Granulation Research Articles

A Case of MIRAGE Syndrome with SAMD9 Mutation and Refractory Infantile Diarrhea: Endoscopic Biopsy Evaluation via Light and Electron Microscopy.

An infant with intrauterine growth restriction, suspected of having MIRAGE syndrome based on prenatal ultrasound, presented with genital ambiguity, adrenal insufficiency, intractable diarrhea from birth, and a pathogenic SAMD9 mutation (c.1376G>A, p.R459Q). Endoscopic biopsies of the duodenum revealed complex light and electron microscopic abnormalities. Hypoplastic villi without signs of enteritis suggests a disorder of mucosal growth with reduced absorptive surface area contributes to intractable diarrhea. Ultrastructural study showed prominent dilated endoplasmic reticulum, abnormalities of Golgi morphology, specialized granule, and mucin processing. We hypothesize that the SAMD9 mutation alters mucosal growth, and the processing of mucin, Paneth and neurosecretory granules, with premature degradation of specific granules in enterocyte lysosomes. These distinctive morphological findings support the idea that multisystem manifestations of MIRAGE syndrome are due to a primary disorder of microsomal trafficking.

COMBINED THERAPY OF OZONIZED OIL AND PHOTOBIOMODULATION IN THE TREATMENT OF CHRONIC WOUNDS

Hard-to-heal wounds pose significant therapeutic challenges due to pathogenic microorganisms and associated infections, leading to delays in tissue healing. The present study aimed to carry out a clinical trial to identify the microbial agent present in hard-to-heal wounds on the lower limbs, assess the antimicrobial effects of ozonated oil and examine the benefits of photobiomodulation combined with ozonated oil in the treatment of those wounds. Then we examined the antimicrobial kinetics of ozonated oil on microorganisms found in wounds by an in vitro study. For this study, some commercial ozonated sunflower oil was used as well as the LED photobiomodulation therapy, 660 nm, 30 seconds, punctual 2 cm away on the wound bed, 120 mW, 3.6 J per point. The procedure was performed three times a week. The clinical results evidenced a significant healing of injuries treated with ozonated oil and photobiomodulation, evidenced by a reduction in traces of infection, presence of granulation tissue, wound area reduction and, in some cases, full wound closure. Among the eight patients monitored, only one had negative microbiology. Among the microorganisms identified are Klebsiella sp, Staphylococcus epidermidis and Escherichia coli, all proved resistant to the antibiotics tested. In vitro results confirmed the antimicrobial efficacy of ozonated oil, demonstrating its potential as a viable therapeutic agent for chronic wounds in all microorganisms tested when subjected to concentrations deemed low, ranging from 1.6% to 12.5%, demonstrating antibacterial activity. In conclusion, ozonated oil associated with photobiomodulation therapy represents a promising resource for the treatment of hard-to-heal wounds. However, new clinical trials are necessary to establish more improved treatment protocols.

Innovative Approaches in Fistula-In-Ano Management: Evaluating the Role of Fistula Brush - A Case Study

Introduction: Fistula in ano is one of the most common anorectal diseases. The prevalence is greater in men than women, with the rate of 12.3 cases per 100,000 and 5.6 cases per 100,000, respectively. In Ayurvedic Science Ksharasutra ligation is one of the most popular treatment modality for Bhagandara. Ksharasutra is indeed a well-known treatment in Ayurveda, particularly for managing conditions like pilonidal sinus and anal fistulas. However, it does have some limitations. Hence, an innovative technique is adopted by introducing a new Yantra i.e., Fistula brush in the field of Shalya Tantra for minimizing the unit cutting time in fistulous tract. Methodology: The fistulous tract was curetted using the fistula brush in 4-5 strokes followed by primary threading with barbour thread. Follow up thread change was done after curetting the tract using fistula brush until the tract was cut through. (under local anaesthesia). Result: The intervention results indicated a marked improvement in both subjective and objective parameters, demonstrating the intervention's effectiveness in enhancing patient outcomes. Discussion: The treatment of anal fistula through curettage of the tract using a fistula brush has been demonstrated to be a cost-effective and accessible method. This technique facilitates the removal of unhealthy granulation tissue while ensuring adequate drainage with the use of a drainage seton, thereby promoting the formation of healthy granulation tissue. Ultimately, this process encourages fibrosis of the tract and minimizes the risk of sphincter damage. Mechanical debridement plays a pivotal role throughout the stages of wound healing by promoting a clean, healthy wound environment. By alleviating symptoms associated with each stage - such as pain, burning sensation, discharge and local tenderness - it ultimately enhances the healing process and improves patient comfort.

Role of Daruharidra Rasakriya Lepa in the management of Dushta Vrana w.s.r. to Chronic Non-Healing Ulcer : A Clinical Study

Background: Vrana is an interruption of continuity of body tissue or part by physical, microbial, chemical or immunological causes, typically associated with loss of function. Vrana can be correlated with wounds and ulcers. Normally, Vranas are healed by itself if kept clean. Wound factors delay wound healing. The presence of infection, insufficient blood supply, tissue tension, radiation, malnutrition, diabetes, consumption of steroids etc. are the important factors which delay the wound healing process. Dushta Vrana is one of the Vranas which needs time for its healing. Acharya Sushruta mentioned Rasakriya under Shasti Upakramas for management of Vrana. Any drug formulation should possess two qualities for proper wound healing i.e., Vrana Shodhana (Making free from undesirable wound factors) and Vrana Ropana (Closure of the wound). Many formulations are in use for centuries. One of them is Daruharidrarasakriya. The objective of the present study is to evaluate the effect of Daruharidraraskriya on the basis of relief in signs and symptoms of Dushta Vrana. Materials and Methods: In this study, Lepa of Daruharidrarasakriya was applied on affected part of 25 patients selected from Shalya OPD, GAMC&H, Akhnoor, for the management of Vrana. Results: The results observed based on the relief obtained on the subjective and objective parameters taken for consideration for this study viz. Pain, Size of ulcer, Discharge, Base (granulation tissue) and were found significant on all the above parameters. Conclusion: It showed that Daruharidraraskriya significantly helped in reduction of the signs and symptoms of the Dushta Vrana, enhancing wound healing process and reducing the morbidity of the patients.

Melanosis Contributing to Downgrading in Processed Broiler Chickens in Argentina: Preliminary Diagnostic Findings

Melanosis is scarcely reported as a potential cause of carcass downgrading in broilers worldwide. We described the preliminary diagnostic findings of dermal and celomic fat tissue melanosis in a total of four episodes of increased downgrading in 31-to-50-day-old male Cobb500TM broiler chicken carcasses at a processing plant in Argentina from November 2016 to March 2022. Veterinary inspectors evaluated and sampled a total of 10 carcasses. Gross findings included the gray discoloration of subcutaneous tissue in wings and legs, celomic fat tissue and peritoneum. Microscopically, melanocytes infiltrated the connective tissue and adipose cells of subcutaneous tissue in the skin of legs and wings as well as the celomic fat tissue of all 10 birds. The Fontana–Masson stain revealed black melanin granules in subcutaneous tissue and celomic fat tissue, demonstrating its value as a histologic tool to confirm grossly compatible melanosis in all of the evaluated tissues sampled at the broiler processing plant. We propose to add melanosis as one of the less frequently and rare diagnosed cutaneous darkening conditions which potentially contributes to downgrading in chicken processing plants globally.

Evaluating Resonant Acoustic Mixing as a Wet Granulation Process

Evaluating Resonant Acoustic Mixing as a Wet Granulation Process

Injectable Nanocomposite Hydrogel with Synergistic Biofilm Eradication and Enhanced Re-epithelialization for Accelerated Diabetic Wound Healing.

Diabetic wounds remain a critical clinical challenge due to their harsh microenvironment, which impairs cellular function, hinders re-epithelialization and tissue remodeling, and slows healing. Injectable nanocomposite hydrogel dressings offer a promising strategy for diabetic wound repair. In this study, we developed an injectable nanocomposite hydrogel dressing (HDL@W379) using LAP@W379 nanoparticles and an injectable hyaluronic acid-based hydrogel (HA-ADH-ODEX). This dressing provided a sustained, pH-responsive release of W379 antimicrobial peptides, effectively regulating the wound microenvironment to enhance healing. The HDL@W379 hydrogel featured multifunctional properties, including mechanical stability, injectability, self-healing, biocompatibility, and tissue adhesion. In vitro, the HDL@W379 hydrogel achieved synergistic biofilm elimination and subsequent activation of basal cell migration and endothelial cell tube formation. Pathway analysis indicated that the HDL@W379 hydrogel enhances basal cell migration through MEK/ERK pathway activation. In methicillin-resistant Staphylococcus aureus (MRSA)-infected diabetic wounds, the HDL@W379 hydrogel accelerated wound healing by inhibiting bacterial proliferation and promoting re-epithelialization, regenerating the granulation tissue, enhancing collagen deposition, and facilitating angiogenesis. Overall, this strategy of biofilm elimination and basal cell activation to continuously regulate the diabetic wound microenvironment offers an innovative approach to treating chronic wounds.

Comparison between negative pressure wound therapy and platelet-rich plasma therapy in management of diabetic foot

Background: Foot infections and ulceration are the most frequent reason for hospitalization in patients with diabetes mellitus (DM), accounting for up to 25% of all patients with DM admission. In the new advanced therapies for treating diabetic foot ulcers (DFUs), platelet-rich plasma (PRP) and negative pressure wound therapy (NPWT) have shown promising results. Aims and Objectives: The objectives of the study are as follows: To study the role of NPWT and PRP in management diabetic foot wounds and to compare granulation tissue formation, total duration of wound healing, and hospital stay between two therapies in diabetic foot wounds. Materials and Methods: This study was conducted with 100 patients of diabetic foot wounds meeting the inclusion criteria with pre-structured proforma and written informed consent for the therapies. After surgical debridement of the wound, the enrolled patients were randomized into two groups to receive either PRP therapy (Group A) (n=50) or NPWT (Group B) (n=50). Results: PRP and NPWT both were effective in healing process of diabetic foot patients. The mean value of Group A in granulation tissue score were 2.10±0.73 and 1.90±0.60 in Group B showed no significant difference. The duration of hospital stay was lower in Group A as compared to Group B that is 19.04±4.85 days in Group A and 35.86±7.35 days in Group B. The total duration of wound healing in Group A was longer that is 7.22±3.34 weeks in Group A and Group B was 4.0±2.27 weeks. Conclusion: PRP (Group A) and NPWT (Group B) are novel tools in management of DFUs. PRP is more simple, more safe, less costly, less equipment requirement, less complication, shorter time for the method, less painful, less hospital stay, and autologous nature in the preparation and had proved the superiority over NPWT.

Treatment of a staple-line leak after laparoscopic sleeve gastrectomy using an esophagoduodenal Niti-S Mega-stent.

A 56-year-old female with class 3 obesity, was previously submitted to laparoscopic sleeve gastrectomy (LSG) two weeks before current admission. The patient was admitted due to fever, abdominal pain and vomiting. CT revealed a 13.5cm collection between the gastric wall and left hepatic lobe. Piperacillin-Tazobactam was initiated, and percutaneous drainage of the purulent collection was performed. The upper endoscopy showed pus in the gastric lumen and a 10-mm leak at the esophago-gastric junction with contrast extravasation. Argon plasma coagulation (40W, 2L/min) was applied at the orifice margins to promote healing. After 0,038'' guidewire placement through the proximal jejunum, a Niti-S™-MEGA™ (Mega-Stent) 28x230mm (Taewoong Medical™) was deployed under fluoroscopic control. CT at 72h showed absence of leakage. Oral nutrition was started uneventfully. Ambulatory endoscopy was scheduled for eight weeks later to remove the stent, which was easily accomplished with a foreign body forceps. Granulation tissue was observed in the previous leak location and no extraluminal contrast was observed at fluoroscopic evaluation, confirming complete healing and successful endoscopic treatment. Three weeks after stent removal, the patient presented with dysphagia. Upper endoscopy revealed a short and narrowed lumen stenosis at 28cm from the incisives, which was easily manage through TTS balloon dilation. No additional complications were observed.

Treatment of external root resorption with Biodentine in a tooth with a periodontal pocket: a case report with 2-year follow-up

External root resorption is characterized by the loss of mineral tissues in the dental root. This condition is difficult to diagnose, and if not identified early, it can lead to tooth extraction. The treatment consists of surgical access, removal of granulation tissues, and sealing the area with a biological material. Objective: This article reports on the treatment of ERR in the middle third of the root, associated with an 8 mm periodontal pocket using Biodentine, with a two-year follow-up. Case report: A 57-year-old woman attended complaining of discomfort in the palate. After a thorough medical history, clinical, radiographic, and tomographic evaluation, the final diagnosis was External Root Resorption. Concerned about preserving the tooth, it was suggested to fill the resorption with Biodentine and regular follow-ups. The patient agreed, and a tissue flap was raised in the palatal region, followed by sealing of the perforation with Biodentine and suturing. After 7 days, the sutures were removed, and during the six-month and two-year follow-ups, the tooth exhibited no mobility, good periodontal health, and maintained its function. Conclusion: The case demonstrated a reduction in the periodontal pocket (from 8 mm to 4 mm), maintenance of the tooth, and the effectiveness of Biodentine in treating ERR, even when exposed to the oral cavity.

Analysis of the Impact of Extraction Processes on the Quality of Traditional Chinese Medicine Granules.

This review aims to summarize and elucidate the extraction processes of traditional Chinese medicine granules, examining their connection to quality control, with the aim of establishing a foundation for superior extraction methodologies. This article systematically collects and analyzes relevant literature from the past two decades through databases such as Science Direct, PubMed, Web of Science, Google Scholar, and CNKI, focusing on the extraction processes of traditional Chinese medicine granules and the factors affecting extraction efficiency. This article provides a comprehensive account of the extraction and granulation processes of traditional Chinese medicine granules. Beginning with the physicochemical properties and chemical components of herbal slices, it explores ways to improve extraction efficiency while also examining the relationship between component structure and biological activity. Rooted in the fundamental theories of traditional Chinese medicine and harmonized with contemporary extraction technologies, it follows the principle that "quality originates from design," ensuring stringent control over the extraction process. This article analyzes extraction technologies, factors influencing extraction efficiency, and quality control measures, establishing a theoretical basis for achieving high-quality and high-yield extraction processes.

Imaging and clinical significance of tubular cell in lateral facial recess

Objective:To observe the position and morphology of tubular cell in lateral facial recess by CT scanning and surgical finding, and its clinical significance. Methods:Thirty patients（32 ears） with cholesteatoma and/or adhesive otitis media requiring radical mastoidectomy and tympanoplasty were included in this study. To observe the morphology of the tubular cell in the lateral facial recess cavity through temporal bone HRCT and surgery, and to analyze its relationship with the facial nerve. Results:Tubular cell were found in 59.4%（19/32） ears by temporal bone HRCT and through surgeon. The length ranged from 1.5-6.5 mm, with median length of 2.5 mm. All of them opened in the facial recess, ran on the posterior wall of the facial recess, and even reached the bottom of the inferior tympanic cavity or the level of the inferior wall of the external auditory canal（sagittal view and surgical findings）. Probing from the inside out, the tubular cell located between the second genu of the facial nerve and the chorda tympani, which was a part of the lateral wall of the facial nerve（facial crest）. When the tubular cell is occupied by granulation tissue, it is easy to be regarded as the facial nerve. Fully drilling out the tubular cell is helpful to cut down the facial nerve crest. In the study, the follow-up and/or postoperative no facial paralysis happens during the operation and in the follow up. Conclusion:Preoperative temporal bone HRCT can clearly show the tubular cell in the lateral facial recess cavity. Comprehensive and accurate identification of these structures is helpful to fully cut down the facial nerve crest and remove the hidden lesions in the posterior tympanic cavity.

Construction of multifunctional hydrogel containing pH-responsive gold nanozyme for bacteria-infected wound healing

Nanozymes have become promising alternative antibacterial agents for bacteria-infected wounds. In this study, fucoidan-confined gold nanoparticles (Fuc@AuNPs) are developed by in situ reduction, and stabilized by sulfate groups of fucoidan. Fuc@AuNPs exhibit pH-responsive catalytic activity that can mimic oxidase (OXD) under acidic bacterial infection conditions and mimic superoxide dismutase (SOD) under normal physiological conditions. The OXD-like catalytic activity of Fuc@AuNPs generates active singlet oxygen (1O2), exhibiting effective antibacterial properties against both Gram-negative E. coli and Gram-positive S. aureus. Fuc@AuNPs and aldehyde grafted saponin incorporate with chitosan to form a hybrid hydrogel. This hydrogel exhibits superior mechanical, adhesive, and self-healing properties due to electrostatic complex coacervation networks and dynamic covalent Schiff base reactions. Animal experiments show that the hydrogel aids S. aureus-infected skin wound healing by reducing bacterial infection and promoting granulation tissue formation without causing excessive ROS-induced inflammation. This study presents the design of multifunctional nanozymes and bioactive hydrogels as a promising wound healing dressing for biomedical applications.

Mass granulation of Al-promoted CaO-based sorbent via moulding-crushing methods for cyclic CO2 capture

Calcium looping (CaL) process, as an effective way to achieve CO2 mitigation from high-temperature flue gas streams, is one of the most promising alternatives to amine scrubbing (a well-established technology for industrial post-combustion CO2 capture). CaO sorbent is considered to be an ideal CO2 adsorption material. Moreover, the development of granulation/pelletization techniques along with the mass preparation of the CaO-based sorbent is imperative for realistic large-scale applications. This work proposes two practicable moulding-crushing techniques for the scale-up granulation of CaO-based sorbents, in which the kilogram-scale produced Al-promoted CaO-based sorbent powders were first moulded and subsequently crushed into the granules of target sizes. Three types of organic acids–acetic acid, citric acid and malonic acid were employed as peptizing agents to optimize the granulation process. As a result, the anti-attrition properties and compressive strength of the synthetic sorbents were elevated owing to the introduction of an appropriate amount of acetic or malonic acid, for it expedited the disintegration of the pseudo-boehmite (served as binder agent) particles into sol particles, which allowed for tighter bonding of sorbent particles. In addition, corncob powder acted as a pore-forming agent, enhancing the porous structure of the sorbent particles due to the gases released from the thermal decomposition of organic groups during calcination. Nevertheless, the results revealed that the porous and loose structure adversely affected the mechanical strength of the granules.

Diagnosis and management of external auditory canal cholesteatoma (EAC)

Introduction: Cholesteatoma is a lesion formed from keratinized stratified squamous epithelium that occurs in the temporal bone and is often found in the middle ear. Cholesteatoma of the ear canal, commonly referred to as External Auditory Canal Cholesteatoma (EAC), involves the invasion of squamous epithelial tissue into the bone of the external auditory canal, which is locally aggressive and erodes the bone. Other terms introduced by experts include keratoma, squamous epitheliosis, cholesteatosis, epidermoid cholesteatoma, epidermoid cyst, and epidermosis. Management of external auditory canal cholesteatoma can be either conservative or surgical. Conservative treatment is indicated when the cholesteatoma and bone erosion are limited to the external auditory canal. Surgical intervention is required when there is extension to the middle ear or mastoid, the presence of complications or potential complications, persistent otorrhea not responsive to conservative therapy, or significant hearing loss. Case Report: We report a case of a 21-year-old woman with External Auditory Canal Cholesteatoma (EAC). Conclusion: The definitive diagnosis of cholesteatoma is made through histopathological examination revealing benign keratinized squamous cells. Extirpationf of granulation tissue and cholesteatoma via a transcanal approach was performed, involving the removal of debris and keratin through surgery. The pathological tissue was scraped until normal skin margins were achieved. Eroded bone was cleaned, and granulation tissue was removed until healthy tissue was identified to facilitate healing.

Efficacy of dermapen combined with topical insulin versus dermapen combined with topical hyaluronic acid in treatment of stretch marks: comparative study.

Striae distensae (SD) are visible linear scars developing in areas of dermal damage. Insulin is a peptide hormone that can potentially help restore the integrity of damaged skin and promote the reformation of granulation tissue. Hyaluronic Acid is commonly used in aesthetic medicine. To evaluate the clinical response and efficacy of microneedling and topical insulin against microneedling and topical hyaluronic acid in treating stretch marks. The study was conducted on 30 patients suffering from striae distensae in the abdomen. The patients were treated by microneedling followed by hyaluronic acid on the right side. The second group was treated by microneedling followed by topical insulin on the left side of the abdomen. At the end of the study the improvement with hyaluronic acid was 23.3% of patients with excellent improvement and 30.0% of patients with marked improvement. The improvement with topical insulin was 30.0% of patients with excellent improvement and 20.0% of patients with marked improvement. Microneedling followed by hyaluronic acid or topical insulin can be an effective and safe therapeutic option for the treatment of Stretch marks with no significant side effects.

“Chasing Rainbows” Beyond Kaposi Sarcoma’s Dermoscopy: A Mini-Review

The dermoscopic rainbow pattern (RP), also known as polychromatic pattern, is characterized by a multicolored appearance, resulting from the dispersion of polarized light as it penetrates various tissue components. Its separation into different wavelengths occurs according to the physics principles of scattering, absorption, and interference of light, creating the optical effect of RP. Even though the RP is regarded as a highly specific dermoscopic indicator of Kaposi’s sarcoma, in the medical literature, it has also been documented as an atypical dermoscopic finding of other non-Kaposi skin entities. We aim to present two distinct cases—a pigmented basal cell carcinoma (pBCC) and an aneurysmatic dermatofibroma—that exhibited RP in dermoscopy and to conduct a thorough review of skin conditions that display RP, revealing any predisposing factors that could increase the likelihood of its occurrence in certain lesions. We identified 33 case reports and large-scale studies with diverse entities characterized by the presence of RP, including skin cancers (Merkel cell carcinoma, BCC, melanoma, etc.), adnexal tumors, special types of nevi (blue, deep penetrating), vascular lesions (acroangiodermatitis, strawberry angioma, angiokeratoma, aneurismatic dermatofibromas, etc.), granulation tissue, hypertrophic scars and fibrous lesions, skin infections (sporotrichosis and cutaneous leishmaniasis), and inflammatory dermatoses (lichen simplex and stasis dermatitis). According to our results, the majority of the lesions exhibiting the RP were located on the extremities. Identified precipitating factors included the nodular shape, lesion composition and vascularization, skin pigmentation, and lesions’ depth and thickness. These parameters lead to increased scattering and interference of light, producing a spectrum of colors that resemble a rainbow.

Combined Effects of Bone Marrow Cells and Pulsed Microwaves on Thermally Damaged Skin of Laboratory Rats.

We studied the effect of bone marrow cells (BMC) and nanosecond repetitively pulsed microwaves (RPMs, 10 GHz, pulse duration 100 nsec, pulse repetition rate 8 Hz, peak power flux density (pPFD) 140 W/cm2) on the regeneration of thermally damaged skin in rats. The combined use of BMC and RPMs accelerates separation of an eschar with its complete rejection on day 14 of the experiment. Histological analysis demonstrated a statistically significant increase in the relative area of the granulation tissue and thickness of newly formed epidermis in the group with combined exposure. Combined therapy ensured completion of wound epithelialization in 100% of rats by day 30 of the study.

Preparation and tribological properties study of a novel self-lubricating Alumina-based composite coating

A self-lubricating composite coating Al2O3/C was designed and sprayed via atmospheric plasma spraying (APS), in order to endow the alumina coating with certain lubricating properties under room temperature conditions. The raw feeding powders of boehmite-alumina-graphite (AlO(OH)/Al2O3/C) with particle sizes of 30–50 µm were prepared through hydroxylation treatment, physical stirring, and spray granulation process. The tribological properties of the Al2O3/C composite coating were systematically studied using a CSM friction tester. The prepared Al2O3/C composite coating exhibited fewer defects, lower coefficient of friction (COF), and slighter adhesion wear degree compared to single-component Al2O3 coating. The graphite lubrication film was discovered on both the worn surface of the composite coatings and the friction pair after friction. The existence of the graphite lubrication film decreased the COF, and the reduction extent depended on the load, with a maximum reduction of 55 %. The composite coatings presented the best tribological properties when sliding against Si3N4 balls at a higher load (5 N) because of the diverse wear mechanisms and the influence of graphite lubrication film, resulting in a reduction in the COF and wear rate by approximately 48 % and 11 times respectively. In addition, part of γ-Al2O3 in the Al2O3/C composite coating transformed into α-Al2O3 after the friction test. But the Al2O3 coating remained phase stable, with no phase change occurring before and after the friction. The transformation from the γ-Al2O3 to the α-Al2O3 phase was positive as for the tribological performance. It could reduce the COF as well as improving the thermal conductivity and mechanical properties of the coating.

Microfluidic-engineered Chinese herbal nanocomposite hydrogel microspheres for diabetic wound tissue regeneration

Microfluidic-engineered hydrogel microspheres have emerged as a promising avenue for advancements in tissue engineering and regenerative medicine, particularly through the precise manipulation of fluids to achieve personalized composite biomaterials. In this study, we employed microfluidic technology to fabricate hydrogel microspheres (HMs) using Chinese herbal Bletilla striata polysaccharide (BSP) as the primary material. Concurrently, the natural active ingredient 20(S)-protopanaxadiol (PPD) was encapsulated within the HMs in the form of liposomes (PPD-Lipo), resulting in the formation of nanocomposite hydrogel microspheres (PPD-Lipo@HMs) intended for diabetic wound tissue repair. PPD-Lipo@HMs are characterized by the expansive specific surface area, adjustable mechanical properties, and exceptional biocompatibility. PPD-Lipo@HMs can stimulate the production of vascular endothelial factors, which in turn enhances the migration of endothelial cells, the creation of tubes, angiogenesis, and tissue repair. Moreover, the PPD-Lipo@HMs accumulation produces a microsphere scaffold that effectively covers damaged tissues, promoting the attachment, spread, and multiplication of fibroblast and endothelial cells. The polysaccharide material BSP within PPD-Lipo@HMs can modulate the immune microenvironment of the damaged tissue, reducing inflammation, encouraging re-epithelialization and granulation tissue formation, accelerating angiogenesis and collagen deposition, ultimately leading to tissue repair. The findings highlight the superior therapeutic efficacy of the microfluidic-engineered PPD-Lipo@HMs in addressing the complex challenges of diabetic wound tissue repair, thereby affirming the significant potential of microfluidic engineering technology in tissue repair applications.