Clear Daily Rhythm Research Articles

Daily and other short-term changes in the ecosystem components of the world's largest hypersaline lagoon Bay Sivash (Crimea)

The interrelationship between various processes and components in the daily cycle of water bodies has been little studied, especially in hypersaline water bodies. A three-day study was conducted in the world's largest hypersaline lagoon Bay Sivash (the Sea of Azov). 16 biotic and abiotic parameters were measured 21–80 times in the studied site (wind direction and speed, photosynthetically active radiation (PAR), temperature, oxygen content, pH, Eh, turbidity, chlorophyll a fluorescence, zooplankton abundance, and others). There was a clear daily rhythm of PAR in the surface layer of the water, however, the stochastic weather factor (cloudiness, wind speed and direction) influenced the quantitative expression of it. The proportion of PAR entering deeper layers as shown, depends on the height of the Sun, as well as intra-ecosystem factors, for example turbidity and the development of mats of filamentous algae. Fluctuating from 1.69 to 98.03 NTU, turbidity depended on the speed and direction of the wind with windward winds leading to the resuspension of bottom sediments to a greater extent than leeward ones. Wind speed and direction also significantly influenced the distribution of biotic components such as the filamentous green alga Cladophora and zooplankton. Oxygen content fluctuated from between 1.8 and 16.58 mg L−1. An increase in oxygen concentration was observed only at PAR above approximately 500–1000 μE m−2;s−1. The increase in temperature and the virtual absence of wind, which was observed on 05/28/2022 and 05/29/2022, contributed to an increase in the oxygen concentration in the water on these days and the formation of hyperoxic conditions in this hypersaline lagoon.

Energetic Demands Regulate Sleep-Wake Rhythm Circuit Development.

Sleep and feeding patterns lack strong daily rhythms during early life. As diurnal animals mature, feeding is consolidated to the day and sleep to the night. In Drosophila, circadian sleep patterns are initiated with formation of a circuit connecting the central clock to arousal output neurons; emergence of circadian sleep also enables long-term memory (LTM). However, the cues that trigger the development of this clock-arousal circuit are unknown. Here, we identify a role for nutritional status in driving sleep-wake rhythm development in Drosophila larvae. We find that in the 2nd instar larval period (L2), sleep and feeding are spread across the day; these behaviors become organized into daily patterns by the 3rd instar larval stage (L3). Forcing mature (L3) animals to adopt immature (L2) feeding strategies disrupts sleep-wake rhythms and the ability to exhibit LTM. In addition, the development of the clock (DN1a)-arousal (Dh44) circuit itself is influenced by the larval nutritional environment. Finally, we demonstrate that larval arousal Dh44 neurons act through glucose metabolic genes to drive onset of daily sleep-wake rhythms. Together, our data suggest that changes to energetic demands in developing organisms trigger the formation of sleep-circadian circuits and behaviors.

Effects of different photoperiods on growth performance, daily rhythm of growth axis-related genes, and hormones in Nile tilapia (Oreochromis niloticus)

The aim of this study was to explore the effects of different photoperiods on the daily rhythm of the growth axis of Nile tilapia (Oreochromis niloticus). Fish were subjected to five photoperiod treatments in this experiment: [light (L) dark (D) ratio: 0 L:24D, 8 L:16D, 12 L:12D, 16 L:8D, 24 L:0D, light intensity 1000 lx]. The growth performance and the daily rhythm of the transcript levels of growth axis-related genes (gh, ghr1, pacap1b, igf-1, igf-1ra) and the contents of growth-related hormones (GH, IGF-1 protein) were analyzed after 4 weeks of culture in the different treatments. To allow for comparisons among treatments, the start of the light period was set to 0 h Zeitberger time (0 h ZT). The results showed that the weight-specific growth rate and daily weight gain of Nile tilapia were highest under 16 L:8D. Under 12 L:12D, the transcript levels of ghr1, igf-1, and igf-1ra and the IGF-1 protein content in the liver showed a clear daily rhythm, and the peak in the daily oscillation (acrophase) was at ZT 7.83 h, ZT 23.53 h, ZT 2.54 h, and ZT 5.91 h, respectively. When the photoperiod changed, ghr1 lost its daily rhythm. Compared with the acrophase under 12 L:12D, the acrophase of igf-1 delayed to ZT 1.71 h under 8 L:16D and ZT 0.06 h under 16 L:8D; that of igf-1ra was delayed to ZT 16.19 h under 16 L:8D and ZT 16.57 h under 24 L:0D; and that of IGF-1 protein was delayed to ZT 8.23 h under 8 L:16D and ZT 8.43 h under 16 L:8D. These results indicated that 16 L:8D was most suitable for the growth of Nile tilapia, because this photoperiod increased the amplitude of IGF-1 including the transcript level and protein and advanced their acrophase. These results shed light on the daily rhythm of growth axis of Nile tilapia, and provided a theoretical basis for optimizing the industrial culture conditions and increasing the yield of this important aquaculture fish species.

Time-restricted feeding during the inactive phase abolishes the daily rhythm in mitochondrial respiration in rat skeletal muscle.

Shift-workers show an increased incidence of type 2 diabetes mellitus (T2DM). A possible mechanism is the disruption of the circadian timing of glucose homeostasis. Skeletal muscle mitochondrial function is modulated by the molecular clock. We used time-restricted feeding (TRF) during the inactive phase to investigate how mistimed feeding affects muscle mitochondrial metabolism. Rats on an ad libitum (AL) diet were compared to those that could eat only during the light (inactive) or dark (active) phase. Mitochondrial respiration, metabolic gene expressions, and metabolite concentrations were determined in the soleus muscle. Rats on AL feeding or dark-fed TRF showed a clear daily rhythm in muscle mitochondrial respiration. This rhythm in mitochondrial oxidative phosphorylation capacity was abolished in light-fed TRF animals and overall 24h respiration was lower. The expression of several genes involved in mitochondrial biogenesis and the fission/fusion machinery was altered in light-fed animals. Metabolomics analysis indicated that light-fed animals had lost rhythmic levels of α-ketoglutarate and citric acid. Contrastingly, lipidomics showed that light-fed animals abundantly gained rhythmicity in levels of triglycerides. Furthermore, while the RER shifted entirely with the food intake in the light-fed animals, many measured metabolic parameters (e.g., activity and mitochondrial respiration) did not strictly align with the shifted timing of food intake, resulting in a mismatch between expected metabolic supply/demand (as dictated by the circadian timing system and light/dark-cycle) and the actual metabolic supply/demand (as dictated by the timing of food intake). These data suggest that shift-work impairs mitochondrial metabolism and causes metabolic inflexibility, which can predispose to T2DM.

Validation of locomotion scoring as a new and inexpensive technique to record circadian locomotor activity in large mammals

BackgroundThe locomotor activity (LA) rhythm, widely studied in rodents, has not been fully investigated in large mammals. This is due to the high cost and the brittleness of the required devices. Alternatively, the locomotion scoring method (SM), consisting of attribution of a score to various levels of activity would be a consistent method to assess the circadian LA rhythm in such species. New methodTo test this, a SM with a score ranging from 0 to 5 has been developed and used in two domestic large mammals, the camel and the goat. One minute interval scoring was performed using visual screening and monitoring of infra-red camera recording videos and carried out by two evaluators. ResultsThe SM provides a clear daily LA rhythm that has been validated using an automate device, the Actiwatch-Mini. The obtained curves and actograms were indeed highly similar to those acquired from the Actiwatch-Mini. Moreover, there were no statistical differences in the period and acrophase. The period was exactly of 24.0h and the acrophases occurred at 12h05 ± 00h03 and 12h14 ± 00h07 for the camel and at 13h13 ± 00h09 and 12h57 ± 00h09 for the goat using SM and Actiwatch-Mini respectively. Comparison with existing methodsCompared to the automatic system, the SM is inexpensive and has the advantage of describing all types of performed movements. ConclusionsThe new developed SM is highly reliable and sufficiently accurate to assess conveniently the LA rhythm and specific behaviors in large mammals. This opens new perspectives to study chronobiology in animal models of desert, tropical and equatorial zones.

An Ultradian Feeding Schedule in Rats Affects Metabolic Gene Expression in Liver, Brown Adipose Tissue and Skeletal Muscle with Only Mild Effects on Circadian Clocks.

Restricted feeding is well known to affect expression profiles of both clock and metabolic genes. However, it is unknown whether these changes in metabolic gene expression result from changes in the molecular clock or in feeding behavior. Here we eliminated the daily rhythm in feeding behavior by providing 6 meals evenly distributed over the light/dark-cycle. Animals on this 6-meals-a-day feeding schedule retained the normal day/night difference in physiological parameters including body temperature and locomotor activity. The daily rhythm in respiratory exchange ratio (RER), however, was significantly phase-shifted through increased utilization of carbohydrates during the light phase and increased lipid oxidation during the dark phase. This 6-meals-a-day feeding schedule did not have a major impact on the clock gene expression rhythms in the master clock, but did have mild effects on peripheral clocks. In contrast, genes involved in glucose and lipid metabolism showed differential expression. In conclusion, eliminating the daily rhythm in feeding behavior in rats does not affect the master clock and only mildly affects peripheral clocks, but disturbs metabolic rhythms in liver, skeletal muscle and brown adipose tissue in a tissue-dependent manner. Thereby, a clear daily rhythm in feeding behavior strongly regulates timing of peripheral metabolism, separately from circadian clocks.

New insights into tambaqui (Colossoma macropomum) feeding behavior and digestive physiology by the self-feeding approach: effects on growth, dial patterns of food digestibility, amylase activity and gastrointestinal transit time

Fish and terrestrial vertebrates usually exhibit daily feeding rhythms that can be associated with daily patterns of digestion dynamics. The classic approach used in feeding studies usually neglects these biological rhythms. Therefore, this study aims to investigate the existence of daily feeding rhythms for tambaqui (Colossoma macropomum), and of daily nutrients and energy digestibility, amylase activity rhythms and gastric evacuation. Four trials were conducted by evaluating: 1) daily feeding rhythms by the self-feeder method; 2) digestibility with two different feeding schedules (midday feeding ML or midnight feeding MD) and assessment of the resulting growth differences; 3) circadian rhythms in amylase activity; 4) the gastric evacuation of the fish fed at the ML or MD. Tambaqui clearly showed nocturnal feeding behavior, with 84.98% of daily food demand occurring at night (p = 0.001). The fish fed at MD grew better than those at ML (p = 0.000), but this result was not followed by improved digestibility (p = 0.500). A clear daily rhythm of amylase activity was observed in fish under both the ML and MD conditions, with an activity peak at night for both groups. Lastly, the tambaqui fed at ML obtained higher intestinal evacuation rates compared to those fed at MD (p = 0.000). Therefore, the results confirm the existence of daily rhythmicity in feeding activity, amylase and evacuation time in tambaqui. Taken together, our results demonstrate that a list of some of tambaquis' digestive physiology and behavioral traits are under circadian control, which should be considered to establish optimized feeding protocols.

Daily rhythm and seasonal pattern of lick use in sika deer (Cervus nippon) in China

Many ungulates are reported to use natural or artificial licks with seasonal patterns around the world. From December 2016 to August 2017, we used infrared camera to record the use of artificial licks in wild sika deer (Cervus nippon) in Zhejiang Qingliangfeng National Nature Reserve, China. We explored the daily rhythm, seasonal pattern and sex difference in lick utilization. In total, 12,043 videos and 22,901 pictures were collected. Our results showed that: (1) the lick visiting frequency was higher at night than that during daytime; (2) the difference in lick visiting frequency between females and males disappeared after taking into account of sex ratio; (3) the lick duration peaked in April during a year. These findings suggested that there were clear daily rhythm and seasonal pattern of lick use in sika deer. Seasonal change in lick use intensity was consistent with our prediction. These variations in lick use might be driven by both the physiological needs of the mineral elements in different life stages and the seasonal changes in climate and food. The reserve management authority should pay more attention to the supplement of licks in spring and summer to fulfill animal’s physiological needs.

Circadian control of prothoracicotropic hormone release in an adult insect and the induction of its rhythmicity by light cues

The insect neuropeptide prothoracicotropic hormone (PTTH) is a critical regulator of larval development. We recently demonstrated that PTTH is also present in adult Rhodnius prolixus and is released by adult brains in vitro with a clear daily rhythm during egg development. Here, we employ a well-established in vitro bioassay, to show that the daily rhythm of PTTH release by brains in vitro is under circadian control since it persists in aperiodic conditions with a free running period of around 24h that is temperature compensated. Prolonged exposure (3weeks) of insects to continuous constant light (LL) completely eliminated PTTH release. Subsequent transfer of such insects from LL to constant darkness (DD) rapidly induced rhythmic PTTH release, indicating that the circadian rhythm of PTTH release is induced by photic cues. Western analysis identified PTTH in the adult hemolymph, suggesting that PTTH acts as a functional neurohormone in the adult insect. Dot blot analysis revealed that PTTH levels in the hemolymph also cycled with a daily rhythm that persisted in DD and was synchronous with the rhythm of PTTH release by brains in vitro. We conclude that the previously documented photosensitive clock in the brain regulates rhythmic PTTH release and thus generates the rhythm seen in the hemolymph. These results emphasize the importance of rhythmic PTTH release in the adult insect and support a role for PTTH in adult physiology and possibly within the adult circadian system.

Effect of age, gender and exercise on salivary dehydroepiandrosterone circadian rhythm profile in human volunteers

There has been a lot of effort by scientists to elucidate the multi functions of the naturally occurring hormone, dehydroepiandrosterone (DHEA). However, to plan research experiments optimally, it is important first to characterize the diurnal rhythm in healthy individuals. The aim of this research was to investigate the daily circadian rhythms of DHEA among the 2 genders, and the effect of age and exercise on salivary DHEA circadian rhythms. Volunteers (20–39 and 40–60years) were recruited for 2 studies investigating the salivary DHEA circadian rhythm. The first study looked at the effect of gender and age on DHEA levels on 2 non-consecutive days, and the second study explored the effect of exercise on DHEA circadian rhythm in males. DHEA levels were estimated by a sensitive and specific ELISA method. The results showed a clear daily circadian rhythm in salivary DHEA in all participants groups, however the profile was flatter in the older female group. There was a significant difference between age and gender groups particularly at 8.00h. In young males DHEA reduced from 541.1±101.3 (mean±sd) at 8.00h to 198.9±90.7pg/mL at 18.00h; p<0.0001, and young females from 401.6±149.5 to 215.4±95.3pg/mL; p<0.001. In older males DHEA reduced from 267.5±32.4 to 132.5±46.7pg/mL; p<0.001, and older females from 147.7±78.1 to 89.5±29.1pg/mL; p=0.05. DHEA levels on 2 non-consecutive days showed some variations but this was not significant. Aerobic exercise has significantly increased DHEA levels at 2 time points of the day (p=0.05) in male subjects. In conclusion, our study showed a clear daily circadian rhythm in salivary DHEA in all participants was observed, but the profile was flatter in the older groups.

Colour change and camouflage in the horned ghost crabOcypode ceratophthalmus

Species that change colour present an ideal opportunity to study the control and tuning of camouflage with regards to the background. However, most research on colour-pattern change and camouflage has been undertaken with species that rapidly alter appearance (in seconds), despite the fact that most species change appearance over longer time periods (e.g. minutes, hours, or days). We investigated whether individuals of the horned ghost crab (Ocypode ceratophthalmus) from Singapore can change colour, when this occurs, and how it influences camouflage. Individuals showed a clear daily rhythm of colour change, becoming lighter during the day and darker at night, and this significantly improved their camouflage to the sand substrate upon which they live. Individuals did not change colour when put into dark conditions, but they did become brighter when placed on a white versus a black substrate. Our findings show that ghost crabs have a circadian rhythm of colour change mediating camouflage, which is fine-tuned by adaptation to the background brightness. These types of colour change can enable individuals to achieve effective camouflage under a range of environmental conditions, substrates, and time periods, and may be widespread in other species. Biological Journal of the Linnean Society, 2013, 109, 257–270.

Glucocorticoid-mediated nycthemeral and photoperiodic regulation of tph2 expression

In the Syrian hamster dorsal and median raphé nuclei, the tryptophan hydroxylase 2 gene (tph2), which codes the rate-limiting enzyme of serotonin synthesis, displays daily variations in its expression in animals entrained to a long but not to a short photoperiod. The present study aimed to assess the role of glucocorticoids in the nycthemeral and photoperiodic regulation of daily tph2 expression. In hamsters held in long photoperiod from birth, after adrenalectomy and glucocorticoid implants the suppression of glucocorticoid rhythms induced an abolition of the daily variations in tph2-mRNA concentrations, a decrease in the amplitude of body temperature rhythms and an increase in testosterone levels. All these effects were reversed after experimental restoration of a clear daily rhythm in the plasma glucocorticoid concentrations. We conclude that the photoperiod-dependent rhythm of glucocorticoids is the main regulator of tph2 daily expression.

Immunohistochemical definition of MT 2 receptors and melatonin in the gastrointestinal tissues of rat

The gastrointestinal tract of vertebrate species contains melatonin, which participates in several physiological functions. Some of these effects are mediated via specific membrane receptors (MT(1) and MT(2)). In the present study, the distribution of the MT(2) receptor protein in the gastrointestinal tract was localized, and changes in MT(2) receptor density were observed in relation to the expected circadian changes in melatonin concentrations. Immunohistochemistry was performed in the rat stomach, duodenum, colon and pancreas. The amount of MT(2) was determined by Western blot. Melatonin concentrations were measured by radioimmunoassay (RIA). In the stomach, duodenum and colon, the most intense immunoreactivity was observed in the Muscularis mucosae and in the Muscularis externa. In the pancreas, the immunolabelling was less intense. There was a clear daily rhythm of melatonin concentrations in the stomach, duodenum and pancreas, with higher levels during the dark period. The density of MT(2) receptors did not exhibit circadian variation. Moreover, circadian changes in melatonin concentrations were not found in the colon. The density of MT(2) was the highest in the colon. Our results provide evidence for the heterogeneous distribution of MT(2) receptors in different parts and layers of the gastrointestinal tract, which could indicate a physiological role of melatonin in the gastrointestinal tract.

Corticosterone in thin-billed prion Pachyptila belcheri chicks: diel rhythm, timing of fledging and nutritional stress

Glucocorticosteroids (GCs) of the hypothalam-pituitary-adrenal axis play a role in association with both stressful events and daily life processes. However, relatively little is known about the role of GCs in relation to daily and seasonal life processes in animals in the wild. In this paper, we present data on basal levels of plasma corticosterone CORT in chicks of a pelagic seabird, the thin-billed prion, Pachyptila belcheri, during two predictable changes in demands, the daily activity pattern and the preparation for fledging. By comparing chicks fed recently with unfed chicks, we test how GC levels are modified according to nutritional condition. In accordance with their nocturnal feeding rhythm, chicks had a clear daily rhythm with increased CORT secretion at night, but CORT levels during the active phase were also highly elevated in unfed chicks compared with fed chicks. Close to fledging, chicks rapidly increased basal CORT levels, and again unfed chicks had higher levels than fed chicks, although the age effect here was stronger than the effect of recent feeding. The present data thus support the hypothesis that GC levels are adjusted to life stages with predictable changes in demands, but food availability and/or internal energy stores also affect the level to which GCs increase.

Sex and housing conditions affect the 24-h acetylcholine release profile in the hippocampus in rats

To examine the sex difference in the 24-h profile of the acetylcholine (ACh) release in the hippocampus, in addition to the effects of housing conditions on this profile, we performed an in vivo microdialysis study in intact male and cycling female rats that had been living in large (diameter=35 cm) or small (diameter=19 cm) cylindrical cages. Each rat was individually housed in a cage for 4 days. On the day of the experiment, the dialysate was collected from the dorsal hippocampus at 20-min intervals and sequential blood samples were simultaneously obtained at 2-h intervals, under the freely moving condition for more than 24 h. ACh in the dialysates was measured by the high performance liquid chromatography system, while the corticosterone concentration in the serum was measured by radiostereoassay. Although the ACh release showed a clear daily rhythm in both sexes of rats, the amount of ACh released in female rats was significantly lower than that in males. Furthermore, the housing in the small cage significantly attenuated the ACh release during the dark phase in male rats, but not in female rats. Conversely, the serum corticosterone concentration showed a clear daily rhythm and the mean concentration of serum corticosterone in female rats was significantly higher than that in male rats. Housing in the small cage did not affect the corticosterone rhythm in either sex. These results reveal a sex difference in the 24-h profile of the ACh release, which suggests vulnerability of the cholinergic system in male rats depending on its housing conditions.

Feeding with powdered diet after weaning affects sex difference in acetylcholine release in the hippocampus in rats

We have reported in the past that female rats fed a powdered diet showed better spatial learning and memory functions than female rats a fed pelleted diet. In the present study, we examined the effects of feeding with powdered diet on acetylcholine release in the hippocampus in both sexes of rats. After weaning (3 weeks of age), rats were fed either standard pelleted diet or powdered diet, and after maturation (9–12 weeks of age), they were used in an in vivo microdialysis study, in which no eserine (a cholinesterase inhibitor) was added to the perfusate. The dialysate was collected from the dorsal hippocampus at 20-min intervals under freely moving conditions for more than 24 h. Acetylcholine in the dialysate was measured by high performance liquid chromatography. As we reported previously, the acetylcholine release showed a clear daily rhythm in both sexes, and males showed significantly greater acetylcholine release in the hippocampus than females in rats fed pelleted diet. Conversely, in rats fed powdered diet, no sex difference in the acetylcholine release was observed, since feeding with powdered diet significantly increased the acetylcholine release only in females. To further examine the number of cholinergic neurons in the medial septum and horizontal limb of the diagonal band of Broca, immunocytochemistry for choline acetyltransferase was performed in both sexes of rats fed either standard pelleted diet or powdered diet. However, neither sex nor feeding conditions affect the number of choline acetyltransferase immunoreactive cells in the areas. These results suggest that powdered diet after weaning enhances spontaneous acetylcholine release in the hippocampus in female rats without changes in the number of cholinergic neurons in the areas. It is possible that this effect of feeding contributes to improve the performance in spatial learning and memory functions in female rats fed powdered diet.

A daily rhythm in mating behavior in a diurnal murid rodent Arvicanthis niloticus

The time of day at which mating occurs is dramatically different in diurnal compared to nocturnal rodents. We used a diurnal murid rodent, Arvicanthis niloticus, to determine if inverted rhythms in responsiveness to hormones contribute to this difference. Male and hormone-primed female grass rats were tested for mating behavior at four different times of day (ZT 5, 11, 17, 23; ZT 0 = lights-on). In females, there was considerable inter-individual variability with respect to patterns of responsiveness to hormones. Overall, the lordosis quotient (LQ) was rhythmic with a single peak just before lights-on (ZT 23); however, while roughly half of the females (7/15) exhibited this clear daily rhythm, the remaining animals (8/15) had relatively high LQs that did not change as a function of time. Males had their shortest ejaculation latencies and their highest number of ejaculations at ZT 23. Rhythms in mount frequency and post-ejaculatory refractory period were bimodal, with peaks around lights-on and -off (ZT 23 and 11). This temporal pattern of mounting behavior closely parallels previously documented patterns of general activity, whereas rhythms in the more reflexive components of sex behavior (LQ and ejaculation) had more restricted peaks that coincided with just the onset of rhythms in general activity. These rhythms in sexual behavior are essentially reversed relative to those previously documented in lab rats.

Effects of moonlight exposure on plasma melatonin rhythms in the seagrass rabbitfish, Siganus canaliculatus.

Influences of light-dark (LD) cycle and moonlight exposure on plasma melatonin rhythms in the seagrass rabbitfish, Siganus canaliculatus, a lunar synchronized spawner, were determined by time-resolved fluoroimmunoassay (TR-FIA). When the fish were exposed to a natural LD (12:12) cycle, plasma melatonin levels exhibited a clear daily rhythm, with higher levels at midnight and lower levels during the day. These rhythms were not evident under either constant light (LL) or constant dark (DD) conditions. Plasma melatonin levels under LL condition were low and high under DD condition. These results indicate that plasma melatonin rhythms are driven by LD cycle in this species. When the fish were exposed to the 4 lunar phases, plasma melatonin levels around the new moon were significantly higher than during the first quarter moon and the full moon. Exposure to experimental new moon and full moon conditions caused significant increases and decreases of plasma melatonin levels, respectively. The synchronous rhythmicity of melatonin levels in the plasma support the hypothesis that the seagrass rabbitfish perceives moonlight intensity and responds with secretion of melatonin into the bloodstream.

Daily rhythms in glucose metabolism: suprachiasmatic nucleus output to peripheral tissue.

The body has developed several control mechanisms to maintain plasma glucose concentrations within strict boundaries. Within those physiological boundaries, a clear daily rhythm in plasma glucose concentrations is present; this rhythm depends on the biological clock, which is located in the hypothalamic suprachiasmatic nucleus (SCN), and is independent of the daily rhythm in food intake. Interestingly, there is also a daily rhythm in glucose uptake, which also depends on the SCN and follows the same pattern as the daily rhythm in plasma glucose concentrations; both rise before the onset of activity. Thus, the SCN prepares the individual for the upcoming activity period in two different ways: by increasing plasma glucose concentrations and by facilitating tissue glucose uptake. In addition to this anticipation of glucose metabolism to expected glucose demands, the SCN also influences, depending on the time of the day, the responses of pancreas and liver to abrupt glucose changes (such as a glucose rise after a meal or hypoglycaemia). This review presents the view that the SCN uses different routes to (i) maintain daily glucose balance and (ii) set the level of the endocrine response to abrupt blood glucose changes.

Pineal melatonin and brain transmitter monoamines in CBA mice during chronic oral nicotine administration

The effects of chronic oral nicotine administration on the pineal melatonin and brain transmitter monoamines were studied in male CBA mice, which possess a clear daily rhythm of melatonin secretion. On the 50th day of nicotine administration, pineal melatonin as well as cerebral dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), norepinephrine (NE), 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG), serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) concentrations were determined at various times. The chronic nicotine treatment did not alter the timing of the pineal melatonin peak, which occurred at 10 h after the light offset. However, in mice drinking nicotine solution, the nocturnal pineal melatonin levels were lower than in control mice drinking tap water. The chronic nicotine treatment increased the striatal DA, DOPAC, HVA and 5-HIAA levels, the hypothalamic NE, MHPG and 5-HIAA and the cortical MHPG. Most prominent effects of nicotine were found at 8 h after the light offset, when the striatal levels of DA and HVA, hypothalamic NE and MHPG as well as cortical MHPG were significantly elevated in the nicotine-treated mice compared with the control mice. No direct correlation between nicotine’s effects on brain transmitter monoamines and on pineal melatonin levels was apparent. The results suggest that chronic nicotine treatment slightly suppresses the melatonin production but does not alter the daily rhythm of pineal melatonin in mice maintained on a light–dark cycle. However, the results indicate that nicotinic receptors might be involved in the regulation of pineal function.