This study evaluated the effect of feeding milk replacers (MR) formulated with alternative fat sources and containing different fat compositions on plasma cholesterol of dairy calves. Forty-five individually housed male Holstein calves (2.3 ± 0.82 d; mean ± SD) were blocked by arrival day and age. Within each block, calves were randomly assigned to one of 3 treatments: 1) an MR containing a fat blend with 65% rapeseed and 35% coconut fats (RC), 2) an MR containing 65% palm and 35% coconut fat (PC), and 3) an MR containing 65% lard and 35% dairy cream (LD). All MR were isoenergetic with 30% fat, 25% protein, and 36% lactose (DM basis). Calves were fed 6.0 L/d from d 1 to 5, 7.0 L/d from d 6 to 9, and 8.0 L/d from d 10 to 35 at 13.5% solids. Water and chopped wheat straw were available ad libitum from arrival onwards. Milk, water, and straw intakes were recorded daily. Blood was sampled and BW was measured at arrival and weekly thereafter at 1300 h. Fecal scores were recorded daily after the morning meal. Growth and intakes did not differ among treatments. Plasma total cholesterol, high-density lipoprotein (HDL)-cholesterol, and low-density lipoprotein (LDL)-cholesterol concentrations were higher in calves fed RC than PC and LD, and higher in calves fed PC than LD. Additionally, plasma TG was greater in calves fed PC than LD at wk 2 after arrival, while calves fed RC did not differ from the other treatments. The proportion of abnormal fecal scores were reduced in calves fed RC in wk 2. Including palm and rapeseed fats in the MR did not affect growth but increased plasma total cholesterol.