Clerodendrum Trichotomum Research Articles

Clerodendrum trichotomum Extract Attenuates UV-B-Induced Skin Impairment in Hairless Mice by Inhibiting MAPK Signaling.

Exposure to solar ultraviolet-B (UV-B) radiation significantly accelerates skin aging by inducing the expression of matrix metalloproteinases (MMPs) such as MMP-1, leading to alterations in the extracellular matrix and consequent photoaging. Some plant components, renowned for their UV-absorbing and antioxidative properties, show potential for mitigating photoaging by reducing UV-B-induced MMP levels. In this context, we explored the inhibitory effects of Clerodendrum trichotomum extract (CTE) on UV-B-induced skin damage. The mechanism of CTE was predicted using network pharmacology approach. Also, antiaging efficacy was evaluated by mouse model and cellular system using human epidermal keratinocytes (HEKa), including its modulation of mitogen-activated protein kinase (MAPK) signaling pathways. CTE effectively counters UV-B-induced skin damage, as evidenced by the suppression of MMP-9 and MMP-1 expression in mice. We found that each fraction and chemical constituents of CTE suppressed UV-B-induced MMP-1 secretion in HEKa cells. CTE inhibits UV-B-induced skin aging by partially suppressing MMP-1 and MMP-9 secretion via the modulation of MAPK signaling pathways.

Bioactive constituents from Clerodendrum trichotomum and their α-glucosidase inhibitory and PPAR-γ agonist activities

Three new neoclerodane diterpenoids (1–3), two new steroids (4–5), one new monoterpene (6), one new derivative of benzaldehyde (7) and one new iridoid glycoside (8), along with 19 known phenolic compounds, were isolated from Clerodendrum trichotomum. Their structures were established by a combination of detailed spectroscopic analyses (1D and 2D NMR) and high resolution electrospray ionization mass spectroscopy (HRESIMS). The isolated compounds were screened on α-glucosidase inhibitory and the peroxisome proliferator-activated receptor gamma (PPAR-γ) agonist activities, and the results showed that three phenylethanoid glycosides, verbascoside (9), leucosceptoside a (10), and isoacteoside (13), and two flavonoids, apigenin (22) and luteolin (26) showed potent inhibitory effects against α-glucosidase, with IC50 values in the range from 15 to 700 μM. In addition, four flavonoids apigenin 7-O-β-D-glucuronide (19), apigenin (22), luteolin (26), and quercetin (27) exhibited significant PPAR-γ agonistic activities with EC50 values in the range 2.3–24.9 μM.

Clerodendrum trichotomum Thunberg-An Ornamental Shrub with Medical Properties.

Harlequin glory bower (Clerodendrum trichotomum) is a shrub or small tree belonging to the Lamiaceae family, native to Japan, Korea, and eastern China. It has esthetic value and in Europe, it is cultivated as an ornamental plant. Its sweet-smelling flowers have a white or pink crown. The calyx turns from green to pink-purple over time, providing an especially decorative touch around surrounding the ripe deep-blue fruits that persist until winter. In the areas of its natural occurrence, the leaves and young shoots of C. trichotomum, and sometimes the roots, flowers and fruits, are used in folk medicine due to its anti-inflammatory, analgesic, anticancer, sedative, and hypotensive effects. Products based on Harlequin glory are also used in the treatment of rheumatoid arthritis, joint pain, skin inflammation, or asthma. Preliminary research on the composition of raw material suggests that its health-promoting effect is associated with the presence of numerous secondary metabolites, including phenylpropanoids, flavonoids, lignans, terpenoids, steroids, alkaloids, and anthraquinones. This work reviews the current state of knowledge about the phytochemistry and in vitro and in vivo pharmacological properties of the extracts and main active components isolated from C. trichotomum. It also indicates that before it can be used in modern medicine, further research is necessary regarding the safety and efficacy of the raw material, its mechanisms of action, and dosage.

New floristic records from Central Europe 13 (reports 176-197)

The presented 13th part of the series includes 22 new records of vascular plants from the territory of Central Europe. In Hungary, 5 species, Clerodendrum trichotomum, Morus indica, Oenanthe banatica, Rumex confertus and Torilis nodosa are mentioned. Single findings of alien Panicum virgatum in Poland as well as Euphorbia lathyris in Romania were recorded. In Slovakia, twelve species, Agrostemma githago, Carex hordeistichos, Camelina sativa subsp. zingeri, Consolida hispanica, Geranium lucidum, Lobularia maritima, Ranunculus pedatus, Reseda phyteuma, Rubus radula, Sagittaria latifolia, Sedum pallidum and Trifolium strictum were recorded. From the Ukrainian Carpathians 3 new records of Euphrasia minima subsp. tatrae, Neotinea ustulata and Salix herbacea were reported.

Analysis of phytochemical, antioxidant, and α-amylase inhibition capacity of methanol extract of six plants from Kaski, Gulmi and Rupandehi districts of Nepal

This study was designed to explore phytochemicals and biological activities of methanolic extract of six traditional medicinal plants (Clerodendrum trichotomum, Mallotus philipinensis, Dioscorea-bublifera, Rubia cordifolia, Viscum articulatum) of Nepalese origin. Plant extracts were prepared in methanol through cold percolation. Analysis of phytochemical constituent, antioxidant capacities and brine shrimp lethality assay of the test plant samples were carried out using standard methods. The 1,1-diphenyl-2-picryl hydrazyl (DPPH) method was used to study antioxidant activity of different plant extracts. Furthermore, starch-iodine method was used to study the inhibition effect of extract on α-amylase. Phytochemical analysis showed the presence of phytochemicals like alkaloids, flavonoids, phenolic content, glycosides, reducing sugars, etc. in six medicinal plants. Brine shrimp lethality assay suggested the presence of pharmacologically active compounds. Total phenolic content and total flavonoid content of C. trichotomum’s leaves extract were found to be higher with 212.742 mg GAE/g and 112 mg Q/g respectively with strong antioxidant activity. Similarly, the α-amylase inhibition of R. cardifolia’s root extract and C. trichotomum‘s leaves extract was found to be 252.44±0.55 µg/mL and 293.33± 0.81 µg/mL comparative with IC50 value 119.063±0.73 µg/mL of standard acarbose that showed remarkable antidiabetic property among six samples. The results, obtained here suggested that six medicinal plants i.e. C. trichotomum, M. philipinensis, D. deltoidea, D. bublifera, R. cordifolia, and V. articulatum, Nepal origin showed biological activity by targeting multiple drug targets which justifies their traditional uses. This is the first finding that C. trichotomum‘s leaves can be a promising source for the development of natural antioxidant and antidiabetic agents.

Two New Abietane Diterpenes From the Stems of Clerodendrum trichotomum Thunb

Two new abietane diterpenoids (4 S, 5 S, 10 S)-12-( β-D-glucopyranosyl)oxy-11-hydroxyabieta-8,11,13-triene-19-oic acid β-D-glucopyranosyl-(1→6)- β-D-glucopyranosyl ester (1) and (3 S, 5 S, 10 S, 15 S)-3 β-[ β-D-glucopyranosyl-(1→6)- β-D-glucopyranosyl]oxy-12-( β-D-glucopyranosyl)oxyabieta-8,11,13-triene-11,16-diol (2), along with 5 known terpene glycosides (3–7) were isolated from the n-butanol extract of the stems of Clerodendrum trichotomum Thunb. The structures of the new compounds were determined by analysis of spectroscopic data, chemical correlations, and electronic circular dichroism calculations. Besides, all compounds were evaluated for cytotoxic activities against cultured K562, MCF-7, A549, and HepG2 cell lines. None of them showed good antitumor activities.

Clerodendrum trichotomum extract improves metabolic derangements in high fructose diet-fed rats

ABSTRACT Clerodendrum trichotomum has been reported to possess beneficial properties for human health, but its effects on metabolic syndrome have not been reported. In this study, we investigated the effect of C. trichotomum leaf extract (CT) on the metabolic derangements induced by a high-fructose (HF) diet. Sprague–Dawley rats were fed with a 46% carbohydrate diet (HC group), 60% high-fructose diet (HF group), or HF diet supplemented with CT (500 mg/kg of body weight/day, CT group) via drinking water for 16 weeks. Results showed that CT alleviated HF diet-induced insulin resistance, dyslipidemia, and hepatic steatosis In liver tissues, CT affected the signaling pathways of AMP-activated protein kinase, peroxisome proliferator-activated receptor α (PPARα), and sterol regulatory element binding protein 1. CT enriched the genes that were mainly involved in cytokine-cytokine receptor interaction, PPAR, PI3K-Akt signaling pathways, and fatty acid metabolism pathway. These results suggest that CT is a promising therapeutic against metabolic disorders.

Two novel polyketones from the leaves and twigs of Clerodendrum trichotomum

Two novel polyketones, along with four known compounds, were isolated from the leaves and twigs of Clerodendrum trichotomum. Their structures were determined to be clerodendruketone A (1), clerodendruketone B (2), ecdysanols E (3), ecdysanols D (4), 5,5′-dimethoxy-7-oxolariciresinol (5), and (-)-(7′S,8S,8′R)-4,4′-dihydroxy-3,3′,5,5′-tetramethoxy-7′,9-epoxy-lignan-9′-ol-7-one (6) through the methods of NMR, HRESIMS and ECD data analyses. The antioxidant effects against free radical were tested by DPPH assay. The antibacterial activity against Escherichia coli and Staphylococcus aureus were tested by turbidimetry assay. The results demonstrated that compounds 1 and 2 had significative antibacterial activity, and compound 3 had moderate antioxidant activity.

Relationship between interspecific pollen transfer and pistil length in sympatric congeners, Clerodendrum trichotomum and C. izuinsulare

AbstractInterspecific pollen transfer (IPT) often leads to reproductive interference. Although character displacement of reproductive traits in plants is often considered a consequence of reproductive interference, few studies have tested whether intraspecific variation in floral morphology changes the intensity of reproductive interference among pollinator‐sharing plants. We investigated whether intraspecific variation in pistil length changes the proportion of IPT (interspecific pollen transfer) in sympatric populations of Clerodendrum trichotomum and C. izuinsulare on the two islands. On Toshima Island, C. izuinsulare flowers with shorter pistils were significantly less likely to receive C. trichotomum pollen, and there was a slight tendency for longer pistiles of C. trichotomum flowers to receive less C. izuinsulare pollen, suggesting that IPT has caused character displacement in pistil length in these congeneric plants. In contrast, we did not detect any relationship between pistil length and the proportion of IPT for the two species on Niijima Island. The discrepancy between the islands may reflect differences in pollinator assemblages.

명승 부안 채석강ㆍ적벽강 일원의 경관관리를 위한 관속식물상 연구

This research was conducted to provide basic information for landscape management, natural resource 􀁆􀁒􀁑􀁖􀁈􀁕􀁙􀁄􀁗􀁌􀁒􀁑􀀃􀁄􀁑􀁇􀀃􀁘􀁗􀁌􀁏􀁌􀁝􀁄􀁗􀁌􀁒􀁑􀀃􀁗􀁋􀁕􀁒􀁘􀁊􀁋􀀃􀁖􀁜􀁖􀁗􀁈􀁐􀁄􀁗􀁌􀁆􀀃􀁕􀁈􀁖􀁈􀁄􀁕􀁆􀁋􀀃􀁒􀁑􀀃􀃀􀁒􀁕􀁄􀀃􀁄􀁑􀁇􀀃􀁏􀁄􀁑􀁇􀁖􀁆􀁄􀁓􀁈􀀃􀁆􀁋􀁄􀁑􀁊􀁈􀁖􀀃􀁒􀁉􀀃􀀶􀁆􀁈􀁑􀁌􀁆􀀃􀀶􀁌􀁗􀁈􀀃 (No. 13) Chaeseokgang and Jeokbyeokgang Cliffed Coasts, Buan. A total of 183 taxa of vascular plants were identified, the detail being 65 families, 138 genus, 157 species, 1 subspecies, 23 varieties, and 2 forms. There were only two ferns and two gymnosperms, but also 175 types of angiosperms were 􀁌􀁇􀁈􀁑􀁗􀁌􀂿􀁈􀁇􀀏􀀃􀁚􀁋􀁌􀁆􀁋􀀃􀁌􀁖􀀃􀀜􀀘􀀑􀀙􀀈􀀃􀁒􀁉􀀃􀁗􀁋􀁈􀀃􀁗􀁒􀁗􀁄􀁏􀀑􀀃􀀤􀁐􀁒􀁑􀁊􀀃􀁗􀁋􀁈􀀃􀁓􀁏􀁄􀁑􀁗􀁖􀀃􀁕􀁈􀁖􀁈􀁄􀁕􀁆􀁋􀁈􀁇􀀏􀀃􀁗􀁋􀁈􀀃􀁗􀁄􀁛􀁒􀁑􀀃􀁚􀁌􀁗􀁋􀀃􀁗􀁋􀁈􀀃􀁊􀁕􀁈􀁄􀁗􀁈􀁖􀁗􀀃􀁑􀁘􀁐􀁅􀁈􀁕􀀃 􀁚􀁄􀁖􀀃􀀦􀁒􀁐􀁓􀁒􀁖􀁌􀁗􀁄􀁈􀀃􀁚􀁋􀁌􀁆􀁋􀀃􀁕􀁈􀁆􀁒􀁕􀁇􀁈􀁇􀀃􀀕􀀙􀀃􀁗􀁜􀁓􀁈􀁖􀀑􀀃􀀤􀀃􀁗􀁒􀁗􀁄􀁏􀀃􀁒􀁉􀀃􀀗􀀃􀁗􀁄􀁛􀁄􀀃􀁒􀁉􀀃􀁈􀁑􀁇􀁈􀁐􀁌􀁆􀀃􀁓􀁏􀁄􀁑􀁗􀁖􀀃􀁚􀁈􀁕􀁈􀀃􀁌􀁇􀁈􀁑􀁗􀁌􀂿􀁈􀁇􀀞􀀃􀀦􀁏􀁈􀁐􀁄􀁗􀁌􀁖􀀃 brachyura, Populus tomentiglandulosa, Broussonetia kazinoki Siebold, and Saussurea eriophylla Nakai, while two taxa of endangered plants were identified; Koelreuteria paniculata and Glehnia littoralis. There were a total of 10 taxa in case of naturalized plants, including Brassica campestris subsp. napus var. nippo-oleifera Makino, Lepidium virginicum L., Chenopodium ficifolium Sm., and Phytolacca americana L., with the naturalization rate being 5.5%. According to the analysis of naturalized degrees of the naturalized plants, there were 7 plants with 5th degree of naturalization while there were one species for each 4th, 3rd, and 2nd degrees, and there were no 1st degree plant. The vegetation in the Gyeokpo-ri area is divided into 13 plant communities. The representative plant colonies include Pinus thunbergii colony, Pinus thumbergii-Quercus variabilis colony, Pinus thunbergii-Quercus acutissima colony, Carpinus turczaninovii Hance-Pinus thunbergii colony, Carpinus turczaninovii Hance colony, and Clerodendrum trichotomum -Euonymus japonicus colony, with Pinus thunbergii colony taking up 53.55% of the total. As the vegetation in Scenic Site Chaeseokgang and Jeokbyeokgang Cliffed Coasts, Buan mainly consist of Pinus thunbergii colony and mixed pinus thunbergii forest, the vegetation landscape does not become different throughout four seasons, and one can feel partial seasonal changes because deciduous trees, such as Quercus species and Carpinus turczaninovii Hance, are spread in parts.

Prevention of Hyperuricemia by Clerodendrum trichotomum Leaf Extract in Potassium Oxonate-Induced Mice.

Clerodendrum trichotomum is a folk medicine exhibiting anti-hypertension, anti-arthritis, and anti-rheumatism properties. However, little is known about whether the material might prevent hyperuricemia and associated inflammation. In this study, we explored whether C. trichotomum leaf extract (CTE) prevented hyperuricemia induced by potassium oxonate (PO) in mice. CTE (400 mg/kg body weight) significantly reduced the serum uric acid (UA), blood urea nitrogen (BUN), and serum creatinine levels and increased urine UA and creatinine levels. CTE ameliorated PO-induced inflammation and apoptosis by reducing the levels of relevant proteins in kidney tissues. Also, CTE ameliorated both UA-induced inflammatory response in RAW 263.7 cells and UA-induced cytotoxicity in HK-2 cells. In addition, liver transcriptome analysis showed that CTE enriched mainly the genes for mediating positive regulation of MAPK cascade and apoptotic signaling pathways. Together, the results show that CTE effectively prevents hyperuricemia and associated inflammation in PO-induced mice.

Pyrolysis molecule of Clerodendrum trichotomum for potential biomedicine

In this experiment, Fourier transform infrared technology, gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry were used to detect and analyze the extract of Clerodendrum trichotomum root. The results showed that the extract of Clerodendrum trichotomum root contained a large amount of biologically active ingredients and active ingredients. Clerodendrum trichotomum is rich in essential ingredients and has broad application prospects in biopharmaceuticals and fragrances. This study is of great significance for the development of the medicinal potential of Clerodendrum trichotomum and the identification of its value for further development in modern medicine.

SSR marker development in Clerodendrum trichotomum using transcriptome sequencing.

Clerodendrum trichotomum, a member of the Lamiaceae (Verbenaceae) family, is an ornamental plant widely distributed in South Asia. Previous studies have focused primarily on its growth characteristics, stress resistance, and pharmacological applications; however, molecular investigations remain limited. Considering germplasm conservation and the extensive applications of this plant, it is necessary to explore transcriptome resources and SSR makers for C. trichotomum. In the present study, RNA sequencing was used to determine the transcriptome of C. trichotomum. Subsequently, unigene annotations and classifications were obtained, and SSRs were mined with MIcroSAtellite. Finally, primer pairs designed with Oligo 6.0 were selected for polymorphism validation. In total, 127,325,666 high-quality reads were obtained, and 58,345 non-redundant unigenes were generated, of which 36,900 (63.24%) were annotated. Among the annotated unigenes, 35,980 (97.51%) had significant similarity to 607 species in Nr databases. In addition, a total of 6,444 SSRs were identified in 5,530 unigenes, and 200 random primer pairs were designed for polymorphism validation. Furthermore, after primary polymorphism identification, 30 polymorphic primer pairs were selected for the further polymorphism screening, and 200 alleles were identified, 197 of which showed polymorphism. In this work, a large number of unigenes were generated, and numerous SSRs were detected. These findings should be beneficial for further investigations into germplasm conservation and various applications of C. trichotomum. These results should also provide a solid foundation for future molecular biology studies in C. trichotomum.

Anti-Respiratory Syncytial Virus Activity of Plantago asiatica and Clerodendrum trichotomum Extracts In Vitro and In Vivo.

The herbs Plantago asiatica and Clerodendrum trichotomum have been commonly used for centuries in indigenous and folk medicine in tropical and subtropical regions of the world. In this study, we show that extracts from these herbs have antiviral effects against the respiratory syncytial virus (RSV) in vitro cell cultures and an in vivo mouse model. Treatment of HEp2 cells and A549 cells with a non-cytotoxic concentration of Plantago asiatica or Clerodendrum trichotomum extract significantly reduced RSV replication, RSV-induced cell death, RSV gene transcription, RSV protein synthesis, and also blocked syncytia formation. Interestingly, oral inoculation with each herb extract significantly improved viral clearance in the lungs of BALB/c mice. Based on reported information and a high-performance liquid chromatography (HPLC) analysis, the phenolic glycoside acteoside was identified as an active chemical component of both herb extracts. An effective dose of acteoside exhibited similar antiviral effects as each herb extract against RSV in vitro and in vivo. Collectively, these results suggest that extracts of Plantago asiatica and Clerodendrum trichotomum could provide a potent natural source of an antiviral drug candidate against RSV infection.

Abietane Diterpenoids from the Roots of Clerodendrum trichotomum and Their Nitric Oxide Inhibitory Activities.

Twelve new abietane diterpenoids (1-12) and 31 known analogues (13-43) were isolated from a medicinal Chinese herb, Clerodendrum trichotomum Thunberg. The absolute configurations of 1-3 were established on the basis of ECD and X-ray crystallography data, whereas that of 4 was elucidated by comparison of experimental and calculated ECD data. Eight diterpenoids, 15,16-dehydroteuvincenone G (1), trichotomin A (4), 2α-hydrocaryopincaolide F (7), villosin C (20), 15-dehydro-17-hydroxycyrtophyllone A (22), demethylcryptojaponol (38), 6β-hydroxydemethylcryptojaponol (39), and trichotomone (43), exerted inhibitory effects against NO production with IC50 values of 5.6-16.1 μM. The structure-activity relationships of the isolated diterpenoids are also estimated.

Physiological Activity of Clerodendrum trichotomum Ethanol Extracts for the Development of Materials for Cosmetics

Physiological Activity of Clerodendrum trichotomum Ethanol Extracts for the Development of Materials for Cosmetics

A Study on Cytotoxicity and Antioxidant Activity of Clerodendrum Trichotomum Extract

In this study, an antioxidant activity, anti-inflammatory and antibacterial experiment was conducted to develop natural cosmetic materials depending on biological activity and cytotoxicity of the Clerodendrum trichotomum extract and the results were as follows. The content of polyphenol was 119.723±1.0㎎/g and when different concentrations of the extract were applied, DPPH radical scavenging activity was measured at 3.2, 4.0, 13.5, 24.7, 77.1, and 78.1% respectively. This means that the higher the concentration, the higher antioxidant activity. For antibacterial measurement, S. aureus, S. epidermidis, E. coli, and P. acnes were added. As a result, S. aureus and P. acnes had the highest value(16㎜) at a concentration of 1㎎/㎖, S. aureus had the highest value(16㎜) at a concentration of 5㎎/㎖, and S. epidermidis had the highest value(15㎜) at a concentration of 10㎎/㎖. Also, S. aureus and S. epidermidis had the highest value(16㎜) at a concentration of 20㎎/㎖. ABTS radical scavenging activity was measured at 0.645, 4.677, 15.054, 31.774, 101.237, and 101.022% respectively, when different concentrations of the extract were applied. The result was greater than or similar to Vit and C, which are standard controls, at a concentration of 500 and 1000㎍/㎖. The cell survival rate was 92.738, 84.260, 83.964, 86.987, 86.589, and 70.497% and there was no cytotoxicity of the extract. The expression of iNOS declined when th concentration of the extract was 500㎍/㎖ and in the COX-2 experiment, RAW 264.7 using the pre-treated extract failed to inhibit COX-2 that increased due to LPS. In conclusion, it is considered that the findings of this study can be used as important basic data to develop natural cosmetic materials using the Clerodendrum trichotomum extract.

Anti-oxidant and Anti-inflammatory Properties of Clerodendrum trichotomum Leaf Extracts

Anti-oxidant and Anti-inflammatory Properties of Clerodendrum trichotomum Leaf Extracts

Natural distribution and community ecological characteristics of Taxus chinensis var. mairei in Shanxi Province, China.

Taxus chinensis var. mairei is a national Category 1 protective species in China, with its northernmost natural distribution to the southeast of Shanxi Province, China. This study was conducted to investigate the natural distribution of T. chinensis var. mairei in Shanxi Province. The community classification, species diversity, population structure and competition intensity of T. chinensis var. mairei were studied. The results showed that T. chinensis var. mairei mainly formed four community types, including the community of T. chinensis var. mairei + Carpinus turczaninowii, the community of T. chinensis var. mairei - Vitex negundo var. heterophylla, the community T. chinensis var. mairei - Clerodendrum trichotomum and the community of T. chinensis var. mairei + Quercus variabilis. T. chinensis var. mairei was concentratedly distributed in Mohe of Linchuan County and Manghe Nature Reserve of Yangcheng County, and scattered in some other areas. The communities had an obviously vertical structure, with T. chinensis var. mairei trees growing into main canopy which were about 8-10 m high in mixed forests and 5-6 m high in pure forests. The species diversity index and evenness index had significant difference among the four community types. The average height of T. chinensis var. mairei was 5.16 m. The individuals in tree layer and succession layer were 43.4% and 56.6%, respectively, while they were scarce in the regeneration layer. 67.6% of the trees were small-diameter (DBH＜16 cm) and only 4.0% were large-diameter (32 cm≤DBH＜40 cm). T. chinensis var. mairei was considered to be lack of seedlings because only 4 seedlings were found, which might result in the declination of this species in future. T. chinensis var. mairei in Shanxi Province showed relatively concentrated distribution, with most individuals being small-diameter, resulting in tense intraspecific competition which accounted for 61.8% of the total intensity. The interspecific competition to T. chinensis var. mairei was mainly from C. turczaninowii and Q. variabilis, which were two dominant tree species with larger population in this area.

Scientific validation of medicinal plants used by Yakkha community of Chanuwa VDC, Dhankuta, Nepal.

Ethnobotanical knowledge is important among tribal people, but much of the information is empirical due to the lack of scientific validation. The purpose of this study was to document the medicinal plants used by an ethnic group (Yakkha) at Chanuwa VDC of Dhankuta district in Nepal and to validate scientifically in the use of plants based on results of phytochemical, antimicrobial and antioxidant property analyses and available literature reports. Data were collected through interviews of the Yakkha people with the help of a semi-structured questionnaire and the guided field walk method. A total of 30 different medicinal plants were recorded along with their vernacular names (for few plants) used by the Yakkha community’s people. Literature review reveals that most of the plant species described herein have also been used in other countries, too. Among 30 plants selected for this study methanol extract of five ethno-medicinal plants viz., Dendrocnide sinuata, Solanum anguivi, Pogostemon cablin, Boehmeria platyphylla and Clerodendrum trichotomum and ethanol extract of C. trichotomum were subjected for antibacterial and antioxidant properties. The antimicrobial activities were measured using the paper disc diffusion method. The antioxidant properties of plants were measured by DPPH and FRAP reduction assay. Among all extracts, ethanol extract of C. trichotomum and methanol extract of B. platyphylla displayed the highest antibacterial and antioxidant activities, respectively.