Climate Change-induced Stress Research Articles

Molecular Communication of Microbial Plant Biostimulants in the Rhizosphere Under Abiotic Stress Conditions.

Microbial plant biostimulants offer a promising, sustainable solution for enhancing plant growth and resilience, particularly under abiotic stress conditions such as drought, salinity, extreme temperatures, and heavy metal toxicity. These biostimulants, including plant growth-promoting rhizobacteria, mycorrhizal fungi, and nitrogen-fixing bacteria, enhance plant tolerance through mechanisms such as phytohormone production, nutrient solubilization, osmotic adjustment, and antioxidant enzyme activation. Advances in genomics, metagenomics, transcriptomics, and proteomics have significantly expanded our understanding of plant-microbe molecular communication in the rhizosphere, revealing mechanisms underlying these interactions that promote stress resilience. However, challenges such as inconsistent field performance, knowledge gaps in stress-related molecular signaling, and regulatory hurdles continue to limit broader biostimulant adoption. Despite these challenges, microbial biostimulants hold significant potential for advancing agricultural sustainability, particularly amid climate change-induced stresses. Future studies and innovation, including Clustered Regularly Interspaced Short Palindromic Repeats and other molecular editing tools, should optimize biostimulant formulations and their application for diverse agro-ecological systems. This review aims to underscore current advances, challenges, and future directions in the field, advocating for a multidisciplinary approach to fully harness the potential of biostimulants in modern agriculture.

Agroforestry as a Sustainable Solution for Mitigating Climate Change Impacts and Addressing Water Scarcity in Tunisia: A Review

Agroforestry, as a sustainable land management practice, has garnered attention worldwide for its potential to mitigate climate change impacts and address water scarcity issues. In Tunisia, where climate change-induced stresses are increasingly affecting agriculture and water resources, agroforestry presents a promising solution. This review paper examines the importance of agroforestry in Tunisia within the context of climate change, water shortage, and treated wastewater utilization. By analyzing existing literature and case studies, the paper elucidates the ecological, economic, and social benefits of agroforestry practices in Tunisia. Additionally, it discusses the role of agroforestry in enhancing resilience to climate change, conserving water resources, and utilizing treated wastewater sustainably. Furthermore, the paper evaluates policy support and identifies challenges hindering the widespread adoption of agroforestry in Tunisia. Overall, this review underscores the significance of agroforestry as a multifaceted approach to sustainable land management in Tunisia, offering recommendations for policymakers, practitioners, and researchers to promote its implementation and integration into national strategies.

Successional changes in bacterial phyllosphere communities are plant-host species dependent.

Leaf surface microbiomes have the potential to influence agricultural and ecosystem productivity. We assessed their stability by determining composition, functional resistance, and resilience. Resistance is the degree to which communities remain unchanged as a result of disturbance, and resilience is the ability of a community to recover to pre-disturbance conditions. By understanding the mechanisms of community assembly and how they relate to the resistance and resilience of microbial communities under common environmental stresses such as drought, we can better understand how communities will adapt to a changing environment and how we can promote healthy agricultural microbiomes. In this study, phyllosphere compositional stability was highly related to plant host species phylogeny and, to a lesser extent, known stress tolerances. Phyllosphere community assembly and stability are a result of complex interactions of ecological processes that are differentially imposed by host species.

Impacts of Climate Change on Soil Microbial Interactions: Echoes of the New Normal

<p>Concerns over the negative impacts of climate change on ecosystems and human life have entered a new phase where many hypothetical views are fast becoming realities. Presently, the rampaging effect of climate change is, in theory, causing ecological catastrophes, and it is being felt at an alarming scale worldwide. As an important ecological niche, the soil ecosystem hosts a diversity of microbiomes and macrobiomes and affords a soil-plant-microbes ecological continuum. Also, it supports essential ecological processes meant to promote life-sustaining habits. However, changes in plant diversity due to increasing greenhouse effects, anthropogenic activities, and global warming have severely impacted the stability of soil microbial communities and interactions, particularly the soil-plant-microbe interaction. A good understanding of the mechanisms underpinning the plant-soil-microbial interactions, the complexity of the soil microbiome, ecosystem adaptability to climate change-induced stresses, and niche functionality of microbiota is necessary for the empirical impact assessment of climate change on soil microbial behaviors. Moreover, the soil system parameters and the various ecological services affected need to be further studied to identify opportunities that could assist the quest to mitigate the debilitating effects of climatic change in the soil ecosystem and sustainable food security initiatives.</p>

Early warning signals indicate climate change-induced stress in Norway spruce in the Eastern Carpathians

Climate change is affecting forest ecosystems globally, in particular through warming as well as increases in the frequency and intensity of extreme events. Norway spruce (Picea abies (L.) Karst.) is one of the most important coniferous tree species in Europe. In recent extremely dry years in Central Europe, spruce suffered and large dieback has been observed. In parts of Eastern Europe, however, no large-scale decline in spruce has been reported so far, though anticipated changes in climate pose the question how the future of these forests may look like.To assess the current state of spruce forests in Eastern Europe, we established a tree-ring network consisting of 157 Norway spruce chronologies (from >3000 trees) of different ages distributed along elevational transects in the Eastern Carpathians, Romania. We evaluated early warning signals of climate change-induced stress, i.e. (1) growth decline, (2) increased sensitivity of tree growth (assessed over the statistics first-order autocorrelation and standard deviation), and (3) increased growth synchrony.A pronounced growth decline was observed over the last two decades, which was strongest in younger stands and at lower elevations. However, growth sensitivity and synchrony did not show consistent patterns, suggesting that forest decline may not be immediately imminent. Overall, our findings highlight an increased vulnerability of spruce in the Eastern Carpathians. With ongoing climate change, spruce dieback may be expected in this part of Europe as well.

Aureobasidium pullulans Treatment Mitigates Drought Stress in Abies koreana via Rhizosphere Microbiome Modulation.

The Korean fir tree Abies koreana, an endangered species in Korea, faces threats primarily from climate change-induced stress and drought. This study proposed a sustainable method to enhance A. koreana drought tolerance using a black yeast-like fungus identified as Aureobasidium pullulans (AK10). The 16S/ITS metabarcoding analysis assessed the impact of drought and AK10 treatment on the seedlings' rhizosphere microbiome. Results revealed a profound drought influence on the microbiome, particularly affecting fungal mycobiota. Drought-stressed seedlings exhibited elevated Agaricaceae levels, opportunistic fungi generally associated with decomposition. AK10 treatment significantly mitigated this proliferation and increased the relative abundance of beneficial fungi like Cystofilobasidium and Mortierella, known biocontrol agents and phosphate solubilizers. A notable reduction in the phytopathogenic Fusarium levels was observed with AK10, alongside an increase in beneficial bacteria, including Azospirillum and Nitrospirillum. Furthermore, the conducted correlation analysis shed light on microbial interrelationships within the rhizosphere, elucidating potential co-associations and antagonisms. Taken together, the isolated A. pullulans AK10 identified in this study serves as a potential biostimulant, enhancing the drought tolerance in A. koreana through beneficial alterations in the rhizosphere microbiome. This approach presents a promising strategy for the conservation of this endangered species.

Impacts of ocean warming and acidification on predator-prey interactions in the intertidal zone: A research weaving approach

The effect of ocean warming and acidification on predator-prey interactions in the intertidal zone is a topic of growing concern for the scientific community. In this review, we aim to describe how scientists have explored the topic via research weaving, a combination of a systematic review, and a bibliometric approach. We assess articles published in the last decade exploring the impact of both stressors on predation in the intertidal zone, via experimental or observational techniques. Several methods were used to delve into how climate change-induced stress affected intertidal predation, as the study design leaned toward single-based driver trials to the detriment of a multi-driver approach. Mollusks, echinoderms, and crustaceans have been extensively used as model organisms, with little published data on other invertebrates, vertebrates, and algae taxa. Moreover, there is a strong web of co-authoring across institutions and countries from the Northern Hemisphere, that can skew our understanding towards temperate environments. Therefore, institutions and countries should increase participation in the southern hemisphere networking, assessing the problems under a global outlook. Our review also addresses the various impacts of ocean acidification, warming, or their interaction with predation-related variables, affecting organisms from the genetic to a broader ecological scope, such as animal behaviour or interspecific interactions. Finally, we argue that the numerous synonyms used in keywording articles in the field, possibly hurting future reviews in the area, as we provide different keyword standardizations. Our findings can help guide upcoming approaches to the topic by assessing what has been already done and revealing gaps in emerging themes, like a strong skew towards single-driver (specially acidification) lab experiments of northern hemisphere organisms and a lack of field multi-stressor experiments.

Climate change–induced stress disrupts ectomycorrhizal interaction networks at the boreal–temperate ecotone

The interaction networks formed by ectomycorrhizal fungi (EMF) and their tree hosts, which are important to both forest recruitment and ecosystem carbon and nutrient retention, may be particularly susceptible to climate change at the boreal-temperate forest ecotone where environmental conditions are changing rapidly. Here, we quantified the compositional and functional trait responses of EMF communities and their interaction networks with two boreal (Pinus banksiana and Betula papyrifera) and two temperate (Pinus strobus and Quercus macrocarpa) hosts to a factorial combination of experimentally elevated temperatures and reduced rainfall in a long-term open-air field experiment. The study was conducted at the B4WarmED (Boreal Forest Warming at an Ecotone in Danger) experiment in Minnesota, USA, where infrared lamps and buried heating cables elevate temperatures (ambient, +3.1 °C) and rain-out shelters reduce growing season precipitation (ambient, ~30% reduction). EMF communities were characterized and interaction networks inferred from metabarcoding of fungal-colonized root tips. Warming and rainfall reduction significantly altered EMF community composition, leading to an increase in the relative abundance of EMF with contact-short distance exploration types. These compositional changes, which likely limited the capacity for mycelial connections between trees, corresponded with shifts from highly redundant EMF interaction networks under ambient conditions to less redundant (more specialized) networks. Further, the observed changes in EMF communities and interaction networks were correlated with changes in soil moisture and host photosynthesis. Collectively, these results indicate that the projected changes in climate will likely lead to significant shifts in the traits, structure, and integrity of EMF communities as well as their interaction networks in forest ecosystems at the boreal-temperate ecotone.

Pseudomonas spp. can help plants face climate change.

Climate change is increasingly affecting agriculture through droughts, high salinity in soils, heatwaves, and floodings, which put intense pressure on crops. This results in yield losses, leading to food insecurity in the most affected regions. Multiple plant-beneficial bacteria belonging to the genus Pseudomonas have been shown to improve plant tolerance to these stresses. Various mechanisms are involved, including alteration of the plant ethylene levels, direct phytohormone production, emission of volatile organic compounds, reinforcement of the root apoplast barriers, and exopolysaccharide biosynthesis. In this review, we summarize the effects of climate change-induced stresses on plants and detail the mechanisms used by plant-beneficial Pseudomonas strains to alleviate them. Recommendations are made to promote targeted research on the stress-alleviating potential of these bacteria.

Hydrogeological modeling of the Roussillon coastal aquifer (France): stochastic inversion and analysis of future stresses

Global climate change-induced stresses on coastal water resources include water use restrictions, saline intrusions, and permanently modifying or damaging regional resources. Groundwater in coastal regions is often the only freshwater resource available, so an in-depth understanding of the aquifer, and the aquifer’s response to climate change, is essential for decision-makers. In this study, we focus on the coastal aquifer of Roussillon (southern France) by developing and investigating a steady-state groundwater flow model (MODFLOW 6) and calibrated with PEST++ on a Python interface (FloPy and PyEmu). Model input and boundary conditions are constrained by various scenarios of climate projections by 2080, with model results predicting the aquifer’s response (and associated uncertainty) to these external forcings. Using simple assumptions of intrusion estimates, model results highlight both strong climatic and anthropogenic impacts on the water table. These include aquifer drawdowns reaching several meters locally, and the seawater interface advancing locally several hundred meters inland and rising by several meters. Intrusions of this magnitude risk endangering exploited water wells and their sustainability. Our results demonstrate the critical importance of properly characterizing the geology and its heterogeneity for understanding aquifers at risk because poor predictions may lead to inappropriate decisions, putting critical resources at risk, particularly in coastal environments.

The Assessment of an Effect of Natural Origin Products on the Initial Growth and Development of Maize under Drought Stress and the Occurrence of Selected Pathogens

Poland, like other countries in the world, increasingly experiences the ongoing climate change that is a critical yield-limiting factor. The use of biostimulants in agriculture has shown tremendous potential in combating climate change-induced stresses such as drought, temperature stress, etc. They could be a promising tool in the current crop production scenario. Biostimulants are organic compounds, microbes, or amalgamation of both that could regulate plant growth behavior through molecular alteration and physiological, biochemical, and anatomical modulations. They can promote plant growth under various environmental stresses because they have a positive effect, in particular, on plant growth and resistance. There are many products of this type available on the market, including those of natural origin, which are part of the Integrated Pest Management. The ecotoxicity of chemical plant protection products, the negative effects of their use, and the change in regulations make it recommended to use low-risk chemicals and non-chemical methods, that involve the least risk to health and the environment, and at the same time ensure effective and efficient protection of crops. Natural origin biocomponents obtained by the supercritical CO2 extraction of plant material or by fermentation process in bioreactors were tested. Common maize (Zea mays L.) was selected as a test plant for growth tests at climate chambers. Results showed that the only supernatant (fermentation broth) obtained with the Paenibacillus bacteria (S2) had a positive effect on the germination index (GI > 100%) of maize seeds, compared to the obtained plant seed extracts from the crop of the legume family (Fabaceae) (E3) and from the crop of the smartweed family (Polygonaceae) (E9) (GI < 100%). The extracts E3, S1 (supernatant obtained with the use of bacteria from the genus Enterobacter) and S2 used as a single product and in combination with UAN+S, under optimal conditions of the experiment, had a positive effect on the maize root weight compared to the untreated, while under drought stress, a decrease in the root weight was observed. Moreover, on the basis of the conducted research, differences in the mycelial growth of selected fungi were found. The applied biocomponent S2 of microbial origin extract (supernatant 2) showed a mycelial growth-limiting effect on all tested Fusarium fungi isolated from the corn cobs.

Electricity supply and demand dynamics in Iran considering climate change-induced stresses

The impacts of climate change such as water scarcity and air temperature increase affect the development of the electricity supply system. In countries like Iran, where cooling demand in the residential sector is high and water availability is fairly low, this can create significant challenges. In this study, an econometric approach was used to project the electricity demand for four sectors in Iran, considering the effect of changing air temperature. Also, the impact of water scarcity on the optimal expansion of the power system was investigated using TIMES, a bottom-up energy system optimization modeling platform, to build a model for the Iranian electricity system with each power plant unit modeled separately. The option of switching cooling technology was also included in the modeling. The results show that a fuel price increase alters the optimal capacity and cooling technology portfolio substantially. Renewables become more desirable under the scenarios in which the fuels and water prices, as well as the carbon taxes, are high. The emissions increase in all scenarios, although the carbon tax moderates this increase. The water withdrawal declines in the high water price scenarios. The sensitivity analysis of water price revealed the effect of this parameter on water withdrawal.

Role of biostimulants in mitigating the effects of climate change on crop performance.

Climate change is a critical yield-limiting factor that has threatened the entire global crop production system in the present scenario. The use of biostimulants in agriculture has shown tremendous potential in combating climate change-induced stresses such as drought, salinity, temperature stress, etc. Biostimulants are organic compounds, microbes, or amalgamation of both that could regulate plant growth behavior through molecular alteration and physiological, biochemical, and anatomical modulations. Their nature is diverse due to the varying composition of bioactive compounds, and they function through various modes of action. To generate a successful biostimulatory action on crops under different parameters, a multi-omics approach would be beneficial to identify or predict its outcome comprehensively. The 'omics' approach has greatly helped us to understand the mode of action of biostimulants on plants at cellular levels. Biostimulants acting as a messenger in signal transduction resembling phytohormones and other chemical compounds and their cross-talk in various abiotic stresses help us design future crop management under changing climate, thus, sustaining food security with finite natural resources. This review article elucidates the strategic potential and prospects of biostimulants in mitigating the adverse impacts of harsh environmental conditions on plants.

English

A desk review, survey, interview and on-site observation were used to collect data. The impact of external systems on smallholder water scarcity management institutions in the context drought stress was analyzed in this study. The findings show that the external intervention eliminated resilient people’s institutions such as water scarcity (in droughts). Interventions introduce new water management systems that fuel tension, conflict and water poverty. The local water management systems are sustainable and enhance social cohesion and trust while the new program-based institutional arrangements/systems are nepotic and corrupt in terms of ensuring equality and addressing needs of diverse beneficiaries. A local peoples’ institutional approach is recommended to respond to climate change-induced stresses and such approach is capable of managing social, political, economic and environmental dimensions. Policy interventions need to consider a bottom-up approach to accommodate local specific context as well as built on existing local systems that ensure social cohesion among members of community. Interventions that meet pro-poor, inclusive and sustainable objectives need to adopt a human-rights based transformative approaches and such an approach inclusive and sustainable. Key words: Institutional conflict, drought stress, pro-poor policy.

Auxiliary Seed Treatment Is Necessary to Increase Recruitment of a Critically Endangered Species, Abies beshanzuensis (Pinaceae)

Disordered germination is widely observed in plant species with extremely small populations (PSESPs) in China. Abies beshanzuensis M. H. Wu, a PSESP (with only three extant adults in Zhejiang Province, China) that also has poor seed germination in the field, belongs to the Pinaceae family. Pinaceae generally tend toward symbiosis with ectomycorrhizal (ECM) fungi to alleviate climate change-induced stresses. Therefore, exploring how to improve seed germination of A. beshanzuensis and whether it is an ECM species will contribute to increasing recruitment for conducting further conservation research. In this study, four temperature regimes (10/15 °C, 15/20 °C, 20/25 °C, and 25/30 °C) were selected to explore the response of seed germination to rising temperature. Based on the microscopic features of fine roots, together with molecular techniques, the mycorrhizal type of this species was identified. The seed germination of A. beshanzuensis was increased from 1–2% to around 4.5% by 14-day cold stratification and under 20/25 °C fluctuating temperature conditions. Our findings indicated that A. beshanzuensis may be endangered as a result of insufficient seedling recruitment due to poor germination under the current climate. A. beshanzuensis was confirmed as an ECM fungi-associated tree species. This study highlights the necessity of incorporating auxiliary seed treatment into population recovery programs of A. beshanzuensis, thereby better conserving the species under ongoing environmental changes.

Copepod reproductive effort and oxidative status as responses to warming in the marine environment.

The marine ecosystems are under severe climate change‐induced stress globally. The Baltic Sea is especially vulnerable to ongoing changes, such as warming. The aim of this study was to measure eco‐physiological responses of a key copepod species to elevated temperature in an experiment, and by collecting field samples in the western Gulf of Finland. The potential trade‐off between reproductive output and oxidative balance in copepods during thermal stress was studied by incubating female Acartia sp. for reproduction rate and oxidative stress measurements in ambient and elevated temperatures. Our field observations show that the glutathione cycle had a clear response in increasing stress and possibly had an important role in preventing oxidative damage: Lipid peroxidation and ratio of reduced and oxidized glutathione were negatively correlated throughout the study. Moreover, glutathione‐s‐transferase activated in late July when the sea water temperature was exceptionally high and Acartia sp. experienced high oxidative stress. The combined effect of a heatwave, increased cyanobacteria, and decreased dinoflagellate abundance may have caused larger variability in reproductive output in the field. An increase of 7°C had a negative effect on egg production rate in the experiment. However, the effect on reproduction was relatively small, implying that Acartia sp. can tolerate warming at least within the temperature range of 9–16°C. However, our data from the experiment suggest a link between reproductive success and oxidative stress during warming, shown as a significant combined effect of temperature and catalase on egg production rate.

Challenges and Perspectives in the Epigenetics of Climate Change-Induced Forests Decline.

Forest tree species are highly vulnerable to the effects of climate change. As sessile organisms with long generation times, their adaptation to a local changing environment may rely on epigenetic modifications when allele frequencies are not able to shift fast enough. However, the current lack of knowledge on this field is remarkable, due to many challenges that researchers face when studying this issue. Huge genome sizes, absence of reference genomes and annotation, and having to analyze huge amounts of data are among these difficulties, which limit the current ability to understand how climate change drives tree species epigenetic modifications. In spite of this challenging framework, some insights on the relationships among climate change-induced stress and epigenomics are coming. Advances in DNA sequencing technologies and an increasing number of studies dealing with this topic must boost our knowledge on tree adaptive capacity to changing environmental conditions. Here, we discuss challenges and perspectives in the epigenetics of climate change-induced forests decline, aiming to provide a general overview of the state of the art.

Micro-level evaluation of socio-technological interventions to address climate change-induced stresses in dairy enterprises

Micro-level evaluation of socio-technological interventions to address climate change-induced stresses in dairy enterprises

Drivers of declines in common loon (Gavia immer) productivity in Ontario, Canada

Common loons (Gavia immer) are top predators that depend on lake food webs to successfully fledge chicks. Common loon reproductive success is consequently recognized as an important indicator of aquatic ecosystem health. Existing evidence points to long-term declines in productivity in portions of the common loon range; however, the reason for these declines is not well understood. Our objectives were to define underlying baseline patterns of loon reproductive success in Ontario, Canada, and to identify drivers of temporal changes in loon productivity. We analyzed 38 years of reproductive data from over 1500 lakes using data from the Canadian Lakes Loon Survey, a citizen science loon monitoring program managed by Birds Canada that has run annually in Ontario since 1981. Overall, we estimated a declining trend in common loon reproductive success of −0.10 six-week-old young per pair per year in Ontario between 1981 and 2018. We assessed the influence of 14 factors on loon reproductive success. We identified low pH and associated higher mercury as factors linked to loon productivity declines. We also demonstrated that lake area, longitude, and April temperatures can predict the number of six-week-old young per pair per year. We hypothesize that climate change-induced stress, acting through multiple interacting pathways involving mercury acidity, fish abundance, lake size, and geographic location, may account for declining loon productivity. These results will be important for focusing future research and conservation efforts to help understand and mitigate threats to common loon populations.

Specificity trumps flexibility-location-based stable associations between Symbiodiniaceae genera and Platygyra verweyi (Scleractinia; Merulinidae).

This study monitored symbiont communities bi-monthly in native coral cores used in a reciprocal transplantation of the coral Platygyra verweyi over two years (2014–2016) and samples of mother colonies from three locations with variable thermal regimes; our results show that associating with multiple Symbiodiniaceae genera (Cladocopium spp. and Durusdinium spp.) is not a prerequisite for symbiont shuffling. Platygyra verweyi associates with certain Symbiodiniaceae genera based on location. Results of quantitative real-time PCR indicated small-scale temporal changes in Symbiodiniaceae genera compositions from 2014 to 2016; however, these changes were not enough to invoke shuffling or switching, despite degree heating weeks exceeding 6 °C-weeks in 2014 and 4 °C-weeks in 2015, which usually resulted in substantial coral bleaching. Microsatellite analysis of the P. verweyi host showed no genetic differences among the study locations. Our results suggest that P. verweyi undergoes long-term acclimatization and/or adaptation based on microgeographic and local environmental conditionsby altering its combinations of associated Symbiodiniaceae. Results also suggest that shuffling might not be as common a phenomenon as it has been given credit for; corals thrive through specific associations, and many corals could still be vulnerable to climate change-induced stress, despite being promiscuous or able to associate with rare and background Symbiodiniaceae genera.