Co-functional Monomer Research Articles

Self-polymerization silica nanoparticles based molecularly imprinted polymers for selective recognition of protein

Molecularly imprinted polymers (MIPs) are promising for precise protein separation and purification. However, challenges persist due to their large size, variable configuration, and instability during preparation. Here, a simple silicon self-assembly program was designed to synthesize MIPs without any organic reagents and acid-base catalysis, avoiding the structural damage of protein under severe conditions. In this method, employing hemoglobin (Hb) as a model protein, with tween-20 in emulsification, and tetraethyl orthosilicate (TEOS) as the cross-linking agent, along with co-functional monomers 3-aminopropyltriethoxysilane (APTES) and benzyl(triethoxy)silane (BnTES), enhanced binding efficacy was achieved. Successful imprinting was evidenced through surface morphology observation and physical/chemical property evaluations of the synthesized MIPs. A series of adsorption experiments were performed to investigate the recognition performance of Hb-MIPs. The Hb-MIPs not only exhibited large adsorption capacity (400 μg/mg) and good imprinting factor (6.09) toward template protein, but also showed satisfactory selectivity for reference proteins. Five cycles of adsorption proved that the Hb-MIPs had good reusability. In addition, the successful isolation of HB from bovine blood indicated that Hb-MIPs were an excellent separation and purification material. The mild preparation conditions and good adsorption capacity demonstrated the potential value of this method in separation and purification research.

Molecularly imprinted thermosensitive probe based on fluorescent advanced glycation end products to detect α-dicarbonyl compounds and inhibit pyrraline formation.

A thermal-sensitive molecularly imprinted optosensing probe based on fluorescent advanced glycation end products (AGEs) was prepared by one-pot hydrothermal synthesis. Carbon dots (CDs) derived from fluorescent AGEs were used as the luminous centers, while molecularly imprinted polymers (MIPs) were wrapped outside of theCDs to form specific target recognition sites to highly selectively adsorbthe intermediate product of AGEs of 3-deoxyglucosone (3-DG). Thermosensitive N-isopropylacrylamide (NIPAM) was combined with acrylamide (AM) as co-functional monomers, and ethylene glycol dimethacrylate (EGDMA) was chosen as across-linker for targeting identification and detection of 3-DG. Under optimal conditions, the fluorescence of MIPs could be gradually quenched with the adsorption of 3-DG on the surface of MIPs in the linear range of 1-160μg/L, and the detection limit was 0.31μg/L. The spiked recoveries of MIPs ranged from 82.97 to 109.94% in two milk samples, and the relative standard deviations were all less than 1.8%. In addition, the inhibition rate for non-fluorescent AGEs of pyrraline (PRL) was 23% by adsorbing 3-DG in the simulated milk system of casein and D-glucose, indicating that temperature-responsive MIPs not only could detect the dicarbonyl compound 3-DG quickly and sensitively, but also had an excellent inhibitory effect on AGEs.

Hydrophilic molecularly imprinted chitosan microspheres based on deep eutectic solvents for micro-solid phase extraction of catechin

Hydrophilic molecularly imprinted chitosan microspheres (HMICS) were synthesized using deep eutectic solvents (DESs)-(3-aminopropyl) triethoxysilane (APTES) as co-functional monomer for the extraction of catechin from green tea with micro-solid phase extraction (micro-SPE). Various factors were studied to optimize the extraction condition during extraction process. The actual extraction amount under the optimal conditions (amount of adsorbent (20 mg), type and volume of washing (acetone, 1.2 mL) and eluting solvent (acetone/acetic acid, 1.0 mL), extraction time (30 min), extraction temperature (40 °C), and sample pH (4)) were obtained at 97.06 mg·g−1. The catechin recovery ranged with ranged with limit of detection (LOD) within 0.09–0.24 µg·mL−1, and limit of quantification (LOQ) within 0.18–0.28 µg·mL−1. From the experimental results in this system, the HMICS was proved to have higher extraction capacity, improving as a promising adsorbent for the extraction of catechin.

Highly selective removal of kitasamycin from the environment by molecularly imprinted polymers: Adsorption performance and mechanism

A newly green molecularly imprinted polymer (MIP) for kitasamycin (KITA) was synthesized in water by using 1-allyl-3-vinylimidazolium chloride ionic liquid and 2-acrylamide-2-methylpropanesulfonic acid as co-functional monomer on the surface of poly (styrene-divinylbenzene) particles. The prepared MIP has excellent selectivity for KITA in mixed solution, and approximately 90% of KITA can be bounded onto MIP within the first 30 min. And the MIP exhibited powerful affinity towards KITA with the maximum adsorption amount of 127.06 mg g -1 with the recovery of 92.3–108.8%, and the binding capacity increased within the contact temperature of 25–45 °C. The adsorption mechanism revealed that the synergistic effect of hydrogen bond, π-π interaction and molecule structure was attributed to selectivity of the MIP. Under the optimized condition, a MIP-SPE cartridge coupled with high performance liquid chromatography (HPLC) was developed to determine the residues of KITA in soil, water and muscle samples, and the MIP-SPE cartridge can be regenerated at least 25 cycles. Therefore, the developed MIP-SPE-HPLC strategy can be used as a specific, cost-effective and environmentally-friendly method for identification and determination KITA in environmental samples. A novel eco-friendly molecularly imprinted polymer (MIP) was proposed in water by using 1-allyl-3-vinylimidazolium chloride and 2-acrylamide-2-methylpropanesulfonic acid as bifunctional monomer for the specific adsorption of kitasamycin (KITA). The obtained MIP had excellent selectivity towards KITA and can be used as solid phase extraction for recognizing and separating trace KITA in soil, water and muscle samples. • An eco-friendly hydrophilic kitasamycin MIP was synthesized. • The MIP showed excellent selectivity and adsorption capacity to KITA in water. • The excellent practicability and reusability of MIP-SPE were also demonstrated.

Fabrication of molecularly imprinted polymers with tunable adsorption capability based on solvent-responsive cross-linker

The fabrication of facile and versatile adsorbents with switchable adsorption performance based on molecularly imprinting polymers is of great interest although still very challenging. Herein, a dynamic solvent-responsive poly (ethylene glycol) unit-containing chain was introduced as cross-linker to prepare solvent-responsive molecularly imprinted polymers (S-MIPs) by free radical polymerization. Acryloyl-modified beta-cyclodextrin was used as functional monomer, methacrylic acid as co-functional monomer and bisphenol A (BPA) as model template. BPA can be tightly adsorbed in recognition sites by host-guest interaction and hydrogen binding. Solvent-responsiveness of S-MIPs was investigated. Templates can be easily bound into the recognition cavity in 25% methanol aqueous solution with binding capacity of 21.36 mg/g while completely release in 100% methanol following the de-swelling of the imprinting cavity. Binding experiments demonstrated that S-MIPs displayed appreciable recognition capability and selectivity toward BPA in 25% methanol solution, well lining with Langmuir isothermal model and pseudo-second-order model. The proposed S-MIPs were successfully applied for the direct enrichment of BPA from human urine without protein pre-deposition process. Under the optimum conditions, the S-MIPs exhibited satisfactory recoveries ranging from 77.3% to 87.8% (spiked level from 40 μg/L to 800 μg/L), indicating excellent applicability for specific analytes removal from urine samples. This simple synthetic strategy provides an efficient route to synthesize the new intelligent materials for sample analysis in complicated biological matrixes.

Extraction of activated epimedium glycosides in vivo and in vitro by using bifunctional-monomer chitosan magnetic molecularly imprinted polymers and identification by UPLC-Q-TOF-MS

In this work, efficient, sensitive bifunctional-monomer chitosan magnetic molecularly imprinted polymers (BCMMIPs) were fabricated and successfully applied to concentrate the metabolites of Epimedium flavonoids in rat testis and bone that were later analyzed using UPLC-Q-TOF-MS. Using chitosan and methacrylic acid as co-functional monomers, BCMMIPs exhibited a large adsorption capacity (7.60 mg/g), fast kinetics (60 min), and good selectivity. Chitosan is bio-compatible and non-toxic, and methacrylic acid provides multiple hydrogen bond donors. The BCMMIPs were injected into rat testis to specifically enrich the total flavonoid metabolites in vivo and were used to extract metabolites from bone in vitro. The results showed that the BCMMIPs coupled with UPLC-Q-TOF-MS successfully identified 28 compounds from testis and 18 compounds from bone, including 19 new compounds. This study provided a reliable protocol for the concentration of metabolites from complex biological samples, and several new metabolites of Epimedium flavonoids were found in vivo and in vitro.

One-step polymerization of hydrophilic ionic liquid imprinted polymer in water for selective separation and detection of levofloxacin from environmental matrices.

A novel green hydrophilic levofloxacin imprinted polymer was presented via one-step polymerization in water using ionic liquid 1,6-hexa-3,3'-bis-1-vinylimidazolium bromine with multiple hydrophilic groups and 2-hydroxyethyl methacrylate as a co-functional monomer. Adsorption experiment revealed that the ionic liquid significantly improved the water compatible of imprinted polymer, and the excellent recognition of molecularly imprinted polymer for levofloxacin in water corresponds to the synergetic effect of H-bonding and the electrostatic and π-π interactions between the levofloxacin and co-functional monomer. Furthermore, the adsorption process of the imprinted material towards levofloxacin fitted the Langmuir model, and the maximum binding amount of levofloxacin onto the imprinted and corresponding non-imprinted polymer were 16.45 and 6.82mg/g at 25°C, respectively. After optimizing the parameters affecting solid phase extraction performance, an enrichment and determination system was achieved to separate and detect levofloxacin from water and sediment samples with recoveries that ranged from 83.67 to 101.33% and relative standard deviation of less than 5.59%.

Synthesis and applications of epoxy-functionalized bi-functional magnetic molecularly imprinted polymers for polysaccharide adsorption

A polysaccharide-imprinted nanoparticle composite material with bi-functional monomers molecularly imprinted polymers (Bi-MMIPs) was successfully synthesized in aqueous solution by using starch as the template, 3-aminobenzeneboronic acid (APBA) as the functional monomer, 2-acrylamide-2-methylpropanesulfonic acid (AMPS) as the co-functional monomer, and ammonium persulfate (APS) as the initiator. Transmission electron microscopy (TEM), scanning electron microscopy (SEM), and Fourier-transform infrared (FT-IR) spectroscopy were used to characterized Bi-MMIPs. The adsorption and recognition characteristics of Bi-MMIPs for starch were investigated in detail by using the static method, dynamic method, and competitive adsorption experiments. The TEM, SEM and FT-IR results showed that the two functional monomers were loaded on the surface of Bi-MMIPs. Bi-MMIPs possessed very strong adsorption affinity and specific recognition for starch. The saturated adsorption capacity reached 13.88 mg/g, and the selectivity coefficients relative to glucosans Mr 5000 Da and 70000 Da were 2.67 and 3.77, respectively. Despite the imprinting factor of Bi-MMIPs (α=3.04), Bi-MMIPs were regenerated easily and exhibited excellent recognition, selectivity, and reusability for adsorbing starch. To elucidate the mechanism, APBA and AMPS showed synergy effect in synthesizing bi-functional monomers by providing reversible covalent bonds and hydrogen bonds, respectively, which could effectively enhance the spatial arrangement of combining sites for template molecular.

Detection of Bisphenol A in aqueous medium by screen printed carbon electrodes incorporating electrochemical molecularly imprinted polymers

Electrochemical molecularly imprinted polymers (e-MIPs) were for the first time introduced in screen-printed carbon electrodes (SPCE) as the sensing element for the detection of an organic pollutant. To play this sensing role, a redox tracer was incorporated inside the binding cavities of a cross-linked MIP, as a functional monomer during the synthesis step. Ferrocenylmethyl methacrylate was used for this purpose. It was associated with 4-vinylpyridine as a co-functional monomer and ethylene glycol dimethacrylate as cross-linker for the recognition of the endocrine disruptor, Bisphenol A (BPA), as a target. Microbeads of e-MIP and e-NIP (corresponding non-imprinted polymer) were obtained via precipitation polymerization in acetonitrile. The presence of ferrocene inside the polymers was assessed via FTIR and elemental analysis and the polymers microstructure was characterized by SEM and nitrogen adsorption/desorption experiments. Binding isotherms and batch selectivity experiments evidenced the presence of binding cavities inside the e-MIP and its high affinity for BPA compared to carbamazepine and ketoprofen. e-MIP (and e-NIP) microbeads were then incorporated in a graphite-hydroxyethylcellulose composite paste to prepare SPCE. Electrochemical properties of e-MIP-SPCE revealed a high sensitivity in the presence of BPA in aqueous medium compared to e-NIP-SPCE with a limit of detection (LOD) of 0.06 nM. Selectivity towards carbamazepine and ketoprofen was also observed with the e-MIP-SPCE.

Synthesis of molecularly imprinted polymers using acrylamide-β-cyclodextrin as a cofunctional monomer for the specific capture of tea saponins from the defatted cake extract of Camellia oleifera.

Molecularly imprinted polymers were synthesized using mixed tea saponins as a template and acrylamide-β-cyclodextrin as a cofunctional monomer for the specific binding and purification of tea saponins from the defatted cake extract of Camellia oleifera. The adsorption properties of the prepared polymers were systematically evaluated including adsorption kinetics, adsorption isotherms, and selective recognition characteristics. It showed that the adsorption kinetics followed the pseudo first-order kinetic model (R2 = 0.995) with an equilibrium time of 3 h, adsorption isotherm data fitted well with the Langmuir-Freundlich model (R2 = 0.984) with an adsorption capacity of 14.23mg/g. The relative selectivity coefficient (k´) in the presence of the analogues glycyrrhizic acid and glycyrrhetinic acid were 1.16 and 17.21, respectively. The performance of the molecularly imprinted polymers as solid-phase extraction materials was investigated and the results indicated that using acrylamide-β-cyclodextrin as a cofunctional monomer improved both the adsorption capacity and active sites stability of the imprinted polymers. The solid-phase extraction using the polymers as packing materials was subsequently applied for the separation of tea saponins in raw C. oleifera press extract, and targets were obtained with a purity reaching 89%.

Molecularly imprinted ionic liquid magnetic microspheres for the rapid isolation of organochlorine pesticides in environmental water.

A new type of molecularly imprinted ionic liquid magnetic microspheres was synthesized by aqueous suspension polymerization, using 4,4'-dichlorobenzhydrol as a dummy template, and 1-allyl-3-ethylimidazolium hexafluorophosphate and methacrylic acid as co-functional monomers. The results of morphology and magnetic property evaluation of the obtained microspheres demonstrated that it was monodispersed spherical, possessed a rough surface, and an outstanding magnetic properties. Binding experiments revealed that it had a substantial adsorption capacity and strong recognition ability to organochlorine pesticides (OCPs) in aqueous solution. Then the microspheres were applied as an adsorbent of magnetic dispersive solid-phase extraction for the selective recognition and rapid determination of OCPs in environmental water. Under the optimum conditions, good linearity of the three types of OCPs (dicofol, tetradifon, and p,p'-dichlorodiphenyldichloroethane) was achieved in the range of 1.0-100 ng/mL (r ≥ 0.9994). The recoveries at three spiking levels ranged from 82.6 to 100.4% with the RSDs less than 6.9%.

Preparation of Cu2+-mediated magnetic imprinted polymers for the selective sorption of bovine hemoglobin

In this work, a novel Cu2+-mediated core–shell bovine hemoglobin imprinted superparamagnetic polymers were synthesized. First, carboxyl group directly-functionalized Fe3O4 nanoparticles were produced by a facile one-pot hydrothermal method. Next, copper ions were introduced to chelate with carboxyl groups and further bonded with template bovine hemoglobin as co-functional monomer. Then, functional monomers 3-aminopropyltriethoxylsilane and octyltrimethoxysilane were adopted to form the thin polymer layers. Finally, after removal of the templates, the imprinting shells with specific recognition cavities for bovine hemoglobin were obtained on Fe3O4 nanoparticles. The resultant molecularly imprinted polymers have high adsorption capacity and satisfactory selectivity for bovine hemoglobin with the help of copper ions. The obtained magnetic nanomaterials were characterized by transmission electron microscopy, Fourier-transform infrared spectra, X-ray diffraction, and vibrating sample magnetometer. The measurements demonstrated that the as-synthesized nanomaterials exhibited good dispersion, high crystallinity, and satisfactory superparamagnetic properties. The feasibility of this method was further confirmed by using the imprinted nanomaterials to specifically extract bovine hemoglobin from real bovine blood samples.

Preparation and evaluation of Fe $$_{3}$$ 3 O $$_{4}$$ 4 nanoparticles incorporated molecularly imprinted polymers for protein separation

Protein imprinting is still a challenge due to the low binding kinetics and poor binding selectivity. In this study, a facile method of the preparation of magnetic molecularly imprinted polymers (MIPs) for selective protein separation was reported. Carboxyl group functionalized Fe $$_{3}$$ O $$_{4}$$ nanoparticles (NPs) were synthesized using a solvothermal method. After pre-assembly of caroxyl group functionalized Fe $$_{3}$$ O $$_{4}$$ NPs and template protein lysozyme (Lyz) to form Lyz–Fe $$_{3}$$ O $$_{4}$$ complex, magnetic MIPs were synthesized by a sol–gel process of 3-aminopropyltriethoxylsilane and tetraethyl silicate with Lyz–Fe $$_{3}$$ O $$_{4}$$ complex incorporated. Then Fe $$_{3}$$ O $$_{4}$$ –MIPs particles with magnetic response could be collected by simple crush of bulk polymers. This preparation process avoid the need of high dilution of monomer for anti-agglomeration in the surface imprinting, and large amount of solvent is spared. The morphology and structure property of the prepared magnetic NPs were characterized by transmission electronic microscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometer. Binding experiments were carried out to evaluate Fe $$_{3}$$ O $$_{4}$$ –MIPs particles’ binding performance and selectivity. And results showed fast binding kinetics, high binding capacity, and favorable specific recognition behavior toward template protein, which is due to the role of carboxyl group functionalized Fe $$_{3}$$ O $$_{4}$$ NPs as both magnetic source and importantly as co-functional monomer incorporated in the polysiloxane imprinting system. Real egg white sample tests demonstrate good separation effect. This report provides a possibility of the selective separation of protein in complex matrix.

A highly-efficient imprinted magnetic nanoparticle for selective separation and detection of 17β-estradiol in milk

In this work, we prepared molecularly imprinted polymers (MIPs) combining surface molecular imprinting technique and magnetic separation for separation and determination of 17β-estradiol (E2) from milk. During the synthesis process, the acryloyl chloride was specially used to graft double bonds on Fe3O4 nanoparticles and served as co-functional monomer cooperating with acrylamide. The morphology, structure property, and the best polymerization and adsorption conditions of the prepared magnetic nanoparticles were investigated in detail. The obtained nanomaterials displayed high adsorption capacity of 12.62mg/g, fast equilibrium time of 10min, and satisfactory selectivity for target molecule. What’s more, the MIPs was successfully applied as sorbents to specifically separate and enrich E2 from milk with a relatively high recovery (88.9–92.1%), demonstrating the potential application of the MIPs as solid phase extractant for rapid, highly-efficient, and cost-effective sample analysis.

Ionic liquid-molecularly imprinted polymers for pipette tip solid-phase extraction of (Z)-3-(chloromethylene)-6-flourothiochroman-4-one in urine

A new type of ionic liquid-molecularly imprinted polymers (IL-MIPs) synthesized by precipitation polymerization using 1-allyl-3-methylimidazolium bromide and acrylamide as co-functional monomers and (Z)-6-fluoro-3-(hydroxymethylene)-thiochroman-4-one (FHO) as dummy template was successfully applied as a selective adsorbent of pipette tip solid-phase extraction (PT-SPE) for rapid extraction and determination of (Z)-3-(chloromethylene)-6-flourothiochroman-4-one (CFO) in urine. The factors that affected the extraction efficiency including amounts of adsorbent, types of washing solvent, and types and volumes of elution solvent were optimized. Under the optimum conditions of ionic liquid-molecularly imprinted polymers-pipette tip solid-phase extraction (IL-MIPs-PT-SPE) coupled with HPLC-UV, good linearity for CFO was achieved in a range of 0.1–10.0μgmL−1 (r=0.9999) and the recoveries at three spiked levels were ranged from 94.8% to 103.4% with the relative standard deviations (RSDs) less than 5.7% (n=3).

A novel bifunctional molecularly imprinted polymer for determination of Congo red in food

A novel bifunctional molecularly imprinted polymer was synthesized with beta-cyclodextrins-maleic anhydride and [2-(methacryloyloxy) ethyl] trimethylammonium chloride as co-functional monomers, and applied to the determination of food.

Preparation and application of sulfadiazine surface molecularly imprinted polymers with temperature‐responsive properties

ABSTRACTNovel, temperature‐responsive molecularly imprinted polymers (TMIPs) based on potassium hexatitanate whiskers for selectively adsorbing sulfadiazine (SDZ) from aqueous media were prepared with methacrylic acid (MAA) and 4‐vinylpyridine (4‐VP) as cofunctional monomers and N‐isopropyl acrylamide (NIPAM) as a temperature‐responsive monomer. The template–monomer interactions were studied by molecular simulation. In particular, the effects of different kinds of crosslinkers on the selective recognition ability of the TMIPs in water media were investigated. The temperature–responsive adsorption performance and phase behavior of the molecularly imprinted polymers were studied by batch‐mode binding experiments, swelling experiments, and contact angle testing. The results demonstrate that the combination of MAA, 4‐VP, and NIPAM was a favorable temperature‐responsive imprinted system for SDZ in water, and the cocrosslinking agent of ethylene glycol dimethacrylate (EGDMA) and N,N′‐methylene bisacrylamide (MBA) was more suitable compared with either pure EGDMA or MBA. The adsorption kinetics and adsorption isotherms were analyzed by the fitting of different adsorption models. Also, the effect of the temperature on the recovery was investigated by the determination of the spiked SDZ in real‐water samples with solid‐phase extraction and high‐performance liquid chromatography. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41769.

Preparation of ionic liquid polymer materials and their recognition properties for proteins

In this paper, two polymer materials were synthesized by using 1-allyl-3-butylimidazolium chloride ([ABIM][Cl]) or 1-vinyl-3-octylimidazolium bromide ([VOIM][Br]) as functional monomers, and arcylamide (AAm) as a co-functional monomer. Bovine serum albumin (BSA), bovine hemoglobin (BHb), lysozyme (Lyz), and cytochrome C (Cyt C) that have different properties were selected as model proteins to investigate the absorption and separation abilities of ionic liquids polymer materials. The selective absorption properties of two polymer materials for proteins were compared for the first time. The results showed that [ABIM][Cl] ionic liquid polymer material had a high binding capacity for BHb (828.5 mg g−1), and [VOIM][Br] polymer material possessed a high binding capacity for BSA (804.7 mg g−1). Different proteins can be separated by altering the cations of the ionic liquid. In addition, the structure, morphology and thermal properties of ILs polymer materials were characterized by scanning electron microscope (SEM), fourier transform infrared (FT-IR) spectroscopy, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). These results indicated that the IL polymer materials created a tendency for the partition of proteins efficiently and may be applied in medical diagnosis, proteomics, biotechnology and sensors broadly.

A novel free-standing flexible molecularly imprinted membrane for selective separation of synephrine in methanol–water media

Flexibility was very important for molecularly imprinted membrane (MIM) to apply to practical use. A novel free-standing flexible MIM was prepared using methacrylic acid (MAA) and 2-hydroxyethyl acrylate (HEA) as co-functional monomer, and ionic liquid as porogen. HEA was able to effectively improve the flexibility of MIM. Ionic liquid, 1-butyl-3-methylimidazolium bromide, was able to accelerate the synthesis process and improve the selectivity and adsorption of the MIM. The properties of MIM were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR), thermo gravimetric analysis (TGA), differential thermal analysis (DTA) and X-ray photoelectron spectroscopy (XPS). Two structurally similar compounds (octopamine and tyramine) were selected as competitors to examine the selective recognition of MIM to synephrine (SYN). The competitive adsorption and permeation selectivity experiments showed that the MIM could selectively recognize SYN, and was able to be used to selectively separate SYN from the standard mixtures or the extract of stir-baked Aurantii Fructus Immaturus in methanol/water (4:1, v/v) media.

Protein imprinted ionic liquid polymer on the surface of multiwall carbon nanotubes with high binding capacity for lysozyme.

In this research, ionic liquid as functional monomer to prepare molecularly imprinted polymers for protein recognition was for the first time demonstrated, in which, 1-vinyl-3-butylimidazolium chloride was selected as functional monomer, acrylamide as co-functional monomer and lysozyme (Lyz) as template protein to synthesize imprinted polymers on the surface of multiwall carbon nanotubes in aqueous medium. The results indicated that ionic liquid was helpful to improve binding capacity of imprinted polymers, which had a maximum binding capacity of 763.36 mg/g in the optimum adsorption conditions. The prepared imprinted polymers had a fast adsorption rate and a much higher adsorption capacity than the corresponding non-imprinted polymers, with the difference in adsorption capacity up to 258.31 mg/g. The obtained polymer was evaluated by Lyz, bovine serum albumin (BSA), bovine hemoglobin (BHb), equine myoglobin (MB) and cytochrome c (Cyt c). The selectivity factor (β) for Lyz/BSA, Lyz/Mb, Lyz/BHb, and Lyz/Cyt c were 7.17, 2.12, 1.76 and 1.57, respectively, indicating the imprinted polymers had a good selectivity. Easy preparation of the imprinted polymers as well as high ability and selectivity to adsorb template proteins makes this polymer attractive and broadly applicable in biomacromolecular separation, biotechnology and sensors.