Changes in fluid within the vitreous chamber were examined using a non-invasive technique using LED. The vitreous chamber was irradiated with a certain amount of LED as an elliptical circular sphere to confirm the acute-angle vitreous chamber status with continuous pulsation transition. Dazzling-divergence knowledge level (DDKL) was determined as the number generated by pulsation knowledge figuration by receiving the scattered signal generated from the irradiated LED for a certain number of seconds. The knowledge level was configured as a system that converts pulsation generated in a vitreous chamber, and the uneven conveyance technique was used to confirm the experimental conditions of dazzling halftone-free-vector-dot. The degree of scattering around micro-vessels and foreign substances was found to occur at a halftone-free-vector-dot level within the vitreous chamber. The maximum average value of DDKL was calculated as the result of the tide-beating status as a result of the output formed in the vitreous chamber status. In Pu-KF-FA-αAVG-MIN, the pulsation far transformation due to pulsation halftone-free-vector-dot beating was shown as 10.90±12.69 units. In pulsation convenient transformation value, Pu-KF-CO-αAVG-MIN was found to be 4.08±3.45. In pulsation flank transformation value, Pu-KF-PU-αAVG-MIN was found to be 0.85±0.76 units. In pulsation vicinage transformation value, Pu-KF-VI-αAVG-MIN was found to be 0.19±0.17 units. By expanding the knowledge level in DDKL, a vitreous chamber status was formed and halftone-free-vector-dot was developed, and the pulsation knowledge figuration ability was evaluated by scattering the vitreous chamber figuration according to the acute-angle. Examining the vitreous chamber using the uneven conveyance technique as a non-invasive technique confirmed that dazzling-divergence figuration, which requires a knowledge level system, appeared due to uneven conveyance. These physical findings enable the functional peculiarity of pulsation knowledge figuration in a vitreous chamber and provide a data base that can be utilized at the vitreous chamber level in a tide knowledge system.