21 ° ± 1 ° and 15 ° ± 1 °, respectively. The averaged discharge coefficient of 0.9975 was obtained, which was independent of the pressure ratio. Additionally, the expansion factors were also calculated using the experimental results, which ranged from 0.99976 to 0.99987 and 0.99945–0.99995 for the supercritical and gaseous regions, respectively. The experimental results showed that the Venturi flow meter had uncertainties ranging from 0.1 to 2.8%.