Background We suggested that the rescue of the blood vessels may be essential for the stable gastric pentadecapeptide BPC 157 as therapy in ulcerative colitis (Curr Med Chem. 2012;19(1):126-32.; Curr Pharm Des. 2011;17(16):1612-32.). Here, we hypothesized that BPC 157 rescues blood vessels in intestinal ischemia injury caused by the portal triad clamping. Of note, depending about the ischemia attenuation or aggravation, the number of blood vessels due to more or less branching may correspondingly decreased (aggravated ischemia) or increase (attenuated ischemia, and rescued blood vessels maintenance), and likewise, anastomosis formation should additionally contribute decreased or increased number of blood vessels presented. A comparison was carried out with the effect of the L-NAME, and L-arginine given alone and/or together. Methods In deeply anaesthetised rats, throughout 30min of the portal triad clamping, in the minute intervals, the assessment involved the most distal part of the ileum, and caecum, 1 cm long, four neighboring vessels where the all arising branches including formed anastomosis between them were all assessed as the total blood vessels number. Besides, medication (/kg) as bath at the clamped area (BPC 157 (10μg, 10ng), L-NAME (5mg), L-arginine (100mg), alone and/or together, was applied at 5min after the portal triad clamping. Results/Discussion At the 30min of the portal triad clamping, controls were presented with fewer branching and anastomosis (intestinum 14.3±1.2; caecum 122.5±5.2), BPC 157 with more branching and anastomosis (intestinum 27.5±3.2; caecum 154.5±7.2, both P<0.05, at least vs. control), markedly less intestine congestion along with few microscopical findings (Figure 1). NO-system agents presented different results, L-arginine a decrease (intestinum 9.2±1.0; caecum 63.3±6.6, both P<0.05, at least vs. control); L-NAME an effect on caecum (intestinum 13.3±2.2; caecum 102.5±5.2, P<0.05, at least vs. control); L-NAME+L-arginine (intestinum 6.3±1.0; caecum 147.5±5.2 both P<0.05, at least vs. control) suggest that these effects are at least partly NO-system related; BPC 157 overcomes the effect of L-arginine (BPC 157+L-arginine (intestinum 33.3±2.2; caecum 183.5±5.2 both P<0.05, at least vs. control); BPC 157+L-arginine+LNAME (intestinum 21.8±2.2; caecum 165.5±5.2 both P<0.05, at least vs. control); its effect was however counteracted by L-NAME: BPC 157+L-NAME (intestinum 14.5±2.2; caecum 106.5±5.2). Conclusions BPC 157 could be used along with portal triad clamping, and may rescue blood vessels in both intestine and caecum, an effect at least partly related to NO-system.
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