To evaluate the acute effect of exposure to a potentiation warm-up protocol compared to a usual warm-up program. Randomized parallel control trial. Synthetic grass in the club's facilities (Portugal). Seventeen female football players (age: 23.9 ± 3.9 years), were randomly allocated to a control (n = 8) and an experimental group (n = 9). To allocate the players, a table was computer-generated by a research team member with no involvement in the trial. The control group performed their usual warm-up program, while the experimental group performed a potentiation warm-up protocol with jumps combined with sprints with change of direction. The players were tested pre- and post-intervention for a 40-m linear sprint and pre-planned change of direction using the T-test. An intention-to-treat analysis was performed, with all the participants originally randomized being involved. The normal distribution was verified by the Shapiro-Wilk test. The assumption of sphericity was analyzed. Effect sizes were calculated using partial eta squared. No significant pre-post differences in the T-test and in the 40-m sprint were detected for any group. However, in the T-test, large effect sizes in time increments were observed within the experimental (0.27s; p > 0.05, ηp2 = 0.176) and control groups (0.06s; p > 0.05, ηp2 = 0.176). Also, in the 40-m sprint, large effect sizes in time increments were observed within the experimental (0.05s; p > 0.05, ηp2 = 0.251) and control groups (0.09s; p > 0.05, ηp2 = 0.251). The performance-enhancing ability of the potentiation method performed at warm-up was not verified when applied to female football players. Thus, the potentiation methods may not improve sprint and COD ability for this population. However, the lack of statistical significance may have been due to reduced statistical power, as three of four effects suggest acute performance impairment after a supposed potentiation-oriented warm-up. Nevertheless, the presence of a statistical type 2 error cannot be ruled out. NCT06555185; Project URL: https://clinicaltrials.gov/study/NCT06555185 .
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