Clary Sage Oil Research Articles

Deterrent Effects of Clary Sage Oil and Two Major Constituents against Drosophila suzukii (Diptera: Drosophilidae).

Drosophila suzukii (Diptera: Drosophilidae), spotted-wing drosophila, poses a significant threat to soft-skinned fruit crops in the Americas, Europe, Africa, and Oceania, as well as in Asia. The application of chemical insecticides is the primary control strategy for D. suzukii; however, resistance has developed with the indiscriminate use of chemical insecticides. Essential oils, considered potential alternatives to pesticidal strategies, exhibit potent toxic and sublethal behavioral effects against numerous pests, including D. suzukii. Clary sage oil repels a variety of agricultural and household pests; however, whether it has a repellent effect against D. suzukii remains unknown. Here, we found that clary sage oil exhibited dose-dependent repellency against D. suzukii adults in a T-maze assay, a two-choice assay and a two-choice attraction assay. Also, clary sage oil showed a significant repellent effect against D. suzukii larvae. Next, we explored the chemical constituents of clary sage oil by GC-MS and identified two major constituents, linalyl acetate (40.03%) and linalool (23.02%). Furthermore, the behavioral assays of linalyl acetate and linalool showed that both compounds conferred comparable repellency against D. suzukii adults and larvae. Finally, we found clary sage oil, linalyl acetate, and linalool elicited EAG responses in D. suzukii, especially clary sage oil, suggesting the repellency was mediated by the olfactory system. These findings indicate that D. suzukii shows olfactory-based behavioral avoidance of clary sage oil, linalyl acetate, and linalool. Clary sage oil and its major constituents may be possible alternatives in the management of D. suzukii.

Exploring the Efficacy of Four Essential Oils as Potential Insecticides against Thrips flavus

Plant essential oils are important alternatives in green integrated pest management. This study examined the chemical composition, bioactivity, and control efficacy of four Lamiaceae essential oils (EOs) against Thrips flavus Schrank in laboratory conditions with the goal of exploiting plant-derived insecticides to control Thrips flavus. The four EOs tested were marjoram oil (Origanum majorana L.), clary sage oil (Salvia sclarea L.), perilla leaf oil (Perilla frutescens (L.) Britt.), and spearmint oil (Mentha spicata L.). All these EOs exhibited a certain degree of insecticidal activity against Thrips flavus. The median lethal concentration (LC50) was determined after treatment by the leaf-dipping method in laboratory bioassays, and its values were 0.41 mg/mL for marjoram oil, 0.42 mg/mL for clary sage oil, 0.43 mg/mL for perilla leaf oil, and 0.54 mg/mL for spearmint oil. In the pot experiment, the number of dead insects was recorded at 1, 3, and 7 days post-application, and the control efficacy of EOs against Thrips flavus was calculated. The concentration of 900.00 g a.i.·hm−2 of spearmint oil was 100% lethal against Thrips flavus after treating potted plants for seven days. The Y-tube olfactometer method was used to test for the attraction or repellent response of EOs against Thrips flavus. The spearmint oil significantly attracted female adults in the olfactory test. Furthermore, gas chromatography–mass spectrometry (GC–MS) was used to examine the chemical composition of the EOs. Linalool (24.52%), isopropyl myristate (28.74%), (+)-limonene (32.44%), and (+)-carvone (70.3%) were their primary ingredients. The findings suggest that all four EOs are highly effective against Thrips flavus and may be a possible alternative in the management of Thrips flavus, especially when considering reducing the use of synthetic pesticides.

Characterization of biodegradable films based on guar gum and calcium caseinate incorporated with clary sage oil: Rheological, physicochemical, antioxidant, and antimicrobial properties

Clary sage essential oil (CSEO) has been reported for its wide food and pharmaceutical applications. This work aimed to produce biodegradable active guar gum-calcium caseinate (GG-CC) based film incorporated with CSEO and to evaluate its physico-chemical, antioxidant, and antibacterial properties. Solvent casting technique was used to prepare the composite films containing CSEO at 0.1, 0.15, and 0.2% (v/v). The chemical, rheological, morphology, mechanical, thermal, optical, crystalline, biodegradable, water barrier, antioxidant and antimicrobial potential of the samples were assessed by various analytical methods. Rheological findings of filmogenic solution revealed that the viscosity of filmogenic solutions (GCEO2–GCEO4) increased with an increase in the concentration of the CSEO. CSEO addition in GG-CC film exhibited a homogenous structure with low crystallinity, transparency, water vapor permeability, moisture content, and tensile strength values, whereas a rise in elongation at break, thickness, and thermal stability was observed. Fourier transforms infrared (FTIR) results suggested the interaction between CSEO and GG-CC-CSEO films also exhibited satisfactory antioxidant activity against DPPH and ABTS free radicals and higher antimicrobial effect against the Gram-negative bacteria (Pseudomonas aeruginosa). This work revealed that CSEO has the potential to be used as an active ingredient in the development of films for food packaging applications.

Transdermal Absorption of Sclareol, an Active Ingredient in Clary Sage Oil: A Complementary and Alternative Medicine for Menopausal Symptoms

Clary sage oil is commonly used in complementary and alternative medicine to treat menopausal symptoms. However, whether sclareol, which is considered to have estrogen-like effects, can be absorbed transdermally remains unknown. Therefore, in this study, we applied sclareol dissolved in jojoba oil to the dorsal skin of male Hos-HR-1 mice 30 min before blood sampling. We examined whether sclareol can be detected in the plasma because of transdermal absorption using gas chromatography-mass spectrometry analysis. The concentration of sclareol in plasma and liver samples calculated based on the measured sample concentration and the recovery rate was 0.36 ± 0.08 and 1.69 ± 0.32 ppm, respectively. Furthermore, there was a significant positive correlation between the plasma sclareol concentration and hepatic homogenate sclareol concentration. Our findings indicate that sclareol is absorbed transdermally and accumulates in the liver. Moreover, the lack of change in plasma blood urea nitrogen, aspartate aminotransferase, and alanine aminotransferase levels among the three groups indicates that there was no hepatic or renal damage due to transdermal absorption of sclareol in Hos-HR-1 mice. However, further validation in humans is required because the thickness of the dermis and the number of pores significantly differ between mice and humans.

Water solubility of clary sage essential oil

The article provides data on the degree of solubility of essential oils in water. Essential oils are considered as compounds that are completely insoluble or slightly soluble, or refer to substances with a certain, limited, solubility. The distillation of the essential oil from the raw material is preceded by its dissolution in water and hydrodiffusion in the solution beyond the limits of the essential oil containers. The solubility of essential oils in water, apparently, plays an important role in living plants during their intracellular movement and removal outside the cells. To clarify the concepts, samples of primary and secondary oils obtained in production have been examined. The solubility of clary sage oil at 20○C reaches a significant value (0.094% for primary one, 0.406% for secondary one), but with an increase in temperature up to 40○C it decreases to 17-15%. Further study of the GLC data shows that the change in the percentage is due to an increase in the content of cineole and unidentified components in the oil, which have a relatively high solubility, hardly noticeable in the primary oil and accumulate in the secondary one and in the one isolated from the primary one. In the sample isolated from the water solution of the secondary oil there are 2 unidentified components located between cineole and linalool and apparently having increased solubility. Due to this, the percentage of linalool in the oil isolated from the solution decreases, but the absolute amount of the latter in 100 ml of the solution increases 2,58 times compared to the oil isolated from the primary oil solution.

Multifactorial Evaluation of Relaxing Properties of Juniperus virginiana L. Essential Oil and Comparison with Salvia sclarea L. Essential Oil

Virginian cedarwood (Juniperus virginiana L.) essential oil is an important component of a very large number of fragrances, used in cosmetics or household products. Clary sage (Salvia sclarea L.) essential oil is well known in aromatherapy for its sedative property. The essential oils of Virginian cedarwood’s crushed barks and clary sage’s leaves were analyzed by GC/MS. Respectively, seven and seventeen compounds; representing 78% and 94.13% of the essential oils, were identified. The main components of the cedar oil are cedrene alpha (25%) and cedrol (20%). The main components of the clary sage oil are linalyl acetate (69.4%) and linalool (11.9%). Cedarwood oil exhibits significant relaxing activity, influencing physiological and biochemical parameters in human. This shows repercussions on situational stress. Virginian Cedarwood essential oil activity is compared with clary sage essential oil, which proved to be less efficient on stress relief.

Thyme essential oil as an antimicrobial and biofilm inhibitory agent against abscesses with P. mirabilis Infections

IntroductionAbscesses are high density microbial infections of which biofilm formation plays a crucial role on their recurrence and chronicity. In this study, the authors aim was to determine biofilm formations of three different abscess cultures and to investigate antimicrobial and antibiofilm effects of clary sage, clove and thyme oils against the identified bacterial strain isolated from the highest biofilm forming abscess culture. MethodsThis in vitro study consisted of skin, breast and perianal abscess materials of 3 different patients. “Papanicolaou” staining and “Periodic Acid Schiff” staining methods were performed for cytological examination. Biofilm formation and biofilm inhibition tests were determined by “Crystal Violet Binding” assay and Minimum Inhibitory Concentrations (MIC) of the selected essential oils were calculated by “Micro-Dilution” method. ResultsEscherichia coli, Enterobacter aerogenes and Proteus mirabilis strains were isolated from perianal, skin and breast abscesses respectively. The bacterial strain that exists in the highest biofilm forming abscess culture was identified as P. mirabilis. P. mirabilis strain was found as resistant to Ampicillin and Trimethoprim/Sulfamethoxazole. Clary sage oil was unable to inhibit growth of P. mirabilis strain at the highest concentration tested (500 μL/mL). However, clove and thyme oils inhibited biofilm formation of P. mirabilis strain in addition to inhibiting its growth at very low concentrations (1.95 μL/mL and 0.98 μL/mL respectively). ConclusionThyme oil having the lowest inhibitory concentration and the highest antibiofilm effect has a good potential to be used as an antimicrobial and antibiofilm agent for the management of abscesses with P. mirabilis infections.

Inhalation of Clary Sage Oil before Chemotherapy Alleviates Anxiety and Stress without Changing Blood Pressure: A Randomized Controlled Trial

Inhalation of Clary Sage Oil before Chemotherapy Alleviates Anxiety and Stress without Changing Blood Pressure: A Randomized Controlled Trial

Effect of clary sage oil as an aromatherapy on cardiac autonomic function among patients with premenstrual syndrome – A randomized controlled study

BackgroundPremenstrual syndrome (PMS) is common among women in their reproductive age across the world and complementary therapies are being widely used in the management of PMS. Aromatherapy is one such complementary therapy and the objective of the current study is to evaluate its immediate effect on cardiac autonomic function among patients with PMS. MethodologyA total of 60 women with PMS were randomly allocated to either intervention group (clary sage aroma exposure; n = 30) or control group (water exposure; n = 30). Aroma exposure with aroma oil or water was given using aroma care diffuser. Short term HRV was measured at baseline and 10, and 20 min after inhalation. ResultsImmediately after aroma exposure, the time domain parameters of short term HRV showed a significant (P < 0.05) improvement towards the parasympathetic dominance after the recovery phase. Frequency domain parameters also showed the same direction of changes. In contrast, control group subjects did not show any changes in the time and frequency domain parameters. ConclusionThe present study showed that inhalation of clary oil aromatherapy act as a potential therapeutic modality to improves the autonomic function towards vagal tone domination. Further studies with longer duration of aroma inhalations are required to warrant our finding.

Clary sage essential oil and its biological activities

Salvia sclarea or “clary sage” is an aromatic plant that is used for various ailments especially among the women. The aim of this investigation was to evaluate the biological activities of clary sage oil with emphasis on its application in women health. The information of this study was extracted by key words of clary sage, S. sclarea, biological activities in references books and electronic resources. The results of investigation exhibited that the chemical composition of clary sage influenced from various ecological, geographical and environmental conditions, but linalool and linalyl acetate is known as main components of clary sage oil. Clary sage oil is used in aromatherapy for women during the menstrual cycle, childbirth and menopause. The modern clinical investigations confirmed the efficacy of clary sage oil as anti-stress, anti-depression and analgesic agents in primary dysmenorrhea. The anti-oxidant, antimicrobial, cytotoxic and anti-inflammatory effects of clary sage have been confirmed. Furthermore, clary sage oil is known as safe oil with no adverse effects. Designing the large clinical study to evaluate the oral efficacy of clary sage oil for women ailments is essential for future studies.

Effect of essential oil vapours on aflatoxin production ofAspergillus parasiticus

The effect of cinnamon, clary sage, juniper, lemon and marjoram essential oil (EO) vapours was tested on growth, aflatoxin production and sporulation ofAspergillus parasiticus. In reversed Petri-dish method the sub-lethal EO vapour concentrations ranging from 0.05 to 0.42 mg/cm3 air were used and growth rates (mm/day) and antifungal indices (%) were calculated from the growth curves of the fungus. Aflatoxin production was determined by HPLC and spores were counted in a Burker chamber. Cinnamon, clary sage and marjoram EOs showed concentration dependent growth inhibition. Antifungal index and aflatoxin production using the weak antifungals, juniper and lemon EO, increased in parallel. The same trend was found using cinnamon and clary sage EO vapours up to 0.11 mg/cm3 concentration, and marjoram EO up to 0.21 mg/cm3, while higher concentrations caused a sharp decrease in aflatoxin production. Applying sub-lethal concentrations of EOs might induce stress response inA. parasiticus leading to increased aflatoxin production. Only EO concentrations with strong growth and sporulation inhibitory effect were suitable to inhibit the aflatoxin production ofA. parasiticus..

Toxicity and Repellent Activity of Plant Essential Oils and Their Blending Effects Against Two Spotted Spider Mites, Tetranychus urticae Koch

Miticidal and repellent activity of twenty plant essential oils against the adults of two spotted spider mites, Tetranychus urticae, were examined. Sandal wood oil was the most potent one in mortality, whereas clary sage oil exhibited the greatest repellent activity. On those twenty essential oils tested, no apparent correlation between toxicity and repellency was observed. When the most active oils were blended, the combinations tend to exhibit antagonistic interactions in both toxicity and repellent activity. The chemical compositions of sandal wood oil and clary sage oil were identified via GC/MS analyses, and the major constituents of sandal wood oils were sesquiterpene compounds, whereas the major ones for clary sage oil were monoterpenes. Among the major components in clary sage oil, linalyl acetate was not only the most abundant constituent, but also the most responsible one for its repellent activity.

Effect of Antibacterial Plant Extracts on the Morphology of Electrospun Poly(Lactic Acid) Fibres.

Essential oils (EOs) of clary sage and black pepper induce changes in the morphology of poly(lactic acid) (PLA) electrospun fibres. The chemical composition of the oils is analysed by gas chromatography-mass spectrometry and Fourier-transform infrared spectroscopy; while the evaporation rate of the EOs and their main chemical components is characterised by Thermogravimetric Analysis. The addition of EOs generate thermodynamic instabilities during the electrospinning process, leading to the formation of fibres with either wrinkled (for clary sage oil) or nano-textured surfaces (for black pepper oil). The morphology of the PLA-EOs fibres is investigated by Scanning Electron Microscopy. Together with a well-defined structure, the fibres produced also possess antibacterial activity, as demonstrated by viability loss tests conducted on E. coli and S. epidermidis. Bacteria inactivation efficiency of 76 and 100% is reported for the composite PLA/essential oils electrospun mats. The composite mats produced are promising in the biomedical field, where nanotopography offers physical cues to regulate cell behaviour, and the delivery of therapeutic compounds (essential oils) limits microbial growth.

Mille modis morimur: We die in a thousand ways.

Dying cells subjected to apoptotic programs are engulfed by neighboring cells or by professional phagocytes, without inflammation or immunological reactions in the tissue where apoptosis takes place. Apoptotic cells release danger-associated project signals to their neighbours, through different molecular patterns, stimulate antigen production and immune responses. Microenvironmental effects with several functional consequences indicate that cell death is a complex process and may take place in several ways. This idea is expressed by the title of the Special Issue and by the title of the guest editorial "Mille modis morimur" meaning that not only multicellular organisms, but also single cells may die in a thousand ways. This idea is demonstrated by the papers serving as examples for cell death. Apoptosis was induced by clary sage oil in Candida cells. Heavy metal (Gd) induced cell motility and apoptosis was found in mammalian cells. RNA oxidation enhanced the reversion frequency of apoptosis in yeast mutants. The frequency of apoptotic micronucleus formation increased in a concentration-dependent manner by methotrexate. The antioxidant coenzyme Q10 protected renal proximal tubule cells against nicotine-induced apoptosis. The synergy of 2-deoxy-D-glucose combined with berberine induced lysosome/autophagy. The mitochondrial apoptotic pathway could be regulated by glucocorticoid receptor in collaboration with Bcl-2 family proteins in developing T cells. Cylindrospermopsin induced biochemical changes led to apoptosis in plants. Mechanisms of stress seriously impacted the risk of apoptosis. Transcriptional control of apoptotic cell clearance was achieved by macrophage nuclear receptors. Finally, the clinical aspects of apoptosis-induced lymphopenia were reviewed in sepsis and other severe injuries. These examples not only support the view of many ways of cell death, but predict further potential ways to induce or reduce the risk of cell death.

Effects of clary sage oil and its main components, linalool and linalyl acetate, on the plasma membrane of Candida albicans: an in vivo EPR study.

The effects of clary sage (Salvia sclarea L.) oil (CS-oil), and its two main components, linalool (Lol) and linalyl acetate (LA), on cells of the eukaryotic human pathogen yeast Candida albicans were studied. Dynamic and thermodynamic properties of the plasma membrane were investigated by electron paramagnetic resonance (EPR) spectroscopy, with 5-doxylstearic acid (5-SASL) and 16-SASL as spin labels. The monitoring of the head group regions with 5-SASL revealed break-point frequency decrease in a temperature dependent manner of the plasma membrane between 9.55 and 13.15 °C in untreated, in CS-oil-, Lol- and LA-treated membranes. The results suggest a significant increase in fluidity of the treated plasma membranes close to the head groups. Comparison of the results observed with the two spin labels demonstrated that CS-oil and LA induced an increased level of fluidization at both depths of the plasma membrane. Whereas Lol treatment induced a less (1 %) ordered bilayer organization in the superficial regions and an increased (10 %) order of the membrane leaflet in deeper layers. Acute toxicity tests and EPR results indicated that both the apoptotic and the effects exerted on the plasma membrane fluidity depended on the composition and chemical structure of the examined materials. In comparison with the control, treatment with CS-oil, Lol or LA induced 13.0, 12.3 and 26.4 % loss respectively, of the metabolites absorbing at 260nm, as a biological consequence of the plasma membrane fluidizing effects. Our results confirmed that clary sage oil causes plasma membrane perturbations which leads to cell apoptosis process.

The effect of clary sage oil on staphylococci responsible for wound infections.

IntroductionThe spreading of bacterial antibiotic resistance among clinical strains of pathogenic bacteria has made investigators to search for other active antibacterial agents which could provide a valuable complement to the existing therapies.AimTo determine the antibacterial activity of clary sage oil (Salvia sclarea L.) against Staphylococcus clinical strains which were isolated from patients with wound infections.Material and methodsA comprehensive evaluation of Staphylococcus clinical strain resistance to antibiotics was performed. The constituents of clary sage oil were assayed by GC-FID-MS analysis. The minimal inhibitory concentration (MIC) of the tested essential oil against staphylococci by the micro-dilution broth method was determined.ResultsThe clary sage oil was active against Staphylococcus aureus, S. epidermidis and S. xylosus with MIC values ranging from 3.75 to 7.00 µl/ml.ConclusionsThe results of the in vitro tests encourage to use formulations containing sage oil as the active natural antimicrobial agent. Because of its antimicrobial properties clary sage oil may be applied to treat wounds and skin infections.

Simultaneous Determination of Linalool, Ambrox, and Sclareolide in Clary Sage Oil by GC-MS

Abstract The volatile components of clary sage oil were identified by gas chromatography/time-of-flight mass spectrometry (GC-TOFMS). The peak area normalization method was used to calculate the relative content of the components. With the MS library search, 32 compounds, which account for 91.24% in the volatile components of clary sage oil, were confirmed by using retention index. A rapid and effective GC-MS method for simultaneous determination of linalool, ambrox, and sclareolide in clary sage oil has been developed. Three samples were used for method evaluation. The relative standard deviations (RSD) were &amp;lt;4% for linalool, ambrox, and sclareolide. The limits of detections (LOD) were 80, 50, and 150 µg L−1 for linalool, ambrox, and sclareolide, respectively. The recoveries were 90–109% for linalool, 91–105% for ambrox, and 94–103% for sclareolide. The method could be applied to measure linalool, ambrox, and sclareolide in clary sage oil.

Changes in 5‐hydroxytryptamine and Cortisol Plasma Levels in Menopausal Women After Inhalation of Clary Sage Oil

Changes in 5‐hydroxytryptamine and Cortisol Plasma Levels in Menopausal Women After Inhalation of Clary Sage Oil

Effects of Salvia sclarea on chronic immobilization stress induced endothelial dysfunction in rats.

BackgroundAlthough Salvia sclarea (clary sage) is widely used in aromatherapy and has antioxidant and antimicrobial properties, its mechanisms of action remain poorly understood. We therefore assessed whether clary sage is effective in treating endothelial dysfunction induced by chronic immobilization stress in rats.MethodsRats were intraperitoneally injected with almond oil, clary sage oil (5%, 10% or 20%), or nifedipine once daily, followed by immobilization stress (2 h/day) for 14 days. Systolic blood pressure (SBP) and heart rate (HR) were measured, as were serum concentrations of corticosterone (CORT); a biomarker of chronic stress, malondialdehyde (MDA); a biomarker of oxidative stress. Nitric oxide production was assessed by nitrite assays, and eNOS level, a biomarker of endothelial dysfunction, was measured by western blotting. Endothelial dysfunction was also assayed by measuring the effect of clary sage on the contraction of rat aortae.ResultsTreatment with 5% (p = 0.029), 10% (p = 0.008), and 20% (p = 0.008) clary sage significantly reduced SBP and treatment with 20% clary sage significantly reduced HR (p = 0.039) compared with the chronic immobilization stress group. Clary sage decreased CORT serum concentration (10%, p = 0.026; 20%, p = 0.012) and MDA (10%, p = 0.007; 20%, p = 0.027), findings similar to those observed with nifedipine. In addition, 20% clary sage significantly increased nitric oxide production (p <0.001) and eNOS expression level (p <0.001) and relaxed aortic rings in rats subjected to chronic immobilization stress.ConclusionsClary sage treatment of rats subjected to immobilization stress contributed to their recovery from endothelial dysfunction by increasing NO production and eNOS level as well as by decreasing oxidative stress. Appropriate concentration of clary sage may result in recovery from endothelial dysfunction. These findings indicate that clary sage oil may be effective in the prevention and treatment of stress-induced cardiovascular diseases.

Changes in 5-hydroxytryptamine and cortisol plasma levels in menopausal women after inhalation of clary sage oil.

The purpose of this study was to examine the antidepressant-like effects of clary sage oil on human beings by comparing the neurotransmitter level change in plasma. The voluntary participants were 22 menopausal women in 50's. Subjects were classified into normal and depression tendency groups using each of Korean version of Beck Depression Inventory-I (KBDI-I), KBDI-II, and Korean version of Self-rating Depression Scale. Then, the changes in neurotransmitter concentrations were compared between two groups. After inhalation of clary sage oil, cortisol levels were significantly decreased while 5-hydroxytryptamine (5-HT) concentration was significantly increased. Thyroid stimulating hormone was also reduced in all groups but not statistically significantly. The different change rate of 5-HT concentration between normal and depression tendency groups was variable according to the depression measurement inventory. When using KBDI-I and KBDI-II, 5-HT increased by 341% and 828% for the normal group and 484% and 257% for the depression tendency group, respectively. The change rate of cortisol was greater in depression tendency groups compared with normal groups, and this difference was statistically significant when using KBDI-II (31% vs. 16% reduction) and Self-rating Depression Scale inventory (36% vs. 8.3% reduction). Among three inventories, only KBDI-II differentiated normal and depression tendency groups with significantly different cortisol level. Finally, clary sage oil has antidepressant-like effect, and KBDI-II inventory may be the most sensitive and valid tool in screening for depression status or severity.