Estimation of radio refractivity is important in the planning and design of terrestrial radio communication links for the availability and accessibility of strong networks and signals. This paper investigates the refractivity gradient, effective earth radius factor (k), and the geo-climatic factor K in the first 1km of the troposphere of two selected stations (Maiduguri and Enugu) under different climatic zones in Nigeria. The indirect method of measuring radio refractivity was employed in this study to take measurements over the two selected stations. Vertical profile values of pressure (hPa), Temperature (°C), and Relative humidity (%) within the first 1 km were extracted from MERRA MAIMCPASM V5.20 database profile obtained from a satellite sounding instrument by NASA in the United States. MatLab programming language was used to evaluate the refractivity gradient, k-factor, and geo-climatic factor using the equations recommended by ITU. The results showed that Enugu was predominantly sub-refractive due to the tropical savannah climate while Maiduguri encountered both sub-refractive and normal refractive conditions due to the hot semi-arid climate, and unstable and extreme weather conditions in the region.