Chromatogram Profiles Research Articles

MEMBANGUN DESA MANDIRI ENERGI MELALUI PENGOLAHAN SAMPAH PLASTIK MENJADI BBM ALTERNATIF DI DESA HANURA KECAMATAN TELUK PANDAN PESAWARAN

The market in Hanura Village faces serious problems related to rubbish, especially because it is located close to the main beach tourist route which is busy during holidays. Increased activity in markets produces more waste, which is usually simply transferred to landfill, but this method is less effective. Plastic waste is the second biggest problem after organic waste and is difficult to decompose naturally, so other solutions are needed to reduce waste accumulation. To tackle this issue, the Unila community service team proposes a more efficient plastic waste management system. Drawing from Bahri et al., (2019), their research demonstrates that plastic waste can be converted into alternative fuel through a pyrolysis process, utilizing a device known as a pyrolizers. With an abundant supply of plastic waste and Hanura Village’s strategic location, the development and application of this process are being implemented through a community service program based on research dissemination. The result of this activity is a plastic waste processing tool that converts waste into alternative fuel in the form of a pyrolyzer with three condensers. The fuel produced from the pyrolysis of plastic waste is characterized by its chromatogram profile using Gas Chromatography Mass Spectrometry (GCMS), and its calorific value is measured using a bomb calorimeter, while the octane number is determined using an Octane Number Tester. The results of the community service activity in Hanura Village cognitively show that the residents of Hanura Village were highly enthusiastic about receiving the training, especially related to the processing of plastic waste into fuel, with an overall achievement of specific instructional objectives reaching 53%.

Fingerprint Metabolite of Miana (Coleus sp.) Leaf Infusion and Juice: Authentication Based on FTIR Spectroscopy and Multivariate Analysis

Miana (Coleus sp.) is a plant belonging to the Lamiaceae family and has been widely used in traditional medicine. Despite the potential of the plant, the quality and quantity of chemical components that can be extracted are dependent on various factors, such as varieties and extraction techniques. Therefore, this study aimed to discriminate miana based on varieties and extraction techniques as well as identify its marker functional groups. The 4 different leaf varieties of the plant, including purple, green, batik, and color combination were extracted using the infusion method, both with and without the addition of water. FTIR (Fourier transform infrared) fingerprint metabolite of each extract was then analyzed with IRPal 2.0 software for functional group interpretation and multivariate data analysis (PCA and PLS-DA) using MetaboAnalyst 5.0. The results showed that the chromatogram profile of all varieties and extraction techniques had similar patterns. The PCA (principal component analysis) score plot yielded a total PC value of 98.5%, which could effectively discriminate the samples. In addition, the O–H group’s VIP score value (greater than 1) was a significant functional group that characterized the samples. Based on these results, the combination of FTIR spectroscopy and multivariate analysis could be used to discriminate miana based on varieties and extraction techniques, as well as identify functional groups serving as marker.

Chemotaxonomy of Southeast Asian Peperomia (Piperaceae) Using High-Performance Thin-Layer Chromatography Colour Scale Fingerprint Imaging and Gas Chromatography–Mass Spectrometry

The morphological characters of Southeast Asia’s indigenous Peperomia species are very similar, especially in their flower structures. The flowers are simple, hermaphrodite and lack a perianth. Therefore, many species are hard to distinguish using morphological characters alone. Here, we apply chemometric data for species identification and classification, gathered using multiwavelength detection combined with the colour scale High-Performance Thin-Layer Chromatography (HPTLC) fingerprinting procedure and chemical compounds determined by Gas Chromatography–Mass Spectrometry (GC-MS). Fourteen taxa were investigated using hexane, ethyl acetate and ethanol solvent extractions. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) were used with the colour scale fingerprints to classify the Peperomia species. The PCA and HCA using the chromatogram profile from hexane divided the taxa into six groups compared to the profile from ethyl acetate and ethanol, which each detected seven groups. The chromatogram from the combined dataset of all three solvents can differentiate all the species. The GC-MS data detected a total of 40 compounds from the hexane extract, and these differed among Peperomia species. This approach based on HPTLC fingerprinting and GC-MS analysis can therefore be used as a tool for authentication and identification studies of Peperomia species.

Chemical Characterization of Moringa oleifera Lam. from Six Growth Locations in Central of Java: An Initiation of Standardization `

Moringa oleifera Lam. is in high demand as a raw material for pharmaceutical constituents and dietary supplements, particularly for producing stunting supplements. However, recommendations regarding M. oleifera quality standards have not supported this claim as scientific evidence of its safety and effectiveness. This research intends to expand knowledge regarding the quality standards of M. oleifera leaves, particularly their chemical properties. Height-variant samples of M. oleifera were collected from six locations in the Central Java, Indonesia. A hierarchical cluster analysis (HCA) was performed to group the most each parameter's values. The parameters included water-soluble extract, ethanol-soluble extract, total ash content, acid-insoluble ash, total flavonoid content was quantified as quercetin, and thin-layer chromatography (TLC) fingerprint. The suggestion of specific value for each parameter as a future reference for M. oleifera, including water- and ethanol-soluble extract content of at least 41% and 19%, and a total- and acid-insoluble ash content of a maximum of 11% and 4%, respectively. The minimal quercetin contains as the chemical marker compound of M. oleifera was a minimum of 1.285%. The chromatogram profile of TLC suggested 7 (254 nm) and 8 (366 nm) spots.

Kinetic optimization of angkak – red ginger extraction and its impact on antioxidant activity

A mixture of angkak and red ginger is a Chinese herbal concoction with potential as an antioxidant. This herbal combination can be extracted using the kinetic hot maceration method by optimizing the stirring speed to be more efficient. Previous studies have shown that using 400, 600, and 800 rpm stirring speeds provides the best IC50 and yield. Based on these problems, this research aims to determine the effect of increasing stirring speed on IC50 and % yield in the extraction of angkak and red ginger, as well as prove the benefits of this herbal combination through the IC50 value. The method used is extracting a mixture of angkak and red ginger at kinetic variations of 400, 600, and 800 rpm using a magnetic hotplate stirrer at a temperature of 60ºC for 2 hours; determination of % yield; determining the chromatogram profile using TLC; determination of antioxidant activity using DPPH; as well as data analysis of percentage yield and IC50 using SPSS. Analysis using SPSS shows that the stirring speed significantly affects the % yield, where increasing the moving speed above 400 rpm results in a decrease in the % yield. However, the moving speed does not affect the IC50. The TLC chromatogram profile shows the presence of 6-gingerol compounds in angkak and red and yellow pigment compounds in red ginger, which have potential as antioxidants. The optimization results in this study obtained optimal % yield and IC50 at 400 rpm with average values, respectively, namely 15.933 ± 3.4771% and 103.76 ± 10.032 ppm, and the combination of angkak and red ginger ingredients can work synergistically to increase yield and antioxidant.

Potential of Endophyctic Fungi Isolates IFAZ-6 from Bidara Root (Ziziphus mauritiana Lam.) as Antibacterials Againts Digestive Tract Infection

Bidara root (Ziziphus mauritiana Lam.) has activity as an antibacterial against digestive tract infections. This study aims to determine the antibacterial activity of isolate IFAZ-6 endophytic fungi of bidara root against bacteria that cause digestive tract infections using the TLC-Bioautography method. Purification was conducted on the active isolate IFAZ-6 to obtain a pure endophytic fungi isolate. Subsequently, macroscopic and microscopic observations were carried out. Then, fermentation was performed in MYB (Maltose Yeast Broth) medium for 21 days. Screening and evaporation were conducted to produce an extract. The obtained extract was subjected to TLC analysis using a chloroform: methanol eluent (7:1). The TLC-Bioautography test was conducted against Escherichia coli, Salmonella typhi, Shigella dysentriae, and Vibrio cholerae bacteria. The TLC identification results showed one spot with an Rf value of 0.80 for the IFAZ-6 isolate. The chromatogram profile obtained from the TLC-Bioautography method for the IFAZ-6 isolate demonstrated antibacterial activity against bacteria that cause digestive tract infections.

Antibiotics and probiotics-induced effects on the total fatty acid composition of feces in a rat model

Fatty acids (FAs) play important roles as membrane components and signal transduction molecules. Changes in short chain FA (SCFA) composition are associated with gut microbiota modifications. However, the effect of bacteria-driven changes on the detailed FA spectrum has not been explored yet. We investigated the effect of antibiotics (ABx) and/or probiotics, in four treatment groups on rat stool FA composition. Principal component analysis indicated that the chromatogram profiles of the treatment groups differ, which was also observed at different time points. Linear mixed effects models showed that in the parameters compared (sampling times, treatments. and their interactions), both the weight percentage and the concentration of FAs were affected by ABx and probiotic administration. This study found that the gut microbiome defines trans and branched saturated FAs, most saturated FAs, and unsaturated FAs with less carbon atoms. These results are among the first ones to demonstrate the restoring effects of a probiotic mixture on a substantial part of the altered total FA spectrum, and also revealed a previously unknown relationship between gut bacteria and a larger group of FAs. These findings suggest that intestinal bacteria produce not only SCFAs but also other FAs that may affect the host’s physiological processes.

Contents of an Early Byzantine Amphora from Kruglaya Bay, the Black Sea

A fragment of an amphora found in the Kruglaya Bay near Sevastopol was filled with an unknown brown substance with a strong smell of tar. On the vessel’s neck, there is a round stamp ~30 mm in diameter, depicting the bust of an emperor encircled by an inscription. It resembles stamps on amphorae made in Alexandria and the Geronisos Island. The inscription reads, “επί Πτολεμαίου επάρχου”. The gas chromatography–mass spectrometry analysis revealed dehydroabietic acid, methyl dehydroabietate, norabietatrienes, retene, and other phenanthrene derivatives, suggesting that the substance resulted from dry distillation of wood of the Pinaceae family. The headspace analysis yielded components of turpentine oil such as α-pinene, camphene, limonene, cymenes, and others terpenes. To establish the sample’s origin, the amphora’s content was compared with modern pinewood tar obtained by the traditional method. Given nearly identical chromatogram profiles of the amphora’s contents and of tar in areas relating to resin acids, similar values of peak areas of biomarker components, and the presence of turpentine oil components in the sample, it is highly probable that the amphora indeed contained tar.

Chromatogram profile of andrographolide-glucuronate and andrographolide glycine in rabbit feces extract

Chromatogram profile of andrographolide-glucuronate and andrographolide glycine in rabbit feces extract

Morphology Profile, Anatomy and Phytochemistry of Beluntas Leaves (Pluchea indica)

Beluntas (Pluchea indica), is one of species in Asteraceae family, traditionally used to increase appetite, to treat indigestion, rheumatism and body odor problems. This study aims to provide morphological and anatomical profiles of Beluntas leaves which are often used in medicine, to determine the chemical content of Beluntas leaves using phytochemical screening tests and thin layer chromatografic profiles, and also to determine flavonoids, polyphenols and alkaloids contents. The observation results showed Beluntas leaves are an oval leaf shape with pinnate leaf reinforcement. Anatomical observation of the leaves, found that the leaf type is equifacial with collateral vascular bundles, the presence of non glandulair multicellular trichomes with constricted in the cross wall, papillae and glandular Compositae trichomes. Tube method results and thin layer chromatography profiles showed Beluntas leaves contained polyphenols, saponins, alkaloids, flavonoids, steroids and essential oils. The chromatogram profile with infrared spectrophotometry shows the wave numbers in the fingerprint region 1515 – 1516, 1259 – 1260, 1159 – 1162, 1112 – 1115, 1046 – 1048, 811 – 812, 505 – 506 and 452 – 454 cm-1. The total amounts of polyphenols, flavonoid and alkaloid contained in Beluntas leaves were 3.1 - 7.7% w/w, 0.5 - 1.2% w/w and 0.03 – 0.08% w/w, respectively.

THE EFFECT OF VARIOUS LOCATION AND SOLVENT TO SAMBILOTO (Andrographis paniculata (Burm.f.) Nees) PHYTOCHEMICAL PROFILES

Background: Sambiloto (Andrographis paniculata (Burm.f.) Nees) is one of the traditional medicines that contain multiple phytochemical compounds. Phytochemical compounds can be influenced by growing location. Every location has its characteristic that affects the metabolic process of phytochemical compounds. Apart from that, the extraction solvent also plays an essential role in attracting compounds in sambiloto. The solvent is related to the polarity and can affect the phytochemical profile. Objective: This research aims to determine the effect of the growing location and extraction solvent of sambiloto on its phytochemical profile. Methods: Sambiloto from Denpasar (Dps), Sukoharjo (Skj), and Sleman (Slm) was extracted (1:10 w/v) using ultrasonic-assisted extraction in methanol 100% (MeOH100) and methanol 50% (MeOH50). In water solvents, extraction is carried out using the infusion method. Fingerprint analysis using HPTLC-Densitometry. The data obtained was analyzed further using principal component analysis (PCA). Results: Fingerprint analysis obtained peak data that varied in each extraction solvent. There are four major peaks in the chromatogram profile of each solvent. The peak chromatogram for each solvent also shows differences at each location. PCA analysis shows that the phytochemical content of sambiloto extract is divided into three main clusters. The distribution of the clusters is based on variations in the extraction solvent. Variations in growing location for each sambiloto also influence its phytochemical profile. However, these variations are still in the same quadrant for each solvent. Conclusion: The solvent determines more variations in the phytochemical profile of sambiloto extract than the growing location.
 Keywords: Sambiloto; Solvent; Location; Phytochemical; Principal Component Analysis

Pengujian Toksisitas dan Antioksidan Ekstrak Etilasetat dan n-Butanol Hipokotil Sarang Semut, Myrmecodia pendens Merr. & Perry

Sarang semut, Myrmecodia pendens Merr. & Perry (Rubiaceae) is an epiphytic plant from Papua which is used to cure various diseases. The purpose of this study is to see the chromatogram profile [Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC)] analysis, and biological test of ethyl acetate and n-butanol of Sarang semut’s hypocotyl extracts from methanol extracts. Meanwhile, the biological tests carried out were toxicity tests on Artemia salina L shrimp larvae and antioxidant assay with free radical scavenging methods (DPPH). The results of the antioxidant activity test showed that the antioxidant activity of the ethyl acetate extract was higher (IC50=18.23μg/ml) than the n-butanol extract (IC50=29.58μg/ml). The results of the toxicity test on the ethyl acetate extract were higher (LC50=101.84 g/ml) than the n-butanol extract (LC50=154.59 g/ml). TLC analysis results showed that the mobile phase for the best separation of chemical compounds in ethyl acetate extract was chloroform-methanol (10:1) and n-butanol extract was the mobile phase chloroform-methanol-water (5:5:1), while HPLC analysis showed that the mobile phase for the separation of chemical compounds in the ethyl acetate extract was n-hexane-ethyl acetate (10:1) and the n-butanol extract was methanol-water (10:1).

Immunostimulant Compounds Identification in Indonesian Underutilized Zingiberaceae Spices

Spices are known for its health beneficial effect beside its function as flavouring and condiment. This study aimed to screen immunostimulant activities of 12 underutilized species of Zingiberaceae grown in Indonesia. The compound associated with immunostimulant activity in the most active sample was identified by linking the chromatogram profile of hexane, chloroform, ethyl acetate, methanol, and water fractions of the selected spices to its activity using orthogonal projection to the least square (OPLS) analysis, followed by LC-MS characterization. The result showed that Alpinia malaccensis and Zingiber ottensii had the highest lymphocyte cell proliferation index (SI) at concentrations of 400 ppm. A. malaccensis was chosen for the next step. The results of OPLS analysis showed that the retention time interval which strongly correlated with the immunostimulant activity was 27.0 – 28.5 minutes, which were abundant in methanol fraction. The mass spectra analysis of methanol fraction using UHPLC-HRMS showed that the respective retention time was attributed to flavokawain B. This compound was reported to increase rat splenocyte proliferation in vivo.

Influence of chemical structure of SBr on the interference to gasoline identification for fire debris analysis

To examine the relationship between the molecular composition of interferents and their interference on gasoline identification in fire debris analysis, we conducted free burning experiments using five polystyrene-butadienes (SBrs) with styrene contents ranging from 9.5 wt% to 40 wt%. The combustion residues of these SBrs with and without the presence of gasoline were analyzed via gas chromatography-mass spectrometry. The compounds frequently used for gasoline identification, were detected in all the SBr combustion residues, and the Pearson product moment correlation coefficients have been calculated to quantify the interference extent based on the relative peak area ratios of the characteristic compounds. When gasoline is loaded onto these SBrs, particular attention should be paid since the relative content of the compounds of interest changed significantly, which distorted the chromatogram profile of gasoline greatly. Combining the above analysis results, we can confirm that materials containing “alkylbenzene” in their molecular structure are likely to result in both false positive and false negative identifications when determining the presence of ignitable liquid. Additionally, the interference extent varies with the molecular composition of the interferents. This further emphasizes the importance of considering the molecular structure when assessing the interference caused by these materials.

Identification of volatile compounds in several meat and bone broth using Solid Phase Micro Extraction-Gas Chromatography Mass Spectrometry (SPME-GCMS) for initial detection of Halal and Non-Halal Food

The development of techniques for detecting halal and non-halal animal meat is of great importance as a useful tool for monitoring and assuring food quality for Muslim. Flavor or aroma is one of the sensory attributes for consumers to distinguish broth cattle. The differences aroma of broth cattle is contributed by the content of volatile compounds. This study was aimed to determine the composition of volatile compounds in meat and bone broth from five different animals, i.e., cow (beef), pig (pork), goat, lamb, and chicken for initial identification of halal and non halal food. A preliminary test for samples of meat and bone broth was done by identifying their physical properties including pH, viscosity, and density. The volatile compounds in meat and broth samples were extracted using a solid phase-micro extraction (SPME) at room temperature and analyzed by gas chromatography-mass spectrometry (GCMS). The results revealed the physical properties of meat and bone broth, i.e., pH, viscosity, and density, cannot be used to distinguish the type of broth produced from beef, pork, goat, lamb, and chicken. Successful results for the identification were achieved through chromatogram profile of volatile compounds from meat and bone broth which show characteristics to each type of animal, and therefore it can be used to distinguish pork from beef, goat, lamb, and chicken as initial detection for halal and non halal food. The research also identified 7 predominant volatile compounds as a marker for meat pork and other 8 specific compounds marker for pork bone.

Aktivitas Antioksidan Ekstrak Air Kombinasi Kulit Pisang Kepok (Musa paradisiaca L.) dan Nanas (Ananas comosus L.) dengan Metode DPPH dan FRAP

Banana kepok (Musa paradisiaca L.) and pineapple (Ananas comosus L.) are types of fruit that are widely consumed by the community. However, the skin is only disposed of as waste, where the waste of the fruit skin reaches 30% of the whole fruit. Kepok banana peels and pineapples contain phenols and flavonoids which are proven to function as antioxidants. This study aims to determine the IC50 value of the combined sample using the DPPH and FRAP methods. The extract samples were divided into 2 groups, namely the combination of kepok banana peel extract and pineapple with a ratio of 1:1 and 1:3. Extraction is done by infusion method. Analysis of the content of phenolic and flavonoid compounds was carried out by the TLC method on single samples of kepok banana peels and pineapple peels. Antioxidant activity test was carried out by 2 methods, namely DPPH and FRAP. The yield of the extract in the 1:1 combination was found to be 48% and the 1:3 combination was 47%. The chromatogram profile showed that each single sample was positive for phenols and flavonoids. The results of the analysis of the antioxidant activity of the DPPH method showed the IC50 value in the 1:1 combination group was 1188.27 ppm while in the 1:3 combination group it was 740.98 ppm. In the FRAP method, the IC50 value in the 1:1 combination group was 1251.85 ppm, while in the 1:3 combination group it was 2218.94 ppm. Based on the results of the test, there are differences in the measurement of antioxidant activity with 2 different methods based on the antioxidants action that contains in the sample, so that the more recommended method is DPPH based on the type of antioxidant compounds contained in the sample.

Total flavonoid of dry extract and fraction of selected shallot (Allium ascalonicum L.) using ultraviolet-visible spectrophotometry and HPLC

Shallots (Allium ascalonicum L.) of Brebes are plants with a high flavonoid content. Furthermore, the flavonoids in shallots were extracted using the proper methods and solvents. In this study, shallot simplicia was dried at 30, 40, 50, and 60°C before being macerated in a 70% ethanol solvent. Water, ethyl acetate, and n-hexane were used as solvents to fractionate the ethanol extract. Ultraviolet visible (UV-Vis) spectrophotometry was used to determine the total flavonoid levels in the extract. The best results were obtained from HPLC readings of the quercetin chromatogram profile. The ethanol extract contained the most flavonoids, with 13.484 mg EQ/g after drying at 40°C

Evaluating multiproduct chromatography Protein A resinreuse for monoclonal antibodies in biopharmaceutical manufacturing.

In good manufacturing practice (GMP) facilities in the biopharmaceutical industry, chromatography resins are largely underutilized during purification of single drug products during clinical production. Chromatography resins are dedicated to a specific product and disposed of, after only a fraction of their lifetime due to concerns of potential product carryover from one program to another. In this study, we follow a resin lifetime methodology typically used for commercial submissions and apply it to determine the feasibility of purifying different products on a Protein A MabSelect PrismA™ resin. Three distinct monoclonal antibodies were used as model molecules. Column performance was monitored through chromatogram profiles, yield, clearance capability of selected media components, pressure and product quality. A protein carryover study was designed to demonstrate that the column cleaning procedures reduced protein carryover to safe cleanliness levels regardless of multiple product contact cycles and the order in which the mAbs are captured. Data show that up to 90 total cycles (30 cycles per antibody), there was negligible protein carryover and impact on process performance. Product quality was consistent, with the only meaningful trends found for the leached Protein A ligand, without affecting the conclusion of the study. While the study was restricted to three antibodies, the proof of concept for resin reuse was demonstrated.

Extraction, physicochemical properties, and antioxidant activity of natural melanin from Auricularia heimuer fermentation.

Natural melanin from Auricularia heimuer have numerous beneficial biological properties, which were used as a safe and healthy colorant in several industries. In this study, single-factor experiments, Box-Behnken design (BBD), and response surface methodology (RSM) were employed to investigate the effects of alkali-soluble pH, acid precipitation pH, and microwave time on the extraction yield of Auricularia heimuer melanin (AHM) from fermentation. Ultraviolet-visible spectrum (UV-Vis), Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), and high-performance liquid chromatography (HPLC) were used to analyze the extracted AHM. The solubility, stability, and antioxidant activities of AHM were also measured. The results showed that alkali-soluble pH, acid precipitation pH, and microwave time significantly affected the AHM yield, with the following optimized microwave-assisted extraction conditions: alkali-soluble pH of 12.3, acid precipitation pH of 3.1, and microwave time of 53 min, resulting in an AHM extraction yield of 0.4042%. AHM exhibited a strong absorption at 210 nm, similar to melanin from other sources. FT-IR spectroscopy also revealed that AHM exhibited the three characteristic absorption peaks of natural melanin. The HPLC chromatogram profile of AHM showed a single symmetrical elution peak with a 2.435 min retention time. AHM was highly soluble in alkali solution, insoluble in distilled water and organic solvents, and demonstrated strong DPPH, OH, and ABTS free radical scavenging activities. This study provides technical support to optimize AHM extraction for use in the medical and food industries.

Sevoflurane Exposure of Clinical Doses in Pregnant Rats Induces Vcan Changes without Significant Neural Apoptosis in the Offspring

Background and Objectives: Sevoflurane is a commonly used inhalational anaesthetic in clinics. Prolonged exposure to sevoflurane can induce significant changes in lipid metabolism and neuronal damage in the developing brain. However, the effect of exposure of pregnant rats to clinical doses of sevoflurane remains unclear. Materials and Methods: Twenty-eight pregnant rats were randomly and equally divided into sevoflurane exposure (S) group, control (C) and a blank group at gestational day (G) 18; Rats in S group received 2% sevoflurane with 98% oxygen for 6 h in an anesthetizing chamber, while C group received 100% oxygen at an identical flow rate for 6 h in an identical chamber. Partial least squares discriminant analysis (PLS-DA), ultra performance liquid chromatography/time-of-flight mass spectrometry(UPLC/TOF-MS) and MetaboAnalyst were used to analysis acquire metabolomics profiles, and immunohistochemical changes of neuronalapoptosis in hippocampus and cortex of neonatal rats were also analyzed. Results: This study aimed to explore lipidomics and transcriptomics changes related to 2% sevoflurane exposure for 6 h in the developing brains of newborn offspring rats. Ultra-performance liquid chromatography/time-of-flight mass spectrometry (UPLC/TOF-MS) and RNA sequencing (RNA-seq) analyses were used to acquire metabolomics and transcriptomics profiles. We used RNA-seq to analyse the expression of the coding and non-coding transcripts in neural cells of the cerebral cortex. No significant differences in arterial oxygen tension (PaO2), arterial carbon dioxide tension (PaCO2), or arterial blood gas were found between the groups. The relative standard deviation (RSD) of retention times was <1.53%, and the RSDs of peak areas ranged from 2.13% to 8.51%. Base peak chromatogram (BPC) profiles showed no differences between the groups. We evaluated the partial least square-discriminant analysis (PLS-DA) model. In negative ion mode, R2X was over 70%, R2Y was over 93%, and Q2 (cum) was over 80%. Cell apoptosis was not remarkably enhanced by TUNEL and haematoxylin and eosin (HE) staining in the sevoflurane-exposed group compared to the control group (p > 0.05). Glycerophospholipid (GP) and sphingolipid metabolism disturbances might adversely influence neurodevelopment in offspring. The expression of mRNAs (Vcan gene, related to neuronal development, function and repair) of the sevoflurane group was significantly increased in the differential genes by qRT-PCR verification. Conclusions: GP and sphingolipid metabolism homeostasis may be potential therapeutic approaches against inhalational anaesthetic-induced neurodegenerative disorders. Meanwhile, sevoflurane-induced Vcan changes indicated some lipidomic and transcriptomic changes, even if neural cell apoptosis was not significantly changed in the usual clinical dose of sevoflurane exposure.