Circular Layer Research Articles

Study of Brunner's Glands Using Histology and Histochemistry in Various Age Groups of Cats, Dogs, and Goats

The purpose of this research is to clarify what Brunner's glands are and how they are distributed in the duodenum of three different dietaryly diverse animals: cats, dogs, and goats. Each species had a total of 20 individuals, which were then divided into four groups of five animals each. The animals were categorized according to their age, which spans from one day to sixty days. After collecting samples from several locations in the duodenum and preserving them with formalin, they were later analyzed histologically. The sections were stained with various dyes, including hematoxylin and eosin, Masson's trichrome, Periodic acid Schiff, Alcian blue PH 2.5, and a mixture of the two stains. All of the species included in the study exhibited an abundance of vacuolated columnar cells in their epithelium-lining villi at the one-day mark. Short and uneven, with intervals in between, were the villi. Except in canines, where the crypts were plainly apparent, the bases of the villi did not have crypts in the lamina propria, but they did contain clusters of immature cells. Also, the muscularis mucosa did not remain constant from one age group to the next. Furthermore, with the exception of goats, a Brunner's gland vanished from the submucosa in as little as one day. It wasn't until the animal was twenty days old, though, that this happened. The muscularis mucosa did not have a completely matured circular layer, but the mucosa contained thin cylindrical villi. During histogenesis, Brunner's glands and the crypts both grew. The villi changed into their mature form in forty days, the crypts were visibly expanding, and the muscularis mucosa formed a circular layer with more Brunner's glands in the submucosa. The mucosa developed mature villi resembling leaves after sixty days. An advanced circular muscular layer covered the muscularis mucosa, and the crypts were more along in their development. Goats have a mix of gland types, in contrast to canines and cats whose duodenal glands are mucous-based. Within the submucosa, the glands were composed of closely packed acini. Positive reactions were detected in dogs and cats only with Periodic acid Schiff, but in goats, they were seen with Alcian blue, Periodic acid Schiff, and a mix of the two. The villi's morphology, crypt count, and gland count were all greatly affected by the age and diet of the subject. Furthermore, this study used a number of morphometrical analyses to show how the lining changed with time.

Morphophysiological and Histopathological Effects of Ammonium Sulfate Fertilizer on Aporrectodea trapezoides (Dugès, 1828) Earthworm.

The present study used the adult earthworm Aporrectodea trapezoides as a bioindicator species to look into the possible dangers of ammonium sulfate (AS) fertilizer. Two complementary toxicity tests were conducted to determine the LC50values, growth rate inhibition, morphological alterations, and histopathological texture of worms. The lethality test included four increasing concentrations of AS fertilizer (ranging from 2500 to 7500 mg/kg of dry soil weight (d.w.)), while sub-lethal concentrations were based on 10%, 30%, 40%, and 50% of the 14-day median lethal concentration (LC50), with a control group included for both tests. The LC(50) values for AS fertilizer were significantly higher at 7 days (4831.13 mg/kg d.w.) than at 14 days (2698.67 mg/kg d.w.) of exposure. Notably, earthworms exhibited significant growth rate inhibition under exposure to various concentrations and time durations (14/28 exposure days). Morphological alterations such as clitellar swelling, bloody lesions, whole body coiling and constriction, body strangulation, and fragmentation were accentuated steadily, with higher concentrations. Histopathological manifestations included severe injuries to the circular and longitudinal muscular layers, vacuolation, muscle layer atrophy, degradation of the chloragogenous tissue in the intestine, collapsed digestive epithelium of the pharynx with weak reserve inclusion, and fibrosis of blood vessels. These effects were primarily influenced by increasing concentrations of fertilizer and time exposure. The study highlights the strong relationship between concentration and exposure time responses and underscores the potential of A. trapezoides earthworms as valuable biological control agents against acidic ammonium sulfate fertilizer. Importantly, this research contributes to the use of such biomarkers in evaluating soil toxicity and the biological control of environmental risk assessment associated with chemical fertilizers.

Multi-mode non-diffraction vortex beams enabled by polarization-frequency multiplexing transmissive terahertz metasurfaces

In terahertz (THz) wireless communication systems, non-diffraction vortex beams carrying an orbital angular momentum (OAM) have attracted extensive attention due to their ability to transmit information over long distances with high capacity. However, existing metasurfaces can only generate a single OAM mode non-diffracting vortex beam at reflection space for circular polarization (CP) incidence, limiting practical applications. To address this issue, we propose and design a polarization-frequency multiplexing transmissive THz metasurface to realize multi-mode non-diffracting vortex beams at linear polarization (LP) incidence. The meta-atom of this metasurface is composed of three anisotropic rectangular metallic structures embedded in vanadium dioxide (VO2) square rings, two circular aperture metallic grid layers, and four dielectric layers. By reasonably designing the size of the metal patch and the state of VO2, the designed metasurface can achieve polarization multiplexing and frequency multiplexing for LP incidence. Based on the phase response of the proposed meta-atoms, the transmissive metasurface can implement not only multi-mode non-diffraction vortex beams but also their space separation at two frequency ranges of 0.80–0.90 THz and 1.50–1.80 THz by changing the state of VO2. Therefore, the proposed multiple multiplexing metasurfaces can effectively shape the wavefront of non-diffraction vortex beams, which have broad application prospects in 6G THz communication.

Successful modified CLEAN-NET with semicircular seromuscular layer incision for a gastric GIST near the cardia: a case report and video demonstration

BackgroundThe combination of laparoscopic and endoscopic approaches to neoplasia with a non-exposure technique (CLEAN-NET) is a laparoscopic and endoscopic cooperative surgery (LECS). It combines laparoscopic gastric resection and endoscopic techniques for local resection of gastric tumors, such as gastrointestinal stromal tumors (GIST), with minimal surgical margins. A conventional CLEAN-NET surgical procedure is complex, requiring careful techniques to preserve the cardia, particularly in case of nearby lesions. We describe the case of a patient who underwent a modified CLEAN-NET approach with a semi-circular seromuscular layer incision surrounding the base of the tumor, different from a circular shape seromuscular layer in the conventional CLEAN-NET: around the tumor to preserve mucosal continuity, which acts as a barrier to avoid intraoperative tumor dissemination.Case presentationA 43-year-old woman was referred to our hospital because of a gastric submucosal tumor near the cardia, detected on medical examination. The patient was diagnosed with gastric GIST based on the results of endoscopic ultrasound-guided fine-needle aspiration. Modified CLEAN-NET was performed with a semicircular incision of the seromuscular layer on the opposite side of the cardia, making the surgical procedure simple and minimizing partial resection of the gastric wall, including the tumor, while preserving the cardia. The operative time was 147 min, preoperative blood loss volume was 3 mL, and postoperative hospital stay was 9 days. The resected specimen revealed a minimal resection of the gastric wall, including the tumor. The cardia and gastric nerves were preserved, and the postoperative food intake was good.ConclusionsThe modified CLEAN-NET with semicircular seromuscular layer dissection is a simple and reliable surgical procedure for GIST near the cardia.

Polarization-sensitive optical coherence tomography and scleral collagen fiber orientation in osteogenesis imperfecta

Osteogenesis imperfecta (OI), a rare genetic connective tissue disorder, primarily arises from pathogenic variants affecting the production or structure of collagen type I. In addition to skeletal fragility, individuals with OI may face an increased risk of developing ophthalmic diseases. This association is believed to stem from the widespread presence of collagen type I throughout various parts of the eye. However, the precise consequences of abnormal collagen type I on different ocular tissues remain unknown. Of particular significance is the sclera, where collagen type I is abundant and crucial for maintaining the structural integrity of the eye. Recent research on healthy individuals has uncovered a unique organizational pattern of collagen fibers within the sclera, characterized by fiber arrangement in both circular and radial layers around the optic nerve head. While the precise function of this organizational pattern remains unclear, it is hypothesized to play a role in providing mechanical support to the optic nerve. The objective of this study is to investigate the impact of abnormal collagen type I on the sclera by assessing the fiber organization near the optic nerve head in individuals with OI and comparing them to healthy individuals. Collagen fiber orientation of the sclera was measured using polarization-sensitive optical coherence tomography (PS-OCT), an extension of the conventional OCT that is sensitive to materials that exhibit birefringence (axial changes in light refraction). Birefringence was quantified and used as imaging contrast to extract collagen fiber orientation as well as the thickness of the radially oriented scleral layer. Three individuals with OI, exhibiting different degrees of disease severity, were assessed and analyzed, along with seventeen healthy individuals. Mean values obtained from individuals with OI were descriptively compared to those of the healthy participant group. PS-OCT revealed a similar orientation pattern of scleral collagen fibers around the optic nerve head between OI individuals and healthy individuals. However, two OI participants exhibited reduced mean birefringence of the radially oriented scleral layer compared to the healthy participant group (OI participant 1 oculus dexter et sinister (ODS): 0.34°/μm, OI participant 2: ODS 0.26°/μm, OI participant 3: OD: 0.29°/μm, OS: 0.28°/μm, healthy participants: ODS 0.38 ± 0.05°/μm). The radially oriented scleral layer was thinner in all OI participants although within ±2 standard deviations of the mean observed in healthy individuals (OI participant 1 OD: 101 μm, OS 104 μm, OI participant 2: OD 97 μm, OS 98 μm, OI participant 3: OD: 94 μm, OS 120 μm, healthy participants: OD 122.8 ± 13.6 μm, OS 120.8 ± 15.1 μm). These findings imply abnormalities in collagen organization or composition, underscoring the necessity for additional research to comprehend the ocular phenotype in OI.

Layer-specific proteomic profiling of human normal heart

BackgroundThe organized functioning of the anisotropic myocardial layers—including the inner longitudinal, middle circular, and outer longitudinal layers—is essential for stable systemic circulation. However, the proteomic profile of each myocardial layer has not been studied yet. Here, we aimed to elucidate the layer-specific proteomic profile of human cardiac tissue using microscopic sampling. MethodsNormal hearts were obtained from five autopsy cases, and cardiomyocytes were microdissected separately from the three myocardial layers of the left ventricle. Histological analysis and shotgun proteomic profiling were performed, followed by immunohistochemical analysis. ResultsHistologically, no significant changes were observed among the three layers regarding cardiomyocyte diameter and myocardial fibrosis. Totally 1220 proteins—comprising 9404 peptides—were identified from 15 samples, of which the expression levels of 92 proteins were significantly altered among the layers. Gene ontology enrichment analysis revealed that the proteins specifically elevated in the inner and outer layers mostly belonged to the actin filament-binding protein group. In particular, MYH1 was highly expressed in cardiomyocytes in the outer layer, and CTNNA3 was highly expressed at the intercalated disc in the inner layer. ConclusionsThis is the first report on layer-specific proteomic profiling of human normal hearts. Anisotropic profiles of actin filament-binding proteins in myocardial layers may contribute to the anisotropic contractile and conductive abilities of the heart. Knowledge of the layer-specific proteome profiles of a human heart in the normal state can aid in further research on cardiac pathology, such as the prognosis and treatment of focal myocardial infarction.

Comparative analysis of molecular dynamics and method of moments in two-dimensional concentric circular layers

In this manuscript, we undertake an examination of a classical plasma deployed on two finite co-planar surfaces: a circular region Ωin into an annular region Ωout with a gap in between. It is studied both from the point of view of statistical mechanics and the electrostatics of continua media. We employ a dual perspective: the first one is by using molecular dynamics (MD) simulations to find the system’s positional correlation functions and velocity distributions. That by modeling the system as a classical two-dimensional Coulomb plasma of point-like charged particles q 1 and q 2 on the layers Ωin and Ωout respectively with no background density. The second one corresponds to a finite Surface Electrode (SE) composed of planar metallic layers displayed on the regions Ωin , Ωout at constant voltages Vin , Vout considering axial symmetry. The surface charge density is calculated by the Method of Moments (MoM) under the electrostatic approximation. Point-like and differential charges elements interact via a 1/r —electric potential in both cases. The thermodynamic averages of the number density, and electric potential due to the plasma depend on the coupling and the charge ratio ξ=q1/q2 once the geometry of the layers is fixed. On the other hand, the fields due to the SE depend on the layer’s geometry and their voltage. In the document, is defined a protocol to properly compare the systems. We show that there are values of the coupling parameter, where the thermodynamic averages computed via MD agree with the results of MoM for attractive ξ=−1 and repulsive layers ξ = 1.

Deep learning for predicting circular retinal nerve fiber layer thickness from fundus photographs and diagnosing glaucoma

PurposeThis study aimed to propose a new deep learning (DL) approach to automatically predict the retinal nerve fiber layer thickness (RNFLT) around optic disc regions in fundus photography trained by optical coherence tomography (OCT) and diagnose glaucoma based on the predicted comprehensive information about RNFLT. MethodsA total of 1403 pairs of fundus photographs and OCT RNFLT scans from 1403 eyes of 1196 participants were included. A residual deep neural network was trained to predict the RNFLT for each local image in a fundus photograph, and then a RNFLT report was generated based on the local images. Two indicators were designed based on the generated report. The support vector machines (SVM) algorithm was used to diagnose glaucoma based on the two indicators. ResultsA strong correlation was found between the predicted and actual RNFLT values on local images. On three testing datasets, we found the Pearson r to be 0.893, 0.850, and 0.831, respectively, and the mean absolute error of the prediction to be 14.345, 17.780, and 19.250 μm, respectively. The area under the receiver operating characteristic curves for discriminating glaucomatous from healthy eyes was 0.860 (95 % confidence interval, 0.799–0.921). ConclusionsWe established a novel local image-based DL approach to provide comprehensive quantitative information on RNFLT in fundus photographs, which was used to diagnose glaucoma. In addition, training a deep neural network based on local images to predict objective detail information in fundus photographs provided a new paradigm for the diagnosis of ophthalmic diseases.

Insights into the role of PGF2α in canine periparturient myometrium.

Parturition in dogs is subjected to complex hormonal regulation, with the involvement of prostaglandin F2α (PGF2α) still not fully understood. To investigate uterine inertia (UI), the most prevalent maternal reason for dystocia in the bitch, a better understanding of undisturbed uterine, especially myometrial function, is crucial. Our aim was to gain deeper insights into the role of PGF2α in the canine parturient myometrium. Uterine biopsies were obtained during medically indicated cesarean sections. To test for stimulatory effects of PGF2α in vitro, circular and longitudinal myometrial layer tissue strips were challenged with 50pM, 0.5µM, and 50µM PGF2α. Prostaglandin-endoperoxide synthase 2 (PTGS2) and PGF2α-receptor (PTGFR) mRNA expressions were compared between primary UI (PUI) and obstructive dystocia (OD) samples in isolated parturient myometrium. PTGFR protein expression was assessed in full thickness uterine samples. PGF2α concentrations were analyzed in canine interplacental tissue around term. In the organ bath, the contractile response to PGF2α was limited to the circular layer at the highest dosage. Correspondingly, PTGFR immunohistochemical staining was significantly stronger in the circular layer (p ≤ 0.01). PTGS2 gene expression did not differ between PUI and OD, whereas PTGFR gene expression could not be quantified. Local uterine PGF2α concentrations correlated negatively with serum P4 levels and were the highest during prepartum luteolysis while being significantly lower in PUI. Conclusively, despite the significant increase in local PGF2α concentrations at birth, confirming the interplacental tissue as a production site, our results suggest that PGF2α might affect uterine contractility during labor, mainly indirectly.

Influence of autonomous regulation type on the connective tissue content in the muscular membrane of the chickens’ small intestine

The relationship between the small intestine's muscular membrane structure of chickens and the autonomous regulation type was investigated. Based on electrocardiographic and variational pulsometric studies, adult laying hens of the Isa-Brown breed were divided into two groups: sympathotonic (ST) and sympatho-normotonic (ST-NT). The volume of individual layers and the entire muscle membrane, as well as the volume of connective tissue fibers located in it, was determined in the small intestine of the hens. In general, the relationship between morphological parameters and the autonomous regulation type is similar throughout the whole intestine, but it has certain peculiarities in different intestine parts. An increase in the volume of both the circumferential and longitudinal layers of the intestinal muscular membrane accompanies an increase in sympathetic tonus. This pattern is typical for both groups. The ratio between these layers varies along the intestinal wall: the share of the longitudinal layer increases while that of the circumferential layer decreases. Accordingly, the average volume of the entire small intestine's muscular membrane in ST and ST-NT chickens differs by 590.6 mm3, and the total volume by 1771.7 mm3. Increased sympathetic tonus also contributes to a more significant number of collagen fibers in the circular layer of the muscle membrane. In the average values of the small intestine, its superiority over the longitudinal layer is 5.6 % in ST chickens and 3.4 % in ST-NT chickens. Regarding absolute values of elastic fiber volume, no group of birds has a complete advantage along the small intestine. However, the average values of this section are still dominated by ST chickens, which outnumber the other group by 15.3 mm3 in the circumferential layer and by 11.7 mm3 in the longitudinal layer of the muscle membrane. Birds with persistent sympathotonia also have more collagen fibers per 1 mm3 muscle membrane. Regarding average small intestine parameters, ST-NT chickens demonstrate a lower value (0.005 mm3). However, regarding the number of elastic fibers alone, ST-NT chickens already occupy a dominant position, and the difference between the two groups decreases to 0.004 mm3. The established muscle membrane's morphological features indicate its plasticity in adapting the intestinal wall to the autonomous regulation peculiarities.

The developmental architecture of the crop of quail embryos (Coturnix coturnix): Morphometric, light and scanning electron microscopy.

The present study focused on the histogenesis of the crop in quail embryos. The developmental sequence was recorded from day 10 of incubation to the hatching day (17th day) by gross anatomy, morphometric analysis and by using light and scanning electron microscope (SEM). Grossly, the crop was distinguished as an enlarged pouch of the cervical oesophagus on the 12th day of incubation. Histologically, the development of the crop began on the 10th day of incubation. The muscularis mucosae was observed as a small, thin layer of myoblast cells on the 10th day, then it appeared as longitudinally arranged smooth muscle fibres with progression of the developmental age till hatching day. The submucosal layer was also observed on the 10th day, while the lamina propria, muscular layer (inner circular and outer longitudinal layers of smooth muscle fibre) and glandular primordia were detected on the 12th day. The epithelial cells of crop revealed affinity for PAS stain and AB-PAS on the 10th day of incubation till the 17th day, while the affinity for AB stain appeared on the 14th day of incubation. Additionally, the glandular cells showed the positive for AB, PAS and AB-PAS on the 12th day of incubation. SEM sections showed variable sizes of the openings of the oesophageal glands. In conclusion, the present study provided a detailed overview of the histogenesis of the crop in quail embryos.

Bi-directional extraordinary absorption, transmission multifunctional metamaterial based on circular metal-VO2 composites

Multifunctional metamaterials play a crucial role in advancing terahertz technology. In this paper, a multifunctional metamaterial composed solely of circular metal-vanadium dioxide (VO2) is designed and numerically demonstrated. The device comprises a top layer of circular VO2 ring, two polyimide dielectric layers, with two metal layers etched and filled with VO2, and a bottom embedded silver (Ag) circular ring dielectric layer. The metamaterial serves as a broadband and narrowband absorptive or transmissive substrate, utilizing the phase transition of VO2 between its metal and dielectric states. When VO2 is in its metal phase, the top circular VO2 ring periodic array functions as a broadband absorber, exhibiting a relative absorption bandwidth of approximately 93%. Meanwhile, the bottom circular Ag ring periodic array functions as a narrowband absorber and exhibits high sensitivity. In contrast, when VO2 is in the insulating phase, the metamaterial acts as a broadband transmittance substrate, exhibiting a maximum transmittance greater than 85%. This proposed metamaterial holds tremendous potential for utilization across various applications within the terahertz band, spanning from terahertz stealth radar to tunable biosensors and photodetectors.

Impact of surface-reflected seismic waves on the seismic isolation performance of circular tunnel isolation layers

Impact of surface-reflected seismic waves on the seismic isolation performance of circular tunnel isolation layers

The Design of a Photonic Crystal Fiber for Hydrogen Cyanide Gas Detection

Hydrogen cyanide gas is a dangerous and fatal gas that is one of the causes of air pollution in the environment. A small percentage of this gas causes poisoning and eventually death. In this paper, a new PCF is designed that offers high sensitivity and low confinement loss in the absorption wavelength of hydrogen cyanide gas. The proposed structure consists of circular layers that are located around the core, which is also composed of circular microstructures. The finite element method (FEM) is used to simulate the results. According to the results, the PCF gives a high relative sensitivity of 65.13% and a low confinement loss of 1.5 × 10−3 dB/m at a wavelength of 1.533 µm. The impact of increasing the concentration of hydrogen cyanide gas on the relative sensitivity and confinement loss is investigated. The high sensitivity and low confinement losses of the designed PCF show that this optical structure could be a good candidate for the detection of this gas in industrial and medical environments.

Role of wall roughness on interaction of leakage flow and main flow in a mixed flow pump with tip clearance

Due to machining precision, spraying coating, cavitation, and erosion, walls of pump flow passage components have different roughness, which will affect the flow state near the wall and even the main flow. In the present work, four types of roughness Ra = 0, 0.1, 1, and 10 μm are set to investigate the influence of wall roughness on the interaction of leakage flow and main flow in a mixed flow pump with tip clearance. Results show that the interaction area of leakage flow and main flow can be divided into three regions: (a) tip clearance zone, (b) shear flow zone, and (c) primary vortex zone. In the tip clearance zone, as roughness increases, the velocity magnitude and its derivative at the same wall distance gradually decrease, and the velocity angle at blade tip continuously increases in the inner circular boundary layer. As the roughness increases, the leakage flow gradually decreases, especially in the center chord region. In the shear flow zone, the velocity gradient rapidly decreases along the direction of pressure side to suction side, and finally, it becomes stable. As roughness increases, the velocity gradient intensity decreases, and the main reason is that the differences of velocity magnitude and velocity direction between the inner circular boundary layer and potential region decrease. In the primary vortex zone, the tip leakage vortex core indicated by the Liutex vortex method coincides with the low-pressure core location. As the roughness increases, the tip leakage vortex core becomes closer to the next blade. The pressure coefficient and vorticity of the tip leakage vortex core decrease with increasing of roughness, and the slopes remain constant on different cross sections with value of 5.9 × 103 and 2.4 × 103 m−1, respectively.

Maturation and development of fetal pig intestinal tissue in immunodeficient mice.

This study aimed to compare the degree of maturation and development of fetal pig segmental intestinal tissue with that of spheroids created by in-vitro reaggregation of dissociated fetal intestinal cells after transplantation into immunodeficient mice. Fetal pig small intestines were transplanted as segmental grafts into the omentum and subrenal capsules of immunodeficient mice or enzymatically treated to generate single cells. Spheroids made by in-vitro reaggregation of these cells were transplanted into the subrenal capsules of immunodeficient mice. The segmental grafts and spheroids were harvested four and eight weeks after transplantation, and the structural maturity and in-vivo development of these specimens were histologically evaluated. The spheroids were engrafted and supplied blood vessels from the host mice, but an intestinal layered structure was not clearly observed, and there was almost no change in size. On the other hand, the segmental grafts formed deep crypts in the mucus membrane, the inner circular layer, and outer longitudinal muscles. The crypts of the transplanted grafts harvested at eight weeks were much deeper, and the smooth muscle layer and the enteric nervous system were more mature than those of grafts harvested at the fourth week, although the intestinal peristaltic wave was not observed. Spheroids created from fetal small intestinal cells could not form layered structures or mature sufficiently. Conversely, segmental tissues structurally matured and developed after in-vivo transplantation and are therefore potential grafts for transplantation.

Histology of the internal reproductive organs of the female Arabian oryx (Oryx leucoryx).

The Arabian oryx (Oryx leucoryx) is an antelope that is considered a "vulnerable" species. Lack of knowledge on the structure of its reproductive organs hinders the development of strategies to improve its reproduction. There is only one report on the gross anatomy of the female reproductive organs. With the aim of describing their microanatomy, the ovaries, uterine tubes, uterus, and vagina were collected at necropsy of an adult female oryx. Samples from each organ were analyzed with H&E staining and light microscopy. The squamous to cuboidal germinal epithelium covers the dense tunica albuginea of the ovaries, which encloses the cortex. This merges smoothly with the medulla. The cortex embeds follicles at several stages of development. A rich bed of blood vessels is present in the medulla, especially at the hilum. The uterine tubes are comprised of a serosa, muscularis, and mucosa. The muscularis is constituted of circular smooth muscle covered by a thin layer of longitudinal fibers. The mucosa has longitudinal folds lined by a pseudostratified epithelium with ciliated cells. The uterine horns are constituted of a serosa, muscularis, and mucosa. The muscularis contains an inner circular layer of smooth muscle and an outer longitudinal one. Simple coiled glands are present at the base of the mucosa. From this, highly cellular projections (caruncles) are present, lined by a by a simple columnar epithelium. The cervix contains four dense rings, and its mucosa is lined by longitudinal folds of a simple columnar epithelium, with ciliated cells. Numerous glands are located in the lamina propria and contain simple cuboidal to columnar epithelium. The vaginal wall was made of a serosa, muscularis, and mucosa. The mucosa is made of stratified squamous epithelium non-keratinized. In summary, the histology of the internal reproductive organs of the oryx is like the one of other ruminants.

PATHOMORPHOLOGICAL CHANGES IN THE ORGANS OF CATS CAUSED BY ALIMENTARY LYMPHOMA. CLINICAL CASES

The article presents the results of clinical, ultrasonographic, pathomorphological and immunohistochemical studies of two random cases of alimentary lymphoma in 9- and 12-year-old domestic cats. Clinical signs of the disease were similar and characterized by anorexia, cachexia, vomiting and diarrhea that appeared in different periods of the disease. The mucous membranes of the oral cavity and eyes were icteric, the abdomen was visually enlarged, palpation revealed nodular thickenings in the abdominal cavity. During the ultrasound diagnostics, diffuse multiple non-encapsulated anechoic formations with uneven edges and growth of tumorous tissue into the intestinal wall were visualized. In another cat, there was an encapsulated formation of a hypoechoic fine-grained structure with well-defined vascularization.
 From the moment of the preliminary diagnosis until the very death, the cats were kept in the clinic under supervision. After the death, an autopsy was performed with the selection of material for histopathological examination. Organs’ fragments were fixed in 10% aqueous solution of neutral formalin and Carnois fluid. Histological preparations were made according to the generally accepted methods. Immunophenotyping of lymphoma type was performed using CD3 (T-cell) and CD79a (B-cell) markers. During the pathological autopsy, an increase in mesenteric lymph nodes, their compaction, and focal thickening of the small intestine wall were revealed. The histological examination showed that jejunum was the most infiltrated intestinal segment, in which massive, diffusely placed monomorphic lymphoblastic cells were localized in the mucous membrane lamina, submucosa and muscle layers. The submucosal layer vessels were sharply dilated, filled with erythrocytes; the muscles of the inner circular layer were swollen. The outer (longitudinal) muscle layer of the jejunum thickened significantly and was intensively infiltrated by lymphoblastic cells.
 Typical diffuse lymphoblastic proliferations were also detected in the cortical substance of lymph nodes and lymphoid nodules. Stasis and significant expansion of the marginal sinus vessels were visualized too. In addition, moderate mononuclear lymphoblastic infiltrates were detected in the pancreas parenchyma with their perivascular location. Immunohistochemically, a significant positive expression of CD3 cells was detected both in the jejunum mucous membrane and the marginal sinus area, around the germinal centers of the lymph nodes, which indicated a T-cell type of lymphoma.

Ultrasonography along with Clinical Finding is enough for the Diagnosis of Infantile Hypertrophic Pyloric Stenosis

Introduction: Infantile hypertrophic pyloric stenosis (IHPS) is characterized by hypertrophy of the pyloric muscular propria, predominantly involving the circular layer and subsequent narrowing of the pyloric outlet. Aim of the Study: To see the ultrasonographic diagnosis of infantile hypertrophic pyloric stenosis. Material & Methods: This retrospective study was conducted in Dhaka Shishu (Children) Hospital, Dhaka, Bangladesh during the period conducted from February 1997 to October 1998 in a period of 21 months. The data were collected for all 31 patients. Following, collection of all the required data, these were checked and tabulated into the computer using the SPSS/PC software 23. Results: During the study period February 1997 to October 1998 total surgical admission was 4006 and infantile hypertrophic pyloric stenosis patients (IHPS) were 31. So IHPS represented .77% of the total surgical admission during the study period in DSH. Correlation of clinical and ultra-sonogram findings with operative finding, clinical findings true +ve 20(64.52%), true –ve 2(6.45%), false +ve 1(3.23), false –ve 8(25.81) and ultra-sonogram observation true +ve 27(87.10%), true –ve 2(6.45%), false +ve 2(6.45%), false –ve 2(6.45%) and then finally operation needed IHPS ware 29(93.35%) also others than IHPS 2(6.45%). Correlation of pattern of sensitivity and specificity in clinical findings 93.1% and 50%, ultra-sonogram findings 71.43% and 66.67%. Conclusion: In conclusion, we would like to advise that infantile hypertrophic pyloric stenosis is the most common cause of non-bilious projectile vomiting in an infant. The diagnosis being mode primarily by non-bilious projectile vomiting in a neonate, which usually starts 2-4 weeks after delivery and ultrasonography can confirm the diagnosis.

Loss of α-smooth muscle actin immunostaining is not a useful marker for functional impairment: a comparison from patients with and without small bowel motility disorder

Abstract Introduction/Objective Prior studies have reported the loss α-smooth muscle actin (α-SMA) immunostaining in the inner circular layer of the muscularis propria in small bowel motility disorder cases, but this remains controversial with conflicting data. In this study we aimed to characterize the immunoreactivity of α-SMA expression in the muscularis propria of the small intestine, specifically jejunum, in patients with and without small bowel motility disorder. Methods/Case Report A total of 28 samples of transmural proximal jejunum biopsies from adult patients with small bowel motility disorder by manometric and/or radiologic testing and 47 control tissues were evaluated. The controls were full thickness, longitudinal tissue sections from segmental resections performed due to trauma from gunshot wound (n= 30), multi-visceral transplant for neoplasms (n= 4), liver failure with concurrent portal vein thrombosis (n= 9), and isolated intestinal transplant for Crohn disease (n= 4). Immunostaining for α-SMA was performed with appropriate controls to confirm the presence of staining in the circular and longitudinal muscle layers of the muscularis propria in each sample and recorded as retained or diminished. Results (if a Case Study enter NA) In the small bowel motility disorder and control cases, 43% (12/28) and 62% (29/47) of the cases showed no or minimal α-SMA expression in the inner circular layer with peripheral accentuation (p = 0.113), respectively. The remaining small bowel motility disorder cases (16/28, 57%) and the control group (18/47, 38%) showed retained expression of α-SMA in both layers of the muscularis propria. Conclusion Loss or diminished α-SMA expression in the inner circular layer of muscularis propria is present in similar frequency in cases with and without small bowel motility disorder and does not correlate with impairment of function.