European Age Research Articles

Composite dyadic models for spatio-temporal data.

Mechanistic statistical models are commonly used to study the flow of biological processes. For example, in landscape genetics, the aim is to infer spatial mechanisms that govern gene flow in populations. Existing statistical approaches in landscape genetics do not account for temporal dependence in the data and may be computationally prohibitive. We infer mechanisms with a Bayesian hierarchical dyadic model that scales well with large data sets and that accounts for spatial and temporal dependence. We construct a fully connected network comprising spatio-temporal data for the dyadic model and use normalized composite likelihoods to account for the dependence structure in space and time. We develop a dyadic model to account for physical mechanisms commonly found in physical-statistical models and apply our methods to ancient human DNA data to infer the mechanisms that affected human movement in Bronze Age Europe.

Establishment of clary sage (Salvia sclarea L.) regeneration system and development of glyphosate-tolerant clary sage plants through Agrobacterium-mediated genetic transformation

Clary sage (Salvia sclarea L.) is well known as a highly aromatic herbaceous plant and is commonly used in aromatic products and medicine. The biggest problem in the cultivation of clary sage is weeds, which not only entails a great cost of labor for weeding, but also leads to a great fluctuation in the yield and quality of its products. Therefore, there is a need to breed herbicide-resistant clary sage using genetic engineering. However, the lack of suitable regeneration and genetic transformation systems for clary sage has limited the process of molecular breeding. In this study, we firstly reported the regeneration system of clary sage, where explants from hypocotyls of sage induced adventitious shoots at a rate of 22.22% when cultured on MS medium supplemented with 2.5mg·L-1 6-BA, 0.15mg·L-1 IAA, and 0.25mg·L-1 VC. Rooting of adventitious shoots was achieved at a rate of 94.44% on 1/2 MS medium containing 0.125mg·L-1 NAA, 0.5mg·L-1 IAA, and 0.1mg·L-1 IBA. Secondly, based on the regeneration system, an efficient and stable Agrobacterium-mediated genetic transformation system was established in this paper, and the rate of genetic transformation was determined to be in the range of 3%-5% by transforming the hypocotyls of clary sage with pCAMBIA1301 vector and pCAMBIA1301-GR79-GAT vector. Finally, glyphosate tolerance was obtained from GR79 and GAT co-expressed clary sage plants in this study. This study not only lays the foundation of genetic transformation for the functional gene research and in-depth investigation of various biological problems of clary sage, but also provides a feasible genetic engineering solution for its industry to solve the weed problem, as well as a promotional role for precise molecular breeding of clary sage.

Deterrent Effects of Clary Sage Oil and Two Major Constituents against Drosophila suzukii (Diptera: Drosophilidae).

Drosophila suzukii (Diptera: Drosophilidae), spotted-wing drosophila, poses a significant threat to soft-skinned fruit crops in the Americas, Europe, Africa, and Oceania, as well as in Asia. The application of chemical insecticides is the primary control strategy for D. suzukii; however, resistance has developed with the indiscriminate use of chemical insecticides. Essential oils, considered potential alternatives to pesticidal strategies, exhibit potent toxic and sublethal behavioral effects against numerous pests, including D. suzukii. Clary sage oil repels a variety of agricultural and household pests; however, whether it has a repellent effect against D. suzukii remains unknown. Here, we found that clary sage oil exhibited dose-dependent repellency against D. suzukii adults in a T-maze assay, a two-choice assay and a two-choice attraction assay. Also, clary sage oil showed a significant repellent effect against D. suzukii larvae. Next, we explored the chemical constituents of clary sage oil by GC-MS and identified two major constituents, linalyl acetate (40.03%) and linalool (23.02%). Furthermore, the behavioral assays of linalyl acetate and linalool showed that both compounds conferred comparable repellency against D. suzukii adults and larvae. Finally, we found clary sage oil, linalyl acetate, and linalool elicited EAG responses in D. suzukii, especially clary sage oil, suggesting the repellency was mediated by the olfactory system. These findings indicate that D. suzukii shows olfactory-based behavioral avoidance of clary sage oil, linalyl acetate, and linalool. Clary sage oil and its major constituents may be possible alternatives in the management of D. suzukii.

Molecular docking analysis of Salvia sclarea flower extracts evaluated for protein target affinity based on different extraction methods

AbstractThis study evaluates the antioxidant activity and total phenolic, flavonoid, flavonol, and tannin contents of extracts obtained from the flower parts of Salvia sclarea using different extraction methods, such as maceration and rotavapor (RE). The results indicate that the maceration method yields extracts with higher antioxidant activity compared to the rotavapor method (DPPH IC50: 260.70 ± 65.41 mg/mL vs. 345.48 ± 27.91 mg/mL). Additionally, the maceration method produces higher values in terms of total phenolic (26.4 ± 7.78 mg GAE/g extract), flavonoid (10.44 ± 0.21 mg QE/g extract), flavonol (9.20 ± 0.84 mg QE/g extract), and tannin contents (8.36 ± 0.39 mg GAE/g extract), whereas the RE method shows lower values (phenolics: 19.8 ± 3.31 mg GAE/g extract, flavonoids: 10.35 ± 0.35 mg QE/g extract, flavanol's: 5.45 ± 0.01 mg QE/g extract, and tannins: 7.72 ± 0.10 mg GAE/g extract). The gas chromatography–mass spectrometry (GC–MS) analysis of the extracts identified various bioactive compounds. Molecular docking studies revealed that Terpinen‐4‐ol obtained through maceration exhibits a strong binding affinity to a specific protein target, indicating potential biological activity. The predicted pharmacokinetic (ADME (absorption, distribution, metabolism, and excretion)) parameters for Terpinen‐4‐ol suggest favorable absorption and central nervous system (CNS) penetration, while Squalene obtained through maceration shows different pharmacokinetic properties. S. sclarea highlights significant antioxidant potential and provides insight into the bioactive compounds obtained through different extraction methods. To further investigate the potential biological interactions arising from these differences, molecular docking studies were conducted.

Multi-environment evaluation of Clary sage (Salvia sclarea L.) selections for yield and essential oil traits under Western Himalayan conditions

Clary sage is an important aromatic herb that has a high potential for commercial cultivation under the diversified climates of the Western Himalayas. The present study was carried out to understand the performance of genotypes and their interaction with the varied environments prevalent in the Western Himalayas and to identify stable and best-performing genotypes. In the present experiment, ten superior selections were evaluated in four different environments as multi-location trials in a randomized block design with three replications during 2021 and 2022. The pooled analysis of variance revealed significant variations for genotypes, environments, and genotype × environment interaction for all the studied traits. The highest mean performance suggests that Env-1 has the most suitable conditions for clary sage cultivation. The genotypes CSIR-IHBT-SS-07 and CSIR-IHBT-SS-09 were found to be the best performers for fresh inflorescence weight (418.73 g/plant) and essential oil content (428.91 mg/Kg), respectively. The results of Eberhart and Russell’s regression-based model confirm that the genotype CSIR-IHBT-SS-07 is a stable and superior genotype for the economically important traits. Further, the gas-chromatography mass spectroscopic characterization of essential oil disclosed that CSIR-IHBT-SS-07 was unique in terms of the highest sclareol content (23.39–45.17 %) across all the test environments. Genotype + Genotype × Environment biplot analysis confirmed that all the test environments form a single mega-environment. The study is a pioneer in unraveling the stability response of clary sage in Western Himalaya and provides a strong base for the identification of superior genotypes and selection strategy for future genetic improvement programs of clary sage.

PSII-12 The effect of the diffusion of geranium and clary sage essential oils on relaxation responses in horses

Abstract Aromatherapy has been used in holistic medicine to improve relaxation in humans; however, there are limited studies on its use in horses. This study investigated the potential of aromatherapy on relaxation responses in horses using two common essential oils geranium (Pelargonium asperum) and clary sage (Salvia sclarea). We hypothesized that aromatherapy using essential oils would improve relaxation responses in horses. Geranium aromatherapy (GA) and clary-sage aromatherapy (CA) were tested against a control (CO; deionized water only) in a replicated 3 x 3 Latin square with three treatments, three horses (584 ± 9 kg of body weight and 11 ± 1 yr old), and three experimental periods. The experiment was replicated using the same horses, and the two experimental replicates were considered the square blocking factor. Aromatherapy session involved a 5-min acclimation period followed by 15 min of aromatherapy using a diffusor. Heart rate and respiration rate at min 0, 7 and 15, and average counts for body position and facial expressions from min 0 to 7 and from 7 to 15 (early and late session, respectively) were used as measure of relaxation. Aromatherapy improved eye expression over time (P = 0.01), with GA and CA horses showing significantly increased durations of relaxed eyes from early to late session (2.8 min vs. 5.3 min for GA and 3.7 min vs. 4.8 min for CA), while horses on CO showed reduced duration from early to late session (2.7 min vs. 1.2 min), indicating increased level of alertness and tension. Aromatherapy tended (P = 0.07) to improve ear expression. Horses receiving CA tended to show longer durations of relaxed ears compared with CO (4.33 min vs. 1.25 min), while GA (3.25 min) did not differ from CA and CO. Aromatherapy did not affect (P > 0.05) measures of body posture and heart and respiratory rates. Although not all relaxation responses were improved by aromatherapy, the results indicate that aromatherapy has potential for improving relaxation in horses as indicated by facial expressions.

Insecticidal and Repellent Activity of Essential Oils from Copaifera reticulata, Citrus paradisi, Lavandula hybrida and Salvia sclarea Against Immature and Adult Stages of Ctenocephalides felis felis.

The flea Ctenocephalides felis (Siphonaptera: Pulicidae), parasitizes dogs and cats globally, acting as a vector for various pathogens affecting both animals and humans. Growing interest in environmentally friendly, plant-based products prompted this study. The aim of the study was to determine the chemical composition of essential oils (EOs) from Copaifera reticulata, Citrus paradisi, Lavandula hybrida and Salvia sclarea, assessing their insecticidal and repellent properties, determining lethal concentrations (LC50 and LC90), and evaluating residual efficacy in vitro against Ctenocephalides felis felis. Gas Chromatography with Flame Ionization Detector analyzed EO composition. In vitro tests involved preparing EO solutions at various concentrations. Ten specimens from each life stage (egg, larva, pupa, adult) were used for insecticidal activity assessment. Adulticidal activity was assessed using 10cm2 filter paper strip, each treated with 0.200mL of the test solution. Immature stages activities were evaluated using 23.76cm2 discs of the same filter paper, each treated with 0.470mL of the test solution. Mortality percentage was calculated using (number of dead insects × 100) / number of incubated insects. Probit analysis calculated LC50 values with a 95% confidence interval. Major EO constituents were β-caryophyllene (EOCR), linalool (EOLH), linalyl acetate (EOSS), and limonene (EOCP). LC50 values were obtained for all stages except for the essential oil of C. paradisi. All oils showed repellent activity at 800μg/cm2. OECR exhibited greater residual efficacy. Each EO demonstrated superior insecticidal activity against specific C. felis felis stages.

SYNERGISTIC EFFECT OF HERBAL TEA SAMPLES WITH CIPROFLOXACIN AGAINST MRSA AND THEIR ANTIOXIDANT ACTIVITIES

OBJECTIVE: Sambucus nigra L. (elderberry), Salvia sclarea L. (clary sage), Rosmarinus officinalis L. (rosemary), and Coriandrum sativum L. (coriander) are all consumed as flavoring agents for food and are well-known in traditional medicine for the treating various diseases worldwide. These herbs are also commonly used for microbial infections, especially to relieve cough, fever, and cold symptoms. This study aims to screen and compare the content of total phenols and antioxidant activity potential of three different extracts from each commercial sample of S. nigra, S. sclarea, R. officinalis and C. sativum from Türkiye. We also investigated the (Minimum Inhibitory Concentration (MIC) values against Staphylococcus aureus ATCC 29213 (methicillin-susceptible, MSSA) and Staphylococcus aureus ATCC 43300 (methicillin-resistant, MRSA) and the synergistic activity with an antibiotic, ciprofloxacin, by checkerboard assay. MATERIAL AND METHODS: The fruits of S. nigra and C. sativum, the aerial parts of S. sclarea, and the leaves of R. officinalis were purchased from a trading company that marketed them as herbal tea in Türkiye. This study investigated the total phenolic content and antioxidant potential of extracts from commercial samples. We also determined the synergistic effect of herbal tea samples with ciprofloxacin against methicillin-resistant Staphylococcus aureus (MRSA). RESULTS: R. officinalis (rosemary) and Salvia sclarea (clary sage) extracts showed high phenolic content and antioxidant activity, whereas it was determined that C.sativum (coriander) and S. nigra (elderberry) extracts exhibited lower antioxidant activity and low phenolic compounds. Moreover, ciprofloxacin in combination with the ethanolic extract of S. sclarea showed additive antibacterial activity against S. aureus ATCC 43300. CONCLUSIONS: We conclude that herbal tea prepared from commercial R. officinalis (rosemary) and S.sclarea (clary sage) samples can support the antioxidant activity with high phenolic content and that ciprofloxacin combined with the ethanolic extract of clary sage showed additive antibacterial activity against methicillin-resistant S. aureus ATCC 43300. Further studies are needed to understand the mechanism of additive action in detail.

Exploring the Efficacy of Four Essential Oils as Potential Insecticides against Thrips flavus

Plant essential oils are important alternatives in green integrated pest management. This study examined the chemical composition, bioactivity, and control efficacy of four Lamiaceae essential oils (EOs) against Thrips flavus Schrank in laboratory conditions with the goal of exploiting plant-derived insecticides to control Thrips flavus. The four EOs tested were marjoram oil (Origanum majorana L.), clary sage oil (Salvia sclarea L.), perilla leaf oil (Perilla frutescens (L.) Britt.), and spearmint oil (Mentha spicata L.). All these EOs exhibited a certain degree of insecticidal activity against Thrips flavus. The median lethal concentration (LC50) was determined after treatment by the leaf-dipping method in laboratory bioassays, and its values were 0.41 mg/mL for marjoram oil, 0.42 mg/mL for clary sage oil, 0.43 mg/mL for perilla leaf oil, and 0.54 mg/mL for spearmint oil. In the pot experiment, the number of dead insects was recorded at 1, 3, and 7 days post-application, and the control efficacy of EOs against Thrips flavus was calculated. The concentration of 900.00 g a.i.·hm−2 of spearmint oil was 100% lethal against Thrips flavus after treating potted plants for seven days. The Y-tube olfactometer method was used to test for the attraction or repellent response of EOs against Thrips flavus. The spearmint oil significantly attracted female adults in the olfactory test. Furthermore, gas chromatography–mass spectrometry (GC–MS) was used to examine the chemical composition of the EOs. Linalool (24.52%), isopropyl myristate (28.74%), (+)-limonene (32.44%), and (+)-carvone (70.3%) were their primary ingredients. The findings suggest that all four EOs are highly effective against Thrips flavus and may be a possible alternative in the management of Thrips flavus, especially when considering reducing the use of synthetic pesticides.

Linalyl acetate exerts analgesic effects by inhibiting nociceptive TRPA1 in mice.

Clary sage essential oil (CSEO) is utilized in perfumery, aromatherapy, and skincare. Linalyl acetate (LA), a primary component of CSEO, possesses sedative, anxiolytic, and analgesic properties. However, the mechanism of its analgesic action is not clearly understood. Transient receptor potential ankyrin 1 (TRPA1) channel, a non-selective cation channel, is mainly expressed in sensory neurons and serves as a sensor of various irritants. In this study, we investigated the effects of LA on TRPA1 channel using heterologous expression system and isolated sensory neurons. To detect channel activity, we employed Ca2+ imaging and the whole-cell patch-clamp technique. The analgesic action of LA was measured in a pain-related behavioral mouse model. In cells that heterologously expressed TRPA1, LA diminished [Ca2+]i and current responses to allylisothiocyanate (AITC) and carvacrol: exogenous TRPA1 agonists, and the inhibitory effects were more pronounced for the former than for the latter. Moreover, LA suppressed [Ca2+] i and current responses to PGJ2: an endogenous TRPA1 agonist. Similar inhibitory actions were observed in native TRPA1 channels expressed in mouse sensory neurons. Furthermore, LA diminished PGJ2-induced nociceptive behaviors in mice. These findings suggest that analgesic effects of LA exert through inhibition of nociceptive TRPA1, making it a potential candidate for novel analgesic development.

Effect of Salt (NaCl) Stress on Germination in Clary Sage (Salvia sclarea) Subjected to Gibberellic Acid Pre-treatment

Effect of Salt (NaCl) Stress on Germination in Clary Sage (Salvia sclarea) Subjected to Gibberellic Acid Pre-treatment

‘Princely seats’ and Thessalian hillforts: pre-urban Greece and the diffusion of urbanism in Early Iron Age Europe

The origins of Iron Age urbanism in temperate Europe were long assumed to lie in Archaic Greece. Recent studies, however, argue for an independent development of Hallstatt mega-sites. This article focuses on developments in Western Thessaly in mainland Greece. The author characterises the Archaic settlement system of the region as one of lowland villages and fortified hilltop sites, the latter identified not as settlements but refuges. It is argued that cities were rare in Greece prior to the Hellenistic period so its settlements could not have served as the model for urban temperate Europe. Consequently, the social and political development of Greece and temperate Europe followed different trajectories.

Effect of Salvia sclarea L. extract on growth performance, antioxidant capacity, and immune function in lambs.

This study aimed to explore the effects of Salvia sclarea extract on the growth performance, apparent nutrient digestibility, antioxidant capacity, and immune function of the lambs. Sixty female lambs (Chinese Merino sheep) aged 2 months and weighing 20 ± 2 kg were selected and randomly divided into five groups of 12 lambs each. The control group (CK) received only basal feed, whereas the experimental group was supplemented with different concentrations of salvia sclarea extract in the basal feed at 0.04, 0.08, 0.12, and 0.16 mL/kg (CL1, CL2, CL3, and CL4, respectively). The feeding period was 85 days, including 15 days of pre-feeding and 70 days of regular feeding. Body weight and feed intake were recorded during the test period, and blood was collected at the end of the test to determine immune and antioxidant indices. The results showed that the average daily weight gain and feed intake of the lambs were significantly higher in the CL3 group than in the CK group (p < 0.05). In addition, the apparent nutrient digestibility of crude protein and neutral detergent fiber increased significantly (p < 0.05). The dry matter, acid detergent fiber, and ether extract were not significantly different (p > 0.05). Serum levels of superoxide dismutase, catalase, and glutathione peroxidase and antioxidant capacity were significantly higher in the CL2, CL3, and CL4 groups than in the CK group, whereas malondialdehyde levels were significantly lower (p < 0.05). The serum levels of immune globulin immune globulin A, immune globulin G, immune globulin M, interferon-γ, and interleukin-10 were significantly higher and the levels of tumor necrosis factor-α and interleukin-1β were significantly lower in the CL2, CL3, and CL4 groups (p < 0.05). In conclusion, the addition of the S. sclarea extract to the diet promoted growth performance and nutrient digestion in lambs. Immune response was improved by increasing Ig and cytokine concentrations. It enhances antioxidant status by increasing antioxidant enzyme activity in lambs.

Synergistic antibacterial effects of Trachyspermum ammi L. essential oil and sodium nitrite in combination on artificially inoculated food models

The addition of carminative essential oils could be an approach for food preservation and would minimize or substitute chemical preservatives. In the present study, essential oils (n=11) namely, Anethum sowa, Cinnamomum zeylanicum, Citrus bergamia, Cymbopogon flexuosus, Cymbopogon martini, Cymbopogon winterianus, Elettaria cardamomum, Mentha arvensis, Ocimum basilicum, Salvia sclarea and Trachyspermum ammi, were screened against Aeromonas hydrophila and Listeria monocytogenes. The largest diameters of zone of inhibition, 19.9 ± 0.33 mm and 21.7 ± 0.58 mm, were exhibited by T. ammi essential oil against Aeromonas hydrophila and Listeria monocytogenes, respectively. Growth inhibition studies for T. ammi essential oil, sodium nitrite and their combinations were also carried out on cucumber, apple, gram flour soup and mutton broth models. The combination of T. ammi essential oil and sodium nitrite depicted synergism and was also effective in reducing the bacterial counts in artificially inoculated food systems.

The study of the diuretic activity and pharmacotechnological properties of a dry extract of Salvia sclarea L. growing in Tajikistan

Aim. To study the pharmacological activity of a dry extract of clary sage (DECS) growing in Tajikistan and its pharmacotechnological properties as basic stages in the development of the composition and technology of hard gelatin capsules with its content. Materials and methods. For the studies, we used DECS standardized by the content of the total amount of flavonoids and hydroxycinnamic acids. The quantitative content of the total amount of flavonoids was not less than 13.0 % calculated with reference to apigenin, and the quantitative content of the total amount of hydroxycinnamic acids was not less than 1.2 % calculated with reference to rosmarinic acid. The pharmacotechnological properties of DECS were studied according to methods of the State Pharmacopoeia of Ukraine (SPhU). Results and discussion. During screening, all doses of DECS studied showed a moderate diuretic activity. Thus, when using DECS in the dose of 100 mg/kg, the relative volume of the urine excreted by rats for 5 hours increased by 1.9 times, in the dose of 200 mg/kg – by 2.0 times, in the dose of 300 mg/kg – by 1.8 times compared to the same indicator in the negative control group (p˂0.05). When studying the complex of pharmacotechnological properties, it was found that DECS was a finely dispersed amorphous powder with particles of isodiametric form. DECS had an average bulk weight of 0.515 ± 0.002 g/mL, and the fluidity assessment showed that DECS had a very poor flowability, which value in the vibration mode of the device was 2.8 ± 0.1 g/s. According to the study of the fractional composition, DECS had a clearly expressed fine fraction with a particle size of less than 0.25-0.09 mm 84.68 %. Conclusions. Taking into account the results of the study of the diuretic activity and scientific literature data on the spectrum of the pharmacological activity of Salvia sclarea L, it is rational to consider DECS as a potential combined agent for enhancing the diuretic effect or in diseases of the urinary system accompanied by edema and inflammatory processes. The study of the pharmacotechnological indicators of DECS allows predicting the need to use certain groups of excipients to develop the composition and technology of capsules with its content, namely antifriction substances to improve fluidity and disintegrants to improve capsule disintegration, as well as substances that contribute to the compaction of bulk mass and moisture-regulating agents.

Photosystem II Tolerance to Excess Zinc Exposure and High Light Stress in Salvia sclarea L.

High light (HL) intensity has a substantial impact on light energy flow and partitioning within photosynthetic apparatus. To realize the impact of HL intensity on zinc (Zn) tolerance mechanisms in clary sage (Salvia sclarea L., Lamiaceae) plants, we examined the effect of the altered chlorophyll and nutrient uptake under excess Zn supply on the response mechanism of photosystem II (PSII) photochemistry. Eight-week-old clary sage plants were treated with 5 μM Zn (control) or 900 μM Zn in Hoagland nutrient solution. Leaf elemental analysis for Zn, Mn, Mg, and Fe was performed by inductively coupled plasma mass spectrometry (ICP-MS), whereas PSII functioning under HL was evaluated by chlorophyll fluorescence imaging analysis. Exposure of S. sclarea plants to 900 μM Zn increased leaf Zn accumulation and decreased leaf Mg and chlorophyll. The decreased non-photochemical quenching (NPQ) provided evidence of the photoprotection offered by the smaller light-harvesting antennae due to the reduced chlorophyll. The increased Mn after Zn exposure corresponded with higher efficiency of the oxygen-evolving complex (OEC) that was significantly correlated with the maximum efficiency of photosystem II (PSII) photochemistry (Fv/Fm). An increased electron transport rate (ETR) coincided with increased leaf Fe, which is known to play a vital role in the enzymes engaged in ETR. The decreased (32%) NPQ after an 8-day exposure to Zn caused an increased (10%) quantum yield of non-regulated energy loss in PSII (ΦNO), indicative of an increased singlet oxygen (1O2) production. It is suggested that the decreased NPQ induced acclimation responses of clary sage plants to HL and excess Zn by increasing 1O2 production. The reduced (18%) excess excitation energy (EXC) at PSII and the increased (24%) quantum yield of PSII photochemistry (ΦPSII) and ETR indicated improved photosynthetic efficiency under excess Zn and HL intensity. Therefore, the exposure of medicinal plants to excess Zn not only boosts their photosynthetic efficiency, enhancing crop yields, but can also improve Fe and Zn content, ameliorating the human health deficiency of these two essential micronutrients.

Today’s Enlightenment

This paper aims to present Derrida’s reflections on Europe, which criticizes Europe’s actuality while recognizing its promise to-come. Europe in modernity has been conceptualized as sovereign heading, the only one capable of governing the world. Derrida hopes for a Europe other than a super-state, closed within its borders, and an economic alliance. He works on today’s Enlightenment, that is, on a new shape of Europe as a shoreline fit to welcome the other, following in the footsteps of Kant, who redefined the right of hospitality and drafted a cosmopolitan order, but going beyond his sketch. In the global age Europe must exemplarily assume The law of hospitality as its key principle, constantly seeking to translate it into political and legal acts.

Sheep's Second Cheese Whey Edible Coatings with Oregano and Clary Sage Essential Oils Used as Sustainable Packaging Material in Cheese.

Sheep's second cheese whey (SCW), the by-product resulting from whey cheese production, was used as a component of cheese coatings containing oregano (Origanum compactum) and clary sage (Salvia sclarea) essential oils (EOs). SCW powder was obtained by the ultrafiltration/diafiltration of SCW followed by reverse osmosis and freeze drying. The coatings were produced with a mixture of SCW and whey protein isolate (WPI) using glycerol as plasticizer. Model cheeses were produced with cow´s milk and those containing SCW:WPI coatings; those with and without EOs were compared to controls without coating and with a commercial coating containing natamycin. At the end of ripening (28 days), the cheeses containing EOs presented higher water activity (ca. 0.930) and moisture content, as well as lower titratable acidity. Concerning color parameters, significant differences were also observed between products and as a result of ripening time. However, the use of SCW:WPI coatings did not significantly influence the color parameters at the end of ripening. Regarding texture parameters, the cheeses containing SCW:WPI coatings presented significantly lower values for hardness, chewiness, and gumminess. Significant differences were also observed for all microbial groups evaluated either between products and as a result of ripening time. In all cases, lactobacilli and lactococci counts surpassed log 7-8 CFU/g, while the counts of yeasts and molds increased steadily from ca. log 3 to log 6 CFU/g. The lowest counts of yeasts and molds were observed in the samples containing natamycin, but nonsignificant differences between products were observed. In conclusion, SCW:WPI cheese coatings can successfully substitute commercial coatings with the advantage of being edible packaging materials manufactured with by-products.

Green synthesis of AgNPs from leaves extract of Saliva Sclarea, their characterization, antibacterial activity, and catalytic reduction ability

Abstract Several technologies are employed for the synthesis of silver nanoparticles, each synthesis technique has advantages and disadvantages, and the best technique relies on the application at hand, the required qualities of the nanoparticles, and the size of the product. But in this article green synthesis were followed. In this research, AgNPs were synthesized using Salvia Sclarea leaf extract in green synthetic routes. The synthesized nanoparticles were examined using UV–vis spectroscopy, powder XRD, SEM, and FT-IR. Here three different type of silver nanoparticles were biosynthesized, AgNPs-1, AgNPs-2, and AgNPs-3 (where composition of AgNO3 and extract were 6:1, 10:1 and 14:1 respectively). The catalytic ability of AgNPs 1–3 was determine in the reduction of nitro-compounds into corresponding amines, where AgNPs-2 was found efficient reductive catalyst. Moreover, antibacterial activities were checked against both gram-positive (Bacillus Suntilis) and gram-negative bacteria (Klebsiella pneumoniae). Upon increasing Ag contents antibacterial activities were found in increasing mode. Which open new era of knowledge for further consideration.

Understanding money; Or, why social and financial accounting should not be conflated

AbstractThis article defines social and financial money as distinct institutions that account for different realms of value. I present a fundamental dichotomy among economists' where orthodox theory defines money as a medium of exchange whereas heterodox chartalist economists characterize it as a unit of account. I argue that (pre)historical data provides clear evidence in support of the heterodox position. The unit of account function of money is exemplified by how wampum accounted for social debts and was expanded to also serve financial functions by European colonial governments. The heterodox position is further evidenced with the metal coins that denominated Rome's financial money that transitioned to serve primarily social purposes in early Anglo‐Saxon Britain. Focusing on the accounting function of social and financial monies transcends the Polanyian special‐versus‐general‐purpose framework that often still structures archaeological practice. With this framework of money defined by what gives it value, I then evaluate recent claims that financial money was integral to the political economies of Bronze Age Europe. I conclude that the adoption of the orthodox assumption that money is primarily a medium of exchange inhibits understanding of what money is and how the political economies of ancient societies were organized.