Aquaculture systems are an important economic activity, and rustic aquaculture represents a sustainable production alternative in the world while also, providing suitable conditions for the establishment of epibiotic ciliates on crayfish, however its impact on aquaculture production has been little studied. The objective of this work was to identify the epibiotic ciliates on the surface of the crayfish Procambarus (Austrocambarus) sp. and analyze if there is any correlation between seasonality and environmental conditions in an artificial pond in Chiapas, Mexico, regarding the establishment of epibiotic relationships in a rustic aquaculture system. The crayfish were collected during dry and rainy seasons to analyze their ciliate epibionts. Some physical and chemical parameters of the water were measured and correlation matrix analyses were applied to detect the possible correlations between environmental parameters and the presence of ciliates. We identified thirteen species belonging to the subclasses Peritrichia and Suctoria. Significant differences were found for some environmental parameters, comparing correlation coefficients for three species recorded in both seasons resulted in statistically significant differences between seasons. This data could help to elucidate the relation between the epibiotic community and its environment and to understand better the ecological interaction and its implications in aquaculture.