Calamondin Research Articles

Citrus microcarpa Biochar: A Green Solution for the Adsorption of Dyes and Phenols

Citrus microcarpa Biochar: A Green Solution for the Adsorption of Dyes and Phenols

Immunomodulatory Effects of Citrus Species Ethanol Extracts on Cellular Immune Responses in Wistar Rats and Mice

Currenty, Citrus reticulata has been found to modulate RAW 264.7 macrophage cell line. In the present study, the immunomodulatory effects of Citrus species peel extracts, including Citrus microcarpa, C. limon L., C. sinensis L. and C. hystrix were investigated on cellular immune responses in Wistar rats and mice. Mice were treated with ethanol extracts of Citrus species orally at dosages of 25, 50 and 100 mg/kg bw for 7 days. The phagocytosis activity of the extracts was examined using the carbon clearance method. Meanwhile, oral administration of Citrus species ethanol extracts at dosages of 17.5, 35 and 70 mg/kg bw was administered to Wistar rats to assess the effect of extracts on the delayed-type hypersensitivity (DTH) response using paw edema-method. Thin Layer Chromatography (TLC)-densitometry analysis was also performed. In both animal models, all extracts enhanced the cellular immune responses. Amongst the samples, C. sinensis, at the dose of 100 mg/kg bw in mice and 70 mg/kb bw in rats, demonstrated the highest stimulation on phagocytosis activity and DTH response, respectively. Except C. microcarpa, rutin was found in all Citrus species. The results indicate that Citrus species, particularly C. sinensis, have the potential to be developed into a potent immunostimulatory agent. HIGHLIGHTS The immunomodulatory effects Citrus species were investigated using mice and rats as animal models. Citrus microcarpa, C. limon, C. sinensis L. and C. hystrix were able to stimulate phagocytosis in mice and delayed-type hypersensitivity response in rats. Citrus sinensis demonstrated the highest stimulation on phagocytosis activity and delayed-type hypersensitivity response. Rutin was found in all Citrus species, except in microcarpa peels extract. GRAPHICAL ABSTRACT

The effectiveness of calamansi (Citrus microcarpa) extract in enhancing the shelf life and quality of dakgalbi made from domestic Korean chicken

The effectiveness of calamansi (Citrus microcarpa) extract in enhancing the shelf life and quality of dakgalbi made from domestic Korean chicken

Involvement of CgHSFB1 in the regulation of self-incompatibility in 'Shatian' pummelo.

As self-incompatibility is a major issue in pummelo breeding and production, its mechanism in citrus was analyzed to improve breeding efficiency and reduce production costs. Rutaceae belongs to S-RNase type of gametophytic self-incompatibility. While the function of S-RNase/SLF and the mechanism of self-incompatibility have been studied extensively, the transcriptional regulation of S-RNase has been less studied. We performed transcriptome sequencing with the styles of 'Shatian' pummelo on the day of anthesis and 1-5days before anthesis, and found that the transcript level of S-RNase gradually decreased with flower development. By analyzing differentially expressed genes and correlation with the expression trend of S-RNase, we identified a candidate gene, CgHSFB1, and utilized biochemical experiments such as yeast one-hybrid assay, electrophoretic mobility shift assay and dual-luciferase assay, as well as transient transformation of citrus calli and Citrus microcarpa and demonstrated that CgHSFB1 could directly bind to the S1-RNase promoter and repress the expression of S1-RNase, which is involved in the pummelo self-incompatibility response. In contrast, CgHSFB1 did not bind to the promoter of S2-RNase, and there was specificity in the regulation of S-RNase.

Comparative Antibacterial Effectiveness of Orange Peel Extracts from Five Varieties Against Strain of Multi Drug Resistance Escherichia coli

The purpose of this study was to investigate the antibacterial activity of orange peel extracts from five different orange varieties in North Sumatera against resistant Escherichia coli bacteria and compare it to the effectiveness of vancomycin and erythromycin. To assess antibacterial activity, orange peel extracts were prepared from five different orange varieties, namely Citrus aurantifolia, Citrus microcarpa Bunge, Citrus limon L., Citrus sinensis L., and Citrus hystrix. Antibacterial activities were evaluated using the microdilution method to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The best antibacterial activity was determined using the smallest MIC and MBC values. The research found that all five orange peel extracts exhibited significant antibacterial activity compared to the standard antibiotics vancomycin and erythromycin. The most potent inhibitory effect was observed with the Citrus hystrix peel extract, with the lowest MIC and MBC (125 and 250 µg/mL), followed by Citrus aurantifolia (250 and 500 µg/mL), and Citrus sinensis L. (500 and 500 µg/mL). The results showed significant antibacterial activity of orange peel extracts from five different orange varieties in North Sumatera against resistant Escherichia coli bacteria. Keywords: Antibiotic resistance, citrus peel extracts, Escherichia coli, MIC, MBC.

Development and characterization of butterfly pea (Clitoria ternatea L.) flower juice from spray dried powder

This study intended to use Butterfly pea (Clitoria ternatea L.) double petaloid in producing a powder used in formulating a stable, nutritious and antioxidant rich juice. The butterfly pea powder (BPP) was produced through spray drying and it had a recovery of 87.8%. The antioxidant activity of BPP ranged from 52.1 to 61.9% and its anthocyanin content was 24.0 mg/L. The physico-chemical analysis of the BPP obtained a mean pH of 4.86, %TSS value of 29.2% and mean %TA of 0.023%. The proximate composition of BPP was 0.08% moisture content, 0.004% crude fat, 0.003% crude protein, 0.83% crude fiber, 0.58% crude ash and a total of 98.5% carbohydrates. Three formulations were used in the production of Butterfly pea juice (BPJ) with varying BPP content as follows: 0.01 g BPP/ml (BPJT1), 0.02 g BPP/ml (BPJT2) and 0.03 g/ml of water (BPJT3). The constant amounts of calamansi (Citrofortunella microcarpa) and sugar were used for all three formulations. It was observed that BPJT3 is the most preferred formulation. Kramer's test performed at the 5% level of significance showed a significant difference between the BPJ treatments. As for the overall acceptability of the three formulations, BPJT1 and BPJT2 were “liked moderately” while BPJT3 was "liked much". The Kruskal-Wallis test at α=0.05 showed no significant difference between the general acceptability of the formulas with a P-value of 0.099. The antioxidant activity of BPJ ranged from 46.4% to 50.1% and its anthocyanin content was 18.6 mg/L. The physicochemical analysis of butterfly pea juice (BPJ) obtained an average pH of 2.95, a %TSS value of 10.4% and a mean %TA of 0.029%.

Activity of the Ethanolic Extract Obtained from Citrus microcarpa Pericarps against Meloidogyne enterolobii, and Chemical Composition Analysis

Citrus microcarpa is a popular nutritious fruit that is widely cultivated in China. In recent years, many compounds with significant pharmacological activities have been isolated successfully from the pericarp of C. microcarpa. However, to date, there are no reports on the activity of C. microcarpa pericarp against root-knot nematodes. This study used the ethanolic extract from the pericarp of Hainan C. microcarpa and the impregnation method to determine its activity on J2 Meloidogyne enterolobii specimens and on single-egg hatching. The results showed that when J2 individuals were treated with 50 mg⋅mL–1 of the extract, the lethal concentration 50 values after 24 and 48 hours were 17.124 and 8.858 mg⋅mL–1, respectively. The mortality rate of nematodes after 48 hours of treatment was 100%, and the inhibition rate of single-egg hatching after 24 hours was 89.29%. The ethanolic extract of C. microcarpa peels showed high inhibitory and lethal activity against the M. enterolobii. The analysis of the chemical composition of the extract revealed 28 substances with insecticidal and antibacterial effects, including lignans, flavonoids, fatty acids, organic acids, terpenoids, and imidazole. The formulas of the chemical structures and pharmacological effects of these potential insecticidal and antibacterial substances were elucidated to provide a scientific basis and a theoretical reference for the use of C. microcarpa pericarps as a raw material for the development of new, natural plant nematicides.

Cross-Inoculation of Commercial Forestry, Amenity, and Horticulture Tree Species with Ceratocystis Isolates Collected from Different Host Species.

Ceratocystis manginecans has caused significant losses in forestry productivity in Indonesia and neighboring nations. It also infects horticultural trees, but the host range of individual isolates of C. manginecans is poorly studied. So, this study aimed to better understand the potential host range and evaluate aggressiveness against forestry and fruit tree species of C. manginecans isolated from various tree species in Indonesia. Five C. manginecans isolates, four from different tree species and one from the shot-hole borer Euwallacea perbrevis, were used to inoculate seven fruit and six forest tree species, including E. pellita and Acacia mangium. Many of the inoculated trees produced typical canker disease symptoms, such as rough, swollen, and cracked lesions on the bark, but some trees did not have any external symptoms. Mortality in the most susceptible clone of A. mangium was 40% within 8 weeks. Forest tree species were more susceptible than fruit trees, with the length of xylem discoloration ranging from 0.4 to 101 cm. In fruit trees, the average extent of xylem discoloration was lower, ranging from 0.4 to 20.5 cm; however, mortalities were recorded in two fruit tree species, Citrus microcarpa and Durio zibethinus. Host-isolate interaction was evident; isolate Ep106C from Eucalyptus pellita caused the greatest xylem discoloration in Citrus sp., whereas Hy163C from Hymenaea courbaril was the most damaging in D. zibethinus, Artocarpus heterophyllus, and Mangifera indica. Increasingly globalized food and fiber systems increase risk of disease spread, and the serious threat of C. manginecans incursions into countries where it is not present must be evaluated more thoroughly.

Aphid species found on citrus in Ogan Komering Ilir District, South Sumatra, Indonesia

Citrus is an economically valuable plantation crop and has high market opportunities. In Southeast Asia, Indonesia is the second largest citrus importer after Malaysia, with an import volume of 160,254 tons; however, various obstacles limiting the cultivation of citrus plants cannot be separated from pests that can affect citrus quality and reduce citrus production. One of the obstacles is insect attacks such as aphids. Therefore, this study aims to determine the species of aphids in citrus. This research was conducted from May to June 2023 in Ogan Komering Ilir Regency using a direct observation method in the field. Observations were made on the parts attacked by aphids and symptoms of attack. The results showed that three species from 1 family of aphids were found. The species found were Toxoptera aurantii, Toxoptera citricidus, and Toxoptera citricola from the Aphididae family. The highest species found in the study was T. aurantii, with 861 individuals, and the lowest species found was T. citricola, with 29 individuals. Aphid species were found mainly on Citrus microcarpa and least on Citrus grandis.

KASTURI ORANGE PEEL (CITRUS MICROCARPA BUNGE) ESSENTIAL OIL: CHEMICAL PROFILE, FORMULATION AS ROLL-ON AROMATHERAPY AND ITS EVALUATION

Objective: Kasturi orange (Citrus microcarpa Bunge) is widely cultivated and consumed in Indonesia. The fruit's flesh is a good source of vitamin C. Essential oil of kasturi orange peel is one of the ingredients used as a raw material for aromatherapy roll-on preparations. Roll-on aromatherapy is currently widely used by various age groups. This research aims to determine the content of kasturi oil, its formulation as a roll-on aromatherapy preparation, and its physical evaluation.
 Methods: The essential oil of kasturi orange peel was extracted using the distillation method, and then the physicochemical properties were examined. The chemical content of the oil was analyzed using GC-MS. The roll-on aromatherapy preparation formulas were made by varying concentrations of kasturi oil, F0 (0%), F1 (4%), F2 (6%), and F3 (10%). Additional substances used were menthol, camphor, patchouli oil, and virgin coconut oil. Patchouli oil was used in this preparation to make the aroma last longer. Evaluation of roll-on aromatherapy preparations includes organoleptic tests, pH tests, specific gravity, viscosity, clarity, and stability.
 Results: From the extraction results, the percentage yield of essential kasturi oil was 0.55% v/w, with a density of 0.85 g/ml and a refractive index of 1.469. From the chromatogram results, there were 18 compounds in kasturi oil; the largest component was D-limonene (32.59%). Physical evaluation results of all roll-on aromatherapy formulas had met the requirements.
 Conclusion: From the chromatogram results, there were 18 compounds in kasturi oil, with the largest component were D-limonene (32.59%). Formula F3 had the best aroma intensity, approximately for 5 h, with a slightly strong aroma, and physical evaluation results of all roll-on aromatherapy formulas had met the requirements.

Essential oil from Citrus depressa peel exhibits antimicrobial, antioxidant and cancer chemopreventive effects.

Many diseases may be caused by pathogens and oxidative stress resulting from carcinogens. Earlier studies have highlighted the antimicrobial and antioxidant effects of plant essential oils (EO). It is crucial to effectively utilize agricultural waste to achieve a sustainable agricultural economy and protect the environment. The present study aimed to evaluate the potential benefits of EO extracted from the discarded peels of Citrus depressa Hayata (CD) and Citrus microcarpa Bunge (CM), synonyms of Citrus deliciosa Ten and Citrus japonica Thunb, respectively. Gas chromatography-mass spectrometry analysis revealed that the main compounds in CD-EO were (R)-(+)-limonene (38.97%), γ-terpinene (24.39%) and linalool (6.22%), whereas, in CM-EO, the main compounds were (R)-(+)-limonene (48.00%), β-pinene (13.60%) and γ-terpinene (12.07%). CD-EO exhibited inhibitory effects on the growth of common microorganisms, including Candida albicans, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. However, CM-EO showed only inhibitory effects on E. coli. Furthermore, CD-EO exhibited superior antioxidant potential, as demonstrated by its ability to eliminate 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azinobis-3-ethylbenzthiazoline-6-sulfonate free radicals. Furthermore, CD-EO at a concentration of 100 μg mL-1 significantly inhibited 12-O-tetradecanoylphorbol-13-acetate-induced cancer transformation in mouse epidermal JB6 P+ cells (P < 0.05), possibly by up-regulating protein expression of nuclear factor erythroid 2-related factor 2 and its downstream antioxidant enzymes, such as NAD(P)H:quinone oxidoreductase 1, heme oxygenase-1 and UGT1A. These findings suggest that CD-EO exhibits inhibitory effects on pathogenic microorganisms, possesses antioxidant properties and has cancer chemopreventive potential. © 2024 Society of Chemical Industry.

Inventory of the Citrus in Barimbun Village, Tabalong District

Indonesia is rich in flora diversity, especially fruit plants. One of fruit plants that is familiar to Indonesian people is the orange tree. Orange trees are annual plants originating from the Asian continent, especially India to China. In Indonesia, orange plants are diverse and have unique characteristics in the form of aroma, color and taste that are different from other fruits. The aim of this research is to describe the diversity of citrus plants (genus Citrus) in Barimbun Village, Tabalong. This research uses the line transect method. The research results found 7 types of oranges, namely, Citrus microcarpa, Citrus jambhiri, Citrus aurantifolia, Citrus gandis, Citrus hystrix, Citrus sinensis, and Citrus limon. Moreover, the abiotic factors give good value which means a suitable habitat for oranges. From this study, it may conclude that Barimbun Village, Tabalong District is a suitable habitat for orange plant because this area has enough amount of rainfall and stabil temperature.

Training On Making Roll-On Aromatherapy Of Calamansi Orange Essential Oil In Pondok Kubang Sub-District, Central Bengkulu

Calamansi orange (Citrofortunella microcarpa) is one of the commodities that has been developed and processed into calamansi orange syrup. One of the by-products of the calamansi orange syrup industry is peel. Calamansi orange peel contains a lot of essential oils, the use of which can be made in the form of aromatherapy roll-on preparations. One of the essential oil contents of calamansi orange is decanal. Decanal in aromatherapy causes a feeling of relaxation. The aim of this service is to train the community on how to make calamansi orange essential oil aromatherapy in roll on packaging form at MSMEs Bukit Bengkulu, Pondok Kubang Sub-District, Central Bengkulu. The community service method is in the form of training on making essential oil from Calamansi orange peel and making preparations in the form of roll-on for aromatherapy using leaflets, tools and materials that have been prepared, the number of participants is 15 peoples. The service team carried out a demonstration and then were given the opportunity to demonstrate one by one. The results of this training show that the participants have the skills to make calamansi essential oil with an average score of 83 and have the skills to make roll on essential oil for calamansi orange peel, namely an average score of 87, exceeding the target of 80. The results of this training show an increase in participants’s skills in making essential oils and calamansi orange peel aromatherapy roll-on which can be used as aromatherapy and increase community income through MSMEs.

Antibacterial, bacteriolytic, antibiofilm, and synergistic effects of the peel oils of Citrus microcarpa and Citrus x amblycarpa with tetracycline against foodborne Escherichia coli.

Citrus essential oils (EOs) have shown significant antibacterial activity. The present study was undertaken to evaluate the antibacterial activity of the peel oils of Citrus microcarpa and C. x amblycarpa against Escherichia coli. The minimum inhibition concentration (MIC) was determined by using the broth microdilution assay. The checkerboard method was used to identify synergistic effects of the EOs with tetracycline, while bacteriolysis was assessed by calculating the optical density of the bacterial supernatant, crystal violet assay was used to assess their antibiofilm. Ethidium bromide accumulation test was employed to assess efflux pump inhibition. Electron microscope analysis was performed to observe its morphological changes. The EOs of C. microcarpa and C. x amblycarpa were found to contain D-limonene major compound at 55.78% and 46.7%, respectively. Citrus microcarpa EOs exhibited moderate antibacterial against E. coli with a MIC value of 200 μg/mL. The combination of C. microcarpa oil (7.8 μg/mL) and tetracycline (62.5 μg/mL) exhibited a synergy with FICI of 0.5. This combination inhibited biofilm formation and disrupt bacterial cell membranes. Citrus microcarpa EOs blocked the efflux pumps in E. coli. Citrus microcarpa EOs demonstrated promising antibacterial activity, which can be further explored for the development of drugs to combat E. coli.

Effect of dextran and concentrated fructose from Weissella cibaria in the physicochemical and sensory properties of milk bread (pan de leche), gelato and calamansi (Citrus microcarpa) juice

SummaryAn exopolysaccharide‐producing isolate, Weissella cibaria ITDI 0199, was investigated to determine its ability to produce dextran and fructose, and its applicability to various food products. The isolate was cultivated in a fermentation medium of sucrose‐enriched coconut water waste obtained from a virgin coconut oil‐producing plant. Dextran produced from W. cibaria ITDI 0199 was processed by spray‐drying with maltodextrin and was incorporated into milk bread and gelato ice cream. At the same time, concentrated fructose was utilised as a partial substitute ingredient for ready‐to‐drink (RTD) calamansi (Citrus microcarpa) juice. Incorporating spray‐dried dextran into bread showed reduced cooking time, possible lower labour, and mechanical input costs, while the gelato products exhibited an increase in viscosity and thickness of ice cream mixture, leading to a decrease in ice cream melting rate when exposed at room temperature (25 °C). Additionally, using fructose as a sweetener in the calamansi juice reduced dependence on sugar. It has increased the dilution factor of the final product, potentially leading to increased profits.

Antioxidant and lipid peroxidation inhibitory potentials of the unripe and ripe of Citrus mitis Blanco pulps and peels from south Thailand

Citrus mitis Blanco fruit contains high levels of vitamin C, phenolics, and flavonoids, which exhibit a wide range of beneficial biological effects. The current research aimed to evaluate the levels of vitamin C, flavonoid, total phenolic contents, and antioxidant properties, as well as the inhibitory effects on lipid peroxidation, in both unripe and ripe fruit tissues (pulps and peels) of C. mitis Blanco. The fruit tissues were obtained from the Hat Som Paen subdistrict in Ranong province, located in the southern region of Thailand. Fruit tissues were extracted using maceration with 80% ethanol and water solvents. Vitamin C quantification was achieved using the 2,6-dichloroindophenol titrimetric method. Total flavonoid content was determined using the aluminium chloride colorimetric method, while total phenolic content was assessed using the Folin-Ciocalteu procedure. Additionally, ABTS and DPPH assays were used to assess the antioxidant properties. The measurement of lipid peroxidation inhibitory activity was carried out using the thiobarbituric acid reactive substances (TBARS) assay. The findings indicated that the water extract exhibited higher levels of vitamin C compared to the 80% ethanolic extract. In contrast, the 80% ethanolic extract revealed greater amounts of total phenolic and flavonoid contents than the water extract. The water extract of unripe pulp exhibited the highest vitamin C content among all the extracts, reaching 36.74 mg/100 g fresh weight (FW). The 80% ethanolic extract of unripe peel demonstrated the highest level of total phenolic content (35.67 mg gallic acid equivalent (GAE)/g extract), while ripe peel exhibited the highest total flavonoid content (16.88 mg catechin equivalent (CE)/g extract). Both unripe and ripe fruit extracts exhibited antioxidant and lipid peroxidation inhibitory activities. The 80% ethanolic extract from unripe peel demonstrated the most significant antioxidant activities against DPPH (6.31 mg trolox equivalent (TE)/g extract) and ABTS (7.69 mg TE/g extract). Moreover, the 80% ethanolic extract of unripe peel showed the highest level of inhibitory activity against lipid peroxidation, with a value of 74.62%. These results underscore the potential bioactivities of unripe and ripe C. mitis Blanco fruit tissues, which can offer nutritional and health benefits. These properties make them particularly valuable in the food industry due to their excellent antioxidant and lipid peroxidation inhibitory characteristics.

Value chain-based development plan for the calamansi (Citrus microcarpa) industry

Calamansi, a staple condiment in Filipino cuisine, plays a pivotal role in the culinary heritage of the Philippines. The province of Nueva Ecija, situated in Central Luzon, has emerged as the primary producer of calamansi in the region. This study investigates the calamansi industry's dynamics in Nueva Ecija over a six-year period, encompassing 18 municipalities. A comprehensive analysis reveals that 756,136 calamansi trees are cultivated across 1,200 hectares of land, involving 1,481 farmers. Regrettably, both production and cultivation area have witnessed a declining trend during this period, with annual negative growth rates of -3.83% and -0.68%, respectively. Motivated by this concerning scenario, the researcher, a native of Nueva Ecija and a farmer, embarked on an agribusiness research endeavor. Employing a descriptive research approach and value chain analysis, this study scrutinizes the current state of the calamansi industry. Primary data were collected from 50 calamansi growers, while secondary sources included provincial, city, and municipal agriculturists from relevant local government units. The findings unveil key challenges faced by calamansi growers, including high input costs, price manipulation by intermediaries and traders, price fluctuations, and losses due to pest infestations and adverse weather conditions. To enhance competitiveness and ensure the industry's stability, collaborative efforts along the value chain are imperative. This study underscores the need for collective action to promote the sustainability of the calamansi industry in Nueva Ecija, highlighting opportunities for growth and development.

Molecular cytogenetic maps provide new insights into the differences between hybrid Citrus microcarpa and lemons

Citrus microcarpa is one of the most popular species for flavoring and industrialized juice production, making it an increasingly important economic crop. At present, C. microcarpa has been mistakenly regarded as a type of lemon in China. The lack of a simple identification method for hybrid C. microcarpa is the major obstacle limiting its comprehensive recognition and utilization. In this study, firstly nine pairs of chromosomes were marked by eight oligo pools (OPs) in C. microcarpa, C. aurantiifolia, and C. limon, which showed that oligonucleotide (oligo) sequences are highly conserved within the genomes of the citrus family, suggesting that eight OPs and nine chromosome painting probes (CPPs) can be used as universal probes for the identification of each chromosome in citrus plants. Then we developed a method for the identification of C. microcarpa by using fluorescence in situ hybridization (FISH) mapping and Chromoycin A3 (CMA) staining. Moreover, we constructed the molecular cytogenetic maps containing the physical positions of eight OPs, 45S rDNA, 5S rDNA, CL, CMA banding and fragile sites in C. microcarpa, C. aurantiifolia, and C. limon, which visualize the significant differences among the three Citrus species. Combining the highly different morphological and cytological characteristics of C. microcarpa with C. aurantiifolia and C. limon, it is clear that C. microcarpa is a Citrus hybrid species, not a lemon species. Overall, this study provides a better understanding of C. microcarpa from the molecular cytogenetic perspective.

Favorable Antitumor Activity of Citrus microcarpa B. on Human Colon Adenocarcinoma Tumor Xenografted in Immunosupressed Mice.

The antitumor activity of Citrus microcarpa B. on HT29 human colon adenocarcinoma tumors xenografted in immunosuppressed mice was determined in this study. The objective of the study was to determine if the crude extract of C. microcarpa B. exhibited antitumor activity against HT29 human colon adenocarcinoma tumors xenografted in immunosuppressed mice. Cyclosporine-induced immunosuppressed mice were injected subcutaneously with 106 HT29 cells in the caudo-dorsal area of the back near the base of the tail to induce tumor growth. Tumors were grown for 9 days, and the mice were then administered with C. microcarpa B. (160 and 630 mg/kg) (Group A; n = 4 and B; n = 4) and normal saline solution (Group C; n = 4) intraperitoneally. Tumor volume was measured to assess the change in tumor volume after 24, 48, and 72-hour post-treatment administration. Tumors were then excised and analyzed histopathologically to evaluate the ratio of necrotic area to viable cancer cells in the tumors. Treatment of C. microcarpa B. with a dose of 160 mg/kg (P=0.002) and 630 mg/kg produced a significant decrease in tumor volume with the significance only observed at 72 hours post-treatment. Histopathological analysis showed a considerable decrease in the area of necrosis against viable tumor cells in the treatment of C. microcarpa B. with a dose of 630 mg/kg. It can thus be said that C. microcarpa B. is effective in reducing tumor volume, specifically at a dose of 630 mg/kg 72-hours post-treatment.

Atsiri Oil Potential of Peel of Kaffir Lime, Lime, and Calamansi Orange as Antioxidant and Antibacteria

Orange is a member of the Rutaceae family which is known as one of the horticultural crops that is widely cultivated in the tropical and subtropical regions of southern Asia. Citrus plants such as fruit peels are used as a source of essential oil-producing ingredients that have antioxidant and antimicrobial activity. This study aims to determine the potential of kaffir lime, lime, and calamansi orange peel essential oils as antioxidants and antibacterials. Scoping review uses the PEOS framework and several databases such as the electronic database Atlantis Press, PubMed, ScienceDirect, and the Google Scholar search engine. Search results that meet the criteria are then analyzed using PRISMA Flowchart, Data Extraction, and Theme Mapping. The results of the scoping review study of 74 articles related to titles and abstracts, 8 articles met the inclusion and exclusion criteria. Essential oils from kaffir lime peel (Citrus hystrix), lime (Citrus aurantifolia), and calamansi orange (Citrus microcarpa Bunge.) have antioxidant and antibacterial activities. The higher the concentration of essential oils from the peel of the fruit, the greater the antioxidant and antibacterial activity produced. Fresh orange peel essential oil has the strongest antioxidant and antibacterial activity compared to orange twig and leaf oil.