Method Of Choice Research Articles

LC-MS investigated as a tool to study the metabolomic characteristics of cereal fermentation

Fermentation of cereals to obtain desired food products is an age-old technique. It is often used to enhance the acceptability and shelf-life of the product. Fermentation can be viewed as a process where microbes bring about metabolic changes in the overall metabolic constitution of the product. In recent years, food metabolomics is emerging as a field to decipher these metabolomic changes to develop a more sustainable and robust food industry. With the advent of advanced analytical platforms, it is now possible to analyse cereal fermentation in-depth in a high-throughput manner. One such tool is LC-MS which has now become an indispensable tool for such food-based studies. The dynamic coverage of metabolites along with high qualitative and quantitative reproducibility makes it a method of choice in many metabolomic studies. The present study undertakes an exhaustive survey of the scholarly literature from 2005 to 2023 focusing on key studies that employ the LC-MS platform to study fermentation, particularly in cereals. The findings strongly advocate for the versatility and utility of LC-MS especially when complemented with chemometric approach in unravelling the intricacies of cereal fermentation. By leveraging these insights, the food industry can further enhance the development of functional foods and foster innovation.

Assessing the functional impact of protein binding site definition.

Many biomedical applications, such as classification of binding specificities or bioengineering, depend on the accurate definition of protein binding interfaces. Depending on the choice of method used, substantially different sets of residues can be classified as belonging to the interface of a protein. A typical approach used to verify these definitions is to mutate residues and measure the impact of these changes on binding. Besides the lack of exhaustive data, this approach also suffers from the fundamental problem that a mutation introduces an unknown amount of alteration into an interface, which potentially alters the binding characteristics of the interface. In this study we explore the impact of alternative binding site definitions on the ability of a protein to recognize its cognate ligand using a pharmacophore approach, which does not affect the interface. The study also shows that methods for protein binding interface predictions should perform above approximately F-score = 0.7 accuracy level to capture the biological function of a protein.

Sensitivity of Standardized Precipitation and Evapotranspiration Index (SPEI) to the Choice of SPEI Probability Distribution and Evapotranspiration Method

Study RegionThe state of Oklahoma located in the Southern Plains region of the United States. Study FocusThe standardized precipitation evapotranspiration index (SPEI) is a widely used meteorological drought index that incorporates potential evapotranspiration (PET) into a precipitation-based index. However, the understanding of the appropriate PET method for SPEI across different temporal scales in non-arid climate conditions remains limited. We compared Thornthwaite (TW), Hargreaves (HG), and Penman-Monteith (PM) equations for SPEI at various accumulations, considering three temporal scales: 1) long-term (25 years), 2) event-based, and 3) monthly. Also, we examined the log-logistic and generalized extreme value distributions to test the normality of SPEI computed from the three PET methods. To do this, we utilized high-quality climate datasets measured at 107 stations across the state of Oklahoma, United States, which has a diverse climate ranging from semi-arid to humid subtropical. New Hydrological Insights for the RegionThe log-logistic distribution was found to be suitable for SPEI. The SPEI-HG showed better agreement with SPEI-PM than SPEI-TW in this region for the analyses of three temporal scales. However, for accumulations of SPEI longer than one year, both TW and HG equations showed no significant differences with SPEI-PM. The findings provide practical guidance for selecting an appropriate PET equation in the Southern Plains region depending on the purpose of study without resorting to data-intensive methods for PET estimation.

Dental age estimation methods applied to Indian children and adolescents: A systematic review and meta-analysis

Country-specific systematic reviews and meta-analyses have been proposed to compile the available literature and rank methods based on their performance for a target population. India is a country with a vast scientific literature on dental age estimation. This systematic review aimed to provide evidence to help the decision of experts regarding the method of choice for dental age estimation in India. The research protocol was registered in Open Science Framework. Literature Search was performed in Embase, LILACS, MedLine (via PubMed), SciELO, Scopus and Web of Science. Grey Literature was searched in Google Scholar and ProQuest. Observational cross-sectional studies that compared chronological and estimated ages using Demirjian (original [DEM] and Chaillet's modification [modified-DEM]) and Acharya (ACH) methods were included. JBI tool was used to assess the risk of bias. The search detected 9799 studies, out of which 56 were eligible (n=13,107 panoramic radiographs of Indian individuals). Low risk of bias was registered for 48 studies, while 8 presented a moderate risk of bias. The meta-analysis showed a standardized mean difference between chronological and estimated ages of -0.11 (95%CI: -0.29; 0.07), 0.74 (95%CI: 0.39; 1.09), and -0.01 (95%CI: -0.23; 0.22) years for DEM, modified-DEM and ACH, respectively. High heterogeneity (I2=88-93%) was observed across studies for all the methods, including subgroup analyses based on sex. This study ranked ACH, DEM and modified-DEM (from the best to the worse) performances in the Indian population.

Efficient estimation of true fixed-effects stochastic frontier models

AbstractFixed-effects modeling has become the method of choice in several panel data settings, including models for stochastic frontier analysis. A notable instance of stochastic frontier panel data models is the true fixed-effects model, which allows disentangling unit heterogeneity from efficiency evaluations. While such a model is theoretically appealing, its estimation is hampered by incidental parameters. This note proposes a simple and rather general estimation approach where the unit-specific intercepts are integrated out of the likelihood function. We apply the theory of composite group families to the model of interest and demonstrate that the resulting integrated likelihood is a marginal likelihood with desirable inferential properties. The derivation of the result is provided in full, along with some connections with the existing literature and computational details. The method is illustrated for three notable models, given by the normal-half normal model, the heteroscedastic exponential model, and the normal-gamma model. The results of simulation experiments highlight the properties of the methodology.

Appraisal of clinical practice guidelines and consensus statements on obstetric anaesthesia: a systematic review using the AGREE II instrument

ObjectivesDespite the publication of hundreds of trials on obstetric anaesthesia, the management of these conditions remains suboptimal. We aimed to assess the quality and consistency of guidance documents for obstetric...

Unveiling the landscape of post-keratoplasty keratitis: a comprehensive epidemiological analysis in a tertiary center.

The present study aimed to epidemiologically evaluate patients with infectious keratitis following corneal transplantation. This retrospective study analyzed medical records of patients who underwent keratoplasty from March 2014 to March 2022 at a tertiary center. A total of seventy-five patients were evaluated. The data were classified based on culture results, the type of microorganisms involved, treatment requirements, and the type of primary keratoplasty performed. Seventy-five patients were evaluated in this study, with a mean age of 45.9years (22-95years). The mean duration between the first surgery and the incidence of infectious keratitis was 1.43years, and most cases occurred in the first year (56.2%). Bacterial and fungal keratitis in 2.17%, 1.39%, and 1.26% of cases undergoing penetrating keratoplasty (PK), endothelial keratoplasty (EK), and anterior lamellar keratoplasty (ALK) occurred, respectively. Streptococcus viridans (9.3%) and Staphylococcus aureus (6.6%) had the highest prevalence. Across various smear and culture results (gram-positive, gram-negative, fungal, and negative culture), no significant differences were found in endophthalmitis rates (P = 0.797) and the necessity for tectonic grafts (P = 0.790). Similarly, the choice of surgical method (PK, ALK, EK) showed no significant impact on the need for tectonic grafts (P = 0.45) or the rate of endophthalmitis (P = 0.55). The incidence of keratitis after a corneal graft was 1.7%, with Streptococcus viridans and Staphylococcus aureus the most common microorganisms. The rate of endophthalmitis associated with post-keratoplasty keratitis was 0.053%. There was no correlation between the necessity for a tectonic graft or the incidence of endophthalmitis and the type of microorganisms involved.

Feasibility and safety of permanent left bundle branch area pacing in pediatric patients

Abstract Introduction Left bundle branch area pacing (LBBAP) is a promising pacing strategy with a low and stable pacing threshold. It maintains electrical and mechanical synchronization of the ventricles. Conduction system pacing (CSP) has evolved rapidly to become the pacing method of choice for many adult and pediatric patients. Recent studies have begun to demonstrate the feasibility and efficacy of CSP in pediatric patients. Nowadays, research articles about LBBAP in children are comparatively rare, therefore, research in this area is preferred. Limited data on LBBAP in pediatric patients (≤ 18 years old) are available. Purpose This study aims to describe the feasibility and safety of LBBAP in pediatric patients. Methods This study includes 4 patients: 1 male, and 3 females, respectively. The average age of the patients was 15.5±1.9 years and the weight was 51,5±17,6 kg. All patients were diagnosed with 3rd-degree atrioventricular (AV) block, in one case the total AV block occurred after previous radiofrequency ablation of the parahisian and right lateral accessory pathway. One patient had previously been implanted with an epicardial pacemaker and presented to our clinic with ventricular lead dysfunction. Pacing parameters were assessed during implantation, the next day after the procedure, and 1, 3 months follow-up. Echocardiography was performed to evaluate LV function and assess the approximate lead location and impact on tricuspid valve function. Results LBBAP was successfully achieved in all patients resulting in narrow QRS complexes (102±12.7ms) and right bundle branch block QRS morphology. To confirm left bundle branch area capture, peak left ventricular activation time (pLVAT) (84.5±4.1ms), V6-V1 (43.5±2.5ms) inter-peak interval, presence of left bundle potential, transition from non-selective to selective capture were recorded at during the implantation. The mean paced QRS duration was (108±13 ms). Pacing threshold and impedance remain stable (0.75±0.25V, and 615±50 Ohm, respectively). There was no major complication during the implanting process and follow-up. Conclusions LBBAP can be performed safely in children with narrow QRS complexes and can achieve stable pacing parameters.

Patient preference and acceptability of self-sampling for cervical screening in colposcopy clinic attenders: A cross-sectional semi-structured survey.

Low vaginal self-sampling has been pioneered as an important development to improve uptake of cervical screening globally. Limited research is available in specific patient groups in the UK exploring views around self-sampling to detect high-risk human papillomavirus (hrHPV) DNA. Therefore, we explored patient views to support development of a novel point-of-care self-sampling cervical cancer screening device, by undertaking a cross-sectional semi-structured questionnaire survey to explore preferences, acceptability, barriers and facilitators around self-sampling. Patients attending a colposcopy clinic, 25-64 years old, were invited to participate after having carried out a low vaginal self-sample using a regular flocked swab. Participants self-completed an anonymous 12-point questionnaire. Quantitative data were analysed in MS Excel and Graphpad Prism, and qualitative data with Nvivo. We recruited 274 patients with a questionnaire response rate of 76%. Acceptability of self-sampling was high (95%, n = 187/197; Cronbachs-α = 0.778). Participants were asked their choice of future screening method: a) low vaginal self-sampling, b) healthcare professional collected vaginal swab, c) cervical brush sample with healthcare professional speculum examination, or d) no preference. Preferences were: a) 37% (n = 74/198), b) 19% (n = 37/198); c) 9% (n = 17/198), and d) 35% (n = 70/198), showing no single option as a strong preference. Key motivators were: Test simplicity (90%, n = 170/190), speed (81%, n = 153/190) and less pain (65%, n = 123/190). Barriers included lack of confidence taking the sample (53%, n = 10/19), resulting in preference for a healthcare professional sample (47%, n = 9/19). Whilst self-sampling showed high acceptability, lack of strong preference for screening method may reflect that respondents attending colposcopy are already engaged with screening and have differing perception of cervical cancer risk. This group appear less likely to 'switch' to self-sampling, and it may be better targeted within primary and community care, focusing on under-screened populations. Any shift in this paradigm in the UK requires comprehensive education and support for patients and providers.

Viral Diagnosis in Psittacine Birds: A Scientometric and Systematic Review of 47 Years

The first reports of viruses in psittacine birds date back to the early 1970s. Here, we elucidate the differences among these previous studies and the advances achieved. The objective of this study was to carry out a comprehensive review using both scientometric and systematic methods to analyze the evolution of published studies on viruses in psittacine birds up to 2022. The search descriptors “virus”, “diagnosis”, and “Psittaciformes” were used to find the articles of interest for this study. A total of 118 articles were manually selected, and the scientometric data were organized using the software VOSviewer® version 1.6.18. The present review revealed that: (i) on average, 2.5 articles/year on the diagnosis of viral infection in psittacine birds were published since 1975; (ii) the most productive research groups are concentrated in three countries: Australia, the United States, and Germany; (iii) the most important virus in psittacine birds is the Circovirus, which causes psittacine beak and feather disease; (iv) the diagnostic method of choice is polymerase chain reaction (PCR); and (v) the most studied psittacine birds were those in the Psittacidae family that were kept in captivity.

THE SIGNIFICANCE OF THE EXPERIMENT IMPROVING THE METHODS OF FORENSIC EXAMINATION

The article considers the issues of conducting an expert experiment at several levels, identifies three main stages of the experiment and develops an algorithm for expert actions during each of these stages (the initial stage, the direct conduct of an expert experiment and the final stage), these proposals are supported by the results of questionnaire surveys conducted among practitioners (forensic experts). Based on the research results, it was proposed to include among the main conditions for conducting an expert experiment the choice of methods for controlling the experiment, in particular, the choice of methods for controlling independent variables.

Optical sensor reveals the hidden influence of cell dissociation on adhesion measurements

Cell adhesion experiments are important in tissue engineering and for testing new biologically active surfaces, prostheses, and medical devices. Additionally, the initial state of adhesion (referred to as nascent adhesion) plays a key role and is currently being intensively researched. A critical step in handling all adherent cell types is their dissociation from their substrates for further processing. Various cell dissociation methods and reagents are used in most tissue culture laboratories (here, cell dissociation from the culture surface, cell harvesting, and cell detachment are used interchangeably). Typically, the dissociated cells are re-adhered for specific measurements or applications. However, the impact of the choice of dissociation method on cell adhesion in subsequent measurements, especially when comparing the adhesivity of various surfaces, is not well clarified. In this study, we demonstrate that the application of a label-free optical sensor can precisely quantify the effect of cell dissociation methods on cell adhesivity, both at the single-cell and population levels. The optical measurements allow for high-resolution monitoring of cellular adhesion without interfering with the physiological state of the cells. We found that the choice of reagent significantly alters cell adhesion on various surfaces. Our results clearly demonstrate that biological conclusions about cellular adhesion when comparing various surfaces are highly dependent on the employed dissociation method. Neglecting the choice of cellular dissociation can lead to misleading conclusions when evaluating cell adhesion data from various sources and comparing the adhesivity of two different surfaces (i.e., determining which surface is more or less adhesive).

Case Report of Multiple Rectosigmoid Adenomatous Polyps and Its Management i.e. Transanal Endoscopic Microsurgery

Adenomatous polyps in the colorectal region are prevalent. On account of its rarity and effective excision using Transanal Endoscopic Microsurgery (TEM) with assistance from a Covidien Small Incision Laparoscopic System (SILS) Port, we present this case of an adenomatous polyp wherein the patient complaints of per rectal bleed. Most frequently asymptomatic, adenomatous polyps are the most prevalent. In the present case, five to six polyps were found in the rectosigmoid area during colonoscopy. Rectal bleeding, stomach pain, and changes in bowel function that have a high malignant potential are the most typical symptoms associated with polyps. Large rectal adenomas should only be treated with TEM at this time, and traditional Trans Endoscopic (EMR/ESD) should be stopped. Despite the availability of newer procedures like endoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD), TEM is still the method of choice. TEM is not recorded in our country up till now. This is the first time it is performed in our country. Keywords: Adenomatous Polyp; Transanal Endoscopic Microsurgery; Small Incision Laparoscopic System; Endoscopic Mucosal Resection; Endoscopic Submucosal Dissection

Anti-inflammatory action of new hybrid N-acyl-[1,2]dithiolo-[3,4-c]quinoline-1-thione

ABSTRACT Most of pharmaceutical agents display a number of biological activities. It is obvious that testing even one compound for thousands of biological activities is not practically possible. A computer-aided prediction is therefore the method of choice in this case to select the most promising bioassays for particular compounds. Using the PASS Online software, we determined the probable anti-inflammatory action of the 12 new hybrid dithioloquinolinethiones derivatives. Chemical similarity search in the World-Wide Approved Drugs (WWAD) and DrugBank databases did not reveal close structural analogues with the anti-inflammatory action. Experimental testing of anti-inflammatory activity of the synthesized compounds in the carrageenan-induced inflammation mouse model confirmed the computational predictions. The anti-inflammatory activity of the studied compounds (2a, 3a-3k except for 3j) varied between 52.97% and 68.74%, being higher than the reference drug indomethacin (47%). The most active compounds appeared to be 3h (68.74%), 3k (66.91%) and 3b (63.74%) followed by 3e (61.50%). Thus, based on the in silico predictions a novel class of anti-inflammatory agents was discovered.

Endoscopy in the diagnosis and treatment of gastroduodenal bleedings in patients with pancreatitis

The OBJECTIVE was to study the possibilities of endoscopy in the diagnosis and treatment of bleedings in patients with pancreatitis.METHODS AND MATERIALS. Based on the analysis of 135 patients with pancreatitis complicated by gastroduodenal bleeding, we determined the location and possibilities of endoscopic hemostasis. The article analyzes 2 groups of patients. The first group (64 patients with acute pancreatitis and gastroduodenal bleeding). The second group (71 patients with chronic pancreatitis complicated by gastroduodenal bleeding). The article shows features of endoscopic examination of patients with pancreatitis complicated by gastroduodenal bleeding.RESULTS. The leading method of stopping bleeding in these groups of patients is endoscopic hemostasis.CONCLUSION. The choice of endoscopic hemostasis method depends on the cause and nature of the bleeding. The developed technique of endoscopic hemostasis has reduced the mortality rate from gastroduodenal bleeding in patients with pancreatitis to 3 %.

Impact of low dose CT radiation

With the technological advancements, we have witnessed a proliferation of innovative and beneficial methods in the utilization of CT devices. The contemporary application of low-dose CT (LDCT), both in adults and children, is considered the gold standard due to its wide range of indications. The aim of this study is to delineate the positive and negative impacts that LDCT imposes on the human body. The routine use of LDCT in practice raises numerous questions regarding its harmfulness and influence. Diverse opinions exist within the scientific community regarding the association between LDCT and cancer development; therefore, it is exceptionally important to consider the population involved in the study, the characteristics of the radiation itself, and the various body parts exposed to radiation prior to its application. Despite the considerable number of conducted studies advocating for the use of LDCT, its utilization and the development of potential consequences cannot be completely excluded. That is why one of the necessary prerequisites for the professional use of CT as the method of choice in diagnostics is knowledge and understanding of scientific studies and their results in accordance with the modern development of CT devices. What remains crucial in everyday practice is that the knowledge used is always scientifically based and ultimately — reasonable.

Preoperative assessment of bone density for dental implantation: a comparative study of three different ROI methods

BackgroundDental cone beam computed tomography (CBCT) is commonly used to evaluate cancellous bone density before dental implant surgery. However, to our knowledge, no measurement approach has been standardized yet. This study aimed to evaluate the relationship between three different regions of interest (ROI) methods on cancellous bone density at the dental implant site using dental CBCT images.MethodsPatients’ dental CBCT images (n = 300) obtained before dental implant surgery were processed using Mimics (Materialise, Leuven, Belgium). At the potential implant sites, the rectangle, cylinder, and surrounding cylinder ROI methods were used to measure bone density. Repeated measures one-way analysis of variance was performed to compare the three ROI methods in terms of measurement results. Pearson correlation analysis was performed to identify the likely pair-wise correlations between the three ROI methods.ResultsThe density value obtained using the surrounding cylinder approach (grayscale value [GV],523.56 ± 228.03) was significantly higher than the values obtained using the rectangle (GV, 497.04 ± 236.69) and cylinder (GV,493 ± 231.19) ROI methods in terms of results. Furthermore, significant correlations were noted between the ROI methods (r > 0.965; p < 0.001).ConclusionsThe density measured using the surrounding cylinder method was the highest. The choice of method may not influence the trends of measurement results.Trial registrationThis study was approved by the Institutional Review Board of China Medical University Hospital, No. CMUH111-REC3-205. Informed consent was waived by the Institutional Review Board of China Medical University Hospital, CMUH111-REC3-205, owing to the retrospective nature of the study.

Intrusion Mechanics in Orthodontics

Various methods for incisor intrusion and molar intrusion are discussed in detail in this review. These techniques involve applying different forces and mechanics to move teeth vertically into the jawbone.For incisor intrusion, methods such as utility arches, Connecticut intrusion arches, Burstone intrusion arches, K sir Arch, and tip back springs are utilized. Each of these methods employs unique mechanics to achieve the desired vertical movement of the incisors.On the other hand, molar intrusion techniques include the use of Temporary Anchorage Devices (TADs), headgears, biteplanes, magnets, maxillary intrusion splints, Invisalign, and active vertical correctors. These methods provide various approaches to intrude molars by applying force in different ways.The choice of intrusion method depends on factors such as the specific orthodontic case, treatment goals, and patient anatomy. Each technique has its own advantages, limitations, and indications for use in clinical practice..The aim of this overview is to provide information for approaching the ways of intrusion.

TAKING A WALK ON THE HEART FAILURE SIDE: COMPARISON OF METABOLIC VARIABLES DURING WALKING AND MAXIMAL EXERTION

Abstract Background Although cardiopulmonary exercise testing (CPET) is the gold standard to assess exercise capacity, simpler tests (i.e. 6–minute walk test, 6MWT) are also commonly used. Aim of this study was to evaluate the relationship between cardiorespiratory parameters during CPET and 6MWT in a large, multicenter, heterogeneous population. Methods We included athletes, healthy subjects, and heart failure (HF) patients of different severity, including left ventricular assist device (LVAD) carriers, who underwent both CPET and 6MWT with oxygen consumption measurement. Results We enrolled 186 subjects (16 athletes, 40 healthy, 115 non–LVAD HF patients, and 15 LVAD carriers). CPET–peakV̇O2 was 41.0 [35.0–45.8], 26.2 [23.1–31.0], 12.8 [11.1–15.3], and 15.2 [13.6–15.6] ml/Kg/min in athletes, healthy, HF patients, and LVAD carriers, respectively (p&amp;lt;0.001). During 6MWT they used 63.5 [56.3–76.8], 72.0 [57.8– 81.0], 95.5 [80.3–109], and 95.0 [92.0–99.0] % of their peakV̇O2, respectively. None of the athletes, 1 healthy (2.5%), 30 HF patients (26.1%), and 1 LVAD carrier (6.7%), reached a 6MWT–V̇O2 higher than their CPET–peakV̇O2. Both 6MWT–V̇O2 and walked distance were significantly associated with CPET–peakV̇O2 in the whole population (R2=0.637 and R2=0.533, p=&amp;lt;0.001) but not in the sub–groups. This was confirmed after adjustment for groups. Conclusion The 6MWT can be a maximal effort especially in most severe HF patients and suggest that, in absence of prognostic studies related to 6MWT metabolic values, CPET should remain the first method of choice in the functional assessment of patients with HF as well as in sport medicine

Surgical methods of treatment for tibial diaphyseal fractures

Background. Bone fractures are a public health concern. In recent years, there has been an upward trend in body weight of people of all age groups. Obesity has long been thought to help protect against fractures, but recent studies have shown that for every 5 cm increase in waist circumference, the risk of any fracture is 3 % higher. The purpose: according to the meta-analysis of modern medical literature, to determine the main directions of surgical treatment for long bone fractures, their advantages, and disadvantages, including in overweight patients, the features of diaphyseal fracture fixation in overweight patients. Materials and methods. A meta-analysis of special literature from scientific databases was conducted: Cochrane Library, Scopus, National Library of Medicine — National Institutes of Health, ReLAB-HS Rehabilitation Resources Repository. One hundred and thirty articles were analyzed, from them 31 were selected, which, in our opinion, reflect the purpose of the study. Results. All methods of surgical fixation of fractures have their advantages and disadvantages. The frequency of nonunions caused by intramedullary fixation of the femoral shaft can reach 10 %, and varus/valgus and rotational deformities and shortening can also be observed. But the use of locking screws prevents the occurrence of most complications. When fixing the fractures with plates, the main complications are related to superficial and deep infections, which were more often observed in overweight patients. The analysis demonstrated that in overweight and obese patients, the main complicating factor is not the method for fixing the fracture zone, but factors related to the health of the patient himself. So, despite the fact that the results of treatment of fractures in patients with normal weight and obesity did not have a statistically significant difference, an increase in cardiovascular complications, exacerbation of chronic respiratory diseases was observed. Moreover, it is the presence of concomitant diseases that often makes surgical intervention impossible. Conclusions. There is a large amount of research on surgical methods of fixing tibial diaphyseal fractures, but data on the choice of fixation method in overweight and obese patients as a separate approach were not found. There are data on complicating factors of excess weight in the treatment of fractures and surgical interventions. Systematic studies related specifically to the algorithm for choosing the method of fracture fixation and complications have also not been found.