This study aims at the assessment of the environmental status and microbial diversity prevailing in the overburden soil of South Kaliapani chromite mine area, Sukinda, India. It is found that the overburden soils/dumps are dry and semi-hard rocky material, and contain low nutrients (N, P, K and organic carbon). Major metal components present in the dump soils are (wt%): 2-24 Cr, 33-58 Fe, 0.4-0.5 Mn, 0.5-1.2 Ni, and 0.02-0.04 Co. The overburden soils have low microbial population (bacteria 7 − 29 × 105 colony forming units (cfu) g−1 and fungi 3 − 9 × 105 cfu g−1) due to the presence of Cr(VI), a toxic pollutant. The water samples contained Cr(VI) (0.97 mg L−1) and Co (0.345 mg L−1), which were higher than the permissible limits. Oxidation of Cr(III) of the chromite mineral present in the chromite ore overburden (COB) by manganese oxides is indicated by SEM-EDS studies which causes Cr(VI) pollution and affects microbial diversity.